Article Figures & Data
Figures
- Fig. 1.
Predicted amino acid sequence and alternative splicing of PSD-93. A, Alignment of PSD-93 with PSD-95 and HDLG-SAP97 indicates an overall amino acid identity of ∼70%. Identical amino acids are indicated by black boxes, and conserved amino acid changes are shaded by gray boxes.B, The N terminus of PSD-93 is alternatively splice prone. Sequences of the four alternative N-terminal transcripts (5′a–d) are indicated. Black triangles denote the sites at which the sequences diverge from PSD-93. The amino acidnumbers in bold refer to that predicted using 5′b of PSD-93. In-frame starter methionines or stop codons are indicated. C, Two different alternatively spliced inserts occur between amino acids K624 and R625. Thearrow indicates the location at which the two isoform insertions occur. The sequences of the alternative insertions are also shown. D, A schematic model showing the domain structure of PSD-93. Identified sites of putative alternative splicing are indicated. (PSD-93 has been given GenBank accession number U50717.)Figure continues.
- Fig. 2.
Tissue expression and alternative splicing of PSD-93. A, Northern blotting of adult rat tissues indicates that PSD-93 is expressed as an ∼7.5 kb transcript that occurs in brain (Br) but not in kidney (Ki), spleen (Sp), liver (Li), heart (He), or skeletal muscle (Sk). C, Northern blotting of brain regions demonstrates that PSD-93 is present in cerebellum (Cb), cortex (Cx), hippocampus (Hi), and striatum (St) but is absent from brainstem (BS). Note that the band in cerebellum migrates slightly faster than that in other brain regions. ForA and B, a probe common to all of the alternatively spliced forms of PSD-93 was used. D, E, The blot in B was sequentially rehybridized with probes corresponding to two of the alternatively spliced N-terminal regions of PSD-93. C, Probing with 5′a reveals that the regional distribution of this splice variant is similar to that of PSD-93.D, 5′b, however, is selectively absent from cerebellum.B, F, Duplicate samples of mRNA were probed for glyceraldehyde 3-phosphate dehydrogenase to demonstrate loading and integrity of the mRNA for the above blots.
- Fig. 3.
Cellular localization of PSD-93 mRNA in adult brain and E15 embryo. In situ hybridization was used to localize transcripts for PSD-93 (A, C, E, G) or sense control (B, D, F, H). a, In adult brain, PSD-93 seems to be neuron-specific and is highly expressed in Purkinje neurons in the cerebellum (Cb) and also occurs in pyramidal and granule cells in hippocampus (H). b, In E15 embryo, PSD-93 is abundantly expressed in neurons of spinal cord (SC), dorsal root ganglia (DRG), and intestine (In). PSD-93 is also observed in cells of the thymus (Thy) and submandibular gland (SG).
- Fig. 4.
Specificity of antisera to PSD-93 and PSD-95. Western blot analysis reveals that the predominant PSD-93 protein product in rat brain migrates at 103 kDa, whereas the major PSD-95 reactive band migrates at 95 kDa. Crude adult rat brain homogenates (50 μg of protein/lane) were size-fractionated by SDS-PAGE and analyzed by immunoblotting with affinity-purified antiserum to either PSD-93 (lane 1) or PSD-95 (lane 2). Size markers are in kilodaltons.
- Fig. 5.
PSD-93 is postsynaptic, whereas PSD-95 is predominantly presynaptic in rat cerebellum. A sagittal section of rat cerebellum was processed for indirect immunofluorescent double-labeling using a guinea pig antiserum to PSD-93 and a rabbit antiserum to PSD-95. A, PSD-93 immunoreactivity, visualized in thered channel, is present in Purkinje cell somata and molecular layer (M) of cerebellum (100× magnification). B, PSD-95, visualized in thegreen channel, is present in the synaptic plexus of basket cell axons beneath the Purkinje cell layer (P) (100× magnification). C, Higher-power double exposure shows that PSD-93 immunoreactivity (orange) is confined to Purkinje neurons (arrow), whereas PSD-95 immunoreactivity (green) primarily labels the presynaptic basket cell pinceaus (arrowhead) (400× magnification). Note that the orange colorobserved on double exposure is attributable to the longer exposure times required by FITC filters.
- Fig. 6.
The PDZ repeats of PSD-93 interact with nNOS and the tSXV motif of NMDA receptor 2B. Glutathione-Sepharose beads bound to GST or to a PSD-93 protein fragment (amino acids 77–453) fused toGST (G-P93) were incubated with brain extracts. After the beads were washed extensively, retained proteins were eluted with 0.2% SDS and separated by SDS/PAGE. Western blotting indicates that nNOS is selectively retained by G-P93 (lane 3) but not by GST alone (lane 2). Binding assays performed in parallel indicate that an NMDA receptor 2B C-terminal peptide displaces nNOS from G-P93 completely at 10 μm(lanes 4 and 5), substantially at 5 μm (lanes 6 and 7), and negligibly at 0.1 μm (lanes 8 and9). A control peptide had no effect on nNOS binding to G-P93 at 10 μm (lanes 10 and11). Input = 10% of the protein loaded onto the fusion-protein columns.
- Fig. 7.
The 100 amino acid PDZ domain of nNOS is not sufficient to bind to PSD-93. Glutathione-Sepharose beads bound to GST alone or to GST fusion-protein fragments encoding various N-terminal domains of nNOS (G-nNOS) were incubated with brain extracts. After the beads were washed extensively, retained proteins were eluted with 0.2% SDS and separated by SDS/PAGE. Western blotting with PSD-93 antiserum reveals that PSD-93 does not bind to eitherGST alone (lane 2) or toG-nNOS1-100 (lane 3) but is selectively retained by G-nNOS1-150 (lane 4) and G-nNOS1-195 (lane 5). Input = 10% of the protein loaded onto the fusion-protein columns (lane 1).
Tables
Gal4 DNA binding hybrid Gal4 activation hybrid β-Galactosidase units nNOS (amino acids 1–100) PSD-93 (116–421) 1.23 nNOS (40–150) PSD-93 (116–421) 1.64 nNOS (40–195) PSD-93 (116–421) 1.51 nNOS (100–195) PSD-93 (116–421) 1.06 nNOS (1–150) PSD-93 (116–421) 14.7 nNOS (1–195) PSD-93 (116–421) 17.2 Lamin C (66–230) PSD-93 (116–421) 0.430 Yeast Y187 cells were cotransformed with expression vectors encoding various Gal4 DNA binding domain and Gal4 activation domain fusion proteins. Each transformation mixture was plated on synthetic dextrose plates lacking tryptophan and leucine. Interaction was measured by the liquid culture β-galactosidase assay as described (Fields and Song, 1989; Clonetech).
Values are representative of experiments repeated twice with similar results.
