A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis

This work was supported by National Institutes of Health Grant NS29051, a grant from the Muscular Dystrophy Association, and a Marine Biological Laboratory fellowship (to E.M.L.); by National Institutes of Health Grant NS21624 and a Human Frontier Science program grant (to G.J.A.); and by Ruth K. Broad Biomedical Research Foundation and National Research Service Award fellowships (to J.R.M.). We thank M. Hale and S. Miller for performing the electron microscopy and S. Jin for performing the biosensor analysis. We also thank L. Bonewald and S. Mouton of the Institutional Protein Core Facility at University of Texas Health Science Center at San Antonio for peptide synthesis and T. Kirchhausen and E. ter Haar for the clathrin terminal domain construct and thoughtful discussions.

Correspondence should be addressed to Dr. George J. Augustine, Department of Neurobiology; Box 3209, Duke University Medical Center; Durham, NC 27710. E-mail: georgea{at}neuro.duke.edu.