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ARTICLE, Cellular/Molecular

Demonstration by Fluorescence Resonance Energy Transfer of Two Sites of Interaction between the Low-Density Lipoprotein Receptor-Related Protein and the Amyloid Precursor Protein: Role of the Intracellular Adapter Protein Fe65

Ayae Kinoshita, Christa M. Whelan, Carolyn J. Smith, Irena Mikhailenko, G. William Rebeck, Dudley K. Strickland and Bradley T. Hyman
Journal of Neuroscience 1 November 2001, 21 (21) 8354-8361; https://doi.org/10.1523/JNEUROSCI.21-21-08354.2001
Ayae Kinoshita
1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
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Christa M. Whelan
1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
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Carolyn J. Smith
1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
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Irena Mikhailenko
2Department of Vascular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855
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G. William Rebeck
1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
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Dudley K. Strickland
2Department of Vascular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855
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Bradley T. Hyman
1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.21-21-08354.2001
PubMed 
11606623
Published By 
Society for Neuroscience
History 
  • Received May 14, 2001
  • Revision received July 27, 2001
  • Accepted August 17, 2001
  • First published November 1, 2001.
  • Version of record published November 1, 2001.
Copyright & Usage 
Copyright © 2001 Society for Neuroscience

Author Information

  1. Ayae Kinoshita1,
  2. Christa M. Whelan1,
  3. Carolyn J. Smith1,
  4. Irena Mikhailenko2,
  5. G. William Rebeck1,
  6. Dudley K. Strickland2, and
  7. Bradley T. Hyman1
  1. 1Alzheimer's Disease Research Unit, Department of Neurology, Massachusetts General Hospital, Charlestown, Massachusetts 02129, and
  2. 2Department of Vascular Biology, Holland Laboratory, American Red Cross, Rockville, Maryland 20855
View Full Text

Author contributions

Disclosures

    • Received May 14, 2001.
    • Revision received July 27, 2001.
    • Accepted August 17, 2001.
  • This work was supported by National Institutes of Health Grants AG12406 (B.T.H.), AG14473 (G.W.R.), and HL50784 (D.K.S.). A.K. was supported by the Uehara Memorial Foundation.

    Correspondence should be addressed to Dr. Bradley T. Hyman, Alzheimer Research Laboratory, Massachusetts General Hospital, 114 16th Street, Room 2009, Charlestown, MA 02129. E-mail:b_hyman{at}helix.mgh.harvard.edu.

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The Journal of Neuroscience: 21 (21)
Journal of Neuroscience
Vol. 21, Issue 21
1 Nov 2001
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Demonstration by Fluorescence Resonance Energy Transfer of Two Sites of Interaction between the Low-Density Lipoprotein Receptor-Related Protein and the Amyloid Precursor Protein: Role of the Intracellular Adapter Protein Fe65
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Demonstration by Fluorescence Resonance Energy Transfer of Two Sites of Interaction between the Low-Density Lipoprotein Receptor-Related Protein and the Amyloid Precursor Protein: Role of the Intracellular Adapter Protein Fe65
Ayae Kinoshita, Christa M. Whelan, Carolyn J. Smith, Irena Mikhailenko, G. William Rebeck, Dudley K. Strickland, Bradley T. Hyman
Journal of Neuroscience 1 November 2001, 21 (21) 8354-8361; DOI: 10.1523/JNEUROSCI.21-21-08354.2001

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Demonstration by Fluorescence Resonance Energy Transfer of Two Sites of Interaction between the Low-Density Lipoprotein Receptor-Related Protein and the Amyloid Precursor Protein: Role of the Intracellular Adapter Protein Fe65
Ayae Kinoshita, Christa M. Whelan, Carolyn J. Smith, Irena Mikhailenko, G. William Rebeck, Dudley K. Strickland, Bradley T. Hyman
Journal of Neuroscience 1 November 2001, 21 (21) 8354-8361; DOI: 10.1523/JNEUROSCI.21-21-08354.2001
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Keywords

  • amyloid precursor protein
  • APP
  • low-density lipoprotein receptor-related protein
  • LRP
  • adaptor protein Fe65
  • fluorescent resonance energy transfer
  • FRET
  • protein–protein interaction
  • secretory pathway
  • immunocytochemistry
  • confocal microscopy

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