Article Figures & Data
Figures
- Fig. 1.
Aberrant neuromuscular morphology in Fas II mutants. A, B, Fluorescence micrographs of NMJs of ventral longitudinal muscles 6 and 7 in mutant (A) and control (B) animals. Arrows point to varicosities of axon 1, and arrowheads point to varicosities of axon 2. Scale bar (shown in A), 10 μm. C, Summary of varicosity counts obtained from 18 mutant and 13 control NMJs from abdominal segment 4. The error bars represent the SEM in this and subsequent figures. Mutant and control animals are from the e76 and e93 P-element excision lines described in Grenningloh et al. (1991).
- Fig. 2.
Synaptic transmission at the whole muscle level is not affected by aberrant NMJ morphology. A, Examples of single traces showing EJCs (A1) and EJPs (A2). Two thresholds of excitation are shown.B, Summary of EJC amplitudes in mutants and controls measured in 1.0 mm calcium from muscle 6 of abdominal segments 4 and 5 from mutant and control animals (n = 15 mutant cells and 9 control cells). There is no significant difference between controls and mutants.
- Fig. 3.
Short-term facilitation. A, Example of frequency-dependent short-term facilitation of the maximal evoked response for mutant and control animals. The traces show synaptic facilitation observed with a 3 pulse train of 20 Hz stimulation recorded in 0.75 mm calcium and represents the average of five individual traces. B, Summary of facilitation ratios (third pulse amplitude/first pulse amplitude × 100) observed from six mutant and nine control cells. There is no significant difference.
- Fig. 4.
Calcium dependency of transmitter release. Maximal EJC amplitude is plotted as a function of external calcium concentration on log–log scales. Each point is the average of data collected from 10 to 14 muscle fibers.
- Fig. 5.
Frequency of spontaneous neurotransmitter release is higher, and amplitude of quantal events is larger, in mutant animals. A, Traces of membrane potential showing spontaneous transmitter release (miniature potentials) in mutants and controls. Calibration bar: 2 mV, 1 sec. The downward deflections in the mutant trace are 1 mV calibration pulses; ∼4 sec of data are shown.B, Summary of frequency of spontaneous miniature potentials from 10 mutant and 8 control cells. C, Amplitude histogram of spontaneous miniature potentials recorded from mutant and control animals. These histograms were constructed from data recorded from four mutant (244 events) and four control (222 events) cells. The data are grouped into 0.3 mV bins.
- Fig. 6.
Mutant varicosities have fewer failures of transmitter release. A, Single traces of focally recorded synaptic current from mutant (left) and control (right) animals. Arrows point to stimulus artifacts, and asterisks indicate events scored as release of transmitter. The events in these traces are unitary quantal events, as judged by their similarity to spontaneously occurring events recorded at the same time. In this recording configuration, the current records represent only a fraction of the total membrane current because of the relatively low seal resistance between the micropipette and the muscle; thus, the scale bars do not represent total membrane current.B, Summary of the mean number of failures of evoked release for mutants (n = 20 sites) and controls (n = 17 sites). The symbols show results obtained from each individual recording site. One hundred stimuli from each site were scored for failure or release.
- Fig. 7.
EJPs recorded with focal calcium application. A micropipette containing 2 mm calcium was placed over several regions of the nerve terminal on each muscle fiber and covered areas of both varicosity types. The segmental nerve was stimulated at a voltage to recruit both axons, and EJPs were recorded with an intracellular electrode. The bathing solution contained 0 calcium. A, Example of raw trace showing evoked EJP (top trace) and synaptic event recorded through the focal pipette (bottom trace). B, The bar graph shows the mean EJP amplitude of data collected from mutant (n = 18) and control (n = 19) sites. The symbolsrepresent the results obtained from individual recording sites.
- Fig. 8.
Nerve terminal ultrastructural of a Fas II mutant. Electron micrographs of mutant (A1) and control (A2) larval NMJ from abdominal segment 4 showing densely staining synapses (arrows), presynaptic dense bodies (arrowheads), subsynaptic reticulum (SR), and muscle fibers (MF). Axons 1 and 2 are labeled Ax1 andAx2, respectively. The scale bar is 0.5 μm and applies to both A1 andA2.
- Fig. 9.
Summary of ultrastructural data. Reconstructed nerve terminals were analyzed for synaptic area (A) and the number of presynaptic dense bodies per synapse (B). The frequency distribution of synapse size is shown for axon 1 (C1) and axon 2 (C2).
Tables
Control Mutant Axon 1 Axon 2 Axon 1 Axon 2 Terminal length sampled (μm) 17.0 20.0 21.3 42.6 Surface area sampled (μm2) 76.8 71.0 109.1 80.8 Number of varicosities sampled 3 5 4 6 Number of synapses analyzed 41 37 44 48 Complete 32 31 30 27 Incomplete 9 6 14 21 Mean synapse size (μm2) 0.31 0.27 0.92 0.66 Total number of active zones 30 36 113 90 Estimated active zones per varicosity 10 7 28 15 Active zones/terminal surface area (#/μm2) 0.4 0.5 1.0 1.1 Active zones/terminal length (#/μm) 1.8 1.8 3.6 2.1 Active zones per synapse 0.7 1.0 2.6 1.9 Number of synapses with: 0 active zones 17 (41%) 13 (35%) 5 (11%) 13 (27%) 1 active zone 20 (49%) 14 (37%) 17 (39%) 10 (21%) 2 active zones 3 (7%) 7 (19%) 6 (14%) 12 (25%) 3 or more active zones 1 (2%) 3 (8%) 16 (36%) 13 (27%) These data are the mean values obtained from two control and three mutant series of sections from abdominal segment 4. Nerve terminal lengths are different for the two axons within a genotype because of branching of the individual axons.
