Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE

User menu

  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
  • EDITORIAL BOARD
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
  • SUBSCRIBE
PreviousNext
ARTICLE, Development/Plasticity/Repair

The Role of Dopamine Receptors in Regulating the Size of Axonal Arbors

C. L. Parish, D. I. Finkelstein, J. Drago, E. Borrelli and M. K. Horne
Journal of Neuroscience 15 July 2001, 21 (14) 5147-5157; DOI: https://doi.org/10.1523/JNEUROSCI.21-14-05147.2001
C. L. Parish
1Neurosciences Group, Department of Medicine and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. I. Finkelstein
1Neurosciences Group, Department of Medicine and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
J. Drago
1Neurosciences Group, Department of Medicine and
2Department of Neurology, Monash Medical Center, Clayton 3168, Australia, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
E. Borrelli
3Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch Cedex, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. K. Horne
1Neurosciences Group, Department of Medicine and
2Department of Neurology, Monash Medical Center, Clayton 3168, Australia, and
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Factors that regulate terminal arbor size of substantia nigra pars compacta (SNpc) neurons during development and after injury are not well understood. This study examined the role of dopamine receptors in regulating arbor size. Terminal arbors were examined in mice with targeted deletion of the D1 or D2 dopamine receptor [D1(−/−) and D2(−/−) mice, respectively]. Terminal trees were also examined after treatment with receptor blockers and after partial SNpc lesions. Immunohistochemistry was performed, and the number of SNpc neurons and dopaminergic terminals in the striatum was estimated. The number of dopaminergic SNpc neurons were reduced in D1(−/−) and D2(−/−) mice. Density of dopaminergic terminals was unchanged in D1(−/−) mice and increased in D2 (−/−) mice. Steady-state striatal DA and DOPAC levels revealed that dopamine activity was enhanced in D2(−/−) mice but reduced in D1(−/−) mice.

Two months after partial SNpc lesions, striatal terminal density was normal in both wild-type and D1(−/−) mice but reduced in D2(−/−) mice. Administration of DA receptor antagonists resulted in larger terminal arbors in D1(−/−) and wild-type mice, whereas D2(−/−) mice showed no change in terminal density.

Functional blockade of the D2R during development or in the adult brain results in increased axonal sprouting. Partial SNpc lesions resulted in compensatory sprouting, only in mice with functional D2R. These results suggest that individual dopaminergic axons in D2(−/−) mice have reached maximal arbor size. We conclude that the D2 receptor may play a role in modulating the extent of the terminal arbor of SNpc neurons.

  • regeneration
  • dopamine receptors
  • sprouting
  • axonal arbor
  • dopamine antagonists
  • D1 receptor knock-out
  • D2 receptor knock-out
  • 6-OHDA lesions

There is now substantial evidence that neurons in the adult CNS can form new synapses, neurites, and branches (Raisman and Field, 1973; Fagan and Gage, 1994;Frotscher et al., 1997). After injury in the striatum or substantia nigra pars compacta (SNpc), a number of compensatory changes occur that suggest regenerative processes are present. These changes include the formation of new synaptic terminals, growth-cone structures (indicating axonal sprouting), neurite formation, increased number of tyrosine hydroxylase-immunoreactive (TH-IR) hypertrophic fibers penetrating the striatum, the upregulated expression of factors that support neurite outgrowth and cell survival, and increased dopamine levels (Zigmond et al., 1984; Onn et al., 1986; Hornykiewicz, 1993; Thomas et al., 1994;Blanchard et al., 1995, 1996; Cheng et al., 1998; Ho and Blum, 1998;Batchelor et al., 1999; Liberatore et al., 1999; Finkelstein et al., 2000).

Recently, we reconstructed single axons derived from SNpc neurons that survived small injections of the neurotoxin 6-hydroxydopamine (6-OHDA) (Finkelstein et al., 2000). The surviving neurons appeared to compensate for the partial denervation of the striatum by the acquisition of collateral branches and increased terminal numbers. When observed with electron microscopy, the surviving terminals were ∼30% greater in size with a morphology that suggested enhanced efficiency (Finkelstein et al., 2000). Although the remaining axons formed very large terminal arbors, up to 10 times normal size in some neurons, it appeared that the sprouting was regulated, because sprouting was proportional to the size of the lesion. For example, the density of dopamine (DA) terminals in the striatum remained at near normal levels until neuronal loss in the SNpc reached ∼80% (Finkelstein et al., 2000). This suggested to us that the extent of sprouting might be regulated to maintain normal steady-state DA levels in the striatum. If this was so, it seems likely that DA receptors, either presynaptic on nigrostriatal terminals or postsynaptic on striatal neurons, might participate in mediating the extent of the sprouting response. The predominant receptor types expressed in the dorsal tier of the striatum (the target region for the SNpc) are the D1 dopamine receptor (D1R) and D2 dopamine receptor (D2R) (Bjorklund and Lindvall, 1984; Gerfen et al., 1987; Weiner et al., 1991; Missale et al., 1998), whereas only D2 receptor transcripts are identified in nigrostriatal neurons (Drago et al., 1998). The availability of knock-out mice, with target deletion of D1R (D1 (−/−) (Drago et al., 1994) and D2R (D2 (−/−) (Baik et al., 1995), provides an opportunity for investigating the role of these two receptors in modulating the extent of sprouting in development and after partial loss of neurons in the SNpc of the adult brain. The extent of sprouting in surviving neurons after a partial lesion of the SNpc in these animals was compared with that observed in wild-type (Wt) mice. In addition the DA receptor antagonists, haloperidol andN-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ) were administered to normal and knock-out animals to further assess the role of D1R and D2R in regulating terminal arbor size of dopaminergic projection neurons. Furthermore, knowledge of the contribution of D1R and D2R in the modulation of compensatory responses may aid in the understanding and treatment of neurodegenerative disorders.

MATERIALS AND METHODS

D1 dopamine receptor-deficient mice were generated and genotyped as previously described (Drago et al., 1994). D1(+/+), heterozygous D1(+/−), and homozygous mutant D1(−/−) littermates were derived by interbreeding heterozygous mice. The heterozygous mice, originally in a hybrid C57/BL6 and 129/Svgenetic background had been backcrossed for four generations into a C57/BL6 background. D2 heterozygous mice were obtained from the original colony at Institut de Génétique et de Biologie Moléculaire et Cellulaire (Strasbourg, France). D2(+/+), D2 (+/−), and D2(−/−) littermates were derived by interbreeding D2 heterozygous mice. Again, D2 heterozygous breeders had been backcrossed for four generations on a C57/BL6 background. All mice were kept in a temperature-controlled room (22°C) under a 12 hr light/dark cycle. All mice had ad libitum access to food and water and from the time of weaning, D1(−/−) mice were also provided with vitamized rat chow supplemented with peanut butter. Southern analysis was used to identify the genotypes of all mice (Drago et al., 1994; Baik et al., 1995).

All methods conformed to the Australian National Health and Medical Research Council published code of practice for the use of animal research and were approved by the Monash University Animal Ethics Committee.

