Protein Kinase C Disrupts Cannabinoid Actions by Phosphorylation of the CB1 Cannabinoid Receptor

D. E. Garcia; S. Brown; B. Hille; K. Mackie

doi:10.1523/JNEUROSCI.18-08-02834.1998

Article Information

DOI

https://doi.org/10.1523/JNEUROSCI.18-08-02834.1998

PubMed

9526000

Published By

Society for Neuroscience

History

Received December 8, 1997
Revision received January 22, 1998
Accepted January 22, 1998
First published April 15, 1998.

Version of record published April 15, 1998.

Author Information

D. E. Garcia1,3,
S. Brown2,
B. Hille1, and
K. Mackie1,2

¹Departments of Physiology and Biophysics and
²Anesthesiology, University of Washington, Seattle, Washington 98195, and
³Departamento de Fisiologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, CP 04510 Mexico DF, Mexico

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Author contributions

Disclosures

Received December 8, 1997.
Revision received January 22, 1998.
Accepted January 22, 1998.

This work was supported by the W. M. Keck Foundation, an Alexander von Humboldt Stiftung Fellowship, Universidad Nacional Autonoma de Mexico DGAPA and Consejo Nacional de Ciencia y Tecnologia, and National Institutes of Health Grants NS01588, DA08934, DA00286, and NS08174. We thank A. Perdichezzi and B. Murphy for help with phosphorylation assays, D. Anderson and L. Miller for technical help, W. Catterall, H. Cruzblanca, J. Isaacson, E. Kaftan, K.T. Kim, D.-S. Koh, J. Roche, and M. Shapiro for reading this manuscript, Y. Lai and A. Nairn for providing protein kinase C, and Sterling Winthrop for providing WIN 55,212-2.
Correspondence should be addressed to Dr. Ken Mackie, University of Washington, Department of Anesthesiology, Box 356540, Seattle, WA 98195-6540.

