Fig. 1. Ascending visual and auditory pathways in normal and rewired wild-type mice. a, Schematic diagram of normal visual (blue) and auditory (orange) pathways. b, DiI labeling of the ascending auditory pathway at P0 in a control 129/SvEv mouse as seen in sagittal section under epifluorescence, showing axons streaming along the BIC from the IC to the MGB (arrow). The IC receives ascending axons that course through the LL. The cerebellar rudiment (Cb) is indicated for orientation.c, d, f, g,i, j, Coronal sections of the anterior MGB processed for CTB immunohistochemistry 1 d after intraocular injection of CTB. c, Unlesioned P1 control 129/SvEv mouse showing that no retinal axon terminals appear in the MGB of the neonate. DTN, Dorsal terminal nucleus;LTN, lateral terminal nucleus; vLGN, ventral nucleus of the lateral geniculate body.d, Unlesioned P1 ephrin-A2/A5 double knock-out mouse showing the absence of retinal axon terminals in the MGB of the neonate. e, Schematic diagram depicting unilateral rewiring surgery showing lesioned structures (SC,IC, and BIC) in gray.f, A coronal section through the midbrain of an adult control 129/SvEv mouse that received rewiring surgery as ine on P0, confirming unilateral ablation of SC and IC.g, A coronal section through the MGB in the same mouse as in f. Punctate staining within MGB indicates the presence of retinal axon terminals. h, Schematic diagram depicting rewiring surgery in which the IC and BIC were lesioned (shown in gray), but the SC was spared. i, Coronal section at the midcollicular level from an adult control 129/SvEv mouse that was lesioned as in h on P0, confirming the sparing of SC (lesioned side to theleft). j, The MGB in the same mouse as inh showing retinal innervation of the MGB induced by lesioning the IC. Scale bars: b, d, 100 μm; f, g, i,j, 150 μm. D, Dorsal; M, medial; P, posterior.