Immunohistochemistry for tyrosine hydroxylase and dopamine transporter

Animals were killed by an overdose of sodium pentobarbitone (Lethobarb; 0.35 mg/gm) and perfused with 30 ml of warmed (37°C) 0.1m PBS, pH 7.4, with heparin (1 U/ml), followed by 30 ml of chilled 4% paraformaldehyde (Sigma, St. Louis, MO) and 0.2% picric acid in 0.1 m phosphate buffer (4°C), pH 7.4. The brains were then removed and left at 4°C overnight in 30% sucrose in PBS.

A 1:15 series was cut on the coronal plane through the striatum with a section thickness of 16 μm. Sections were mounted directly onto slides coated with 0.1% chrome alum and 1% gelatin in water and then stored at −70°C until required. Coronal sections (50 μm thick) were cut through the SNpc with one series being mounted onto chrome alum gelatinized slides for counterstaining, and alternate sections were placed free-floating in cryoprotectant for TH immunohistochemistry.

Dopamine transporter immunohistochemistry. Dopamine transporter (DAT) immunohistochemistry was used to label dopaminergic terminals in the caudate putamen (CPu) so that stereological counts could be made of terminal density. The sections were fixed to the gelatinized slides with 10% neutral buffered formalin (30 sec), rinsed in PBS (3 × 10 min), then incubated in blocking solution (PBS, 0.3% Triton X-100, and 5.0% normal rabbit serum) for 15 min followed by a wash in PBS (1 × 10 min). The primary antibody rat anti-DAT (Chemicon, Temecula, CA; 1:3000 in PBS, 0.3% Triton X-100, and 1.0% normal rabbit serum) was then added to the slides and left overnight at 4°C. The next day the slides were washed in PBS (3 × 10 min), and a biotinylated secondary antibody (rabbit, anti-rat IgG, 1:300; Vector Laboratories, Burlingame, CA) was applied for 2 hr at 4°C followed by washes in PBS and incubation in avidin peroxidase for 2 hr. These sections were then washed three times in PBS (to remove unbound avidin peroxidase), incubated for 15 min in intensified cobalt–nickel diaminobenzidine (DAB) (Sigma), and finally hydrogen peroxide (0.01%) was added to this solution for a further 5 min (Adams, 1981;Finkelstein et al., 2000). The sections were then dehydrated through graded alcohol and cleared before being coverslipped using a polystyrene mounting medium.

Tyrosine hydroxylase immunohistochemistry. Tyrosine hydroxylase immunohistochemistry on free-floating sections was used to identify dopaminergic neurons within the SNpc. The 50-μm-thick sections were washed with PBS (3 × 10 min). Blocking solution was then added (PBS, 0.3% Triton X-100, and 10.0% normal goat serum) for 15 min, and the sections were then washed in PBS (10 min). The primary antibody (mouse anti-tyrosine hydroxylase; Boehringer Mannheim, Castle Hill, Australia; 1:1000 in PBS, 0.3% Triton X-100 and 1% normal goat serum) was added to the wells and left overnight at 4°C. The next day the sections were rinsed in PBS (3 × 5 min) then incubated in biotinylated secondary antibody (biotinylated goat anti-mouse, 1:300; Sigma) for 2 hr. Sections were washed in PBS and incubated in avidin peroxidase (1:5000) for 2 hr. Sections were then reacted with cobalt and nickel-intensified DAB. Sections were mounted onto slides using 1% gelatin, counterstained with 1% neutral red, dehydrated, and coverslipped.

Fractionator design for estimating total numbers of SNpc neurons and DAT-immunoreactive varicosities in CPu

The number of neurons in the SNpc and density of DAT-IR varicosities in the CPu were estimated using a fractionator sampling design (Gundersen et al., 1988; West et al., 1991; Finkelstein et al., 2000). Staining with Neutral Red delineated the area of the SNpc in each section. In each of the sections sampled, SNpc neurons were counted, using the nuclei of stained SNpc cells as the counting unit according to optical dissector rules (Gundersen et al., 1988). For TH immunohistochemistry, labeled profiles were only counted if the first recognizable profile of the cell came into focus within the counting frame (West et al., 1991). Neutral Red or tyrosine hydroxylase counts of SNpc neurons were made on alternate coronal sections. Counts were made at regular predetermined intervals (x = 140 μm, y = 140 μm). These counts were derived by means of a grid program, Stereoinvestigator (MicroBrightField, Colchester, VT), through which a systematic sample of the area occupied by the SNpc was made from a random starting point. An unbiased counting frame of known area (45 × 35 μm = 1575 μm2) was superimposed on the image of the tissue sections viewed under a 100×, numerical aperture (NA) 1.30 oil immersion objective. The entirez-dimension of each section was sampled, hence the section thickness sampling fraction was 1. Counts were taken from 10 sections at a 1:2 interval, extending from the most rostral to the most caudal parts of the SNpc. After all sections from a SNpc were analyzed, the fraction of the area of the sections sampled was calculated (West et al., 1991, 1996). The area sampling fraction is obtained by dividing the area of the counting frame by the area of the distance between sampling regions i.e., x and y intervals. As detailed above, the x and y intervals in sections were both 140 μm, and the area of the counting frame was 1575 μm2. Therefore, the area sampling fraction is 1575/(140 × 140) = 0.0804. The total number of neurons in the SNpc was estimated by multiplying the number of neurons counted within the sampled regions with the reciprocals of the fraction of the sectional area sampled and the fraction of the section thickness sampled (West et al., 1991; Coggeshall and Lekan, 1996; Finkelstein et al., 2000).

DAT-IR varicosities in the dorsal 400 μm of the CPu were counted from 16-μm-thick serial sections, at a 1 in 15 series, providing approximately eight sections from each striatum for counting. The dorsal region of CPu was chosen for sampling because it predominantly receives the SNpc projection (Fallon and Moore, 1978; Bjorklund and Lindvall, 1984; Gerfen et al., 1987), and our previous study demonstrated that sprouting was confined to this region of the striatum (Finkelstein et al., 2000). The striatum was sectioned and examined from its most rostral pole through to the level of the hippocampus heading toward the end of the triangular septal nuclei. Counts of DAT-IR varicosities were made at regular predetermined intervals (x = 170 μm, y = 170 μm). An unbiased counting frame of known area (5 × 4 μm = 20 μm2) was superimposed on the image of the tissue sections viewed under a 100×, NA 1.30 oil immersion objective. DAT-positive terminals were identified as predominantly round swellings in association with axonal processes. Total terminal numbers (DAT number) were estimated as described for counts of SNpc neurons (above). The coefficients of error (CE) and coefficients of variance (CV) were calculated as estimates of precision, and values of <0.1 were accepted (Braendgaard et al., 1990; West and Gundersen, 1990; West et al., 1991). Density of DAT-IR varicosities and the number of varicosities per neuron were calculated.

Determination of striatal dopamine levels

Dopamine levels in the dorsal striatum of D1 and D2 adult mutant mice were determined using HPLC. Mice were killed by cervical dislocation, and their brains were rapidly removed and frozen. The CPu was dissected out and weighed. The dissected striatal tissue was placed in 200 μl of 4 m perchloric acid (HClO4) containing 0.15% sodium metabisulphate (Na2S2O5) and 0.05% disodium EDTA as well as the internal standard, 0.01% dihydroxybenzylamine (DHBA).

