Fig. 10. Detection of PDGF-αR transcripts in the adult mouse CNS by nonradioactive in situ hybridization. PDGF-αR mRNA are widely expressed in neuronal populations, as illustrated for the hippocampus (A), subiculum and entorhinal cortex (B), substantia nigra pars compacta (C), Purkinje cell layer of the cerebellum (D), and neurons of the vestibular nucleus (E). Hybridization signal is not observed in white matter structures, such as the corpus callosum (A, B).F, Control in situ hybridization with the PDGF-αR digoxigenin-labeled oligonucleotide sense probe performed on sagittal brain tissue section through the cerebellum.A–F, Bright-field and phase-contrast.DG, Dentate gyrus; CA1,CA3, hippocampal fields; CC, corpus callosum; ML, molecular layer; PL, Purkinje cell layer; GL, granule cell layer. Magnification: A, 110×; B, D, F, 220×;C, E, 300×.