Article Figures & Data
Figures
- Fig. 1.
TH-immunostained coronal section of the VM of a rat with a right 6-OHDA lesion of the nigrostriatal pathway. Note the complete absence of TH-immunoreactive structures in the substantia nigra (pars compacta and reticulata) and ventral tegmental area. Scale bar, 250 μm.
- Fig. 2.
A, TH-immunostained coronal section of the VM of a rat with a right 6-OHDA lesion of the nigrostriatal pathway and an intranigral dopaminergic graft; B, high-power view of the intranigral graft in A. Numerous TH-positive neurons are present in clusters forming dense networks of fibers penetrating the host striatum. Scale bars: A, 300 μm; B: 100 μm.
- Fig. 3.
A, Coronal section of the dopamine-depleted striatum immunostained for TH. Two graft deposits are present. A halo of TH-immunoreactive fibers penetrating the host striatum can be clearly seen surrounding the graft. B, High-power view of the graft showing numerous TH-positive neurons and dense TH immunostaining surrounding the graft. V, Lateral ventricle. Scale bars: A, 400 μm;B: 100 μm.
- Fig. 4.
A, Representative parasagittal section of a rat implanted with simultaneous intrastriatal and intranigral dopaminergic grafts. Dense TH-immunoreactive areas are seen in the VM and striatum: intranigral graft (curved arrow), intrastriatal graft (straight arrow), and TH-immunoreactive axons growing along the internal capsule (arrowheads). B, High-power view of the intrastriatal transplant. C, High-power view of the intranigral transplant. D, High-power view of TH-immunoreactive axons traveling within the internal capsule.E, Close-up of D showing the direction of TH-positive axons. Scale bars: A, 300 μm;B, 75 μm; C, 75 μm; D, 250 μm; E, 100 μm.
- Fig. 5.
Parasagittal section of an intranigral graft in an animal with double grafts. A, TH-immunoreactive axons can be seen arising from the dense cluster of neurons (curved arrow) in the intranigral graft. B, High-power view of A showing axons growing rostrally along the internal capsule and the MFB. C, Close-up of intranigral graft showing a cluster of TH-positive neurons and fibers growing toward the MFB. Scale bars: A, 250 μm;B, 500 μm; C, 1000 μm.
- Fig. 6.
Dark-field microphotographs of a rat implanted with double grafts. A, Intranigral TH-immunoreactive graft (arrows). A dense bundle of axons can be seen growing toward the internal capsule and the MFB (arrowheads). B, High-power view ofA showing TH-immunoreactive fibers coming from the graft. C, Graft-derived fibers entering the internal capsule. D, Numerous TH-immunoreactive fibers growing along the internal capsule. E, Ipsilateral striatum (S) containing a nigral graft (arrow). The graft is located in the striatal/pallidal border.GP, Pallidum; IC, internal capsule.F, Close-up of the transplant (T). Numerous fibers are seen growing into the pallidum. Scale bars:A, 150 μm; B, 500 μm;C, 500 μm; D, 100 μm;E, 75 μm; F, 100 μm.
- Fig. 7.
Bright-field microphotographs showing the intrastriatal dopaminergic graft of an animal transplanted with double grafts. A, TH-immunoreactive graft (arrow) located in the striatal/pallidal border. A dense halo of TH immunoreactivity surrounds the transplant. B, High-power view of A showing TH-immunoreactive fibers from the intranigral graft growing along the internal capsule and reaching the striatum. C, Close-up of the intrastriatal graft. D, Graft/pallidum border showing TH-immunoreactive fibers transversing the pallidum. E, High-power view of the internal capsule containing numerous TH-immunoreactive fibers. Scale bars: A, 400 μm;B, 200 μm; C, 100 μm;D, 100 μm; E, 100 μm.
- Fig. 8.
Fluorescence microphotographs of representative parasagittal sections of intranigral-grafted neurons retrogradely labeled from an FG injection into the striatum. A, Retrogradely labeled neurons within the intranigral graft (NT). B, Retrogradely labeled neurons within the needle tract (arrows inA). C, High-power view of labeled neurons in the intranigral graft. D, High-power view of labeled neurons in the needle tract. Scale bars: A, 250 μm;B, 500 μm; C, 1000 μm.
- Fig. 9.
Representative sections of TH immunostaining of the brain of a 6-OHDA lesioned rat grafted with a nondopaminergic (cortex) intrastriatal graft and a dopaminergic intranigral graft.A, Coronal section of the striata immunostained for TH. Note the lack of TH immunostaining in the striatum ipsilateral to the lesion and the needle track of the nondopaminergic graft (arrow). B, High-power view of the transplanted striatum. No TH immunoreactivity can be seen surrounding the nondopaminergic transplant. The needle track can be clearly seen containing macrophages that have bound secondary antibody.V, Lateral ventricle. C, Sagittal view of the intranigral graft (NT) showing numerous immunoreactive structures. In contrast to Figure 5, no fibers can be seen entering the internal capsule (IC).D, High-power view of the border between the intranigral graft and internal capsule showing the lack of fiber outgrowth across the border. Scale bars: A, 500 μm; B, 250 μm; C, 200 μm; D, 75 μm.
- Fig. 10.
Rotational behavior of rats bearing unilateral 6-OHDA lesions of the nigrostriatal dopaminergic pathway after amphetamine challenge (5 mg/kg, i.p.) before (stipled bars) and 6 weeks after (cross-hatched bars) micrografting of fetal dopaminergic cells into either the striatum or nigra (single grafts) and micrografting into both the striatum and nigra (double grafts). Each bar represents the mean ± SEM total rotations in 60 min; n = 4–6 in each group; *p < 0.05 compared with pregrafting; **p < 0.05 compared with intrastriatal and intranigral grafts.
Tables
Group n Coordinates Total graft volume Total number of cells AP L V Striatal 4 +1.3 2.1 5.5 and 4.3 6 μl 800,000 +0.6 2.9 5.5 and 4.3 +0.3 3.7 5.5 and 4.3 Nigral 4 −4.8 2.0 8.4 and 8.2 6 μl 800,000 −5.0 2.3 8.3 and 8.1 −5.3 2.6 8.2 and 8.0 Double 6 Same coordinates as for striatal and nigral grafts 6 μl 800,000 Double (control) 4 Same coordinates as for striatal and nigral grafts 6 μl 800,000 Toothbar: −3.3. AP, Anterior-posterior from bregma; L, lateral from bregma; V, ventral from brain surface. In animals receiving double grafts, a total of 3 μl of VM cell suspension (400,000 cells each) was transplanted into both the striatum and the nigra. In the double-graft control animals, nondopaminergic (cortex) cells were transplanted into the striatum, and VM cells were transplanted into the substantia nigra.
