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ARTICLE

GDNF Protection against 6-OHDA–Induced Reductions in Potassium-Evoked Overflow of Striatal Dopamine

Wayne A. Cass and Michael W. Manning
Journal of Neuroscience 15 February 1999, 19 (4) 1416-1423; DOI: https://doi.org/10.1523/JNEUROSCI.19-04-01416.1999
Wayne A. Cass
1Department of Anatomy and Neurobiology, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298
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Michael W. Manning
1Department of Anatomy and Neurobiology, University of Kentucky College of Medicine, Lexington, Kentucky 40536-0298
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Abstract

Glial cell line–derived neurotrophic factor (GDNF), when administered before 6-hydroxydopamine (6-OHDA), has been shown to prevent the reduction in nigral dopamine (DA) levels and tyrosine hydroxylase–positive neurons normally observed after 6-OHDA lesions. The present study examined the ability of GDNF to prevent 6-OHDA–induced reductions in striatal DA release and reductions in striatal and nigral DA levels. GDNF (10 μg), or vehicle, was injected into the right nigra of anesthetized male Fischer-344 rats and was followed 6 hr later by intranigral 6-OHDA or saline. Three to four weeks later the animals were anesthetized with urethane and prepared for in vivo electrochemistry. Potassium-evoked overflow of DA was dramatically decreased in the right striatum of the vehicle + 6-OHDA–treated animals. GDNF appeared to prevent the reduction in evoked overflow of DA in the right striatum of the 6-OHDA–treated animals. However, in comparison with that in animals that received GDNF + saline, the overflow of DA was significantly reduced in the GDNF + 6-OHDA animals. Similarly, although nigral levels of DA were above normal in the GDNF + 6-OHDA–treated animals, they were below DA levels found in GDNF + saline–treated rats. Striatal DA levels were partially protected by GDNF. In animals examined 10–12 weeks after the GDNF and 6-OHDA treatments, the apparent protective ability of GDNF on the evoked overflow of DA in the striatum was diminished. Thus, although intranigral GDNF can prevent 6-OHDA–induced reductions in nigral DA levels, long-term protection of the evoked overflow of DA in the striatum is minimal.

  • GDNF
  • 6-hydroxydopamine
  • striatum
  • dopamine
  • neurotrophic factor
  • in vivo electrochemistry
  • neurotoxicity

Parkinson’s disease is characterized by the progressive degeneration of nigrostriatal dopamine (DA) neurons. Drugs or other compounds that can slow down this degeneration or partially restore dopaminergic functioning would therefore be of potential therapeutic benefit. One such possible compound is glial cell line–derived neurotrophic factor (GDNF).In vitro, GDNF promotes the survival and differentiation of embryonic, mesencephalic DA neurons (Lin et al., 1993; Hou et al., 1996). In normal young and aged animals, in vivo GDNF can augment nigral DA levels and tyrosine hydroxylase (TH)-positive fiber density (Gash et al., 1995; Hudson et al., 1995; Martin et al., 1996) and can increase evoked overflow of DA in the striatum (Gash et al., 1995; Hebert et al., 1996; Hebert and Gerhardt, 1997). When given after administration of the neurotoxins 6-hydroxydopamine (6-OHDA) (Hoffer et al., 1994; Bowenkamp et al., 1995; Lapchak et al., 1997; Rosenblad et al., 1998) or 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (Tomac et al., 1995; Gash et al., 1996; Miyoshi et al., 1997; Zhang et al., 1997), GDNF can partially ameliorate the behavioral and neurochemical deficits normally observed in these animals. All of these studies emphasize the potential therapeutic application of GDNF for treating Parkinson’s disease.

Several studies have examined the neuroprotective potential of GDNF against lesions of the nigrostriatal DA system. For instance, GDNF has been shown to provide varying degrees of protection to DA neurons against MPTP toxicity (Tomac et al., 1995; Kojima et al., 1997), 6-OHDA toxicity (Kearns and Gash, 1995; Sauer et al., 1995; Winkler et al., 1996; Choi-Lundberg et al., 1997; Sullivan et al., 1998), and methamphetamine toxicity (Cass, 1996). In addition, GDNF can also reduce degeneration of mesencephalic DA neurons after transection of the medial forebrain bundle (Beck et al., 1995). However, the extent of protection by GDNF varies markedly in different studies, pointing out that several factors are important for determining the success of GDNF in protecting DA neurons. These factors include type and extent of the lesion as well as dose, site of injection, and the time course for delivery of GDNF.

