Fig. 8. Effects of salicylate on the limited value ofVpkCm. Intracellular pressure of the OHC was reduced directly through the patch pipette to collapse the cell.A, The cell was maintained in collapse after the point indicated by the first dotted line. As expected, collapsing the cell caused Cmpk to increase and VpkCm to shift to a limiting negative value (Kakehata and Santos-Sacchi, 1995). At the second dottedline, 10 mm salicylate was applied extracellularly. VpkCm shifted in the positive direction with a decrease in Cmpk. Washout at the third dotted line caused recovery.B, Capacitance function determined by the stair-step protocol for the same cell. Fits (solid lines) to the capacitance data indicate VpkCm,Qmax, and z of −14.3 mV, 4.74 pC, and 0.650 (closed circles, control); −41.0 mV, 4.09 pC, and 0.743 (closed triangles, after collapse); −32.3 mV, 3.86 pC, and 0.418 (open triangles, during application of salicylate); −53.6 mV, 3.54 pC, and 0.728 (closed triangles, after wash). C, Data from five cells showing consistent positive shift ofVpkCm and decrease inCmpk in collapsed cells after 10 mm salicylate treatment.