Single-molecule methods have matured into powerful and popular tools to probe the complex
behaviour of biological molecules, due to their unique abilities to probe molecular structure…

Single-molecule FRET (smFRET) has become a mainstream technique for studying biomolecular
structural dynamics. The rapid and wide adoption of smFRET experiments by an ever-…

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor
compartment and fuse with an acceptor compartment. Common principles of budding and fusion …

… Lemke; Cargo transport through the nuclear pore complex at a glance. J Cell Sci 15 January
2021; 134 (2): jcs247874. … We thank all members of the Lemke group for critical discussion. …

The ideal fluorescent probe for bioimaging is bright, absorbs at long wavelengths and can
be implemented flexibly in living cells and in vivo. However, the design of synthetic …

We studied the coupled binding and folding of α-synuclein, an intrinsically disordered protein
linked with Parkinson's disease. Using single-molecule fluorescence resonance energy …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, …

Nuclear pore complexes are fundamental components of all eukaryotic cells that mediate
nucleocytoplasmic exchange. Determining their 110-megadalton structure imposes a …

The ability to visualize biomolecules within living specimen by engineered fluorescence
tags has become a major tool in modern biotechnology and cell biology. Encoding fusion …

… VanDelinder and members of the Lemke group for proofreading this manuscript and for
helpful discussions. The authors declare a conflict of interest, as some UAAs are covered by a …