One recent study has shown that a single intranigral injection of GDNF, given 6 hr before intranigral 6-OHDA, can prevent completely the 6-OHDA–induced loss of DA neurons and DA content in the substantia nigra (Kearns et al., 1997). However, whether or not the functional dynamics of DA release and uptake in the striatum of these animals is also preserved is unclear. The purpose of the present study was to evaluate further the protective effects of GDNF, given 6 hr before a 6-OHDA lesion, on the nigrostriatal DA system. In vivoelectrochemistry was used to measure potassium-evoked overflow of DA in the striatum at both 3–4 and 10–12 weeks after unilateral GDNF and 6-OHDA administration into the nigra. In addition, tissue levels of DA in the striatum and substantia nigra were determined at the conclusion of each experiment to evaluate the extent of the lesion produced by 6-OHDA and the ability of GDNF to preserve DA levels.

MATERIALS AND METHODS

Animals. Forty-eight male Fischer-344 rats (Harlan Sprague Dawley, Indianapolis, IN) weighing 230–330 gm were used for these experiments. They were housed in groups of two under a 12 hr light/dark cycle with food and water available ad libitum. All animal-use procedures were in strict accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care and Use Committee at the University of Kentucky.

GDNF and 6-OHDA administration. Rats were anesthetized with sodium pentobarbital (50 mg/kg, i.p.) and placed into a stereotaxic frame. All surgery was performed using aseptic conditions. The skull was exposed, and a small hole was drilled in the skull over the right substantia nigra. Human recombinant GDNF (10 μg in 2 μl of vehicle solution; Synergen, Boulder, CO, and Amgen, Thousand Oaks, CA) or 2 μl of vehicle (10 mm citrate buffer with 150 mm NaCl, pH 5) was injected into the dorsal region of the right substantia nigra (5.4 mm posterior to bregma, 2.2 mm lateral from the midline, and 7.3 mm below the surface of the cortex) using a Hamilton or an SGE syringe with a 26 gauge needle. The rate of injection was 0.2 μl/min. The needle was left in place for an additional 5 min after the injection and then slowly withdrawn. The scalp was closed with wound clips. Six hours later the animal was readministered sodium pentobarbital (25 mg/kg, i.p., or more if needed) and placed back into the stereotaxic frame. 6-OHDA hydrobromide (8 μg in 2 μl of vehicle; Sigma, St Louis, MO) or 2 μl of saline vehicle (0.02% ascorbic acid in 0.9% saline) was injected using the same coordinates and protocol described above. After the injection, gelfoam was placed in the burr hole, the incision was sutured closed, and the animals were returned to their home cages for recovery.

In vivo electrochemistry. Three to four or 10–12 weeks after the intranigral injections, the animals were anesthetized with urethane (1.25–1.5 gm/kg, i.p.), placed into a stereotaxic frame, and prepared for in vivo electrochemical recordings as described previously (Cass, 1996, 1997).

Nafion-coated single-carbon fiber electrodes were constructed and calibrated in vitro (Cass, 1996, 1997). The electrodes were attached to single-barrel micropipettes (tip diameter, 10–20 μm) with dental wax so that the tips of the pipettes and the electrodes were separated by 260–300 μm. The micropipettes were filled with a solution containing 70 mm KCl, 79 mm NaCl, and 2.5 mm CaCl2, pH 7.4.

