Skip to main content

Umbrella menu

  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
  • ALERTS
  • FOR AUTHORS
    • Preparing a Manuscript
    • Submission Guidelines
    • Fees
    • Journal Club
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
  • SfN.org
  • eNeuro
  • The Journal of Neuroscience
  • Neuronline
  • BrainFacts.org

User menu

  • Log in
  • Subscribe
  • My alerts
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • Subscribe
  • My alerts
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
  • ALERTS
  • FOR AUTHORS
    • Preparing a Manuscript
    • Submission Guidelines
    • Fees
    • Journal Club
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
ARTICLE, Cellular/Molecular

Mechanisms of Cannabinoid Inhibition of GABAASynaptic Transmission in the Hippocampus

Alexander F. Hoffman and Carl R. Lupica
Journal of Neuroscience 1 April 2000, 20 (7) 2470-2479; DOI: https://doi.org/10.1523/JNEUROSCI.20-07-02470.2000
Alexander F. Hoffman
1Cellular Neurobiology Branch, National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carl R. Lupica
1Cellular Neurobiology Branch, National Institute on Drug Abuse, Intramural Research Program, National Institutes of Health, Baltimore, Maryland 21224
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The localization of cannabinoid (CB) receptors to GABAergic interneurons in the hippocampus indicates that CBs may modulate GABAergic function and thereby mediate some of the disruptive effects of marijuana on spatial memory and sensory processing. To investigate the possible mechanisms through which CB receptors may modulate GABAergic neurotransmission in the hippocampus, whole-cell voltage-clamp recordings were performed on CA1 pyramidal neurons in rat brain slices. Stimulus-evoked GABAA receptor-mediated IPSCs were reduced in a concentration-dependent manner by the CB receptor agonist WIN 55,212–2 (EC50 of 138 nm). This effect was blocked by the CB1 receptor antagonist SR141716A (1 μm) but not by the opioid antagonist naloxone. In contrast, evoked GABAB-mediated IPSCs were insensitive to the CB agonist. WIN 55,212–2 also reduced the frequency of spontaneous, action potential-dependent IPSCs (sIPSCs), without altering action potential-independent miniature IPSCs (mIPSCs), measured while sodium channels were blocked by tetrodotoxin (TTX). Blockade of voltage-dependent calcium channels (VDCCs) by cadmium also eliminated the effect of WIN 55,212–2 on sIPSCs. Depolarization of inhibitory terminals with elevated extracellular potassium caused a large increase in the frequency of mIPSCs that was inhibited by both cadmium and WIN 55,212–2. The presynaptic effect of WIN 55,212–2 was also investigated using the potassium channel blockers barium and 4-aminopyridine. Neither of these agents significantly altered the effect of WIN 55,212–2 on evoked IPSCs. Together, these data suggest that presynaptic CB1 receptors reduce GABAA- but not GABAB-mediated synaptic inhibition of CA1 pyramidal neurons by inhibiting VDCCs located on inhibitory nerve terminals.

  • brain slice
  • calcium channels
  • cannabis
  • electrophysiology
  • GABAA receptors
  • GABABreceptors
  • hippocampal
  • marijuana
  • potassium channels
  • presynaptic
  • ruthenium red
View Full Text
Back to top

In this issue

The Journal of Neuroscience: 20 (7)
Journal of Neuroscience
Vol. 20, Issue 7
1 Apr 2000
  • Table of Contents
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Mechanisms of Cannabinoid Inhibition of GABAASynaptic Transmission in the Hippocampus
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Mechanisms of Cannabinoid Inhibition of GABAASynaptic Transmission in the Hippocampus
Alexander F. Hoffman, Carl R. Lupica
Journal of Neuroscience 1 April 2000, 20 (7) 2470-2479; DOI: 10.1523/JNEUROSCI.20-07-02470.2000

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Mechanisms of Cannabinoid Inhibition of GABAASynaptic Transmission in the Hippocampus
Alexander F. Hoffman, Carl R. Lupica
Journal of Neuroscience 1 April 2000, 20 (7) 2470-2479; DOI: 10.1523/JNEUROSCI.20-07-02470.2000
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • brain slice
  • calcium channels
  • cannabis
  • electrophysiology
  • GABAA receptors
  • GABABreceptors
  • hippocampal
  • marijuana
  • potassium channels
  • presynaptic
  • ruthenium red

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

ARTICLE

  • Calcium Influx via L- and N-Type Calcium Channels Activates a Transient Large-Conductance Ca2+-Activated K+Current in Mouse Neocortical Pyramidal Neurons
  • Neural Correlates of Competing Fear Behaviors Evoked by an Innately Aversive Stimulus
  • Distinct Developmental Modes and Lesion-Induced Reactions of Dendrites of Two Classes of Drosophila Sensory Neurons
Show more ARTICLE

Cellular/Molecular

  • Calcium Influx via L- and N-Type Calcium Channels Activates a Transient Large-Conductance Ca2+-Activated K+Current in Mouse Neocortical Pyramidal Neurons
  • Neural Correlates of Competing Fear Behaviors Evoked by an Innately Aversive Stimulus
  • Distinct Developmental Modes and Lesion-Induced Reactions of Dendrites of Two Classes of Drosophila Sensory Neurons
Show more Cellular/Molecular
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
  • Feedback
(JNeurosci logo)
(SfN logo)

Copyright © 2021 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.