Shown are human embryonic kidney (HEK293T) cells expressing Neuroligin-2 (NL2) constructs that were introduced into these cells via transient transfection. NL2 is typically expressed at inhibitory synapses and is implicated in various neuropsychiatric disorders, but how its structural domains and cellular localization contribute to these disorders is unclear. In this image, surface NL2 is in red and total NL2 is in green; nuclei are stained with DAPI (blue). Assessing differential surface expression after introducing various NL2 mutants into this system shed light on the trafficking and membrane localization of different NL2 variants. See the article by Saurabh Pandey et al. for more information. Cover image: Jun Li and Saurabh Pandey