High-speed chronocoulometric electrochemical measurements were made continuously at 5 Hz and averaged to 1 Hz using an IVEC-10 system (Medical Systems Corporation, Greenvale, NY). The applied oxidation potential was +0.55 V for 100 msec (vs Ag/AgCl reference electrodes), and the resting potential was 0.0 V for 100 msec. The oxidation and reduction currents were digitally integrated during the last 80 msec of each 100 msec pulse. Electrode assemblies were initially positioned in the dorsal striatum (1.2–1.6 mm anterior to bregma, 2.2–2.3 mm lateral from the midline, and 3.5 mm below the surface of the brain). The baseline electrochemical signal was allowed to stabilize (5–10 min), and then 125–150 nl of potassium solution (70 mm K+) was applied by pressure ejection (Picospritzer II; General Valve, Fairfield, NJ) during the recordings to evoke the release of DA (see Fig.1B). The volume of fluid injected was monitored by determining the amount of fluid displaced from the micropipette using a dissection microscope fitted with a reticule eyepiece and was based on previous calculations that there are ∼250 nl of solution in a 1 mm segment of the micropipette (Friedemann and Gerhardt, 1992). After the signal had returned to baseline, the electrode and micropipette assembly was lowered by 0.5 mm. The new baseline was allowed to stabilize, and then 125–150 nl of potassium solution was applied again. The potassium applications were repeated at 0.5 mm steps to map the striatum and nucleus accumbens (see Fig. 1A). A single recording pass was made in each hemisphere of each animal. The order of the passes was alternated between animals in each group. There was no difference in the amount of potassium solution applied between the right and left sides of the brain or between the different treatment groups.

Tissue collection and HPLC analysis. At the end of the experiments, the animals were killed by decapitation while still anesthetized with urethane. The brains were rapidly removed and chilled in ice-cold saline. A coronal slice of brain ∼2 mm thick at the level of the recording tracts was made with the aid of a chilled brain mold (Rodent Brain Matrix; ASI Instruments, Warren, MI). The location of each recording electrode was confirmed by noting blood left in the recording track. The striatum was dissected from each half of the slice as a single piece. The substantia nigra was similarly dissected from both sides of a 2-mm-thick coronal slice through the midbrain. The tissue pieces were placed in preweighed vials, weighed, and frozen on dry ice. Samples were stored at −80°C until assayed by HPLC. Tissue levels of DA were determined using HPLC with electrochemical detection as described previously (Cass, 1996, 1997). Retention times of standards were used to identify peaks, and peak heights were used to calculate the recovery of internal standard (dihydroxybenzylamine) and the amount of DA. Results were expressed as micrograms per gram wet weight of tissue.

Data analysis. The electrodes used in this study, although relatively insensitive to ascorbic acid because of the Nafion coating (Gerhardt et al., 1984), can still detect serotonin (5-HT) if the levels are high enough. To confirm that the responses detected were attributable primarily to DA, we recorded both the reduction and oxidation currents and calculated the ratio of the reduction current to the oxidation current for each K+-induced response. The electrodes used in this study exhibit reduction/oxidation current ratios of 0.4 or greater for DA and ratios of 0.1–0.2 for 5-HT (Luthman et al., 1993; Cass, 1997). Ascorbic acid, if detected, is not reduced at the potentials used and therefore gives a reduction/oxidation current ratio of 0.0. All of the responses included in the Results had reduction/oxidation ratios of at least 0.4, indicating that DA was the predominant compound detected by the electrodes after potassium application.

For statistical analysis of the electrochemistry data two parameters were analyzed, (1) the maximum amplitude of the signals resulting from the application of potassium and (2) the clearance rate of the signals [the slope of the linear, declining portion of the signal between theT20 and T60 time points (see Fig. 1B), with the T20and T60 points representing the time points at which the signal has declined by 20 and 60% of the maximum amplitude]. The clearance rate is an estimate of the maximal slope of the declining phase of the signal and is related to those processes responsible for removing DA from the extracellular space. This includes predominantly DA reuptake, but diffusion (and possibly metabolism) will play a greater role when there are fewer functional DA transporters in the region of the recording electrode (Wightman and Zimmerman, 1990;Cass et al., 1993; Suaud-Chagny et al., 1995; Garris et al., 1997). Signal amplitude, clearance rate, and tissue monoamine concentration were analyzed using t tests or ANOVAs, followed by Newman–Keuls post hoc comparisons as indicated in the Results.

RESULTS

Three to four weeks after the lesion

Locally applied potassium produced signals with reduction/oxidation current ratios characteristic for DA at all recording depths in the striatum and nucleus accumbens of all animals. Representative responses from the right striatum of GDNF + 6-OHDA– and of vehicle + 6-OHDA–treated rats are shown in Figure1B. On the basis of observations of the placement of the electrode tracts in the present study and previous experience with recording from the striatum and nucleus accumbens (Cass et al., 1992, 1993; Cass and Gerhardt 1994,1995), recordings at a depth of 3.5–6.0 mm below the surface of the cortex are contained within the striatum (Fig. 1A). Recordings at a depth of 6.5 mm are approximately at the junction of the striatum and the nucleus accumbens, whereas recordings at depths of 7.0 and 7.5 mm are within the nucleus accumbens.