The sample tissue was then homogenized, and cellular and vesicular membranes were disrupted using a sonicator. The samples were stored at −70°C and centrifuged on the day of analysis. The samples were analyzed by ion exchange HPLC. The HPLC was coupled to an LC-4A amphometric detector and the height of the peaks was recorded using a chart recorder with peak heights determined manually (Herges and Taylor, 1999).

The mobile phase consisted of 8% v/v methanol in purified deionized water containing 14.2 gm of trichloroacetic acid, 0.25 gm of EDTA, and 3.2 gm of sodium hydroxide. After adjusting the pH of the mobile phase to pH 2.8 (using 5 m sodium hydroxide), the final solution was filtered through a 0.45 μm Durapore membrane filter (Waters, a division of Millipore, Bedford, MA) and degassed using a vacuum pump (Herges and Taylor, 1999). For every chromatograph run, a standard curve of dopamine (0.1–2.0 μg) with DHBA as the internal standard was established, and precision, accuracy, and recovery were determined. The dopamine standards were prepared fresh before each chromatograph analysis. The internal standard (DHBA, 0.1 mg/ml) was added to each dopamine standard solution. The dopamine content of the samples was calculated from the ratios of the peak heights of dopamine to the internal standard, DHBA. The dihydroxphenylacetic acid (DOPAC) content of the sample was also calculated as the ratio of the peak height of DOPAC and the internal standard. DA activity was expressed as the concentration of DOPAC to DA, [DOPAC]/[DA].

Drug treatment groups

The reversible dopamine receptor antagonist haloperidol (2.5 mg/kg; Serenace, Searle Laboratories, Australia) was administered to animals in drinking water continuously for 2 months. The irreversible receptor antagonist EEDQ (Sigma) was dissolved in 50% ethanol then diluted with 0.9% saline to an ethanol concentration of 5%, and 6 mg/kg was injected intraperitoneally every third day for a period of 2 months.

Lesioning

A partial lesion of the SNpc was produced in the mice by injecting the neurotoxin 6-OHDA into the right SNpc. Mice were anesthetized with 4% chloral hydrate in PBS (10 ml/kg, i.p.), and heads were secured in a stereotaxic head frame with the bite bar 3 mm above horizontal. A 1.5 μg/μl solution of 6-OHDA was prepared with ascorbic acid (0.2 mg/ml) and kept on ice until the time of injection. A 10 μl Hamilton syringe (with a 26 gauge needle) mounted in a syringe pump (Cole-Parmer, Vernon Hills, IL) was inserted into the right SNpc through a small hole drilled through the top of the skull. A single injection (2.5 μg) of 6-OHDA (Sigma) was made into the right SNpc (anteroposterior, 3.0 mm; lateral, 1.05 mm; dorsoventral, 4.7 mm, with respect to lambda) (Franklin and Paxinos, 1997). On completion of the injection, the needle was left in place for 5 min then slowly withdrawn at a rate of 1 mm/min. After surgery, the skin was sutured, antiseptic (1% w/w iodine, Betadine; Faulding and Company, Salisbury, South Australia) was applied to the wound, and the mice were left in a warmed cage to recover. Paracetamol (100 mg/kg) was administered in drinking water as an analgesic after surgery.

RESULTS

SNpc neurons and DAT-positive terminals in the striatum were counted in Wt, D1(−/−), D1(+/−), D2(+/−), and D2(−/−) mice to calculate the size of the terminal arbors of SNpc neurons. Counts were also obtained on the pharmacologically manipulated as well as lesioned animals to assess their ability to regulate terminal arbor size. ANOVAs with Tukey post hoc tests were used with statistical differences set at the level of p < 0.05.

Morphology of the nigrostriatal pathway in D1 and D2 receptor knock-out mice

Stereology of the substantia nigra pars compacta

SNpc neurons were counted in Wt, D1(−/−), D1(+/−), D2(+/−) , and D2(−/−) animals (Table1). Staining with Neutral Red delineated the area of the SNpc in each section. The SNpc was recognized as the sheet of densely packed neurons of ∼16 μm in soma size. The ventral margin of the SNpc was distinguished from the substantia nigra pars reticulata neurons, the somata being larger (∼20 μm) and less densely packed than those in the SNpc. Lying rostromedially to SNpc was the ventral tegmental area, which was distinguished from the SNpc by its smaller (13 μm) and less densely packed cells. Caudally, the medial border of the SNpc abutted the medial lemniscus, which contained loosely scattered neurons (Nelson et al., 1996).

View this table:
  • View inline
  • View popup
Table 1.

Stereological estimates of SNpc neuronal counts in D1(−/−) and D2(−/−) mice

No significant difference was seen in the number of SNpc neurons in the two groups of Wt mice (D1+/+ and D2+/+) hence, the results from these mice were pooled in subsequent analyses. In both D1 and D2 receptor knock-out mice, the number of neurons in the SNpc was significantly less (19 and 22%, respectively) than in the Wt controls (Table 1, Figs. 1A,2B.) The CE for SNpc counts for these animals ranged from 0.018 to 0.097 and a CV was between 0.02 and 0.89, indicating an accurate sampling protocol. In these same animals, tyrosine hydroxylase-positive SNpc neurons were counted in alternate sections to those in which Neutral Red SNpc cells were counted. As expected, the majority (90%) of neurons in the SNpc of Wt mice were TH-IR (Table 1). In both D1 and D2 receptor knock-out mice however, the proportion of TH-IR neurons was significantly reduced (64 and 77% of total SNpc neurons respectively) (Table 1, Figs. 1, 2). Counts of Neutral Red and tyrosine hydroxylase-stained SNpc neurons in heterozygous mice were intermediate between those of the Wt and receptor knock-out mice (Fig. 1, Table 1).

Fig. 1.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 1.

Histograms showing the number of neurons counted in the SNpc (mean ± SD) of D1 dopamine receptor mutants (A) and D2 dopamine receptor mutants (B). Counts of SNpc neurons stained with Neutral Red are shown in black, and TH-IR counts are shown ingray. A, D1(−/−) have significantly fewer (19%) Neutral Red-stained cells and 43% fewer TH-IR cells than Wt mice. Counts in heterozygous mice were intermediate between Wt and D1(−/−) and significantly different to both. B, Similarly, counts of Neutral Red-stained cells and TH-IR cells are reduced (23 and 36%, respectively) in D2(−/−) compared with Wt and with heterozygous counts intermediate between those seen in the D2(−/−) and Wt.

Fig. 2.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 2.

A–C are photomicrographs showing Neutral Red staining through the middle to rostral portion of SNpc in Wt, D1(−/−), and D2(−/−) mice, respectively. D–Fare photomicrographs of TH-IR SNpc neurons. Note the significant reduction in the counts of SNpc neurons in the receptor knock-out mice compared with Wt. Scale bars, 250 μm.

Stereology of DAT-labeled varicosities

Striatal tissue was processed for DAT immunohistochemistry, and counts of varicosities were estimated stereologically as described in Materials and Methods (see Fig. 5). Density was determined as the number of varicosities estimated within the chosen counting area. DAT-IR varicosities and terminals were uniformly distributed within the dorsal striatum. The density of varicosities in the CPu of D1(−/−) mice was normal, whereas in D2(−/−) mice it was significantly greater than in Wt and D1(−/−) animals (74% increase) (Fig.3, Table 1).

