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Cloning of rat interleukin-3 receptor beta-subunit from cultured microglia and its mRNA expression in vivo

K Appel, M Buttini, A Sauter and PJ Gebicke-Haerter
Journal of Neuroscience 1 August 1995, 15 (8) 5800-5809; DOI: https://doi.org/10.1523/JNEUROSCI.15-08-05800.1995
K Appel
Department of Psychiatry, University of Freiburg Medical School, Germany.
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M Buttini
Department of Psychiatry, University of Freiburg Medical School, Germany.
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A Sauter
Department of Psychiatry, University of Freiburg Medical School, Germany.
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PJ Gebicke-Haerter
Department of Psychiatry, University of Freiburg Medical School, Germany.
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Abstract

The high-affinity receptors for interleukin-3 (IL-3), GM-CSF, and IL-5 are composed of a ligand binding (alpha-) and a transducing (beta-) subunit. Two distinct transducing subunits (clones AIC2A and AIC2B) have been cloned from mouse, whereas in humans, only one (common) beta- subunit (beta c) has been found. A PCR-based cloning strategy was used to obtain a full-length cDNA sequence from rat microglia including 5′- untranslated regions. Sequence analysis revealed a number of features indicative of the presence of only one beta-subunit in the rat. Most likely, the new rIL-3R beta cDNA is the rat equivalent of human respective murine (AIC2B) beta c subunits. Regulation of rIL-3R beta mRNA expression was investigated in cultured microglia and in vivo. Purified microglia expressed significant amounts of rIL-3R beta mRNA. Addition of lipopolysaccharide (LPS) resulted in a marked upregulation of rIL-3R beta mRNA within approximately 4 hr. No downregulation was observed within 1 week's treatment. No rIL-3R beta mRNA was detectable in normal rat brain. However, 3 hr after a single injection of LPS into the tail vein of a rat, a marked induction of receptor mRNA occurred in a variety of brain regions. Transcriptional rates subsided significantly after 24 hr. rIL-3R beta mRNA was visualized by in situ hybridizations with cRNA antisense probes in ramified cells formerly characterized as microglial cells. rIL-3R beta mRNA was also induced in rat brain after occlusion of middle cerebral artery (MCAO).

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The Journal of Neuroscience: 15 (8)
Journal of Neuroscience
Vol. 15, Issue 8
1 Aug 1995
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Cloning of rat interleukin-3 receptor beta-subunit from cultured microglia and its mRNA expression in vivo
K Appel, M Buttini, A Sauter, PJ Gebicke-Haerter
Journal of Neuroscience 1 August 1995, 15 (8) 5800-5809; DOI: 10.1523/JNEUROSCI.15-08-05800.1995

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Cloning of rat interleukin-3 receptor beta-subunit from cultured microglia and its mRNA expression in vivo
K Appel, M Buttini, A Sauter, PJ Gebicke-Haerter
Journal of Neuroscience 1 August 1995, 15 (8) 5800-5809; DOI: 10.1523/JNEUROSCI.15-08-05800.1995
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