Fig. 1.
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Fig. 1.

A, Illustration of the locations of the recording sites for the in vivo electrochemical recordings. Data were collected at 0.5 mm steps throughout the dorsoventral extent of the striatum and nucleus accumbens (NAc) (3.5–7.5 mm below the surface of the brain) on both sides of the brain. This diagram represents a coronal section of the right hemisphere of the forebrain ∼1.2 mm anterior to bregma.B, Representative signals from the dorsal striatum of a GDNF + 6-OHDA–treated rat (GDNF) and a vehicle + 6-OHDA–treated rat (Vehicle) showing the potassium-evoked overflow of DA. Potassium (150 nl; 70 mmK+) was applied at the arrowhead at time 0 in both cases. The solid lines are the oxidation current responses, and the dashed lines below eachsolid line are the corresponding reduction current responses. The reduction/oxidation current ratios (0.83 and 0.70 for the GDNF and Vehicle responses, respectively) indicate that the predominate electroactive species detected was DA. The T20 andT60 time points used for calculating clearance rate are indicated on the oxidation curve of both recordings and are labeled on the curve from the GDNF + 6-OHDA–treated rat.

Signal amplitudes from animals treated with vehicle or GDNF followed 6 hr later by saline injections are shown in Figure2. In the vehicle + saline–treated animals, there was no difference at any individual recording depth between the signals recorded from the treated side of the brain and the contralateral side. However, within each side, signal amplitude did drop off at the deeper recording sites, particularly in the region of the nucleus accumbens (p < 0.05, one-way repeated measures ANOVA). This is similar to what has been reported previously (Cass, 1996, 1997; Hebert et al., 1996) and is likely caused by the decreased density of dopaminergic innervation to the nucleus accumbens and adjacent striatum. In the GDNF + saline–treated animals, there was a tendency for signal amplitude to be augmented on the GDNF-treated side of the brain compared with the contralateral side (Fig. 2). However, this increase did not reach statistical significance. As with the vehicle + saline–treated animals, there was a significant difference in signal amplitude over depth on both the ipsilateral and contralateral sides of the GDNF + saline–treated rats (one-way repeated measures ANOVA).

Fig. 2.
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Fig. 2.

Summary of potassium-evoked DA signal amplitude throughout the striatum and nucleus accumbens of animals treated with vehicle (top) or GDNF(bottom) followed 6 hr later by saline. All injections were made into the right substantia nigra. In vivoelectrochemical recordings were made 3–4 weeks after the intranigral injections. The data shown are mean (± SEM) values for eight animals per group. The data were analyzed using two-factor ANOVA with side of the brain and depth of the recording as within factors.F scores for the vehicle + saline group are as follows: side F = 0.13, p > 0.05; depthF = 13.31, p < 0.001; and interaction F = 0.36, p > 0.05. GDNF + saline group F scores are as follows: side F = 2.16, p > 0.05; depth F = 12.02, p < 0.001; and interaction F = 0.63,p > 0.05.

In the animals treated with vehicle followed by 6-OHDA, there was a dramatic decrease in the amplitude of the signals on the lesioned side (Fig. 3). These reductions were significant in the dorsal striatum but not in the nucleus accumbens. In the GDNF + 6-OHDA–treated animals, the amplitude of the potassium-evoked signals tended to be decreased in the dorsal striatum on the treated side of the brain, but there was no main effect of treatment in the overall ANOVA (Fig. 3). However, if the signal amplitudes from the treated side of the brain of the GDNF + 6-OHDA group are compared with that from the treated side of the GDNF + Saline group (Fig. 2), there is a significant effect of treatment (two-way ANOVA with depth below the brain surface as a within factor; treatmentF = 6.45, p = 0.024; depthF = 8.78, p < 0.001; and interactionF = 2.35, p = 0.023). Similarly, there is also a significant effect of treatment between the treated sides of the GDNF + 6-OHDA group and the vehicle + 6-OHDA group (treatmentF = 11.17, p = 0.005; depthF = 1.51, p = 0.16; and interactionF = 2.01, p = 0.051).

