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ARTICLE, Cellular/Molecular

The A53T α-Synuclein Mutation Increases Iron-Dependent Aggregation and Toxicity

Natalie Ostrerova-Golts, Leonard Petrucelli, John Hardy, John M. Lee, Matthew Farer and Benjamin Wolozin
Journal of Neuroscience 15 August 2000, 20 (16) 6048-6054; DOI: https://doi.org/10.1523/JNEUROSCI.20-16-06048.2000
Natalie Ostrerova-Golts
1Departments of Pharmacology and
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Leonard Petrucelli
1Departments of Pharmacology and
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John Hardy
3Department of Pharmacology, Mayo Clinic, Jacksonville, Florida 32224
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John M. Lee
1Departments of Pharmacology and
2Pathology, Loyola University Medical Center, Maywood, Illinois 60153, and
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Matthew Farer
3Department of Pharmacology, Mayo Clinic, Jacksonville, Florida 32224
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Benjamin Wolozin
1Departments of Pharmacology and
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Abstract

Parkinson's disease (PD) is the most common motor disorder affecting the elderly. PD is characterized by the formation of Lewy bodies and death of dopaminergic neurons. The mechanisms underlying PD are unknown, but the discoveries that mutations in α-synuclein can cause familial PD and that α-synuclein accumulates in Lewy bodies suggest that α-synuclein participates in the pathophysiology of PD. Using human BE-M17 neuroblastoma cells overexpressing wild-type, A53T, or A30P α-synuclein, we now show that iron and free radical generators, such as dopamine or hydrogen peroxide, stimulate the production of intracellular aggregates that contain α-synuclein and ubiquitin. The aggregates can be identified by immunocytochemistry, electron microscopy, or the histochemical stain thioflavine S. The amount of aggregation occurring in the cells is dependent on the amount of α-synuclein expressed and the type of α-synuclein expressed, with the amount of α-synuclein aggregation following a rank order of A53T > A30P > wild-type > untransfected. In addition to stimulating aggregate formation, α-synuclein also appears to induce toxicity. BE-M17 neuroblastoma cells overexpressing α-synuclein show up to a fourfold increase in vulnerability to toxicity induced by iron. The vulnerability follows the same rank order as for aggregation. These data raise the possibility that α-synuclein acts in concert with iron and dopamine to induce formation of Lewy body pathology in PD and cell death in PD.

  • Parkinson's disease
  • Lewy body
  • oxidation
  • neurodegeneration
  • ubiquitin
  • dopamine
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The Journal of Neuroscience: 20 (16)
Journal of Neuroscience
Vol. 20, Issue 16
15 Aug 2000
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The A53T α-Synuclein Mutation Increases Iron-Dependent Aggregation and Toxicity
Natalie Ostrerova-Golts, Leonard Petrucelli, John Hardy, John M. Lee, Matthew Farer, Benjamin Wolozin
Journal of Neuroscience 15 August 2000, 20 (16) 6048-6054; DOI: 10.1523/JNEUROSCI.20-16-06048.2000

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The A53T α-Synuclein Mutation Increases Iron-Dependent Aggregation and Toxicity
Natalie Ostrerova-Golts, Leonard Petrucelli, John Hardy, John M. Lee, Matthew Farer, Benjamin Wolozin
Journal of Neuroscience 15 August 2000, 20 (16) 6048-6054; DOI: 10.1523/JNEUROSCI.20-16-06048.2000
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Keywords

  • Parkinson's disease
  • Lewy body
  • oxidation
  • neurodegeneration
  • ubiquitin
  • dopamine

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