Article Figures & Data
Figures
- Fig. 1.
Representative autoradiogram of mRNA differential display with an anchor primer, T12MC, and an arbitrary primer, 5′-TGTACGAAAT-3′. Arrow shows cDNA band ofLot1. Samples were collected from SCN at P2, P10, P20, and P50 and from the cerebral cortex at P50. SCN, Suprachiasmatic nucleus; CCx, cerebral cortex.
- Fig. 2.
A, Amino acid sequence of LOT1. Zinc-finger motifs are highlighted by bold underlining.Boxes indicate the Cys and His of zinc finger.B, Northern blot analysis of Lot1 in rat tissue RNA. Two micrograms of mRNA in P50 rat tissues were loaded on each lane. β-actin served as a loading control.C, In situ hybridization using antisense probe and sense probe in P50 rat brain. Photomicrographs were taken using Bio-max film (Kodak). Arc, Arcuate hypothalamic nucleus; LS, lateral septal nucleus; Me, medial amygdaloid nucleus; OVLT, organum vasculosum lamina terminalis; PirCx, piriform cortex;Pur, Purkinje cell layer. Scale bars, 2 mm.
- Fig. 3.
Expression of Lot1 mRNA in fetal rat (E20) detected by in situ hybridization using antisense probe. Sense controls performed in the adjacent sections showed no signals. Scale bar, 2 mm.
- Fig. 4.
Expression of Lot1 mRNA in the developing SCN. A, Film autoradiographic image ofin situ hybridization of Lot1 mRNA in the SCN of E20, P1, P3, P5, P7, P10, P20, and P50 rats. Scale bars, 2 mm.B, Developmental change of the relative amount ofLot1 mRNA in the SCN. The value at P3 is adjusted to 100.
- Fig. 5.
Dark-field emulsion image of Lot1mRNA in P5 (A) and P10 (B,C) rat SCN. SCN is outlined bybroken lines in A and B.C, Bright-field high-power photomicrograph of the dorsomedial SCN in B. oc, Optic chiasma;v, third ventricle. Scale bars: A,B, 100 μm; C, 10 μm.
- Fig. 6.
Diurnal and circadian profiles ofLot1 mRNA levels in the SCN of P10 rats. Quantitativein situ hybridization analysis of Lot1mRNA in the P10 rat brain under 12 hr LD and total darkness (DD) is shown. Values are means ± SEM (n = 7). *p < 0.05, compared with the value at ZT4 or CT4 (ANOVA). Representative sections are shown in top panels; SCN is indicated by arrows. Scale bars, 2 mm.
