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Increase of extracellular corticotropin-releasing factor-like immunoreactivity levels in the amygdala of awake rats during restraint stress and ethanol withdrawal as measured by microdialysis

EM Pich, M Lorang, M Yeganeh, F Rodriguez de Fonseca, J Raber, GF Koob and F Weiss
Journal of Neuroscience 1 August 1995, 15 (8) 5439-5447; DOI: https://doi.org/10.1523/JNEUROSCI.15-08-05439.1995
EM Pich
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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M Lorang
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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M Yeganeh
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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F Rodriguez de Fonseca
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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J Raber
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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GF Koob
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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F Weiss
Department of Neuropharmacology, Scripps Research Institute, La Jolla, California 92037, USA.
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Abstract

Previous research has suggested a role for corticotropin-releasing factor (CRF) in the anxiogenic effects of stressful stimuli and ethanol withdrawal. This hypothesis was explored in a series of experiments using intracranial microdialysis to monitor CRF-like immunoreactivity (CRF-IR) in the extracellular compartment of the rat amygdala. The synaptic origin of CRF-IR release in the amygdala was determined in vitro by assessing the Ca2+ dependency of 4-aminopyridine stimulated CRF-IR release from tissue preparations of rat amygdala. In vivo experiments were performed in awake rats after the placement of microdialysis probes in the amygdala. In the first experiment, transient restraint stress (20 min) produced an increase of CRF-IR release (basal levels, 1.19 +/- 0.15 fmol/50 microliters; stress levels, 4.54 +/- 1.33 fmol/50 microliters; p < 0.05) that returned to basal values within 1 hr. When 4-aminopyridine (5 mM) was added to the perfusion medium, it consistently increased CRF-IR release (4.83 +/- 0.92 fmol/50 microliters, p < 0.05). In the second experiment, CRF-IR release was measured during ethanol withdrawal in rats previously maintained for 2–3 weeks on a liquid diet containing ethanol (8.5%). Basal CRF-IR levels were 2.10 +/- 0.43 fmol/50 microliters in ethanol exposed rats and 1.30 +/- 0.19 fmol/50 microliters in control rats. During withdrawal, a progressive increase of CRF-IR levels over time was observed, reaching peak values at 10–12 hr after the onset of withdrawal (10.65 +/- 0.49 fmol/50 microliters vs 1.15 +/- 0.30 fmol/50 microliters of control rats, p < 0.01).

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The Journal of Neuroscience: 15 (8)
Journal of Neuroscience
Vol. 15, Issue 8
1 Aug 1995
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Increase of extracellular corticotropin-releasing factor-like immunoreactivity levels in the amygdala of awake rats during restraint stress and ethanol withdrawal as measured by microdialysis
EM Pich, M Lorang, M Yeganeh, F Rodriguez de Fonseca, J Raber, GF Koob, F Weiss
Journal of Neuroscience 1 August 1995, 15 (8) 5439-5447; DOI: 10.1523/JNEUROSCI.15-08-05439.1995

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Increase of extracellular corticotropin-releasing factor-like immunoreactivity levels in the amygdala of awake rats during restraint stress and ethanol withdrawal as measured by microdialysis
EM Pich, M Lorang, M Yeganeh, F Rodriguez de Fonseca, J Raber, GF Koob, F Weiss
Journal of Neuroscience 1 August 1995, 15 (8) 5439-5447; DOI: 10.1523/JNEUROSCI.15-08-05439.1995
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