A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein

J Nakai, M Ohkura, K Imoto - Nature biotechnology, 2001 - nature.com
Recently, several groups have developed green fluorescent protein (GFP)-based Ca 2+
probes. When applied in cells, however, these probes are difficult to use because of a low signal…

Primary structure and functional expression from complementary DNA of a brain calcium channel

Y Mori, T Friedrich, MS Kim, A Mikami, J Nakai, P Ruth… - Nature, 1991 - nature.com
The primary structure of a voltage-dependent cal-cium channel from rabbit brain has been
deduced by cloning and sequencing the complementary DNA. Calcium channel activity …

Imaging cellular signals in the heart in vivo: Cardiac expression of the high-signal Ca2+ indicator GCaMP2

…, J Robbins, G Salama, J Nakai… - Proceedings of the …, 2006 - National Acad Sciences
Genetically encoded sensor proteins provide unique opportunities to advance the
understanding of complex cellular interactions in physiologically relevant contexts; however, …

Rings of negatively charged amino acids determine the acetylcholine receptor channel conductance

…, C Busch, B Sakmann, M Mishina, T Konno, J Nakai… - Nature, 1988 - nature.com
The structure–function relationship of the nicotinic acetylcholine receptor (AChR) has been
effectively studied by the combination of complementary DNA manipulation and single-…

Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts

…, K Sakamoto, D Ido, T Shiina, M Ohkura, J Nakai… - Nature, 2016 - nature.com
j–m, Expression of cell adhesion protein pan-cadherin (Cadherin) and gap junction protein
connexin 43 … We also appreciate the scientific advice of MA Laflamme, J. Chong and N. Saito. …

Primary structure and distribution of a novel ryanodine receptor/calcium release channel from rabbit brain

Y Hakamata, J Nakai, H Takeshima, K Imoto - FEBS letters, 1992 - Elsevier
The complete amino acid sequence of a novel ryanodine receptor/calcium release channel
from rabbit brain has been deduced by cloning and sequence analysis of the EDNA. This …

Enhanced dihydropyridine receptor channel activity in the presence of ryanodine receptor

J Nakai, RT Dirksen, HT Nguyen, IN Pessah, KG Beam… - Nature, 1996 - nature.com
… We thank RM Ridley for supplying the marmosets, J. Bashford and l, Bolton for photographic
assistance, and BJ Everitt for critical reading of the manuscript and preparation of the …

Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel

J Nakai, T Imagawa, Y Hakamata, M Shigekawa… - FEBS …, 1990 - Wiley Online Library
… The percent sequence identity of a segment corresponding to positions j-9 to j+9 is plotted
against j, where j represents the position number; deletions and insertions (see Fig. 1) have …

Cilia at the node of mouse embryos sense fluid flow for left-right determination via Pkd2

…, Y Asai, G Sasaki, JA Belo, H Sasaki, J Nakai… - Science, 2012 - science.org
Unidirectional fluid flow plays an essential role in the breaking of left-right (LR) symmetry in
mouse embryos, but it has remained unclear how the flow is sensed by the embryo. We …

[PDF][PDF] A top-down cortical circuit for accurate sensory perception

…, M Ohkura, A Yamanaka, Y Yanagawa, J Nakai… - Neuron, 2015 - cell.com
… (J) Examples of the spatiotemporal dynamics of cVSD responses. (K) S1 and M2 activities
(inset) before and after TTX application. (L) Summary of (K) (64.6% ± 7.0% of control, n = 8 …