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Cloning and characterization of two related serotonergic receptors from the brain and the reproductive system of Aplysia that activate phospholipase C

XC Li, JF Giot, D Kuhl, R Hen and ER Kandel
Journal of Neuroscience 1 November 1995, 15 (11) 7585-7591; DOI: https://doi.org/10.1523/JNEUROSCI.15-11-07585.1995
XC Li
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
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JF Giot
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
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D Kuhl
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
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R Hen
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
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ER Kandel
Howard Hughes Medical Institute, College of Physicians and Surgeons of Columbia University, New York, New York 10032, USA.
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Abstract

Serotonin (5-HT) plays important roles in various behavioral and physiological processes in Aplysia californica. These include feeding, locomotion, circadian rhythm, learning and memory, synaptic plasticity, and synaptic growth. Serotonin modulates these various functions by interacting with different 5-HT receptor subtypes that are coupled to various second-messenger systems. We report here the isolation and characterization of the first two serotonergic receptors from Aplysia californica, Ap5-HTB1 and Ap5-HTB2, using a strategy based on the amino acid sequence homology among G-protein-coupled biogenic amine receptors. Ap5-HTB1 and Ap5-HTB2 are both intronless and highly homologous to each other, sharing 79.5% sequence identity at the amino acid level. Sequence comparison reveals that these receptors are 33.1 to 23.3% identical to the following 5-HT receptors: 5-HTdro1 > 5-HT6 > 5-HTlym > mouse 5-HT1B > 5-HTdro2A > mouse 5-HT7 > rat 5-HT2A. Both Ap5- HTB1 and Ap5-HTB2 encode functional 5-HT receptors. When expressed in cultured cells, these receptors stimulate phospholipase C in response to 5-HT in a dose-dependent manner. This stimulation can be blocked by specific 5-HT receptor antagonists. Using RT-PCR and Western blot analysis, we have detected these receptors in the CNS (Ap5-HTB2) and in the reproductive system (Ap5-HTB1). The nucleotide sequences of Ap5- HTB1 and Ap5-HTB2 were submitted to GenBank; the accession numbers are L43557 and L43558, respectively.

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The Journal of Neuroscience: 15 (11)
Journal of Neuroscience
Vol. 15, Issue 11
1 Nov 1995
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Cloning and characterization of two related serotonergic receptors from the brain and the reproductive system of Aplysia that activate phospholipase C
XC Li, JF Giot, D Kuhl, R Hen, ER Kandel
Journal of Neuroscience 1 November 1995, 15 (11) 7585-7591; DOI: 10.1523/JNEUROSCI.15-11-07585.1995

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Cloning and characterization of two related serotonergic receptors from the brain and the reproductive system of Aplysia that activate phospholipase C
XC Li, JF Giot, D Kuhl, R Hen, ER Kandel
Journal of Neuroscience 1 November 1995, 15 (11) 7585-7591; DOI: 10.1523/JNEUROSCI.15-11-07585.1995
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