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	Abstract	Full	Pdf
Jan 1999	4	5	2
Feb 1999	3	3	3
Mar 1999	2	13	3
Dec 1999	2	5	5
Total 1999	11	26	13
Jan 2000	3	11	3
Feb 2000	4	11	9
Mar 2000	3	14	12
Apr 2000	3	20	15
May 2000	5	9	14
Jun 2000	0	7	7
Jul 2000	2	7	6
Aug 2000	3	8	8
Sep 2000	2	16	5
Oct 2000	4	16	8
Nov 2000	0	8	8
Dec 2000	2	10	7
Total 2000	31	137	102
Jan 2001	0	18	13
Feb 2001	1	16	11
Mar 2001	2	18	11
Apr 2001	2	11	6
May 2001	3	20	14
Jun 2001	4	20	14
Jul 2001	2	8	5
Aug 2001	2	9	4
Sep 2001	2	11	7
Oct 2001	1	11	6
Nov 2001	1	19	14
Dec 2001	1	4	4
Total 2001	21	165	109
Jan 2002	2	21	11
Feb 2002	0	25	11
Mar 2002	3	18	11
Apr 2002	3	25	30
May 2002	3	17	16
Jun 2002	0	15	14
Jul 2002	3	11	14
Aug 2002	0	10	11
Sep 2002	1	11	12
Oct 2002	3	15	13
Nov 2002	2	15	12
Dec 2002	4	7	5
Total 2002	24	190	160
Jan 2003	2	15	17
Feb 2003	0	13	17
Mar 2003	3	13	24
Apr 2003	1	23	18
May 2003	1	13	17
Jun 2003	0	8	10
Jul 2003	12	20	15
Aug 2003	7	20	18
Sep 2003	7	25	16
Oct 2003	7	21	19
Nov 2003	11	16	9
Dec 2003	8	21	7
Total 2003	59	208	187
Jan 2004	12	24	11
Feb 2004	8	19	21
Mar 2004	12	20	11
Apr 2004	15	17	16
May 2004	14	15	14
Jun 2004	12	15	6
Jul 2004	17	16	10
Aug 2004	20	16	7
Sep 2004	15	27	16
Oct 2004	11	28	18
Nov 2004	7	27	14
Dec 2004	9	15	7
Total 2004	152	239	151
Jan 2005	8	24	28
Feb 2005	13	24	28
Mar 2005	22	17	19
Apr 2005	5	18	21
May 2005	5	16	8
Jun 2005	6	15	27
Jul 2005	5	16	8
Aug 2005	9	20	10
Sep 2005	2	16	14
Oct 2005	7	24	6
Nov 2005	9	21	16
Dec 2005	9	19	17
Total 2005	100	230	202
Jan 2006	5	12	8
Feb 2006	9	13	12
Mar 2006	7	17	17
Apr 2006	4	16	9
May 2006	4	19	9
Jun 2006	3	18	13
Jul 2006	6	10	9
Aug 2006	3	14	11
Sep 2006	7	28	15
Oct 2006	2	18	8
Nov 2006	3	11	8
Dec 2006	2	9	6
Total 2006	55	185	125
Jan 2007	2	22	17
Feb 2007	5	22	9
Mar 2007	7	9	11
Apr 2007	10	17	19
May 2007	5	11	8
Jun 2007	3	10	5
Jul 2007	1	14	6
Aug 2007	5	6	5
Sep 2007	3	16	11
Oct 2007	4	10	10
Nov 2007	4	16	16
Dec 2007	2	7	8
Total 2007	51	160	125
Jan 2008	1	8	5
Feb 2008	6	10	9
Mar 2008	6	6	6
Apr 2008	7	17	16
May 2008	11	11	14
Jun 2008	10	15	9
Jul 2008	2	9	9
Aug 2008	6	6	8
Sep 2008	8	10	4
Oct 2008	12	14	16
Nov 2008	10	10	10
Dec 2008	5	8	8
Total 2008	84	124	114
Jan 2009	6	16	15
Feb 2009	3	11	12
Mar 2009	3	16	22
Apr 2009	2	4	11
May 2009	1	4	9
Jun 2009	2	7	6
Jul 2009	1	3	3
Aug 2009	1	4	3
Sep 2009	3	3	8
Oct 2009	2	7	9
Nov 2009	7	12	13
Dec 2009	10	2	6
Total 2009	41	89	117
Jan 2010	6	10	9
Feb 2010	6	3	3
Mar 2010	6	8	7
Apr 2010	2	3	4
May 2010	5	13	12
Jun 2010	9	3	8
Jul 2010	7	16	13
Aug 2010	14	15	12
Sep 2010	6	4	5
Oct 2010	8	9	5
Nov 2010	6	10	4
Dec 2010	5	10	8
Total 2010	80	104	90
Jan 2011	7	7	6
Feb 2011	4	5	12
Mar 2011	10	9	11
Apr 2011	7	6	10
May 2011	10	6	5
Jun 2011	2	7	8
Jul 2011	5	11	14
Aug 2011	3	4	9
Sep 2011	11	6	8
Oct 2011	2	13	8
Nov 2011	8	10	14
Dec 2011	3	12	6
Total 2011	72	96	111
Jan 2012	13	11	19
Feb 2012	6	6	6
Mar 2012	8	1	6
Apr 2012	6	11	23
May 2012	6	3	6
Jun 2012	14	8	10
Jul 2012	9	5	6
Aug 2012	11	13	15
Sep 2012	9	5	13
Oct 2012	9	4	7
Nov 2012	17	3	13
Dec 2012	20	8	6
Total 2012	128	78	130
Jan 2013	9	2	9
Feb 2013	14	7	7
Mar 2013	10	2	4
Apr 2013	14	2	8
May 2013	9	2	5
Jun 2013	7	1	3
Jul 2013	5	6	11
Aug 2013	3	5	9
Sep 2013	4	7	5
Oct 2013	9	6	10
Nov 2013	7	7	4
Dec 2013	8	5	10
Total 2013	99	52	85
Jan 2014	13	13	14
Feb 2014	10	3	4
Mar 2014	5	5	9
Apr 2014	7	4	9
May 2014	17	6	11
Jun 2014	23	5	4
Jul 2014	12	6	9
Aug 2014	10	7	7
Sep 2014	1	8	17
Oct 2014	6	6	12
Nov 2014	2	6	7
Dec 2014	2	4	6
Total 2014	108	73	109
Jan 2015	6	9	10
Feb 2015	2	7	9
Mar 2015	5	8	10
Apr 2015	1	7	10
May 2015	4	6	6
Jun 2015	9	9	6
Jul 2015	4	10	5
Aug 2015	2	4	11
Sep 2015	11	4	4
Oct 2015	1	15	6
Nov 2015	2	10	2
Dec 2015	4	15	6
Total 2015	51	104	85
Jan 2016	3	11	8
Feb 2016	14	16	9
Mar 2016	11	28	14
Apr 2016	9	19	7
May 2016	9	18	4
Jun 2016	4	12	9
Jul 2016	6	23	7
Aug 2016	7	22	11
Sep 2016	1	6	6
Oct 2016	3	3	5
Nov 2016	1	12	3
Dec 2016	0	5	7
Total 2016	68	175	90
Jan 2017	1	10	6
Feb 2017	0	19	11
Mar 2017	1	22	6
Apr 2017	1	8	5
May 2017	1	31	3
Jun 2017	1	4	3
Jul 2017	2	16	11
Aug 2017	1	7	10
Sep 2017	0	5	6
Oct 2017	1	9	5
Nov 2017	0	13	6
Dec 2017	0	6	2
Total 2017	9	150	74
Jan 2018	0	10	3
Feb 2018	1	14	13
Mar 2018	1	12	4
Apr 2018	0	17	2
May 2018	1	26	4
Jun 2018	0	18	2
Jul 2018	1	19	6
Aug 2018	0	28	2
Sep 2018	0	32	3
Oct 2018	2	29	8
Nov 2018	3	39	3
Dec 2018	0	27	6
Total 2018	9	271	56
Jan 2019	0	25	8
Feb 2019	1	20	11
Mar 2019	0	13	8
Apr 2019	4	23	15
May 2019	3	12	14
Jun 2019	1	8	12
Jul 2019	2	13	15
Aug 2019	2	9	11
Sep 2019	3	11	5
Oct 2019	3	12	3
Nov 2019	0	11	12
Dec 2019	3	11	8
Total 2019	22	168	122
Jan 2020	3	17	5
Feb 2020	1	9	6
Mar 2020	2	12	5
Apr 2020	1	12	8
May 2020	4	6	7
Jun 2020	3	10	2
Jul 2020	2	12	5
Aug 2020	1	5	3
Sep 2020	4	32	13
Oct 2020	5	25	5
Nov 2020	3	22	7
Dec 2020	0	18	1
Total 2020	29	180	67
Jan 2021	0	30	9
Feb 2021	2	16	13
Mar 2021	1	14	8
Apr 2021	6	26	8
May 2021	4	15	8
Jun 2021	2	32	9
Jul 2021	2	22	16
Aug 2021	6	31	7
Sep 2021	2	28	7
Oct 2021	2	31	24
Nov 2021	0	15	13
Total 2021	27	260	122
Total	1331	3464	2546