Open-System Approaches to Gene Expression in the CNS

A, The methodological steps in the TOGA cDNA display procedure, adapted from Sutcliffe et al. (2000).B, Automation in mRNA expression analysis: here a robot inserts bar-coded 96 well reaction tray into a computer-controlled PCR thermocycling machine. C, Comparison of TOGA mRNA expression profiles from eight regions of the mouse brain highlighting six hypothalamus-enriched mRNAs, three of which are already known (vasopressin, hypocretin, and oxytocin) and three of which are novel (Hy33, Hy88, and Hy94). D, A few thousand hypocretin-expressing neurons in the dorsolateral hypothalamus detected by in situ hybridization.