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Articles

A dominant role of acid pH in inflammatory excitation and sensitization of nociceptors in rat skin, in vitro

KH Steen, AE Steen and PW Reeh
Journal of Neuroscience 1 May 1995, 15 (5) 3982-3989; DOI: https://doi.org/10.1523/JNEUROSCI.15-05-03982.1995
KH Steen
Institut fur Physiologie I, Universitat Erlangen-Nurnberg, Germany.
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AE Steen
Institut fur Physiologie I, Universitat Erlangen-Nurnberg, Germany.
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PW Reeh
Institut fur Physiologie I, Universitat Erlangen-Nurnberg, Germany.
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Abstract

A major role of local acidosis in long lasting excitation and sensitization of cutaneous nociceptors has recently been demonstrated. In inflamed tissue, acid pH meets with a mixture of inflammatory mediators which, by themselves, stimulate nociceptors though being subject to profound tachyphylaxis. We have mimicked this condition in a rat skin-saphenous nerve preparation in vitro which allows direct application of chemicals to the isolated receptive fields at the corium side. Stimulant solutions used were CO2-saturated “synthetic interstitial fluid” (CO2-SIF, pH 6.1), and “inflammatory soup” (IS) in submaximal concentration containing bradykinin, 5-HT, histamine, prostaglandin E2 (all 10(-6) M in SIF at 38.5 degrees C and pH 7.0), and a combination made of CO2-saturated IS (CO2-IS, pH 6.1). Identified mechano-heat sensitive (“polymodal”) C-fiber terminals (n = 36) were treated with these solutions for 5 min at 10 min intervals or for 30 min of sustained stimulation: 20 units responded to CO2-SIF, 12 to IS, whereas 27 units (75%) were excited by CO2-IS. Thus, 6 out of 15 units insensitive to either of the two basic solutions were stimulated by their combination. This enhanced effect of CO2-IS was also expressed in shorter latencies (than with CO2-SIF) and in a significantly larger mean response magnitude of the fiber population: 152 spikes with the combination versus 45 spikes evoked by IS and 93 spikes by CO2-SIF (n = 25; p < 0.002 and < 0.02, respectively, Wilcoxon test).

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The Journal of Neuroscience: 15 (5)
Journal of Neuroscience
Vol. 15, Issue 5
1 May 1995
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A dominant role of acid pH in inflammatory excitation and sensitization of nociceptors in rat skin, in vitro
KH Steen, AE Steen, PW Reeh
Journal of Neuroscience 1 May 1995, 15 (5) 3982-3989; DOI: 10.1523/JNEUROSCI.15-05-03982.1995

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A dominant role of acid pH in inflammatory excitation and sensitization of nociceptors in rat skin, in vitro
KH Steen, AE Steen, PW Reeh
Journal of Neuroscience 1 May 1995, 15 (5) 3982-3989; DOI: 10.1523/JNEUROSCI.15-05-03982.1995
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