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Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements

GW Hoyle, EH Mercer, RD Palmiter and RL Brinster
Journal of Neuroscience 1 May 1994, 14 (5) 2455-2463; https://doi.org/10.1523/JNEUROSCI.14-05-02455.1994
GW Hoyle
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
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EH Mercer
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
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RD Palmiter
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
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RL Brinster
Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104.
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Abstract

The promoter region of the human dopamine beta-hydroxylase (DBH) gene was analyzed in transgenic mice to identify DNA sequences responsible for the tissue- and cell-specific expression of the gene. Transgenic mice were generated that carried the Escherichia coli lacZ gene under control of DBH promoter fragments between 0.6 and 5.8 kilobases (kb) in length. Sequences required for expression in adult and fetal noradrenergic neurons were located between 0.6 and 1.1 kb 5′ to the DBH transcriptional start site. Sequences in this region and farther upstream also directed expression to dopaminergic and noncatecholaminergic brain neurons that was repressed by negative elements elsewhere in the gene. The results indicate that the neuron- specific expression of the DBH gene is mediated by positive regulatory elements but that negative elements are required to restrict expression to the proper subset of neurons.

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The Journal of Neuroscience: 14 (5)
Journal of Neuroscience
Vol. 14, Issue 5
1 May 1994
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Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements
GW Hoyle, EH Mercer, RD Palmiter, RL Brinster
Journal of Neuroscience 1 May 1994, 14 (5) 2455-2463; DOI: 10.1523/JNEUROSCI.14-05-02455.1994

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Cell-specific expression from the human dopamine beta-hydroxylase promoter in transgenic mice is controlled via a combination of positive and negative regulatory elements
GW Hoyle, EH Mercer, RD Palmiter, RL Brinster
Journal of Neuroscience 1 May 1994, 14 (5) 2455-2463; DOI: 10.1523/JNEUROSCI.14-05-02455.1994
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