Abstract
Locomotor activity is a polygenic trait that varies widely among inbred strains of mice (Flint et al., 1995). To characterize the role of D2 dopamine receptors in locomotion, we generated F2hybrid (129/Sv × C57BL/6) D2 dopamine receptor (D2R)-deficient mice by gene targeting and investigated the contribution of genetic background to open-field activity and rotarod performance. Horizontal activity of D2R−/− mice was approximately half that of drug-naive, strain-matched controls but was significantly greater than haloperidol-treated controls, which were markedly hypokinetic. Wild-type 129/SvEv and C57BL/6 mice with functional D2 receptors had greater interstrain differences in spontaneous activity than those among the F2 hybrid mutants. Incipient congenic strains of D2R-deficient mice demonstrated an orderly gene dosage reduction in locomotion superimposed on both extremes of parental background locomotor activity. In contrast, F2 hybrid D2R−/− mice had impaired motor coordination on the rotarod that was corrected in the congenic C57BL/6 background. Wild-type 129/SvEv mice had the poorest rotarod ability of all groups tested, suggesting that linked substrain 129 alleles, not the absence of D2 receptors per se, were largely responsible for the reduced function of the F2hybrid D2R−/− and D2R+/− mice. Neurochemical and pharmacological studies revealed unexpectedly normal tissue striatal monoamine levels and no evidence for supersensitive D1, D3, or D4 dopamine receptors in the D2R−/− mice. However, after acute monoamine depletion, akinetic D2R+/− mice had a significantly greater synergistic restoration of locomotion in response to SKF38393 and quinpirole compared with any group of D2R+/+ controls. We conclude that D2R-deficient mice are not a model of Parkinson’s disease. Our studies highlight the interaction of multiple genetic factors in the analysis of complex behaviors in gene knock-out mice.
- D2 dopamine receptor
- genetics
- locomotor activity
- C57BL/6 mice
- 129/SvEv mice
- dopamine
- rotarod
- gene knock-out mice
Locomotor activity requires the coordinated actions of cortical and subcortical structures (for review, see Hikosaka 1991; Graybiel 1991; Mink and Thatch, 1993). The basal ganglia, composed of corpus striatum, globus pallidus, subthalamic nucleus, and substantia nigra (SN), play an important role in modulating the final output of cortical motor neurons. The SN pars compacta is the major source of ascending dopaminergic input to the dorsal striatum. Striatal GABAergic neurons express dopamine receptors that belong to either the family of D1-like receptors (D1 and D1B) linked positively to adenylyl cyclase or to the D2-like receptors (D2, D3, and D4) that are inhibitory to adenylyl cyclase or coupled to G-protein-activated ion channels (Civelli et al., 1993; Gingrich and Caron; 1993, Sibley et al., 1993). Although in situhybridization studies indicated that largely segregated populations of medium spiny neurons express the D1 and D2 receptors (Graybiel, 1991;Le Moine and Bloch, 1995), more recent work using mRNA amplified from single cells demonstrated a high degree of overlap between D1- and D2-like receptors (Surmeier et al., 1996).
Despite the differences in signal transduction mechanisms, the contribution of D1- and D2-like receptors to locomotion through the direct and indirect striatopallidal projections is generally considered synergistic. Hypokinesia or akinesia can be induced pharmacologically in the mouse by antagonists of either the D1-like (Rubinstein et al., 1988; Cabib et al., 1991) or D2-like receptors (Fujiwara, 1992). However, none of the available drugs has complete specificity for any of the five known dopamine receptor subtypes. Administration of D2 antisense oligodeoxynucleotides to rats (Zhang and Creese, 1993) or mice (Zhou et al., 1994) caused only a partial reduction in striatal D2 receptors but reduced spontaneous locomotor activity. Acute monoamine depletion studies have demonstrated that stimulation of both D1- and D2-like receptors is necessary for significant reversal of the resulting akinesia (Jackson and Hashizume, 1986; Starr et al., 1987;Rubinstein et al., 1988; Zarrindast and Eliassi 1991; Ferré et al., 1994). In contrast, selective functional loss of the dopamine D1 receptor by gene targeting was reported to cause either an increase in baseline activity (Xu et al., 1994) or no alteration in locomotion (Drago et al., 1994). Much less is known about the contribution of the remaining dopamine receptor subtypes to locomotion or their capacity to compensate functionally for the more abundant D1 and D2 receptors, although gene deletion studies (Accili et al., 1996; Xu et al., 1997) and experiments using D3-preferring antagonists (Svensson et al., 1994) indicated that the D3 subtype may be predominantly inhibitory. Our laboratory also inferred a predominantly inhibitory role for the D4 subtype on spontaneous locomotion from a gene deletion study (Rubinstein et al., 1997).
