Fig. 8.
Neuroprotective effects of elevated extracellular potassium. A, Representative photomicrograph of a culture treated with elevated KCl (35 mm total) on DIV 4.B, Sister culture treated with 35 mm KCl and 3 μm DHP. C, Control culture grown with normal (5 mm) potassium. D, DHP-treated culture in normal potassium. E, F, Altered neuronal density does not explain the effect of elevated KCl. Cells were initially plated at one-third the normal density and treated with either elevated extracellular potassium alone (E) or elevated potassium and 3 μmDHP (F). Scale bars, 130 μm (bar inF applies to C–F; bar inH applies to G, H).G, Effect of KCl on muscimol toxicity.Bars represent normalized neuronal counts of cultures treated with muscimol alone (10 μm), 35 mmpotassium alone, or muscimol plus KCl (n = 4 experiments; all conditions, p < 0.05 vs control;p = 0.06 for KCl vs KCl plus muscimol conditions).
