Article Figures & Data
Figures
- Fig. 1.
Nodal and paranodal abnormalities in the CGT-deficient mice. Myelinated processes from the cervical region of the spinal cord of 30-d-old CGT-deficient mice demonstrating ultrastructurally normal (A) and abnormal nodes of Ranvier (B–D). B, An aberrantly formed node with lateral loops of the outermost lamellae forming farther from the node than the more medial lamellae, and these loops are facing away from the axon. Furthermore, the length of the nodes (bracketed) in the CGT-deficient mice is typically longer than in the age-matched wild types (bracketed inA). C, The most commonly observed aberrant nodal structure was the heminode, a myelinated segment adjacent to a nonmyelinated region of the axon. In addition, heminodes are the extreme example of node elongation. D, Less frequently observed was the overlapping of paranodal regions. Two myelin sheaths (1, 2) failed to form a node of Ranvier, because the regions of lateral loop formation overlap, thus excluding the nodal region. Scale bar, 1.0 μm.
- Fig. 2.
High magnification of aberrant paranodal structures in the CGT mutant animals. A, A paranodal region from a 30-d-old littermate control displays a periaxonal space with regularly arrayed densities known as transverse bands (arrowheads). These structures are prominent features of the junctional complex normally formed between the axolemma and the myelin sheath. B, Transverse bands were not found in age-matched CGT-deficient mice. Lateral loops in both the spinal cord (C) and brain (D) frequently are reversed and face away from the axon. Furthermore, the outer most lamella terminates farther from the node. Ax, Axon; G, glial fibrillary acid protein. Scale bar, 0.1 μm.
- Fig. 3.
Astrocytic intrusion between the myelin sheath and the axon. Myelinated process from the spinal cord of a CGT mutant animal demonstrating the intrusion of an astrocytic process, as identified by the presence of glycogen particles (arrowheads) and bundles of filaments (stars), between the myelin sheath and the axolemma. Scale bar, 1.0 μm.
- Fig. 4.
Northern and Western blot analyses of myelin proteins. A, Message levels of MBP and PLP are similar between CGT-deficient mice and littermate controls at 30 and 45 d of age. B, Analysis of both total brain and isolated myelin revealed no difference in the levels of MBP and PLP at 30 and 45 d of age.
- Fig. 5.
Lipid analysis of the PNS. Both NFA- and HFA-containing GalC and sulfatide were absent in the sciatic nerves of the CGT−/− mouse. Bands with mobility patterns consistent with HFA–glucocerebroside and sphingomyelin were prominent in the mutant but not in the wild-type. Chol, Cholesterol;HFA-GalC, hydroxy fatty acid galactocerebroside;NFA-GalC, normal fatty acid galactocerebroside;HFA-GlcC, hydroxyglucocerebroside; PE, phosphatidyl ethanolamine; PC, phosphatidyl choline;SPM, sphingomyelin; HFA-SPM, hydroxy fatty acid sphingomyelin.
- Fig. 6.
Structural analysis of the PNS. Sciatic nerve myelin sheaths revealed no differences between CGT+/+ (A, C) and CGT−/− (B, D) mice with respect to thickness (A, B) or node formation (C, D). Scale bar, 1.0 μm.
- Fig. 7.
Electrophysiological analysis of the PNS.A, Diagram of the recording apparatus used for measurement of conduction velocity. The isolated nerve was placed in a Plexigal chamber and superfused with oxygenated Krebs’ solution, and one end was isolated by a flowing sucrose gap in a separate chamber slowly perfused with isotonic KCl solution (120 mm). Silver–silver electrodes were used to record the potential across the gap and to stimulate the nerve at two points, 5.5 mm apart in the main chamber. An example of compound potentials recorded in response to stimulation of the nerve at proximal and distal electrode pairs is illustrated in B. The conduction time for the length of nerve between the stimulating electrodes was derived from the time between the compound potential peaks in each recording.C, Three recordings are superimposed showing the effect of superfusing a nerve from a CGT+/+ mouse with Krebs’ solution containing 100 μm 4-AP. There was a slight increase in amplitude of the response and the depolarizing afterpotential. The compound action potential increase reversed more readily with 10 min of washing in normal Krebs’ solution. D, Recordings, similar to those in C, show a slightly more pronounced effect of 4-AP on the amplitude of the compound potential and depolarizing afterpotential in a nerve from a CGT−/− mouse.
Tables
- Table 1.
Characteristics of compound action potentials from the sciatic nerve of CGT+/+ and CGT−/− mice
+/+ −/− Amplitude at 37°C 33.6 ± 5.5 mV 15.9 ± 2.9 mV* Conduction speed at 37°C 32.3 ± 2.7 m/sec 22.9 ± 2.2 m/sec* Response to 4-AP (% amplitude increase) 2.0 ± 1.7 3.1 ± 2.7 Compound action potentials were recorded from acutely isolated sciatic nerves from CGT+/+ (n = 3) and CGT−/− (n = 4) animals. Mean values ± SE are reported.
*Significant difference between values (p < 0.05, ANOVA).