To investigate the selective physiological role of the D2 dopamine receptor in locomotor activity, we have produced mice that lack functional D2 receptors by gene targeting in embryonic stem cells (Kelly et al., 1997). The following questions were addressed in the present study. What are the specific components of locomotion that are altered by the absence of D2 receptors? What is the contribution of gene dosage at the D2 receptor locus to locomotion? What is the interaction of the null mutation at the D2 gene locus and genetic background on locomotion? Are there identifiable adaptations in other molecular components of the nigrostriatal dopaminergic circuits in mice lacking D2 receptors? Our results from a combination of behavioral, neurochemical, and pharmacological assays emphasize the critical contribution of genetic background to analysis of a complex phenotype in the gene-targeted mice.
MATERIALS AND METHODS
D2 receptor-deficient mouse strains
The original F2 hybrid strain (129/Sv × C57BL/6J) containing the mutated D2 receptor allele was generated in our laboratory as described previously (Kelly et al., 1997). To establish an incipient congenic B6 strain, D2 dopamine receptor (D2R)+/− mice were backcrossed to wild-type C57BL/6J for five generations. The sex of the +/− mice was alternated between male and female for each successive generation. After the fifth generation backcross, the colony was expanded by inbreeding pairs of nonsibling +/− male and female mice (all parents were N5, making all pups N5 equivalents), and each mouse was genotyped by Southern blot as described (Kelly et al., 1997). The 129/Sv strain mice that the D3 embryonic stem (ES) cells (Doetschman et al., 1985) were derived from are no longer commercially available. Therefore, to establish a substrain 129 line of mutant mice, the original male chimeras derived from the gene-targeted ES cells were bred to closely related wild-type 129/SvEvTac females (Simpson et al., 1997) to produce substrain 129+/− mice. The colony was then expanded as above. For simplicity these lines of mutant mice are referred to as congenic B6 and congenic 129 in this report. Wild-type C57BL/6J mice were obtained from The Jackson Laboratory (Bar Harbor, ME), and 129/SvEvTac mice were obtained from Taconic (Germantown, NY). Mice were fed on the floors of the cages to assure easy access to food. Approximately equal numbers of mice of both sexes were used for all experiments, and the data collapsed across gender if the statistical analyses revealed no gender interaction within a particular measurement.
Assessment of postnatal development
F2 hybrid +/− mice were bred, and the resultant pups were assessed from birth through postnatal day 20 when they were weaned. Physical measurements were recorded daily, including weight, eye opening, and pinnae opening. The righting reflex was assessed by placing each pup on its back and timing the return to the upright position. The ages at which each pup was first observed to perform a variety of locomotor skills, including crawling, walking, rearing, and running, were also recorded. Video recordings of these same mice both as young pups and as adults were made.
Neurochemical assays
D1 receptor saturation binding was performed on striatal membranes (30–50 μg of protein) using the D1-selective antagonist [3H]SCH23390 (specific activity, 81.4 Ci/mmol; DuPont NEN, Boston, MA) as a radioligand (Zhou et al., 1990) by methods described previously (Bunzow et al., 1995; Zhang et al., 1996). The saturation data were analyzed through a nonlinear regression fit of a hyperbolic equation using Graph Pad (San Diego, CA) InPlot software.
D1 receptor autoradiography was performed on 15 μm cryostat brain sections using 1 nm [3H]SCH23390 in the presence of 100 nm ketanserin to block 5-HT2 receptor binding, as described in previous reports (Mansour et al., 1990; Janowsky et al., 1992; LaHoste and Marshall, 1992). Nonspecific binding was demonstrated on a separate set of slides by the addition of 5 μm (+)-butaclamol, a nonselective dopamine receptor ligand.
Monoamines and their metabolites were determined using the methods described previously by Hall et al. (1989). All tissue levels of these compounds were determined as nanograms per gram wet weight of tissue.
Measurement of locomotor activity
Omnitech Digiscan (Columbus, OH) model CCDIGI activity monitors were used to quantify horizontal activity and rearing. All experiments were performed between 8:00 A.M. and 4:30 P.M. using animals maintained on a 12 hr light/dark cycle from 6:00 A.M. to 6:00 P.M. The 40 × 40 cm open-field chambers were enclosed in soundproof boxes that were in turn enclosed in a quiet room separated from the colony area. Horizontal distance was measured by the sequential breaking of infrared beams, 5 cm on center, in the horizontal plane of the x- andy-axes. Initiation of movement was incremented each time a break in ambulatory activity occurred for >1 sec. Movement time was incremented when a mouse was active for >1 sec. The average speed of movement was obtained by dividing total horizontal distance by total time in motion for each mouse in the 30 min testing period. Rearing movements were counted each time an animal passed above and then below the level of a sensor in the z-axis vertical plane (the mouse must have remained below the level of a sensor for at least 1 sec before it could score again).
