Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
Articles

Neuronal regulation of c-fos, c-jun, and junB immediate-early genes in rat adrenal medulla

M Pelto-Huikko, A Dagerlind, J Kononen, JM Lundberg, M Villar, J Koistinaho, R Bravo and T Hokfelt
Journal of Neuroscience 1 March 1995, 15 (3) 1854-1868; DOI: https://doi.org/10.1523/JNEUROSCI.15-03-01854.1995
M Pelto-Huikko
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A Dagerlind
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Kononen
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JM Lundberg
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M Villar
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J Koistinaho
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R Bravo
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T Hokfelt
Department of Biomedical Sciences, University of Tampere, Finland.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

We have applied in situ hybridization histochemistry, Northern analysis, and immunocytochemistry to study the regulation of the immediate-early gene (IEG) c-fos, c-jun, and junB mRNAs and the respective proteins in the rat adrenal medulla. Electrophoresis mobility shift assay was used to examine changes in AP-1 DNA binding activity. In nontreated rats the mRNA and protein levels for these three IEGs were low. Reflex stimulation of adrenal medulla elicited by a single capsaicin injection induced a rapid and marked elevation in the mRNA levels for these IEGs. Stimulation with nicotine also caused a drastic increase in the mRNA levels, whereas muscarine only induced moderate elevations. c-fos and c-jun were induced strongly in adrenaline cells and only weakly in noradrenaline cells. junB was upregulated mainly in adrenaline cells. The AP-1 DNA binding activity was low in control adrenals, whereas a marked increase was observed after nicotine treatment. Treatment of the animals with a nicotinic (chlorisondamine) or a muscarinic (atropine) receptor antagonist did not change the expression of IEGs studied. The combination of the two drugs, however elevated the mRNA levels for all three IEGs, especially for junB. Pretreatment of the rats with chlorisondamine alone or in combination with atropine diminished the capsaicin-induced increase in c-fos, whereas atropine alone was less efficient. Increase in c-jun mRNA was not affected by these drugs. The capsaicin-induced elevation of junB mRNA levels was not influenced by chlorisondamine or atropine alone, whereas both combined potentiated the effect of capsaicin. The present results demonstrate that neurotransmitters released from splanchnic nerve terminals induce expression of c-fos, c-jun, and junB in adrenal chromaffin cells which results in increased AP-1 DNA binding activity. Although stimulation of both nicotinic and muscarinic cholinergic receptors may mediate the induction of these IEGs, it is possible that also another neurotransmitter(s), in addition to ACh, released from splanchnic nerve terminals is involved in the regulation of their expression, especially of c-jun and junB.

Back to top

In this issue

The Journal of Neuroscience: 15 (3)
Journal of Neuroscience
Vol. 15, Issue 3
1 Mar 1995
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Neuronal regulation of c-fos, c-jun, and junB immediate-early genes in rat adrenal medulla
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
View Full Page PDF
Citation Tools
Neuronal regulation of c-fos, c-jun, and junB immediate-early genes in rat adrenal medulla
M Pelto-Huikko, A Dagerlind, J Kononen, JM Lundberg, M Villar, J Koistinaho, R Bravo, T Hokfelt
Journal of Neuroscience 1 March 1995, 15 (3) 1854-1868; DOI: 10.1523/JNEUROSCI.15-03-01854.1995

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Neuronal regulation of c-fos, c-jun, and junB immediate-early genes in rat adrenal medulla
M Pelto-Huikko, A Dagerlind, J Kononen, JM Lundberg, M Villar, J Koistinaho, R Bravo, T Hokfelt
Journal of Neuroscience 1 March 1995, 15 (3) 1854-1868; DOI: 10.1523/JNEUROSCI.15-03-01854.1995
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

  • Choice Behavior Guided by Learned, But Not Innate, Taste Aversion Recruits the Orbitofrontal Cortex
  • Maturation of Spontaneous Firing Properties after Hearing Onset in Rat Auditory Nerve Fibers: Spontaneous Rates, Refractoriness, and Interfiber Correlations
  • Insulin Treatment Prevents Neuroinflammation and Neuronal Injury with Restored Neurobehavioral Function in Models of HIV/AIDS Neurodegeneration
Show more Articles
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
(JNeurosci logo)
(SfN logo)

Copyright © 2022 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.