Fig. 3.
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Fig. 3.

Summary of potassium-evoked DA signal amplitude throughout the striatum and nucleus accumbens of animals treated with vehicle (top) or GDNF(bottom) followed 6 hr later by 6-OHDA. All injections were made into the right substantia nigra. In vivo electrochemical recordings were made 3–4 weeks after the intranigral injections. The data shown are mean (± SEM) values for eight animals per group. The data were analyzed using two-factor ANOVA with side of the brain and depth of the recording as within factors.F scores for the vehicle + 6-OHDA group are as follows: side F = 56.65,p < 0.001; depth F = 7.07,p < 0.001; and interaction F = 7.83, p < 0.001. GDNF +6-OHDA group F scores are as follows: side F = 2.94, p > 0.05; depthF = 12.03, p < 0.001; and interaction F = 3.13, p < 0.01. *p < 0.05 versus the left side at the same depth (Newman–Keuls post hoc comparisons).

The ANOVA results indicated that the 6-OHDA treatment in the vehicle + 6-OHDA animals decreased DA release in the dorsal striatum but not in the nucleus accumbens. Because only the striatum was effected, data from recordings at depths from 3.5 to 6.0 mm below the surface of the brain were averaged together for each animal to obtain an overall value for the striatum. When this was done, both signal amplitude and clearance rate were decreased on the treated side of the vehicle + 6-OHDA group by 83% (Fig. 4). On the right side of the GDNF + 6-OHDA–treated animals, signal amplitude was decreased by 41%, and clearance rate was decreased by 51% (Fig. 4). Both signal amplitude and clearance rate were significantly greater on the right side of the GDNF + 6-OHDA–treated animals than on the right side of the vehicle + 6-OHDA–treated animals.

Fig. 4.
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Fig. 4.

Signal amplitudes (top) and clearance rates (bottom) from the striatum of rats treated with GDNF or vehicle (Veh) followed 6 hr later by 6-OHDA or saline. All injections were made into the right substantia nigra. In vivoelectrochemical recordings were made 3–4 weeks after the injections. The data from each recording depth in the striatum (3.5–6.0 mm below the surface of the cortex) of each animal were averaged together. The values shown are mean (± SEM) for eight animals per group. *p < 0.05 versus the left side of the same group; +p < 0.05 versus the right side of the vehicle +6-OHDA group (two-factor ANOVA with side of the brain as a within factor; Newman–Keuls post hoccomparisons).

DA levels in the striatum and substantia nigra are shown in Figure5. In the striatum there was a 19% decrease in DA levels on the treated side of the GDNF + saline animals. In the vehicle + 6-OHDA animals, there was an 81% decrease in DA levels on the treated side, and in the GDNF + 6-OHDA animals, there was a 48% decrease on the treated side. In the substantia nigra of the GDNF + saline–treated animals, there was a 92% increase in DA content on the right side. In the vehicle + 6-OHDA group, nigral DA levels were reduced by 76% on the lesioned side, whereas on the treated side of the GDNF + 6-OHDA–treated animals, there was a trend for an increase in nigral DA levels. In both the striatum and the nigra, DA levels on the right side of the GDNF + 6-OHDA–treated animals were significantly greater than levels on the right side of the vehicle + 6-OHDA–treated animals.

Fig. 5.
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Fig. 5.

DA levels in the striatum (top) and substantia nigra (bottom) from rats treated 3–4 weeks earlier with intranigral GDNF or vehicle (Veh) followed 6 hr later by intranigral6-OHDA or saline. All injections were made into the right side of the brain. The data shown are mean (± SEM) values from eight animals per group. *p < 0.05 versus the left side of the same group; +p < 0.05 versus the right side of the vehicle + 6-OHDA group (two-factor ANOVA with side of the brain as a within factor; Newman–Keuls post hoc comparisons).