Pharmacological experiments
Experiment 1. Mice were injected with either saline or 0.3 or 0.6 mg/kg haloperidol (McNeil Pharmaceutical Co., Fort Washington, PA), placed in a holding cage for 15 min, and then tested in the open-field apparatus for 30 min. All drug treatments in experiments 1 and 2 were administered intraperitoneally in a volume of 10 ml/kg.
Experiment 2. Drug-naive mice were pretested in an open-field chamber for 15 min the day before the experiment to habituate them to the apparatus. The day of the experiment, mice were retested for 30 min to ascertain their pretreatment activity levels. All animals were then depleted of monoamines by treatment with 200 mg/kg α-methyl-(dl)-p-tyrosine methyl ester (AMPT) (Sigma, St. Louis, MO) followed 1 hr later by 5 mg/kg reserpine (Sigma). One hour after reserpine treatment animals received a second lower dose of 100 mg/kg AMPT. Similar regimens have been documented to precipitously drop dopamine, norepinephrine, and to a lesser extent, 5-HT levels (Starr et al., 1987). A total of 3 hr after the first AMPT dose, animals were tested in the open field for 30 min to determine their monoamine-depleted activity levels. Mice were then divided within genotype into three groups: saline, 6 mg/kg SKF38393, or 12 mg/kg SKF38393 (Research Biochemicals, Natick, MA) and received this dose immediately before being placed in the open field for 1 hr. After this 1 hr drug-treated trial, each group was retreated with its original dosage of SKF38393 and the addition of 2 mg/kg quinpirole (Research Biochemicals), except the saline group, which again received saline. The animals were immediately returned to the open field for a final 1 hr monitoring period and then killed by inhalation of CO2. Four mice were dropped from the study (depletion failures) because they scored >20% of their premonoamine depletion distance and were >2 SD from the mean percentage scored by their respective group. These four mice consisted of one each F2+/−, F2−/−, and wild-type parental strains.
Rotarod test
Animals were placed in a neutral position on a stationary 6-cm-diameter cylinder. After 3 sec the rotarod was switched on to a speed of 4 rpm, and the mice were timed until they fell from the rotarod (or a maximum cutoff time of 120 sec). Mice that attained a score of 120 sec were removed from the rotarod and returned to their home cages; mice that fell were restarted for a total of three consecutive trials per day for 4 d. Mice that could not remain on the stationary cylinder for three trials scored zero. The highest of the individual scores of each animal on each day were used for analysis.
RESULTS
D2 receptor-deficient mice achieved age-appropriate developmental milestones
All three genotypes of mice on the F2 hybrid background grew normally from birth, with no differences in birth weights and postnatal development, assessed by the timing of eye opening or pinnae opening. Additionally, there were no differences among the three genotypes in their age of acquisition of basic motor skills including the righting reflex, walking, running, rearing, and grasping before weaning (data not shown). The mice had no tremor or ataxia and did not display an abnormal stance or posture when assessed directly in an open-field test or when the same test was reviewed on videotape by observers blind to genotype (data not shown).
D2 receptor-deficient mice had decreased initiation of spontaneous movement
Preliminary experiments demonstrated that both adult F2−/− mice and wild-type 129/SvEv mice displayed impaired motor function relative to wild-type C57BL/6 mice. Consequently, we performed a comprehensive analysis of spontaneous locomotor activity in an open-field on groups of drug-naive mice representing both parental strains as well as the three D2 receptor genotypes of F2hybrid (129/Sv × C57BL/6) mice. Independent measures included total horizontal distance traveled, number of initiations of movement, total time spent in motion, and number of rearing events. The mice ranked in order for total horizontal distance from high to low: C57BL/6, F2+/+, F2+/−, F2−/−, and 129/SvEv (Fig. 1A). The individual scores for each mouse were plotted on a frequency histogram and showed five discreet populations with normal distributions and a nearly complete lack of overlap between the parental strains (Fig. 1B). Within each group of mice there was a similar temporal pattern of habituation to the novel environment of the open-field as demonstrated by higher activity counts in the first time blocks compared with the later blocks (Fig.1C). A statistical analysis demonstrated significant differences by genotype or parental strain in several measures. ANOVA (F(4,175) > 34; p < 0.0001) followed by Tukey post hoc tests revealed that horizontal distance, initiation of movement, time in motion (data not shown), and number of rearing events (data not shown) were lower in F2−/− mice and wild-type 129/SvEv mice compared with F2+/+ and wild-type C57BL/6 mice (p< 0.0001 for each comparison). F2+/− mice also had significantly lower scores in these measures than F2+/+ or wild-type C57BL/6 mice (p, <0.03–0.0001). Only wild-type C57BL/6 mice had greater speed of movement than the other groups (data not shown). The differences in horizontal distance traveled could be attributed mostly to the differences in the absolute number of movement epochs initiated by each genotype or strain (Fig.1A). An ANOVA limited to the wild-type 129/SvEv and F2−/− mice demonstrated that the F2−/− mice actually had significantly higher scores for horizontal distance (F(1,70) = 4.75; p = 0.03), time spent in motion (F(1,70) = 5.47;p = 0.02), and rearing (F(1,70)= 4.39; p = 0.04), but not initiation of movement.