Fig. 3.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 3.

Histograms of estimates of DAT-IR terminal density in the dorsal CPu of D1(−/−) mice (A) and D2(−/−) mice (B). A, There is no significant difference between terminal densities of Wt, D(+/−), and D1(−/−)mice. B, The density of terminals in the dorsal CPu of D2(−/−) is significantly greater than in Wt mice, with heterozygous animals having intermediate densities.

We wished to derive a representation of the average size of the terminal arbor of SNpc neurons for each genotype. The only way to measure the actual size of the arbor is to anterogradely fill and reconstruct individual axons, which is time-consuming and labor-intensive. Because of these constraints only a small sample of the neuronal population can be analyzed. Ideally, arbor size could be obtained by dividing the total number of DAT-IR terminals in the dorsal CPu (obtained by multiplying DAT-IR density by volume of dorsal CPu) by the actual number of TH-IR neurons counted in the SNpc. However, the precise volume of the dorsal tier innervated by SNpc neurons cannot be delineated and furthermore, the volume of the CPu varies according to the genotype. Previously we have devised a method for comparing arbor size after different treatments by dividing terminal density by the number of SNpc neurons (Finkelstein et al., 2000). However, this method assumes that the volume of the CPu volume and hence the volume of the dorsal tier innervated by SNpc neurons is the same in all groups. Because the volume of the CPu in the D1(−/−) mice is significantly less than D2(−/−) and Wt mice (Table 1), this method is not suitable for this study. We therefore devised a new method for determining arbor size, which takes into consideration varying striatal volumes. We can estimate the total volume of the CPu (VCPu), and we have assumed that the proportion of the CPu volume innervated by SNpc neurons is the same in all genotypes. Dividing VCPu by the number of SNpc provides a figure that is proportional to the average size of the terminal tree of an individual SNpc neuron in a particular genotype and provides a means for comparing the extent of branching across genotypes. Because this number was obtained from the whole volume of the CPu (rather than the dorsal tier), it is proportional to rather than a precise indication of the size of the terminal arbor and may result in an overestimation of the terminal tree size. First, we obtained a normalized representation of the number of terminals (NTN) in the dorsal CPu (Fig.4) using the formula: Embedded Imagewhere DT = density of terminals in the dorsal CPu, and subscript refers to the relevant genotype. In Figure 4 this value was plotted against the number of TH-IR SNpc neurons in each animal. However, the size of the average terminal tree can be determined by dividing the terminal number by the number of SNpc. Hence, terminal tree size (TT) is the number of DAT terminals (DT) multiplied by CPu volume (VCPu) divided by the number of SNpc neurons (NSNpc), for any given genotype neurons (Tables 1, 2). Embedded ImageFor the purpose of comparison a normalized terminal tree (NTT) was calculated using the terminal tree size of Wt as the standard: Embedded ImageAlthough the density of varicosities in the CPu of D1(−/−) mice was normal, the number of TH-IR SNpc neurons and volume of CPu was reduced (Fig. 1) so that the NTT of SNpc neurons in the D1(−/−) animals was in fact, larger than in the Wt mice (Table1).

Fig. 4.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 4.

A plot of normalized number of DAT-IR terminals in the dorsal striatum plotted against number of neurons in the SNpc. The number of DAT terminals in the dorsal striatum of D1(−/−) mice (open circles) was approximately half of that observed in Wts (filled circles and open triangles). However, D1(−/−) mice had reduced numbers of SNpc neurons and consequently, larger terminal trees (Table 1). In the case of D2(−/−) mice (closed triangle), the normalized terminal number in the dorsal striatum was three times normal despite a significant reduction in the number of SNpc neurons.

View this table:
  • View inline
  • View popup
Table 2.

Stereological estimates of SNpc neuronal counts in D1(−/−) and D2(−/−) mice

The density of varicosities in the CPu of D2(−/−) mice was significantly greater than in Wt and D1(−/−) animals (74% increase) (Figs. 3, 5, Table 1). Because of this (and the reduced number of TH-IR SNpc neurons in D2(−/−) mice), the NTT of TH-IR SNpc neurons in the D2(−/−) mice were ∼300% larger than in the Wt controls. The counts from D2(+/−) mice were intermediate between those of the Wt and D2(−/−) mice.

Fig. 5.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 5.

Photomicrographs of DAT-IR varicosities in the dorsal CPu of Wt, D1(−/−), and D2(−/−) mice (A–C, respectively). Note the similarity in density of DAT-IR in Wt and D1(−/−) mice, whereas the increased density of DAT-IR varicosities in the D2(−/−) mice. Dshows a diagram of the counting frame seen in B. Here, five varicosities were counted in total and include thegray varicosities within the frame as well as theblack varicosities that lie on the inclusion lines. Thewhite varicosity was not counted because it lay on an exclusion line. Scale bar, 25 μm.

Determination of striatal dopamine activity

Basal levels of dopamine and DOPAC in the dorsal striatum of Wt, D1(+/−), D1(−/−), D2(+/−), and D2(−/−) mice were determined and expressed as amount of dopamine or DOPAC per gram of CPu tissue and dopamine activity, expressed as the ratio of DOPAC per dopamine. Although dopamine levels in the striatum of D2(−/−), D2(+/−), and D1(+/−) (n = 6, 7, and 11, respectively) were not significantly different from controls (n = 19) (Fig.6A,B), dopamine levels in D1(−/−) mice (n = 6) were significantly greater (33%) than in Wt mice. However, both DOPAC levels and dopamine activity were significantly reduced in the D1(−/−) mice (n = 6) compared with wild-types (n = 12), suggesting increased dopamine storage and decreased turnover in the dopaminergic terminals of these animals (Fig.6A–C).

Fig. 6.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 6.

Dopamine and DOPAC concentrations (mean ± SD) in the CPu of D1(−/−) mice and D2(−/−) mice (A,B, respectively). Dopamine concentration is significantly greater in D1(−/−) mice than in wild types, whereas there was no difference between dopamine concentration in D2(−/−) mice and Wts. DOPAC levels were decreased in D1(−/−) mice, whereas DOPAC levels were elevated in D2(−/−) (B). Dopamine activity was reduced in the D1(−/−) and increased in the D2(−/−) mice (C). D shows a plot of the level of dopamine activity per SNpc terminal in the striatum of Wt, D1(−/−), and D2(−/−) mice (calculated by dividing DA activity by terminal density). In the D1(−/−) mice, dopamine activity per terminal were reduced, whereas they were significantly increased in D2(−/−) mice.

Conversely, dopamine levels were normal in the D2 (−/−) mice, whereas there was a statistically significant increase in DOPAC levels and dopamine activity (n = 3). Together, this implies normal dopamine storage but a high dopamine turnover (Fig. 6).

Dopamine activity per terminal was calculated by dividing each measure by the density of DAT-IR terminals. Although D2(−/−) mice had normal striatal dopamine levels, dopamine activity per terminal was markedly increased (Fig. 6D). In contrast, striatal dopamine levels in D1(−/−) mice were elevated, yet dopamine activity was sixfold less than Wt mice (Fig.6D).