Ten to twelve weeks after the lesion

The results at 3–4 weeks after treatment suggest that the GDNF is partially protecting striatal DA terminals against the effects of 6-OHDA and possibly providing complete protection to DA cell bodies in the substantia nigra. However, another possibility is that the GDNF is just upregulating the remaining nigrostriatal neurons rather than protecting against the effects of 6-OHDA. To examine this possibility, a second set of experiments was performed in which rats were treated with GDNF + saline or with GDNF + 6-OHDA and 10–12 weeks were allowed to elapse before the in vivo electrochemistry experiments and the determination of striatal and nigral DA content.

In animals treated with GDNF + saline 10–12 weeks earlier, there was no difference in the potassium-evoked signal amplitude between the two sides of the brain at any recording depth (Fig.6). In contrast, in animals treated with GDNF + 6-OHDA 10–12 weeks earlier, there was a significant decrease in signal amplitude in the treated dorsal striatum compared with the contralateral side (Fig. 6). Overall signal amplitudes and clearance rates from the striatum of the rats treated 10–12 weeks earlier are shown in Figure 7. There was no difference in average amplitude or clearance rate between the right and left striata in the GDNF + saline–treated animals. However, in the GDNF + 6-OHDA–treated animals, there was a 70% decrease in signal amplitude and a 69% decrease in clearance rate on the treated side of the brain compared with the contralateral side. Signal amplitudes and clearance rates from the treated side of these animals were significantly lower than those from the treated side of the GDNF + 6-OHDA group of animals that were examined at 3–4 weeks after treatment (Fig. 4; p < 0.05, unpaired ttests).

Fig. 6.
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Fig. 6.

Summary of potassium-evoked DA signal amplitude throughout the striatum and nucleus accumbens of animals treated withGDNF followed 6 hr later by saline (top) or 6-OHDA (bottom). All injections were made into the right substantia nigra. In vivoelectrochemical recordings were made 10–12 weeks after the injections. The data shown are mean (± SEM) values for eight animals per group. The data were analyzed using two-factor ANOVA with side of the brain and depth of the recording as within factors. F scores for the GDNF + saline group are as follows: sideF = 0.29, p > 0.05; depthF = 7.01, p < 0.001; and interaction F = 0.60, p > 0.05. GDNF + 6-OHDA groupF scores are as follows: side F = 16.51, p < 0.01; depth F = 5.59, p < 0.001; and interactionF = 6.24, p < 0.001. *p < 0.05 versus the left side at the same depth (Newman–Keuls post hoc comparisons).

Fig. 7.
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Fig. 7.

Signal amplitudes (top) and clearance rates (bottom) from the striatum of rats treated with GDNF followed 6 hr later by6-OHDA or saline. All injections were made into the right substantia nigra. In vivo electrochemical recordings were made 10–12 weeks after the injections. The data from each recording depth in the striatum (3.5–6.0 mm below the surface of the cortex) of each animal were averaged together. The values shown are mean (± SEM) for eight animals per group. *p < 0.05 versus the left side of the same group (two-factor ANOVA with side of the brain as a within factor; Newman–Keuls post hoccomparisons).

On the right side of the animals treated 10–12 weeks earlier with GDNF + saline, DA levels were decreased by 43% in the striatum and increased by 101% in the substantia nigra (Fig.8). On the right side of the GDNF + 6-OHDA group, DA levels were decreased by 75% in the striatum and increased by 72% in the nigra compared with the contralateral side.

Fig. 8.
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Fig. 8.

DA levels in the striatum (top) and substantia nigra (bottom) from rats treated 10–12 weeks earlier with intranigral GDNF followed 6 hr later by intranigral 6-OHDA or saline. All injections were made into the right side of the brain. The data shown are mean (± SEM) values from eight animals per group. *p < 0.05 versus the left side of the same group (two-factor ANOVA with side of the brain as a within factor; Newman–Keuls post hoccomparisons).

DISCUSSION

GDNF is a potent neurotrophic factor that has significant effects on dopaminergic, as well as nondopaminergic, neurons in the mammalian CNS (Cass et al., 1998). The positive effects of GDNF on dopaminergic systems have led to the suggestion that GDNF may prove useful for the treatment of Parkinson’s disease. It may be able to halt or slow down progression of the disease or even partially restore lost function. In the present study the protective effects of GDNF on the functioning of striatal DA terminals were evaluated in a rodent model of Parkinson’s disease. Within the constraints of the paradigm used in the present study, our results indicate that although a single intranigral injection of GDNF may protect DA neuron cell bodies in the substantia nigra, the release of DA in the striatum is only minimally protected.