Because there were greater differences in every measure of locomotor activity between the two wild-type parental strains than among the F2 mice with varying numbers of D2 receptors, we produced congenic 129 and B6 strains of D2 receptor-deficient mice to better assess the contribution of the single gene product to a polygenic complex behavior. Locomotor activity measurements for the three D2 receptor genotypes on both congenic backgrounds are shown in Figure2. Because of the passage of time and seasonal differences in animal activity levels a direct comparison with the data in Figure 1 is not appropriate; however, the overall rank orders for total horizontal distance, rearing, and initiation of movement were clearly unchanged. The B6 congenic −/− mice had significantly lower scores for total horizontal distance (p < 0.0001; ANOVA followed by Tukey post hoc tests), initiation of movement (p < 0.0001), rearing (p < 0.0001), and duration of horizontal movements (p < 0.0002) compared with their +/+ siblings. The B6 congenic +/− mice also demonstrated reduced total horizontal distance (p < 0.002) and rearing (p < 0.001) compared with their +/+ siblings.
There were significant deficits in total horizontal distance (p < 0.03; ANOVA followed by Tukey post hoc tests) and initiation of movement (p < 0.0013) in 129 congenic −/− mice compared with 129 congenic +/+ siblings, whereas the +/− mice had a significant decrease only in total horizontal distance (p < 0.05) when monitored in a novel open-field environment. No significant differences were found in the number of rears of the three sibling groups. For both sets of congenic mice, like the F2 mice, the D2 receptor genotype differences in total horizontal distance traveled can be attributed primarily to differences in the initiation of movement and not duration or speed of movement (data not shown).
Locomotion in D2 receptor-deficient mice was not affected by haloperidol
To further assess the involvement of the D2 receptor and contribution of genetic background to the multiple components of locomotor activity, mice of all five groups were treated acutely with 0.6 mg/kg haloperidol, a D2-like receptor antagonist. F2+/−, F2+/+, wild-type 129/SvEv, and wild-type C57BL/6 mice demonstrated a drug-dependent decrease in many locomotor parameters (Table 1). In marked contrast to the other four groups, F2 D2R−/− mice treated with haloperidol were unchanged in all measured parameters. Total horizontal distance scores for all other groups of mice treated with haloperidol were actually significantly lower than the scores of D2R−/− mice (p < 0.0007; ANOVA followed by Tukey post hoc tests). A 0.3 mg/kg haloperidol treatment produced quantitatively smaller changes in all groups and also had no effect on the F2−/− mice (data not shown).
The dose-dependent effects of haloperidol on locomotion in 129/SvEv mice suggest that the low spontaneous activity in this strain is not attributable to a coincidental absence of functional D2 dopamine receptors. 129/SvEv mice also had significant increases in serum prolactin secreted from the anterior pituitary gland in response to haloperidol (data not shown) and had similar D2 binding sites on striatal membranes compared with wild-type C57BL/6 mice measured by saturation ligand binding to [3H]nemonapride as described previously (Kelly et al., 1997). TheBmax was 347 ± 19 or 303 ± 5 fmol/mg protein, and the Kd was 44 ± 11 or 37 ± 9 nm in 129/SvEv and C57BL/6 mice, respectively.
Functional D2 receptors did not correlate with rotarod performance
The ability to balance and walk on a rotating cylinder is a test of coordinated motor skills. Both F2−/− mice and wild-type 129/SvEv mice performed very poorly on the rotarod test with little improvement over the four consecutive days of trials (Fig.3A). ANOVA of the mean scores by genotype on day 4 demonstrated that each of these two groups differed significantly (p < 0.05, Tukeypost hoc analysis) from F2+/−, F2+/+, and wild-type C57BL/6 mice. However, a frequency histogram of the individual maximum scores revealed that the F2+/− mice actually segregated into two distinct performance level populations (Fig. 3B): low performers similar to wild-type 129/SvEv mice and high performers similar to wild-type C57BL/6 mice. Rotarod score distributions of the other four groups of mice were all consistent with single populations.
