Fig. 12. Immunostaining for MYT1(A,C, E, G,J) and MYT1L (B, D,F, H, I,K) proteins in normal and injured spinal cord.A, In the normal spinal cord, MYT1 immunoreactivity is present in cells of the central canal, in large neurons in the gray matter, and to a lesser extent in cells in the white matter.B, MYTL1 immunoreactivity in the uninjured cord is very faint. C, At the lesion epicenter 6 weeks after SCI, the intensity of MYT1 immunostaining is increased both in the peripheral rim of preserved white matter and in the central lesion zone.D, MYT1L staining at the epicenter in an adjacent section is also increased but limited to portions of the peripheral rim of white matter. E, Four millimeters distal to the epicenter, MYT1 expression is still greater than that in normal cord both in large neurons in the gray matter and in the white matter.F, An adjacent section shows increased MYT1L immunoreactivity. G, The most intense MYT1 expression is in the ventromedial white matter (boxed region inE) where many nuclei of small round cells are positive as well as in the cytoplasm of cells with the morphology of astrocytes.H, Enlargement of the boxed region inB shows MYT1L immunoreactivity only in the most superficial layers of the dorsal horn of normal spinal cord.I, Similar enlargement of the dorsal horn (boxed region in F) indicates that after SCI the proportion of MYT1L-labeled cells is much increased.J, Double immunocytochemistry for MYT1 (brown) and GFAP (blue) shows that reactive astrocytes in white matter express both antigens (arrow) in their hypertrophic processes. MYT1-positive nuclei (arrowhead) are also present in many GFAP-negative cells. K, Double immunocytochemistry for MYT1L (brown) and GFAP (blue) shows that some reactive astrocytes in white matter express both antigens (arrow); other astrocytic processes are MYT1L-negative. MYT1L staining is seen in the nuclei of GFAP-negative cells in the white matter (small arrowhead) and in both the nucleus and cytoplasm of large ventral horn neurons (large arrowhead) in the adjacent gray matter. cc, Central canal; dfu, dorsal funiculus; GM, gray matter; lfu, lateral funiculus; vfu, ventral funiculus. A–F: 35×. G–I: 135×. J, K: 320×.