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Swelling-induced chloride currents in neuroblastoma cells are calcium dependent

S Basavappa, V Chartouni, K Kirk, V Prpic, JC Ellory and AW Mangel
Journal of Neuroscience 1 May 1995, 15 (5) 3662-3666; DOI: https://doi.org/10.1523/JNEUROSCI.15-05-03662.1995
S Basavappa
Department of Physiology, University of Oxford, United Kingdom.
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V Chartouni
Department of Physiology, University of Oxford, United Kingdom.
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K Kirk
Department of Physiology, University of Oxford, United Kingdom.
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V Prpic
Department of Physiology, University of Oxford, United Kingdom.
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JC Ellory
Department of Physiology, University of Oxford, United Kingdom.
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AW Mangel
Department of Physiology, University of Oxford, United Kingdom.
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Abstract

The effects of osmotic stress on chloride (CI-) currents in the human neuroblastoma cell line CHP-100 were evaluated. Following exposure to hypoosmotic solution, an increase in whole-cell CI- current was observed. This current was blocked by the CI- channel blocker 5-nitro-2- (3-phenylpropylamino)-benzoic acid (NPPB). In cells loaded with the CI- permeability marker 125I, exposure to hypoosmotic solution increased 125I efflux by 197 +/- 14% (n = 41, p < 0.05) over controls. This increase was sensitive to NPPB. Hypoosmotic stress also increased cytosolic calcium levels (Ca2+) in fura-2-loaded cells. Pretreatment with EGTA inhibited the increase in cytosolic Ca2+, 125I efflux, and whole-cell CI- current produced by hypoosmotic solution. Antagonists of N-, L-, and T-type Ca2+ channels did not alter stimulation in 125I efflux or cytosolic Ca2+ levels during osmotic stress. However, omega- conotoxin MVIIC, a P-type Ca2+ channel blocker, inhibited hypoosmotically activated whole-cell CI- currents and increases in cytosolic Ca2+. It is concluded that a Ca(2+)-dependent change in CI- permeability is activated in CHP-100 cells in response to osmotic stress.

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The Journal of Neuroscience: 15 (5)
Journal of Neuroscience
Vol. 15, Issue 5
1 May 1995
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Swelling-induced chloride currents in neuroblastoma cells are calcium dependent
S Basavappa, V Chartouni, K Kirk, V Prpic, JC Ellory, AW Mangel
Journal of Neuroscience 1 May 1995, 15 (5) 3662-3666; DOI: 10.1523/JNEUROSCI.15-05-03662.1995

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Swelling-induced chloride currents in neuroblastoma cells are calcium dependent
S Basavappa, V Chartouni, K Kirk, V Prpic, JC Ellory, AW Mangel
Journal of Neuroscience 1 May 1995, 15 (5) 3662-3666; DOI: 10.1523/JNEUROSCI.15-05-03662.1995
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