Because it was difficult to reconcile the unexpected finding of a bimodal distribution of rotarod scores in the F2+/− mice with the unimodal distributions of spontaneous locomotor scores and the 50% reduction in D2 receptor sites compared with F2+/+ mice determined by saturation ligand binding (Kelly et al., 1997) (data not shown) in the same group, we postulated that other polymorphic loci between the substrain 129 and C57BL/6 parental strains of the F2 hybrid mice might explain these data. Therefore we repeated the rotarod study using congenic B6 mice carrying the mutated D2 receptor alleles. Congenic 129 mice were not tested because of the floor effect already present in the wild-type 129/SvEv strain on this test. The rotarod performance over 4 d of trials among the three genotypes of congenic B6 mice differed markedly from the F2hybrid mice (Fig. 3C). All the congenic B6 mice had similar low scores on the first day of trials. On the second and third days the −/− mice scored significantly lower (p < 0.0001; ANOVA followed by Tukey post hoc tests) than both +/+ and +/− siblings. The −/− mice, assessed within subjects, had improved on days 2 and 3, and by day 4 they attained final performance scores indistinguishable from both +/+ and +/− congenic B6 siblings. Although the B6 congenic −/− mice learned how to avoid falling from the rotarod with repeated trials, it was apparent from direct observation that they were never as facile at the task as their siblings with functional D2 receptors. Their qualitative behavior was also very different from that of wild-type 129/SvEv mice, which seemingly made no attempts at all of walking forward when the cylinder began to rotate.
Adaptations of the nigrostriatal dopaminergic pathway in D2 receptor-deficient mice
Adult mutant mice lacking D2 dopamine receptors throughout development consistently maintained ∼50% of the spontaneous locomotor activity of their strain-matched control siblings, moved with the same speed and duration as +/+ mice, and were significantly more mobile than +/+ mice treated acutely with a relatively D2R-selective dose of haloperidol. This degree of locomotor impairment was not obvious on casual observation of the D2R−/− mice and did not impose a disability on the homozygous mutant mice in their controlled laboratory environment. Therefore we hypothesized that some compensatory mechanism was contributing to their relative normalcy.
One possibility was an increased production or turnover of dopamine in the striatum and SN as a result of the loss of D2 autoreceptors. The tissue content of dopamine and its metabolites, serotonin, 5-hydroxyindole-acetaldehyde, and norepinephrine were measured in the dorsal striatum (Table 2) and SN (data not shown) from congenic B6 mice of all three genotypes. Strikingly, no significant differences were found in any of these measurements in either anatomic location among genotypes.
We also characterized D1 receptor binding-sites in dorsal striatum from the mutant mice by saturation ligand binding (Fig.4). These data demonstrated that there was a significant 20% decrease in Bmax[273 ± 5 vs 345 ± 15 fmol/mg protein (F(2,12) = 4.89; p < 0.05)], but no change in Kd (0.27 ± 0.05 vs 0.32 ± 0.05 nm) between −/− and +/+ mice, respectively. Autoradiography using [3H]SCH23390 revealed no qualitative, regional differences in the distribution of D1 binding sites in the brains of D2−/− mice (Fig.5), including ventral striatum and frontal cortex.
To determine whether the locomotor compensation present in adult D2 receptor-deficient mice involved a functional supersensitivity of D1-like receptors, changes in the D3 or D4 receptors, or pathways using monoamines other than dopamine, we performed the following experiment.
Reserpine and AMPT treatment produced akinesia in all mice
Catecholamines and other monoamines were depleted from presynaptic terminals using a combination of reserpine (a monoamine secretory vesicle-depleting agent) (Hornykiewicz, 1966; Fujimiya et al., 1994) and AMPT (a selective inhibitor of tyrosine hydroxylase activity) (Rubinstein et al., 1988). All five groups of mice (wild-type 129/SvEv, F2−/−, F2+/−, F2+/+, and wild-type C57BL/6) displayed significant decreases (F(1,171) > 33; p < 0.0001) within subjects in horizontal distance traveled compared with their pretreatment levels (Fig. 6). An ANOVA showed that the monoamine-depleted horizontal distance scores for 129/SvEv, F2−/−, F2+/−, F2+/+ mice were significantly different from each other (F(4,170) = 2.82; p = 0.03). However, F2−/− mice were only slightly more active than the C57BL/6 mice (p < 0.05 by Tukey post hoc test). These data suggest that functional monoaminergic pathways are essential for the D2 receptor-deficient mice to maintain their basal level of locomotor activity.
