Fig. 4. Southern blot analysis of the 5-HTap1gene. Genomic DNA was isolated from ovotestis, purified, and digested with BamHI (lane 1), BglII (lane 2), EcoRI (lane 3),HindIII (lane 4),SacI (lane 5), or XbaI (lane 6), as described previously (Wickham and DesGroseillers, 1991). Digested DNA was run on a 1% agarose gel, transferred to a Hybond N+ membrane, and hybridized with a32P-labeled 5-HTap.B cDNA fragment (nt 457–1927).BglII, EcoRI, SacI, andXbaI sites are not present in the probe sequence, whereas BamHI and HindIII appear once. The molecular weight marker is λ phage DNA digested withHindIII. The molecular weights are indicated in kilobase pairs.