The time course for injection of GDNF, 6 hr before 6-OHDA, was chosen on the basis of previous work that demonstrated that this time point preserved TH-positive cell bodies in the nigra to a greater extent than did 0, 1, 12, or 24 hr time points (Kearns et al., 1997). In the present study, GDNF injected into the nigra 6 hr before intranigral 6-OHDA prevented 6-OHDA–induced reductions in nigral DA levels at both 3–4 and 10–12 weeks after treatment. These results, along with the previous results indicating that this GDNF treatment completely prevents 6-OHDA–induced loss of DA cell number in the nigra (Kearns et al., 1997), suggest that a single injection of GDNF can provide complete protection to DA neuron cell bodies in the substantia nigra.

Other investigators have also shown that GDNF can provide near complete to complete protection of DA cell bodies in the nigra. Supranigral administration of GDNF every other day for 4 weeks, starting on the day of an intrastriatal 6-OHDA lesion, completely prevented loss of cells in the substantia nigra, whereas a single injection 7 d after the lesion partially prevented cell loss (Sauer et al., 1995). When GDNF was injected into the striatum both before and after the intrastriatal administration of 6-OHDA, the number of TH-positive neurons in the substantia nigra was almost completely preserved (Shults et al., 1996). Multiple supranigral injections of GDNF for 37 d after intrastriatal 6-OHDA led to a 78% sparing of nigral TH-positive neurons at 5 months after the lesion (Winkler et al., 1996). However, in these same animals there was no protective effect of GDNF on striatal DA terminals (Winkler et al., 1996). Ipsilateral administration of GDNF supranigrally and intraventricularly immediately before injection of 6-OHDA into the medial forebrain bundle led to a 77–80% survival of TH-positive neurons in the midbrain and a 77% sparing of DA levels in the striatum 13 weeks after the lesion (Sullivan et al., 1998). These studies, like the present one, all support that GDNF can substantially protect nigral DA neurons from the neurotoxic effects of 6-OHDA.

Although the present GDNF treatment protected DA cell bodies, the results of the in vivo electrochemical studies suggest that presynaptic dopaminergic function is only partially protected. In the GDNF + 6-OHDA–treated rats, potassium-evoked overflow of DA and clearance of DA in the striatum decreased from 3–4 to 10–12 weeks after treatment. By 10–12 weeks after treatment, these indices of DA terminal function were not significantly different from those in the vehicle + 6-OHDA–treated animals at 3–4 weeks after the lesion (unpaired t tests). Striatal DA levels declined in a similar manner over time. The decrease in dopaminergic functioning in the striatum over time suggests that some of the protective effects of GDNF in the striatum may actually represent an upregulation in the functioning of remaining DA terminals that declines over time. It has been shown recently that GDNF can increase the quantal size for release of DA from cultured midbrain neurons (Pothos et al., 1998). If this is a transient phenomenon that lasts only a few weeks, then this could explain the present results. Another possibility is that GDNF may initially provide protection to some striatal terminals but that over time the protective ability of GDNF diminishes while terminals may still be degenerating. Thus, although DA cell bodies in the nigra appear to be fully protected, DA terminals in the striatum are only partially protected. Further experiments will be necessary to determine the mechanisms responsible for the decrease in GDNF protection after 10–12 weeks.

The site of GDNF administration appears to be important for determining the effectiveness of GDNF in protecting striatal DA terminals. In the present study, intranigral GDNF provided minimal long-term protection to DA terminals. Sauer et al. (1995) and Winkler et al. (1996) reported similar results after supranigral GDNF. On the other hand, the combined injection of GDNF both above the nigra and into the lateral ventricle immediately before injection of 6-OHDA into the medial forebrain bundle led to a substantial sparing of DA levels in the striatum both at 2 weeks (Opacka-Juffry et al., 1995) and 13 weeks (Sullivan et al., 1998) after lesioning. In vivo indices of DA terminal functioning (positron emission tomography scans with a DA transporter tracer and striatal microdialysis) also indicated that the combined supranigral and intraventricular injection of GDNF provided significant protection to striatal DA terminals (Opacka-Juffry et al., 1995; Sullivan et al., 1998). In addition, the intrastriatal injection of an adenoviral vector expressing GDNF provided substantial sparing of TH immunoreactivity in the striatum against the effects of intrastriatal 6-OHDA (Bilang-Bleuel et al., 1997). Injection of a similar vector into the midbrain, while reducing 6-OHDA–induced loss of DA neurons in the nigra, did not appear to spare striatal TH immunoreactivity (Choi-Lundberg et al., 1997; Mandel et al., 1997). Taken together with the present results, the evidence to date suggests that GDNF needs to be administered into the striatum, or adjacent to it, to provide significant protection to DA terminals against the effects of 6-OHDA.