Monoamine-depleted D2−/− mice responded to a D1-like agonist
The ability of mice lacking D2 receptors to move could be mediated solely by the remaining D1 receptors. To address this question we attempted to reverse the monoamine depletion-induced akinesia with SKF38393, a D1-like selective receptor agonist (Setler et al., 1978). Mice were injected with either saline or 6 or 12 mg/kg SKF38393 and then placed immediately in the open-field apparatus for 1 hr. Analysis of the total horizontal distance traveled revealed that there was a significant genotype effect (F(4,170) = 10.23;p < 0.0001), with D2R−/− mice scoring higher than all other groups in response to SKF38393 and a treatment effect (F(2,170) = 4.30; p = 0.015) such that locomotor activity across genotypes was slightly stimulated by either dose of SKF38393 compared with saline administration (Fig.6). However, there was no significant genotype by drug interaction, perhaps because of the slightly higher baseline activity in the −/− mice after monoamine depletion. An analysis of covariance using the same data also failed to reveal a significantly greater response in the D2R−/− mice. These findings argue against D1 receptor supersensitivity as the major compensatory mechanism in the D2 receptor-deficient mice.
Monoamine-depleted D2−/− mice did not respond to a D2-like agonist
Because SKF38393 alone restored only a very small portion of the original activity levels, we attempted a more complete reversal of the akinesia with a combination of SKF38393 and quinpirole, a D2-like receptor agonist (Koller et al., 1987). A preliminary experiment had shown that 2 mg/kg quinpirole alone had no stimulatory effect on the locomotion of monoamine-depleted mice (data not shown). After the initial SKF38393 or saline injection trial, the same groups of mice were treated with their original SKF38393 dosage combined with 2 mg/kg quinpirole. Mice were then returned to the open-field apparatus for a second 1 hr period of monitoring. A comparison of the total horizontal distances scored after SKF38393 alone to the scores attained after SKF38393 and quinpirole, revealed that wild-type 129/SvEv, F2+/−, F2+/+, and wild-type C57Bl/6 mice all increased significantly (F(1,59) > 9.36;p < 0.003). In contrast, the F2−/− mice had no additional response to the combination of drugs. Unexpectedly, the F2+/− mice scored significantly higher (p < 0.0001 by Tukey post hoc tests) for total horizontal distance measured compared with all other groups at the lower (6 mg/kg) dose of SKF38393 plus quinpirole. A time course of the locomotor stimulatory action of the combined D1-like and D2-like agonists emphasizes the dramatic synergistic effect in the F2+/− mice compared with all other groups (Fig.7) and suggests that the differences in total horizontal distance shown in Figure 5 were underestimated by limiting the activity trial to 1 hr. A two-factor ANOVA comparison of saline and both doses of SKF38393 plus quinpirole revealed significant effects of genotype (F(4,170) = 19.8;p < 0.0001), treatment (F(2,170) = 53.11; p < 0.0001), and a gene × drug treatment interaction (F(8,170) = 5.97; p < 0.0001). The saline groups were lower than quinpirole combined with either dose of SKF38393 (p < 0.0001 by Tukey post hoc test). Only mice with two functional D2 receptor alleles showed a trend toward a greater response when given the combination of the higher dose SKF38393 and quinpirole, suggesting that the +/− mice had plateaued at their maximal possible response at the lower dose of SKF38393. The control groups of monoamine-depleted mice of all genotypes treated repeatedly with saline showed no spontaneous recovery, whatsoever, of locomotor activity in the course of the experiment.
DISCUSSION
D2 receptor-deficient mice are not a model of Parkinson’s disease
Our data in the present experiments and a previous report (Kelly et al., 1997) demonstrate that we have disrupted the D2 dopamine receptor gene in the mouse and confirm a role for the D2 receptor in specific central components of locomotion: initiation of movement, time spent in motion, and horizontal distance traveled. Rearing activity was also strongly influenced by the D2 receptor. The absence of D2 receptors did not affect the speed of horizontal movement or qualitatively alter the exploratory phase of locomotor activity in a novel environment. Additionally, we observed no postural abnormalities, catalepsy, tremor, or overt ataxia in the D2 receptor-deficient mice. Therefore, other than the decreased initiation of movement, in most respects the neurological syndrome of these mice differs considerably from Parkinson’s disease (Hornykiewicz, 1966); this finding is in disagreement with the interpretation of Baik et al. (1995), who analyzed an independently derived strain of F2 hybrid D2 receptor-deficient mice. It is not surprising that the presynaptic loss of dopamine found in Parkinson’s disease, or pharmacologically induced by monoamine depletion, is associated with more profound neurological deficits than the absence of a single subtype of dopamine receptor.
