Article Figures & Data
Figures
- Fig. 1.
cacTS2 exhibits a conditional and reversible reduction in the synaptic current. DLM synaptic currents evoked by stimulation of the DLM motor axon were recorded at 20 and 36°C, and at 20°C after exposure to 36°C (20°C recovery). The 20°C recovery traces in wild type and cacTS2 were recorded after 1 and 10 min at 20°C, respectively. Axon stimulation is marked by thearrow. In each case, the 36°C and 20°C recovery traces were obtained from the same preparation.
- Fig. 2.
The extent of synaptic current reduction incacTS2 is dependent on temperature.A, Peak amplitude measurements of DLM EPSCs fromcacTS2 are shown as a mean percentage of wild-type currents. Error bars indicate SEM, and values significantly different from wild type are marked by anasterisk. B, Sample recordings from wild type and cacTS2 at 38°C.
- Fig. 3.
The synaptic current reduction observed incacTS2 mutants maps to thecac locus. DLM synaptic current recordings incacTS2/+ indicate a recessivecacTS2 synaptic phenotype (A). Recordings fromcacTS2/Df(1)KA10(B) and cacTS2/l(1)L13 (C) map the observed synaptic phenotype to thecac locus.
- Fig. 4.
Recordings of spontaneous and evoked neurotransmitter release at ICLM synapses confirm a presynaptic role for cac-encoded α1 subunits in neurotransmitter release. A, Recordings of evoked synaptic currents from ICLM neuromuscular synapses in wild type (WT) andcacTS2. Similar results were obtained in three experiments for each genotype. B, Histograms of wild-type and cacTS2 mEPSP amplitudes (mEPP Amplitude). The solid linerepresents a Gaussian fit to the data. Insets shown representative mEPSP recordings. Similar results were obtained in three experiments from each genotype.
- Fig. 5.
cacS exhibits an activity-dependent reduction in the synaptic current. A, Sample DLM synaptic currents fromcacS in response to 1 Hz stimulation at 20 and 36°C. In each case, the first 50 traces were superimposed. Note the activity-dependent reduction in the synaptic current at both temperatures. B, Peak amplitude measurements of DLM EPSCs from wild type (WT),cacS, andcacTS2 are plotted as a function of time during 1 Hz stimulation trains at 36°C. Eachpoint represents the mean ± SEM for four experiments in cacS andcacTS2 and seven experiments in wild type.
- Fig. 6.
Heteroallelic interactions ofcacTS2 andcacS. DLM synaptic current recordings atcacTS2/cacSsynapses. The cut DLM motor axon was stimulated at 1 Hz, and in each case, the first 50 traces were superimposed. Similar results were obtained in four experiments.
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