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Motility and cytoskeletal organization of migrating cerebellar granule neurons

RJ Rivas and ME Hatten
Journal of Neuroscience 1 February 1995, 15 (2) 981-989; DOI: https://doi.org/10.1523/JNEUROSCI.15-02-00981.1995
RJ Rivas
Rockefeller University, New York, New York 10021–6399.
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ME Hatten
Rockefeller University, New York, New York 10021–6399.
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Abstract

To characterize CNS neuronal precursor migration along astroglial fibers, we examined the motility of the migratory leading process and cytoskeletal-based mechanisms of locomotion of early postnatal mouse cerebellar granule neurons in vitro. To visualize the surface motility of the leading process, granule neurons were labeled with the fluorescent lipophilic dye, PKH-26, and imaged by time lapse fluorescence microscopy. The motile behavior and cytoskeletal organization of the migrating neuron had several distinctive features. As the migrating neuron moved along the glial fiber, the leading process rapidly extended, projecting up to 40 microns, and retracted, withdrawing towards the cell soma. Broad lamellipodia were common along the entire length of the leading process, giving it a ruffled appearance. Within the cell soma, a cage-like distribution of microtubules encircled the nucleus and actin filaments formed a subcortical rim underneath the plasma membrane. Disruption of actin filaments with cytochalasin B inhibited migration, suggesting involvement of actin subunit assembly in neuronal migration. Both microtubules and actin filaments were heavily concentrated in the leading process; the leading process did not show the development of a distinct actin-rich domain at its tip.

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The Journal of Neuroscience: 15 (2)
Journal of Neuroscience
Vol. 15, Issue 2
1 Feb 1995
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Motility and cytoskeletal organization of migrating cerebellar granule neurons
RJ Rivas, ME Hatten
Journal of Neuroscience 1 February 1995, 15 (2) 981-989; DOI: 10.1523/JNEUROSCI.15-02-00981.1995

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Motility and cytoskeletal organization of migrating cerebellar granule neurons
RJ Rivas, ME Hatten
Journal of Neuroscience 1 February 1995, 15 (2) 981-989; DOI: 10.1523/JNEUROSCI.15-02-00981.1995
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