Acute antagonism of D2 receptors by haloperidol resulted in significant decreases in the horizontal distance traveled, initiation of movement, and time spent in motion for all genotypes except the D2 receptor-deficient −/− mice. Any possible antagonism of D3 (Freedman et al., 1994) and/or D4 (Asghari et al., 1995) receptors by haloperidol at the two doses used apparently had no net effect on locomotor activity in the D2R−/− mice. Rearing was also significantly decreased by haloperidol, except in the D2R−/− mice and wild-type 129/SvEv mice, which exhibited virtually no basal rearing activity.
Gene dosage effects in heterozygotes
It is noteworthy that drug-naive and haloperidol-treated mice heterozygous for the targeted D2 allele consistently demonstrated phenotypic measures that were intermediate to those of the +/+ and −/− mice. Scatchard analysis of [3H]nemonapride binding indicated previously that the affinity of the D2 receptor for this ligand was unchanged in +/− mice, but the total number of binding sites was reduced by ∼50% (Kelly et al., 1997). Therefore, it does not appear that the loss of one allele and half of the receptor binding sites caused a compensatory upregulation of the remaining normal allele. If postsynaptic D2 receptors were present in excess on the cell membrane it might be predicted that +/− mice with half the receptor number would have no phenotype, but this was not the case. Therefore, the decreased locomotor function displayed by +/− mice in this study is consistent with a model for the nigrostriatal dopamine system that includes no spare postsynaptic D2 receptors, similar to the conclusions drawn for the abundance of D2 receptors on pituitary lactotrophs (Meller et al., 1991). The D2 receptor +/− phenotype is also in agreement with antisense knock-down experiments in which moderate reductions in D2 receptors, as assessed by ligand-binding studies, led to significant phenotypic effects (Zhang and Creese 1993; Zhou et al., 1994; Silvia et al., 1994).
The decreased Bmax in the +/− mice may be particularly relevant when considering dopamine transporter regulation, downstream second messenger effectors, and regulation of dopamine biosynthesis through dopamine D2R-like autoreceptors. We found no evidence for altered dopamine biosynthesis in either the D2+/− or −/− mice based on the tissue content of dopamine and its major metabolites. However, further studies of dopamine turnover and release are warranted to more fully examine the issue of dopamine biosynthesis, particularly because recent studies provide little support for the concept of compensatory D3 autoreceptors on SN dopamine neurons (Xu et al., 1997). The unexpectedly high locomotor response of monoamine-depleted D2R+/− mice to the combined treatment by D1- and D2-like agonists further emphasizes the importance of D2 receptor gene dosage to motor function. The mechanism underlying this functional supersensitivity is unknown but probably occurs at a postreceptor level and develops in response to continued dopamine signaling through a chronically reduced number of D2 receptors. Further analyses of the heterozygous mice may prove valuable in understanding the role of D2 receptor signaling in normal brain function and plasticity at dopaminergic synapses.
Importance of genetic background in gene deletion experiments
Our study is a specific example of the importance of genetic background in the evaluation of complex behavioral traits in mice generated by targeted mutagenesis (Gerlai, 1996; Lathe, 1996; Banbury Conference on Genetic Background in Mice, 1997). The vast majority of gene knock-out experiments have used a diverse collection of ES cells derived from substrain 129 mice (Simpson et al., 1997; Threadgill et al., 1997), and the phenotypic analysis is often confined to F2 hybrid mice derived by crossing the 129 ES cell-derived chimeras with another inbred strain, typically C57BL/6. This issue was particularly germane in the present situation, because wild-type 129/SvEv mice with functional D2 receptors were a virtual phenocopy of the predicted locomotor deficits caused by the loss of D2 receptors. In particular, the 129/SvEv mice had markedly reduced scores for initiation of spontaneous movement, rearing, and rotarod performance compared with C57BL/6 mice. The low scores of the 129 parental strain produced a floor effect confounding an interpretation of the independent contribution of D2 receptors to the same measures. Therefore, a combined analysis of the parental strains and congenic strains carrying the mutated D2 receptor allele was essential to discriminate between the phenotypes resulting from either the intentional mutation in the D2 receptor gene or other polygenic loci contributed by the parental inbred strains (Flint et al., 1995).