Effects of dopamine receptor antagonists on D1 and D2 receptor knock-out mice

In haloperidol-treated Wt mice, DAT terminal density was 34% greater than in untreated Wt mice and 37% greater in EEDQ-treated mice than untreated Wt mice (Table 2). Terminal density also increased in D1(−/−) mice after treatment with haloperidol (46%) and EEDQ (55%) (Table 2). In contrast, there was no significant change in terminal density of D2(−/−) mice after treatment with either antagonist (Fig.7).

Effects of 6-OHDA lesioning of the SNpc in D1 and D2 receptor knock-out mice

A small dose of the neurotoxin 6-OHDA was injected into the right SNpc of Wt, D1(−/−), and D2(−/−) mice to produce a partial lesion. Animals were allowed to recover over 2 months, and the number of SNpc neurons as well as DAT-IR varicosities in the dorsal CPu were counted and density was calculated. An index of the terminal tree (TT) size was made (as above), and an NTT was calculated. In total, 17 Wt (+/+), 14 D1(−/−), and 13 D2(−/−) mice received partial lesions of their right SNpc. Stereological estimates of SNpc neuron numbers confirmed that a variety of lesion sizes were created, ranging from 2 to 83%. In some animals, the number of neurons in the contralateral SNpc was reduced, presumably because of diffusion of the toxin. In these cases, the contralateral hemisphere was included as an example of a small lesion size.

As shown previously in rats (Finkelstein et al., 2000), our estimates of DAT-IR terminal density showed that Wt mice were able to maintain terminal density within a normal range until >75% of SNpc neurons were lost. D1 (−/−) mice also maintained normal terminal density in the CPu until ∼75% of SNpc neurons was destroyed (Figs.8A,9B). In contrast, in D2(−/−) mice, terminal density was reduced linearly in proportion to the lesion size. After a 40% lesion, terminal density fell by ∼50% of nonlesioned values in D2(−/−) mice and by ∼100% after lesions of ≥75% (Figs. 8B, 9C). After lesions of wild-type and D1(−/−) mice, there was no change in distribution of terminals or density of terminals. However, there was clear reduction in density of terminals in the dorsal striatum after lesions of the D2(−/−), compared with ventral striatum. In all genotypes, terminals were again predominantly found in the matrix region innervated by the dorsal SNpc. In the lesioned animals, the SNpc cell loss was more pronounced around the site of the injection. There was, however, no visible unevenness of terminals within the dorsal striatum of Wt or D1 mice. After larger lesions in D2(−/−), terminal density was unevenly distributed with areas showing no terminals, whereas other regions contained small clusters of varicosities.

Fig. 7.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 7.

Histograms of density of DAT-IR terminals (mean ± SD) in the dorsal CPu of Wt, D1(−/−) and D2(−/−) mice, treated with haloperidol and EEDQ. There was no significant difference in density of terminals in untreated Wts and D1(−/−) mice however, treatment with either antagonist resulted in significantly increased density of terminals in both types of mice. Density of terminals in untreated D2(−/−) mice was higher than in treated Wts and D1(−/−) mice yet, treatment with either haloperidol or EEDQ had no significant effect on the terminal densities seen in the D2 (−/−) mice.

Fig. 8.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 8.

Plots of density of DAT-IR terminals in the CPu against the size of SNpc lesion.A, Density of DAT-IR terminals in Wt is maintained until ∼75% SNpc neurons are lost, at which point terminal density rapidly falls, presumably because remaining neurons can no longer compensate through sprouting. Similarly in D1(−/−) mice (B), density of DAT-IR terminals remains normal until the lesion size approaches 75%, at which point density falls. In contrast, density of DAT-IR terminals in D2(−/−) mice (C) is abnormally high in the absence of a lesion and falls linearly, in proportion to the neurons lost. In all three figures, triangles represent values from unlesioned animals, and circles are representative of the lesioned animals.

Fig. 9.
  • Download figure
  • Open in new tab
  • Download powerpoint
Fig. 9.

Photomicrograph showing examples of the density of DAT-IR terminals in animals with ∼70% lesions of the SNpc from Wt (A), D1(−/−) (B), and D2(−/−) (C) mice. Note the reduced density of DAT-IR terminals in a 70% lesioned D2 animal.

DISCUSSION

This study demonstrates the role of the D1R and D2R in modulating the size of the striatal terminal arbor of SNpc neurons and in regulating steady-state striatal DA levels.

In both D1(−/−) and D2(−/−) mice the number of SNpc neurons was reduced. Nevertheless, in both genotypes the size of the terminal tree of SNpc neurons is greater than in Wt mice, the increase being more pronounced in D2(−/−). The increase in terminal arbor size in D2(−/−) mice and increased DA activity is consistent with the hypothesis that the D2 autoreceptor acts to regulate the delivery of DA. Our findings suggest that this regulation is not confined to DA storage, synthesis, and turnover in the terminals but is also manifest in the density of DA terminals.

Released dopamine is converted to DOPAC after reuptake into the nerve terminal and into homovanillic acid at extraneuronal sites. DOPAC however, is the major dopamine metabolite in rodent brains and, levels in the striatum reflect the activity of dopaminergic neurones within the nigrostriatal pathway (Cooper et al., 1996). Dopamine activity, expressed as a ratio of DOPAC and dopamine is independent of tissue weight, thereby yielding a reliable index. Using these measures, dopamine activity in the D2(−/−) mice was high, despite normal dopamine levels. This suggests impaired regulation of dopamine storage and release, as might be expected when D2 autoreceptor function is not present (Cooper et al., 1996). In contrast, D1(−/−) mice have high levels of dopamine but low dopamine activity, suggesting that in these animals dopamine levels reflect storage within terminals with reduced transmitter release. This suggests augmented D2 function in D1 mutants. A direct mechanism seems unlikely, because the D1 receptor is not expressed presynaptically. However, a compensatory increase in D2 levels seen in the D1(−/−) mice (data not shown) may underlie the augmented D2 function. Either transynaptic signaling or some “long loop” signaling through striatal projections onto SNpc cell bodies may also contribute.

In a previous study in rats we reconstructed individual axons and demonstrated that after lesions, axonal arbors were larger (Finkelstein et al., 2000). This increase was related to the extent of the lesion. In that study we developed the method for estimating neuronal arbor size by estimating terminal density and dividing this by the number of SNpc neurons. This was found to correlate well with reconstructed arbor size, and a similar method has been used in this current study. In that study, striatal volumes were the same in each animal, reducing the assumptions and arithmetic corrections necessary in the present study. Nevertheless, the concordance between the two studies is reassuring. In rats we found that terminal density was maintained within the normal range until the number of SNpc neurons were reduced by ∼80%. We confirmed this observation in Wt and D1(−/−) mice. In both groups, terminal density was normal until ∼75% of SNpc neurons were destroyed. The size of the terminal arbors must have progressively increased in both Wt and D1(−/−) mice, until lesions reached ∼75%, beyond this point remaining neurons could compensate no further, hence the decreased density. The findings were quite different in the D2(−/−) mice. Terminal density was abnormally high in these animals and did not increase, even after EEDQ and haloperidol, which increased terminal density in Wt and D1(−/−) mice. After lesions in D2(−/−) mice, terminal density progressively fell in proportion to the size of the lesion, suggesting that the size of the terminal arbor did not increase to compensate for the effect of the lesion.