In the GDNF + saline–treated animals, release and clearance of DA were not significantly altered on the GDNF-treated side compared with the contralateral side at either 3–4 or 10–12 weeks after treatment. In addition, there was no difference in release or clearance between the GDNF + saline–treated animals and the vehicle + saline–treated animals. Thus, in contrast to other reports (Hebert et al., 1996;Hebert and Gerhardt, 1997), we did not find a significant increase in evoked DA overflow in normal animals given GDNF. However, we did find that a single, intranigral injection of GDNF in normal animals produces long-term changes in tissue levels of DA. There was a significant decrease in striatal DA content at both 3–4 and 10–12 weeks after treatment but normal levels of potassium-evoked release of DA. This may indicate that DA synthesis is decreased in the striatum or that less DA is being stored in nonreleasable pools. Other investigators have also found a decrease, or a trend for a decrease, in striatal DA levels after GDNF administration (Hudson et al., 1995; Hebert et al., 1996;Martin et al., 1996).

The present result of an increase in nigral DA levels after intranigral GDNF treatment in nonlesioned animals is similar to that reported by other investigators (Gash et al., 1995; Hudson et al., 1995; Martin et al., 1996; Hebert and Gerhardt, 1997). The increase in DA content in the nigra, along with increased TH immunoreactivity in the nigra (Gash et al., 1995; Hudson et al., 1995), suggests that DA synthesis and storage may be increased in the nigra. One possible explanation for these results is that GDNF has increased DA synthesis in both the soma and terminal regions of DA neurons. In the cell bodies this leads to an accumulation of DA, whereas in the terminals there is an increase in DA turnover (Hudson et al., 1995) that leads to decreases in the stored levels of DA but to normal levels of extracellular DA. Another possible explanation for these results is that axoplasmic transport of TH to the terminals is diminished. This could lead to a buildup of TH and DA levels in the nigra and to a decrease in DA levels in the striatum. Presynaptic compensatory mechanisms may be maintaining normal extracellular levels of DA in the striatum (Zigmond et al., 1990). Further studies are needed to determine the nature and physiological significance of GDNF-induced changes in DA levels in the nigra and striatum of normal animals.

The present results suggest that a single intranigral administration of GDNF can prevent 6-OHDA–induced damage to nigral DA cell bodies. However, damage to striatal DA terminals is still present, and its extent may increase over time. It is possible that GDNF needs to be administered into the striatum, or adjacent to it in the ventricles, to afford substantial protection to DA terminals. These data further support that survival of DA cell bodies after neurotoxin lesioning does not necessarily indicate that DA terminals are functioning in their normal capacity.

Footnotes

  • This work was supported in part by the American Parkinson Disease Association. We thank Synergen (Boulder, CO) and Amgen (Thousand Oaks, CA) for the generous gift of GDNF.

    Correspondence should be addressed to Dr. Wayne A. Cass, Department of Anatomy and Neurobiology, MN 224 Chandler Medical Center, University of Kentucky, Lexington, KY 40536-0298.

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GDNF Protection against 6-OHDA–Induced Reductions in Potassium-Evoked Overflow of Striatal Dopamine
Wayne A. Cass, Michael W. Manning
Journal of Neuroscience 15 February 1999, 19 (4) 1416-1423; DOI: 10.1523/JNEUROSCI.19-04-01416.1999

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GDNF Protection against 6-OHDA–Induced Reductions in Potassium-Evoked Overflow of Striatal Dopamine
Wayne A. Cass, Michael W. Manning
Journal of Neuroscience 15 February 1999, 19 (4) 1416-1423; DOI: 10.1523/JNEUROSCI.19-04-01416.1999
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