This combined analysis revealed some surprising findings relevant to motor coordination on a rotarod. Most wild-type 129/SvEv and F2−/− mice were unable to perform the rotarod test after many trials, whereas wild-type C57BL/6 and congenic B6 D2R−/− mice successfully learned to perform this task. Because the F2−/− mice have undergone only a small number of meiotic crossover events on chromosome 9 (the location of the D2 receptor gene Drd2 in mice), it is probable that many, but not all, genes from the 129 parental chromosome 9 are still linked to the targeted D2 receptor alleles, particularly in the differential segment (Banbury Conference on Genetic Background in Mice, 1997). Consequently, it is also probable that substrain 129 gene alleles other thanDrd2 are largely responsible for the poor rotarod performance of F2−/− mice and the bimodal distribution of F2+/− mice scores. The fact that the congenic B6 D2R−/− mice were able to learn the rotarod task, albeit with a lower aptitude and a shallower learning curve than their siblings with intact D2 receptors, proves that the D2 receptor deficiency per se was not responsible for the failure of the F2−/− mice on the rotarod, as concluded in a previous report (Baik et al., 1995). We believe that a differential contribution of substrain 129 gene alleles is the major reason for the apparent discrepancies in phenotypes between that report and our own. By extension of these examples, it is possible that background strain effects have influenced the interpretation of other locomotor behavioral phenotypes described for strains of mice with dopamine D1 and D3 receptor genes that have been mutated by similar techniques (Xu et al., 1994; Drago et al., 1994;Accili et al., 1996; Xu et al., 1997).
Developmental adaptations in D2 receptor-deficient mice
The net reduction in locomotor activity resulting from the chronic absence of D2 dopamine receptors in the mutant mice was significantly less than that induced by the acute pharmacological blockade of D2-like receptors by haloperidol in wild-type mice, regardless of genetic background. Therefore, we hypothesize that one or more adaptations have occurred in the CNS of the mutant mice to account for their relatively good locomotor function. D2−/− mice were still dependent on the function of monoaminergic neural circuits, because the combined blockade of dopaminergic, serotoninergic, and catecholaminergic synaptic activity with reserpine and AMPT produced akinesia. Significant reversal of akinesia was readily accomplished in both wild-type 129/SvEv and C57BL/6 mice by the simultaneous administration of two subtype-selective, direct dopamine agonists, SKF38393 (D1 and D1B) and quinpirole (D2, D3, and D4). Based on these observations, we predicted that dopaminergic activation of the remaining D1, D1B, D3, and D4 receptors in D2R−/− mice would be sufficient to account for their basal locomotor activity. If this prediction were true, the combination of SKF38393 and quinpirole would have reversed the reserpine-induced akinesia in D2−/− mice. In fact, the D2−/− mice demonstrated only a small response to the D1-like agonist with no additive or synergistic effect by the D2-like agonist, indicating that the primary or sole adaptation in the D2−/− mice is not the upregulation of other components of central dopaminergic circuits. There was actually a decrease in the number of D1 binding sites assessed with a radiolabeled antagonist, similar to the downregulation of D1 receptors in primate cortex, but not neostriatum, after chronic treatment with D2-like receptor antagonists (Lidow et al., 1997). An additional experiment with drug-naive mice administered 10 mg/kg of the full D1R agonist SKF81297 also failed to demonstrate D1R-supersensitivity, although the D2R−/− mice responded to the treatment with increased activity [60 min total horizontal distance scores (mean ± SEM): saline-treated D2R−/−, 5824 ± 1278 cm; SKF-treated D2R−/−, 13,950 ± 1791 cm; saline-treated D2R+/+, 9940 ± 682 cm; and SKF-treated D2R+/+, 20,730 ± 1329 cm].
Further studies are necessary to fully analyze the possible contribution of nonD2 receptors and to determine the importance of serotonin, catecholamine, or other nonmonoamine neurotransmitter systems in the compensatory mechanisms. One attractive hypothesis would be a downregulation of adenosine A2A receptors that normally oppose the function of dopamine D2 receptors at common postsynaptic sites (Ferré et al., 1991; Svenningsson et al., 1997). In the future, a conditional gene-targeting system for the D2 receptor will also clarify which adaptations to the absence of D2 receptors are determined by irreversible organizational plasticity in the developing brain and which occur activationally in the mature brain.
Footnotes
This work was supported by Public Health Service Grants DA07262 (M.A.K.), NS09199 (G.A.G.), AG06434 (G.A.G.), and DA09620 (D.K.G.), the Department of Veterans Affairs (T.J.P.), Parke Davis Pharmaceuticals (M.J.L.), and the Lucille P. Markey Charitable Trust (M.J.L. and D.K.G.). We thank O. Rønnekleiv for assistance with D1 ligand autoradiography, E. Johnston for help with behavioral studies, S. D. Dickinson for statistical analyses, and M. Geyer and N. Zahniser for critical reading of this manuscript.
Correspondence should be addressed to Dr. Malcolm J. Low, Vollum Institute, L474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97201.
Dr. Rubinstein’s present address: Instituto de Investigaciones en Ingeneria Genetica y Biologia Molecular, Consejo Nacional de Investigaciones Cientı́ficas y Técnicas, University of Buenos Aires, Argentina.