… After incubation under rotation for 2 h, the mixture was centrifuged at 15,000 g for 15 min
and the pellet was washed five times with PBS. The final pellet was resuspended as above in …

We have used a variety of immunocytochemical procedures to localize albumin in transit
through the capillary endothelium of the murine myocardium and thereby identify endothelial …

… between 2 and 22 h after infection. After binding of the virus in R medium for 2 h, unbound
virus was … After 4 h, intracellular viruses were mainly in the tubular structures, and, at 19 …

… The RNA from each sample was hybridized for 16 h at 550C, treated with RNase A, and
electrophoresed on a 6% polyacrylamide/7.5 M urea gel. Immunoblotting. Postnuclear …

… phos), pH 7 .2, for 48 h with several changes and applied to a Sephadex G-150 column, 1.5
x 55 cm, equilibrated in the same buffer (flow rate, 12-13 ml/h, fraction vol 3 ml). Two protein …

The nature of second messenger-responsive intracellular Ca2+ stores in neurons remains
open for discussion. Here, we demonstrate the existence in Purkinje cells (PCs) of …

… Apoptosis in H9c2 cells after 16-h simulated ischemia and 2-h simulated reperfusion was
significantly reduced by MMPSI in a concentration-dependent manner (IC 50 =0.5 μM); similar …

To examine the physiological role of the GLUT4/muscle-fat specific facilitative glucose
transporter in regulating glucose homeostasis, we have generated transgenic mice expressing …

… in the fed state or following a 16-h fast. Quadriceps, diaphragm, heart, and … 1 h and then
processed for frozen sectioning (18) with 4.8 pglml of rabbit anti-human GLUT4 IgG (19) for 1 h …

… -h fixation… h, followed by several 5-min washes with 1 ml PBS, and continued with a 1:500
in PBS Alexa 594 goat anti-guinea pig (Molecular Probes, Eugene, OR) conjugate stain for 1 h …