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Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport

D Sulzer, TK Chen, YY Lau, H Kristensen, S Rayport and A Ewing
Journal of Neuroscience 1 May 1995, 15 (5) 4102-4108; DOI: https://doi.org/10.1523/JNEUROSCI.15-05-04102.1995
D Sulzer
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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TK Chen
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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YY Lau
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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H Kristensen
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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S Rayport
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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A Ewing
Department of Psychiatry, Columbia University, New York, New York 10032, USA.
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Abstract

Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole-cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from PC12 cells by > 50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport, and dopamine release.

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The Journal of Neuroscience: 15 (5)
Journal of Neuroscience
Vol. 15, Issue 5
1 May 1995
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Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport
D Sulzer, TK Chen, YY Lau, H Kristensen, S Rayport, A Ewing
Journal of Neuroscience 1 May 1995, 15 (5) 4102-4108; DOI: 10.1523/JNEUROSCI.15-05-04102.1995

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Amphetamine redistributes dopamine from synaptic vesicles to the cytosol and promotes reverse transport
D Sulzer, TK Chen, YY Lau, H Kristensen, S Rayport, A Ewing
Journal of Neuroscience 1 May 1995, 15 (5) 4102-4108; DOI: 10.1523/JNEUROSCI.15-05-04102.1995
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