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Articles

Presynaptic differentiation induced in cultured neurons by local application of basic fibroblast growth factor

Z Dai and HB Peng
Journal of Neuroscience 1 August 1995, 15 (8) 5466-5475; DOI: https://doi.org/10.1523/JNEUROSCI.15-08-05466.1995
Z Dai
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA.
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HB Peng
Department of Cell Biology and Anatomy, University of North Carolina at Chapel Hill 27599, USA.
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Abstract

Recent studies have suggested a role for molecules residing at the muscle surface in signaling presynaptic development at the neuromuscular junction (NMJ). Since heparan sulfate-proteoglycan is a major component of the extracellular matrix of skeletal muscle, factors that are bound to this proteoglycan, such as basic fibroblast growth factor (bFGF), are in a strategic position for neuronal signaling. To test this idea, we applied bFGF to cultured Xenopus spinal cord neurons and monitored the change in intracellular Ca2+ level with fura-2 ratio imaging. In one-third of the neurons, local application of bFGF elicited a 30–140% increase in intracellular Ca2+ level. Ca(2+)-free medium or suramin abolished this change. Latex beads coated with bFGF induced clustering of synaptic vesicles at the bead-neurite contacts as evidenced by anti-synaptotagmin antibody labeling and electron microscopy. This response was also blocked by Ca(2+)-free medium and by suramin. Uncoated beads or beads coated with PDGF were ineffective. This induction was also inhibited by a tyrosine kinase inhibitor, tyrphostin RG-50864, suggesting the role of receptor tyrosine kinase activation in this process. In addition, bFGF-coated beads also induced the localization of depolarization-dependent Ca2+ influx to the bead- neurite contacts. In contrast, depolarization caused a distributed Ca2+ elevation in untreated neurites. These results suggest that local presentation of bFGF can mimic the muscle target in signaling the development of both a cytoplasmic and a membranous specialization for excitation-secretion coupling observed at the NMJ.

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The Journal of Neuroscience: 15 (8)
Journal of Neuroscience
Vol. 15, Issue 8
1 Aug 1995
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Presynaptic differentiation induced in cultured neurons by local application of basic fibroblast growth factor
Z Dai, HB Peng
Journal of Neuroscience 1 August 1995, 15 (8) 5466-5475; DOI: 10.1523/JNEUROSCI.15-08-05466.1995

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Presynaptic differentiation induced in cultured neurons by local application of basic fibroblast growth factor
Z Dai, HB Peng
Journal of Neuroscience 1 August 1995, 15 (8) 5466-5475; DOI: 10.1523/JNEUROSCI.15-08-05466.1995
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