To explain these findings, we conclude that the D2R must play a role in regulating terminal density. In Wt and D1(−/−) mice, terminal density is at normal levels, and blockade of D2R results in an increase in terminal density and size of terminal arbor. The lack of effect of haloperidol and EEDQ on D2(−/−) mice suggests that when affecting terminal tree size, these agents are acting through the D2R rather than other DA receptors. The results also suggest that during development, lack of D2R results in unchecked branching of DA terminals. Further stimulus to sprout through lesioning cannot elicit a compensatory response, presumably because maximal sprouting has already occurred in these animals. The extensive sprouting of SNpc neurons in the striatum of D2(−/−) mice is not readily explained by the neurotrophic hypothesis, because glial cell-derived neurotrophic factor levels are reduced, and levels of brain derived neurotrophic factor and neurotrophin-3 are unchanged in their striatum (Bozzi and Borrelli, 1999).

The dopamine D2 receptor exists in two forms; as an autoreceptor present on the presynaptic cell and as a postsynaptic receptor (Creese, 1982; Kandel et al., 1991; Usiello et al., 2000). Because D2(−/−) mice used in this study have both isoforms ablated, the identity of the specific D2R involved in the regulation of terminal arbor size and regenerative sprouting remains unknown. However, because the presynaptic receptor is expressed at a higher level and has a role in regulating the firing rate and propagation of action potentials as well as DA synthesis and release (Cooper et al., 1996), we hypothesize that this receptor is most likely to be the major player regulating proliferation and sprouting in SNpc neurons. Recently it was reported that D2 agonists may delay and reduce dyskinesia in Parkinson's disease (Rascol et al., 2000). It is of interest to speculate whether use of D2 agonists in Parkinson's disease may result in reduced compensatory sprouting and hence reduced dyskinesia but at the cost of increased severity of the disease because of reduction in regenerative sprouting mechanism.

Although striatal dopamine levels in D1(−/−) mice were elevated, as previously described (El-Ghundi et al., 1998), dopamine activity was markedly reduced. This suggests that the behavior of D1(−/−) mice is not explained by enhanced dopaminergic activity but by some other mechanism. On the other hand, despite our finding of increased dopamine activity in D2(−/−) mice, their behavior is normal in most respects, suggesting that postsynaptic compensation has occurred (Clifford et al., 2000). It must be stressed however that DA measures in this study are not synaptic DA but the levels of total DA (including extracellular DA and vesicular DA) and DOPAC. Although these are useful markers of DA storage and turnover, a more detailed examination of synaptic function is required before drawing firm conclusions about the synaptic effects of DA in these mutants. Nevertheless, it is widely held that the presynaptic D2R regulates DA synthesis and release (Cooper et al., 1996), and it seems likely that absence of its influence has resulted in increased DA activity and sprouting in the D2(−/−) mutants. These studies were conducted on genetically manipulated mice and, because D2Rs are normally expressed early in development (Drago et al., 1998), it is difficult to ignore potential developmental compensatory effects. These effects may have been reflected in both the absolute number and the proportion of TH-IR-positive nigral neurons as well as the density of DAT-IR terminals in the striatum of the D1(−/−) and D2(−/−) mice.

The reason for the reduction in the number of SNpc neurons and TH-IR SNpc neurons is not clear. Widespread changes are recognized in the dopamine receptor knock-out mice (Drago et al., 1998). Transynaptic effects, in addition to dysregulated supply of factors known to be important for survival of neurons (Bozzi and Borrelli, 1999), may be important. Furthermore, chronic DA receptor stimulation and blockade may also have effects on both TH and l-aromatic amino acid decarboxylase enzyme activity and mRNA levels (Cho et al., 1997). The presynaptic location of the D2R and the direct input of the D1R-positive terminals on the TH-positive elements (Caille et al., 1996) provide further potential mechanisms for the modulation of the growth of nigrostriatal cells and the effect of transcriptional regulation. On the basis of the studies on receptor knock-out mice alone, it would be hard to assess whether the increased terminal tree was in response to reduced cell numbers or disturbances in regulation of axonal proliferation. However, the responses to receptor antagonists (haloperidol and EEDQ) and 6-OHDA lesions are clearly a response of the adult system that can be explained in the way that we have offered. Furthermore, the wild-type mice demonstrated these responses, indicating that they are generic responses to injury shared by both genetically manipulated and normal animals.

One of the reasons for using genetically manipulated animals is the absence of a pure DA receptor antagonist. Although haloperidol has a greater effect on D2R, it also acts at all DA receptors as well as other receptor systems, including the acetylcholine receptor, and some of its effects could have been mediated by actions other than through DA receptors. However, the actions of EEDQ are relatively DA receptor-specific and were similar to those of haloperidol, suggesting that this was unlikely.

There appears to be a maximum size of terminal tree, as seen in the D2 (−/−) mice and in the Wt and D1(−/−) after lesions of ∼75%. This suggests that an individual neuron can support a maximum number of terminals, and it is interesting to speculate regarding what factors might limit further expansion. These may include limitations caused by metabolic demand, oxidative stress, and axonal transport.

In summary, these findings suggest that the D2 receptor may play a role in modulating the extent of the terminal arbor of SNpc neurons. These results require further elaboration with the use of selective D2 receptor agonists and antagonists and the use of D2 isotype-specific mutants as well as investigations into the paths through which these influences are exerted.

Footnotes

  • This work was supported by grants from the Bethlehem Griffith Foundation and the Australian National Health and Medical Research Council. J.D. is a Logan Fellow at Monash University. We thank J. Marriott (Pharmacy College, Monash University) for establishing HPLC in the laboratory and J. S. Massalas (Department of Medicine, Monash University) for technical assistance.

    C.L.P. and D.I.F. contributed equally to this work.

    Correspondence should be addressed to Prof. Malcolm Horne, Department of Neurology, Monash Medical Centre, Clayton Road, Clayton, 3168, Australia. E-mail: malcolm.horne{at}med.monash.edu.au.

