Fig. 2. Kv1.1 distributions in demyelinated and early remyelinating axons. a, b, A node, at 6 dpi, double-labeled for Kv1.1 (a) and Na+ channels (b) has lost the restriction of Kv1.1 to the juxtaparanode. Instead, the Kv1.1 immunoreactivity has moved into nodal (arrowhead) and paranodal zones, whereas Na+ channels remain very focal. c, d, A node, at 6 dpi, double-labeled for Kv1.1 (c) and Na+ channels (d). Kv1.1 staining is undetectable in many demyelinated axons at this time.e, f, A demyelinated axon, at 7 dpi, double-labeled for Kv1.1 (e) and MAG (f). In e, weak Kv1.1 staining was observed adjacent to an associated cell (*). g,h, A new presumptive node, at 9 dpi, double-labeled for Kv1.1 (g) and MAG (h). Remyelinating Schwann cells had diffuse cytoplasmic Kv1.1 staining, but no immunoreactivity was detected at the presumptive node (arrowhead). Diffuse MAG immunoreactivity indicates early remyelination. Scale bars: a, b,g, h, 25 μm; c,d, e, f, 12 μm.