Article Figures & Data
Figures
- Fig. 1.
Top. Sections of a brain from a sham-operated rat hybridized with 35S-Ptnantisense cRNA probe (A, dark field; B, bright field) or 35S-Ptn sense cRNA probe (C, bright field). Ptn hybridization signal was highly expressed in neurons of cortex (large arrow). A little signal was found in glial cells (small arrow) and microvascular endothelium (medium arrow). D (bright field), Section hybridized with 35S-PTN antisense cRNA shows that PTN mRNA decreases to a greater degree in degenerating neurons in the ischemic core (C) then in the periphery (P) of the infarct compared with normal neurons (N) 6 hr after reperfusions. Magnification:A–C, 200×; D, 100×.
- Fig. 2.
Coronal sections of a brain 3 d after ischemia hybridized with 35S-Ptn antisense cRNA probe (dark field). A strong hybridization signal is shown at the border of the infarct (arrows) and microvasculature in the infarct (B, arrows). Magnification:A, 10×; B, 40×.
- Fig. 3.
Sections of a brain 3 d after ischemia hybridized with 35S-Ptn antisense cRNA (A, B) and immunostaining with anti-PTN antibody (C) or anti-GFAP antibody (D). Ptn hybridization signal was predominantly expressed in the neurons (large arrow) and glial cells (small arrow) along the border of the infarct and blood vessels (arrowhead) immediately adjacent to the infarct (top right in A, dark field, B, bright field). PTN immunoreactivity was detected in glial cells (large arrow) at the border of the infarct (C), which corresponded to the hypertrophic anti-GFAP(+) astrocytes in the similar area on day 3 (D, large arrow); the small arrow denotes neurons under degeneration. Magnification, 200×.
- Fig. 4.
Sections of an ischemic brain on day 3 hybridized with 35S-Ptn antisense cRNA (A, C, dark field; B, D, bright field). PTN transcripts were found in the endothelium of blood vessels (large arrow) and glial cells (small arrow). Endothelial sprouts were seen in Aand B (large arrow). Magnification, 200×.
- Fig. 5.
Sections of an ischemic brain on day 3. A, B, PTN immunoreactivity was found in endothelial cells (small arrow) and macrophages (large arrow) in the infarcted region after staining with anti-PTN antibody. C, D, Macrophages surrounding blood vessel (large arrow) were identified by immunostaining with anti-OX42 antibody (C, arrow, frozen section) and histochemistry staining with GSA-IB4(D, arrow). Magnification: A, B, 400×;C, D, 200×.
- Fig. 6.
Sections of ischemic brain on days 3, 7, and 14.A, 35S-Ptn cRNA hybridization signals were detected in macrophages (arrow) near the infarcted area (top) on day 3 (bright field).B, A number of macrophages with PTN immunoreactivity (small arrow) with variable morphology in the infarcted area on day 7 (large arrow denotes a blood vessel).C, 35S-Ptn antisense cRNA hybridization signals were detected in numerous macrophages (arrow) surrounding residual necrotic tissue at the infarcted region on day 14 (bright field). D, A number of macrophages stained with GSA-IB4 with various morphological features in the infarct on day 14. Magnification:A, B, 400×; C, D, 100×.
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