REFERENCES

  1. ↵
    1. Adams JC
    (1981) Heavy metal intensification of DAB-based HRP reaction product. J Histochem Cytochem 29:775.
    OpenUrlCrossRefPubMed
  2. ↵
    1. Baik JH,
    2. Picetti R,
    3. Saiardi A,
    4. Thiriet G,
    5. Dierich A,
    6. Depaulis A,
    7. Le Meur M,
    8. Borrelli E
    (1995) Parkinsonian-like locomotor impairment in mice lacking dopamine D2 receptors. Nature 377:424–428.
    OpenUrlCrossRefPubMed
  3. ↵
    1. Batchelor PE,
    2. Liberatore GT,
    3. Wong JY,
    4. Porritt MJ,
    5. Frerichs F,
    6. Donnan GA,
    7. Howells DW
    (1999) Activated macrophages and microglia induce dopaminergic sprouting in the injured striatum and express brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. J Neurosci 19:1708–1716.
    OpenUrlAbstract/FREE Full Text
  4. ↵
    1. Bjorklund A,
    2. Lindvall O
    (1984) Dopamine-containing systems in the CNS. (Elsevier, Amsterdam).
  5. ↵
    1. Blanchard V,
    2. Chritin M,
    3. Vyas S,
    4. Savasta M,
    5. Feuerstein C,
    6. Agid Y,
    7. Javoy-Agid F,
    8. Raisman-Vozari R
    (1995) Long-term induction of tyrosine hydroxylase expression: compensatory response to partial degeneration of the dopaminergic nigrostriatal system in the rat brain. J Neurochem 64:1669–1679.
    OpenUrlPubMed
  6. ↵
    1. Blanchard V,
    2. Anglade P,
    3. Dziewczapolski G,
    4. Savasta M,
    5. Agid Y,
    6. Raisman-Vozari R
    (1996) Dopaminergic sprouting in the rat striatum after partial lesion of the substantia nigra. Brain Res 709:319–325.
    OpenUrlCrossRefPubMed
  7. ↵
    1. Bozzi Y,
    2. Borrelli E
    (1999) Absence of the dopamine D2 receptor leads to a decreased expression of GDNF and NT-4 mRNAs in restricted brain areas. Eur J Neurosci 11:1275–1284.
    OpenUrlCrossRefPubMed
  8. ↵
    1. Braendgaard H,
    2. Evans SM,
    3. Howard CV,
    4. Gundersen HJ
    (1990) The total number of neurons in the human neocortex unbiasedly estimated using optical disectors. J Microsc 157:285–304.
    OpenUrlCrossRefPubMed
  9. ↵
    1. Caille I,
    2. Dumartin B,
    3. Bloch B
    (1996) Ultrastructural localization of D1 dopamine receptor immunoreactivity in rat striatonigral neurons and its relation with dopaminergic innervation. Brain Res 730:17–31.
    OpenUrlCrossRefPubMed
  10. ↵
    1. Cheng HW,
    2. Tong J,
    3. McNeill TH
    (1998) Lesion-induced axon sprouting in the deafferented striatum of adult rat. Neurosci Lett 242:69–72.
    OpenUrlCrossRefPubMed
  11. ↵
    1. Cho S,
    2. Neff NH,
    3. Hadjiconstantinou M
    (1997) Regulation of tyrosine hydroxylase and aromatic l-amino acid decarboxylase by dopaminergic drugs. Eur J Pharmacol 323:149–157.
    OpenUrlCrossRefPubMed
  12. ↵
    1. Clifford JJ,
    2. Usiello A,
    3. Vallone D,
    4. Kinsella A,
    5. Borrelli E,
    6. Waddington JL
    (2000) Topographical evaluation of behavioural phenotype in a line of mice with targeted gene deletion of the D2 dopamine receptor. Neuropharmacology 39:382–390.
    OpenUrlCrossRefPubMed
  13. ↵
    1. Coggeshall RE,
    2. Lekan HA
    Methods for determining numbers of cells and synapses: a case for more uniform standards of review. J Comp Neurol 364 1996 6 15 [Erratum (1996) 369:162]
    OpenUrlCrossRefPubMed
  14. ↵
    1. Cooper JR,
    2. Bloom FE,
    3. Roth RH
    (1996) The biochemical basis of neuropharmacology, Ed 7. (Oxford UP, New York).
  15. ↵
    1. Creese
    (1982) Dopamine receptors explained. Trends Neurosci 5:40–43.
    OpenUrl
  16. ↵
    1. Drago J,
    2. Gerfen CR,
    3. Lachowicz JE,
    4. Steiner H,
    5. Hollon TR,
    6. Love PE,
    7. Ooi GT,
    8. Grinberg A,
    9. Lee EJ,
    10. Huang SP,
    11. Bartlett PF,
    12. Jose PA,
    13. Sibley DR,
    14. Westphal H
    (1994) Altered striatal function in a mutant mouse lacking D1A dopamine receptors. Proc Natl Acad Sci USA 91:12564–12568.
    OpenUrlAbstract/FREE Full Text
  17. ↵
    1. Drago J,
    2. Padungchaichot P,
    3. Accili D,
    4. Fuchs S
    (1998) Dopamine receptors and dopamine transporter in brain function and addictive behaviors: insights from targeted mouse mutants. Dev Neurosci 20:188–203.
    OpenUrlCrossRefPubMed
  18. ↵
    1. El-Ghundi M,
    2. George SR,
    3. Drago J,
    4. Fletcher PJ,
    5. Fan T,
    6. Nguyen T,
    7. Liu C,
    8. Sibley DR,
    9. Westphal H,
    10. O'Dowd BF
    (1998) Disruption of dopamine D1 receptor gene expression attenuates alcohol-seeking behavior. Eur J Pharmacol 353:149–158.
    OpenUrlCrossRefPubMed
  19. ↵
    1. Fagan AM,
    2. Gage FH
    (1994) Mechanisms of sprouting in the adult central nervous system: cellular responses in areas of terminal degeneration and reinnervation in the rat hippocampus. Neuroscience 58:705–725.
    OpenUrlCrossRefPubMed
  20. ↵
    1. Fallon JH,
    2. Moore RY
    (1978) Catecholamine innervation of the basal forebrain. IV. Topography of the dopamine projection to the basal forebrain and neostriatum. J Comp Neurol 180:545–580.
    OpenUrlCrossRefPubMed
  21. ↵
    1. Finkelstein DI,
    2. Stanic D,
    3. Parish CL,
    4. Tomas D,
    5. Dickson K,
    6. Horne MK
    (2000) Axonal sprouting following lesions of the rat substantia nigra. Neuroscience 97:99–112.
    OpenUrlCrossRefPubMed
  22. ↵
    1. Franklin KBJ,
    2. Paxinos G
    (1997) The mouse brain in stereotaxic coordinates. (Academic, San Diego).
  23. ↵
    1. Frotscher M,
    2. Heimrich B,
    3. Deller T
    (1997) Sprouting in the hippocampus is layer-specific. Trends Neurosci 20:218–223.
    OpenUrlCrossRefPubMed
  24. ↵
    1. Gerfen CR,
    2. Herkenham M,
    3. Thibault J
    (1987) The neostriatal mosaic: II. Patch- and matrix-directed mesostriatal dopaminergic and non-dopaminergic systems. J Neurosci 7:3915–3934.
    OpenUrlAbstract/FREE Full Text
  25. ↵
    1. Gundersen HJ,
    2. Bagger P,
    3. Bendtsen TF,
    4. Evans SM,
    5. Korbo L,
    6. Marcussen N,
    7. Moller A,
    8. Nielsen K,
    9. Nyengaard JR,
    10. Pakkenberg B,
    11. Sørensen FB,
    12. Vesterby A,
    13. West MJ
    (1988) The new stereological tools: disector, fractionator, nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS 96:857–881.
    OpenUrlCrossRefPubMed
  26. ↵
    1. Herges S,
    2. Taylor DA
    (1999) Modulatory effect of p-chlorophenylalanine microinjected into the dorsal and median raphe nuclei on cocaine-induced behaviour in the rat. Eur J Pharmacol 374:329–340.
    OpenUrlCrossRefPubMed
  27. ↵
    1. Ho A,
    2. Blum M
    (1998) Induction of interleukin-1 associated with compensatory dopaminergic sprouting in the denervated striatum of young mice: model of aging and neurodegenerative disease. J Neurosci 18:5614–5629.
    OpenUrlAbstract/FREE Full Text
  28. ↵
    1. Hornykiewicz O
    (1993) Parkinson's disease and the adaptive capacity of the nigrostriatal dopamine system: possible neurochemical mechanisms. Adv Neurol 60:140–147.
    OpenUrlPubMed
  29. ↵
    1. Kandel ER,
    2. Schwartz JH,
    3. Jessell TM
    (1991) Principles of neural science, Ed 3. (Elsevier, New York).
  30. ↵
    1. Liberatore GT,
    2. Finkelstein DI,
    3. Wong JY,
    4. Horne MK,
    5. Porritt MJ,
    6. Donnan GA,
    7. Howells DW
    (1999) Sprouting of dopaminergic axons after striatal injury: confirmation by markers not dependent on dopamine metabolism. Exp Neurol 159:565–573.
    OpenUrlCrossRefPubMed
  31. ↵
    1. Missale C,
    2. Nash SR,
    3. Robinson SW,
    4. Jaber M,
    5. Caron MG
    (1998) Dopamine receptors: from structure to function. Physiol Rev 78:189–225.
    OpenUrlCrossRefPubMed
  32. ↵
    1. Nelson EL,
    2. Liang CL,
    3. Sinton CM,
    4. German DC
    (1996) Midbrain dopaminergic neurons in the mouse: computer-assisted mapping. J Comp Neurol 369:361–371.
    OpenUrlCrossRefPubMed
  33. ↵
    1. Onn SP,
    2. Berger TW,
    3. Stricker EM,
    4. Zigmond MJ
    (1986) Effects of intraventricular 6-hydroxydopamine on the dopaminergic innervation of striatum: histochemical and neurochemical analysis. Brain Res 376:8–19.
    OpenUrlCrossRefPubMed
  34. ↵
    1. Raisman G,
    2. Field PM
    (1973) A quantitative investigation of the development of collateral reinnervation after partial deafferentation of the septal nuclei. Brain Res 50:241–264.
    OpenUrlCrossRefPubMed
  35. ↵
    1. Rascol O,
    2. Brooks DJ,
    3. Korczyn AD,
    4. De Deyn PP,
    5. Clarke CE,
    6. Lang AE
    (2000) A five-year study of the incidence of dyskinesia in patients with early Parkinson's disease who were treated with ropinirole or levodopa. 056 Study Group. N Engl J Med 342:1484–1491.
    OpenUrlCrossRefPubMed
  36. ↵
    1. Thomas J,
    2. Wang J,
    3. Takubo H,
    4. Sheng J,
    5. de Jesus S,
    6. Bankiewicz KS
    (1994) A 6-hydroxydopamine-induced selective parkinsonian rat model: further biochemical and behavioral characterization. Exp Neurol 126:159–167.
    OpenUrlCrossRefPubMed
  37. ↵
    1. Usiello A,
    2. Baik JH,
    3. Rouge-Pont F,
    4. Picetti R,
    5. Dierich A,
    6. LeMeur M,
    7. Piazza PV,
    8. Borrelli E
    (2000) Distinct functions of the two isoforms of dopamine D2 receptors. Nature 408:199–203.
    OpenUrlCrossRefPubMed
  38. ↵
    1. Weiner DM,
    2. Levey AI,
    3. Sunahara RK,
    4. Niznik HB,
    5. O'Dowd BF,
    6. Seeman P,
    7. Brann MR
    (1991) D1 and D2 dopamine receptor mRNA in rat brain. Proc Natl Acad Sci USA 88:1859–1863.
    OpenUrlAbstract/FREE Full Text
  39. ↵
    1. West MJ,
    2. Gundersen HJ
    (1990) Unbiased stereological estimation of the number of neurons in the human hippocampus. J Comp Neurol 296:1–22.
    OpenUrlCrossRefPubMed
  40. ↵
    1. West MJ,
    2. Slomianka L,
    3. Gundersen HJ
    (1991) Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat Rec 231:482–497.
    OpenUrlCrossRefPubMed
  41. ↵
    1. West MJ,
    2. Ostergaard K,
    3. Andreassen OA,
    4. Finsen B
    (1996) Estimation of the number of somatostatin neurons in the striatum: an in situ hybridization study using the optical fractionator method. J Comp Neurol 370:11–22.
    OpenUrlCrossRefPubMed
  42. ↵
    1. Zigmond MJ,
    2. Acheson AL,
    3. Stachowiak MK,
    4. Stricker EM
    (1984) Neurochemical compensation after nigrostriatal bundle injury in an animal model of preclinical Parkinsonism. Arch Neurol 41:856–861.
    OpenUrlCrossRefPubMed
Back to top

