Article Figures & Data
Figures
- Fig. 1.
A, Effect of electrical stimulation on BK-induced PE in the rat knee joint. In both groups of rats, baseline PE was established in the first three samples. In one group, BK (150 nm; ○, n = 8) was then added to the perfusion fluid (0.9% sodium chloride) and, for the remainder of the experiment, was the only substance in the perfusion fluid. In the second group (•, n = 6), BK was added after the first three baseline samples, and then 40 min after beginning knee perfusion, a noxious electrical stimulation of the hindpaw (ES, 25 mA, 0.5 msec. pulse duration, 1 Hz) was applied continuously for the remainder of the experiment. B, Effect of electrical stimulation on PAF-induced PE in the rat knee joint. In both groups of rats, baseline PE was established in the first three samples. In one group, PAF (35 nm; ○, n = 6) was then added to the perfusion fluid (0.9% sodium chloride) and, for the remainder of the experiment, was the only substance in the perfusion fluid. In the second group (•, n = 8), PAF was added after the first three baseline samples, and then 40 min after beginning knee perfusion, a noxious electrical stimulation (25 mA) was applied to the hindpaw continuously for the remainder of the experiment. In this and subsequent figures, data are presented as mean ± SEM ofn values, and ordinate is absorbance of light at 620 nm. After a drug is added to the perfusate, it is present throughout the experiment.
- Fig. 2.
Effect of sympathectomy on noxious stimulation-induced inhibition of BK-induced PE. For both groups in this figure, BK (150 nm) was added to the perfusion fluid after the first three baseline samples and remained in the perfusion fluid for the duration of the experiment. Both groups had undergone sympathectomy 1 week before the perfusion experiment. The first group (○, n = 15) received BK only, whereas the second group (•, n = 14) also received continuous noxious electrical stimulation of the hindpaw (ES, 25 mA, 0.5 msec, 1 Hz) 40 min after beginning knee perfusion.
- Fig. 3.
A, Effect of intravenous corticosterone on BK-induced PE in the rat knee joint. In both groups of rats, baseline PE was established in the first three samples. In one group, BK (150 nm; ○, n = 8) was then added to the perfusion fluid (0.9% sodium chloride) and, for the remainder of the experiment, was the only substance in the perfusion fluid. In the second group (•, n = 12), BK was added after the first three baseline samples, and then 40 min after knee perfusion corticosterone was infused intravenously at a rate of 5 μg/min for the remainder of the experiment. B, Effect of intravenous corticosterone on PAF-induced PE. For both groups in B, PAF (35 nm) was added to the perfusion fluid after the first three baseline samples and remained in the perfusion fluid for the duration of the experiment. The first group (○, n = 6) were normal animals, and the second group (•, n = 7) received corticosterone (5 μg/min, i.v.) 40 min after beginning knee perfusion.
- Fig. 4.
Effect of sympathectomy on corticosterone-induced inhibition of BK-induced PE. For both groups in this figure, BK (150 nm) was added to the perfusion fluid after the first three baseline samples and remained in the perfusion fluid for the duration of the experiment. Both groups had undergone sympathectomy 1 week before perfusion experiment. The first group (○, n = 15) received BK only, whereas the second group (•, n= 9) also received corticosterone (5 μg/min, i.v.) 40 min after beginning knee perfusion.
- Fig. 5.
Effect of intra-articular phentolamine or ICI 118,551 on corticosterone-induced inhibition of BK-induced PE. For all groups in this figure, bradykinin (150 nm) was added to the perfusion fluid after the first three baseline samples and remained in the perfusion fluid for the duration of the experiment. The first group (○, n = 8) received BK only, whereas the second group (•, n = 5) also received phentolamine (1 μm) co-perfused with BK in the knee joint. The third group (▪, n = 5) received BK with ICI 118,551 (100 nm). Corticosterone infusion (5 μg/min, i.v.) was started 40 min after beginning knee perfusion.
