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GAP-43 transgenic mice: dispersed genomic sequences confer a GAP-43- like expression pattern during development and regeneration

J Vanselow, E Grabczyk, J Ping, M Baetscher, S Teng and MC Fishman
Journal of Neuroscience 1 February 1994, 14 (2) 499-510; DOI: https://doi.org/10.1523/JNEUROSCI.14-02-00499.1994
J Vanselow
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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E Grabczyk
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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J Ping
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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M Baetscher
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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S Teng
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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MC Fishman
Developmental Biology Laboratory, Massachusetts General Hospital, Boston.
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Abstract

Using transgenic mice, we have examined the expression pattern conferred by regions of genomic GAP-43 coupled to beta-galactosidase. We demonstrate that gene constructions that include the GAP-43 5′- flanking region along with sufficient sequences of the first intron drive beta-galactosidase (lacZ) expression to mimic in many regards the complex spatial and temporal pattern of endogenous GAP-43 expression. Transgene expression reaches peak levels during development, and persists at high levels in particular adult brain regions, such as the hippocampus and olfactory bulb. The inclusion of a stretch of the first intron in the construction is necessary to prevent expression outside of the nervous system, indicating that some of the cell specificity of GAP-43 expression is due to suppression of expression in inappropriate tissues. Injury caused by sciatic nerve crush causes reexpression of the transgene in adult sensory and motor neurons. This genomic region of GAP-43, therefore, includes elements responsive to neuronal growth signals that regulate both development and regeneration.

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The Journal of Neuroscience: 14 (2)
Journal of Neuroscience
Vol. 14, Issue 2
1 Feb 1994
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GAP-43 transgenic mice: dispersed genomic sequences confer a GAP-43- like expression pattern during development and regeneration
J Vanselow, E Grabczyk, J Ping, M Baetscher, S Teng, MC Fishman
Journal of Neuroscience 1 February 1994, 14 (2) 499-510; DOI: 10.1523/JNEUROSCI.14-02-00499.1994

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GAP-43 transgenic mice: dispersed genomic sequences confer a GAP-43- like expression pattern during development and regeneration
J Vanselow, E Grabczyk, J Ping, M Baetscher, S Teng, MC Fishman
Journal of Neuroscience 1 February 1994, 14 (2) 499-510; DOI: 10.1523/JNEUROSCI.14-02-00499.1994
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