In this issue

The Journal of Neuroscience: 21 (14)
Journal of Neuroscience
Vol. 21, Issue 14
15 Jul 2001
  • Table of Contents
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
The Role of Dopamine Receptors in Regulating the Size of Axonal Arbors
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
The Role of Dopamine Receptors in Regulating the Size of Axonal Arbors
C. L. Parish, D. I. Finkelstein, J. Drago, E. Borrelli, M. K. Horne
Journal of Neuroscience 15 July 2001, 21 (14) 5147-5157; DOI: 10.1523/JNEUROSCI.21-14-05147.2001

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
The Role of Dopamine Receptors in Regulating the Size of Axonal Arbors
C. L. Parish, D. I. Finkelstein, J. Drago, E. Borrelli, M. K. Horne
Journal of Neuroscience 15 July 2001, 21 (14) 5147-5157; DOI: 10.1523/JNEUROSCI.21-14-05147.2001
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • MATERIALS AND METHODS
    • RESULTS
    • DISCUSSION
    • Footnotes
    • REFERENCES
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • regeneration
  • dopamine receptors
  • sprouting
  • axonal arbor
  • dopamine antagonists
  • D1 receptor knock-out
  • D2 receptor knock-out
  • 6-OHDA lesions

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

ARTICLE

  • Functional Hemichannels in Astrocytes: A Novel Mechanism of Glutamate Release
  • A Precise Retinotopic Map of Primate Striate Cortex Generated from the Representation of Angioscotomas
  • Differential Role of Mitogen-Activated Protein Kinase in Three Distinct Phases of Memory for Sensitization in Aplysia
Show more ARTICLE

Development/Plasticity/Repair

  • The MAP3Ks DLK and LZK Direct Diverse Responses to Axon Damage in Zebrafish Peripheral Neurons
  • Rewiring cortico-muscular control in the healthy and post-stroke human brain with proprioceptive beta-band neurofeedback
  • Adenosine and Astrocytes Determine the Developmental Dynamics of Spike Timing-Dependent Plasticity in the Somatosensory Cortex
Show more Development/Plasticity/Repair
  • Home
  • Alerts
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Policy
  • Contact
(JNeurosci logo)
(SfN logo)

Copyright © 2022 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.