Abstract
We examined the in vivo effects of the hallucinogen 4-iodo-2,5-dimethoxyamphetamine (DOI). DOI suppressed the firing rate of 7 of 12 dorsal raphe (DR) serotonergic (5-HT) neurons and partially inhibited the rest (ED50 = 20 μg/kg, i.v.), an effect reversed by M100907 (5-HT2A antagonist) and picrotoxinin (GABAA antagonist). DOI (1 mg/kg, s.c.) reduced the 5-HT release in medial prefrontal cortex (mPFC) to 33 ± 8% of baseline, an effect also antagonized by M100907. However, the local application of DOI in the mPFC increased 5-HT release (164 ± 6% at 100 μm), an effect antagonized by tetrodotoxin, M100907, and BAY × 3702 (5-HT1A agonist) but not by SB 242084 (5-HT2C antagonist). The 5-HT increase was also reversed by NBQX (AMPA–KA antagonist) and 1S,3S-ACPD (mGluR 2/3 agonist) but not by MK-801 (NMDA antagonist). AMPA mimicked the 5-HT elevation produced by DOI. Likewise, the electrical–chemical stimulation of thalamocortical afferents and the local inhibition of glutamate uptake increased the 5-HT release through AMPA receptors. DOI application in mPFC increased the firing rate of a subgroup of 5-HT neurons (5 of 10), indicating an enhanced output of pyramidal neurons. Dual-label fluorescence confocal microscopic studies demonstrated colocalization of 5-HT1Aand 5-HT2A receptors on individual cortical pyramidal neurons.
Thus, DOI reduces the activity of ascending 5-HT neurons through a DR-based action and enhances serotonergic and glutamatergic transmission in mPFC through 5-HT2A and AMPA receptors. Because pyramidal neurons coexpress 5-HT1A and 5-HT2A receptors, DOI disrupts the balance between excitatory and inhibitory inputs and leads to an increased activity that may mediate its hallucinogenic action.
- 5-hydroxytryptamine
- 5-HT1A receptors
- 5-HT2A receptors
- AMPA
- DOI
- dorsal raphe nucleus
- GABA
- glutamate
- hallucinogens
- medial prefrontal cortex
- microdialysis
- mGluR
- NMDA
- single-unit recordings
- thalamus
The prefrontal cortex plays a crucial role in higher brain functions (Fuster, 1997). It receives a dense innervation from the brainstem aminergic nuclei, including the serotonergic dorsal and median raphe nuclei of the midbrain (Azmitia and Segal, 1978). The prefrontal cortex contains a very large density of 5-HT1A and 5-HT2Areceptors located on pyramidal neurons. Likewise, GABAergic interneurons are enriched in 5-HT2A and 5-HT3 receptors (Pompeiano et al., 1992, 1994;Kia et al., 1996; Willins et al., 1997; Jakab and Goldman-Rakic, 1998,2000). Although the exact role of serotonergic neurotransmission in prefrontal cortex remains largely unknown (Robbins, 2000) serotonergic agents acting on cortical receptors are hallucinogenic [nonselective 5-HT2A agonists such as d-lysergic acid diethylamide-25 (LSD) or 4-iodo-2,5-dimethoxyamphetamine (DOI)] or have proven effective in the treatment of depression and schizophrenia (5-HT uptake inhibitors, 5-HT1Aagonists/5-HT2A antagonists) (Kroeze and Roth, 1998; Meltzer, 1999).
5-HT exerts complex actions on pyramidal neurons of the medial prefrontal cortex (mPFC). Previous work showed that 5-HT hyperpolarizes and depolarizes layer V pyramidal neurons by acting on 5-HT1A and 5-HT2 receptors, respectively (Araneda and Andrade, 1991), although no direct anatomical evidence for the colocalization of both receptors on individual pyramidal neurons has thus far been presented. More recently,Aghajanian and Marek (1997) demonstrated that the focal application of 5-HT on apical dendrites of pyramidal neurons in the mPFC evoked EPSCs by a 5-HT2A receptor-dependent mechanism. Likewise, the hallucinogen DOI, a partial 5-HT2A/2C receptor agonist, enhanced late EPSCs evoked by the electrical stimulation of afferent fibers (Aghajanian and Marek, 1999a). These effects are most likely mediated by a release of glutamate, triggered by the activation of 5-HT2A receptors, and the subsequent activation of AMPA receptors (Aghajanian and Marek, 1997, 1999a,b).
The apparent presence of 5-HT1A and 5-HT2A receptors in layer V pyramidal neurons suggests that they could be involved in the control of subcortical structures. The mPFC is one of the few forebrain areas projecting densely to the serotonergic dorsal raphe nucleus (DR) (Aghajanian and Wang, 1977; Sesack et al., 1989; Hajós et al., 1998; Peyron et al., 1998). Additionally, the electrical stimulation of the mPFC modulates the activity of 5-HT neurons in a complex manner (Hajós et al., 1998; Celada et al., 2001). Moreover, the activation of postsynaptic 5-HT1A receptors in the mPFC reduced the local release of 5-HT (Casanovas et al., 1999) and the firing rate of dorsal raphe 5-HT neurons (Celada et al., 2001). These data suggest that pyramidal neurons containing 5-HT1Areceptors may participate in the distal control of serotonergic activity. Because 5-HT1A and 5-HT2A receptors may colocalize in layer V pyramidal neurons projecting to the DR, 5-HT2Areceptors could be involved in this control. Therefore, in the present study we examined the actions of the hallucinogen DOI, a 5-HT2A/2C agonist, on the serotonergic system using in vivo microdialysis and single-unit recordings. The results obtained indicate that 5-HT2A receptor activation has profound effects on serotonergic neurons, likely via an enhancement of the AMPA-mediated neurotransmission in medial prefrontal cortex.
MATERIALS AND METHODS
Animals. Male albino Wistar rats (Iffa Credo, Lyon, France) weighing 280–320 gm and kept in a controlled environment (12 hr light/dark cycle and 22 ± 2°C room temperature) with food and water provided ad libitum, were used in in vivo experiments. Animal care followed the European Union regulations (O.J. of E.C. L358/1 18/12/1986).
Drugs and reagents. 5-HT oxalate, 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT), α-amino-3-hydroxy-5-methyli-4-soxazole-4-propionate [(S)-AMPA],bicuculline, cyclothiazide, 1-[2,5-dimethoxy-4-iodophenyl-2-amino-propane (DOI),l-trans-pyrrolidine-2,4-dicarboxylic acid (l-trans-PDC), (+)MK-801 (dizolcipine), NBQX, 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline, picrotoxininin, 6-chloro-5-methyl-1-[6-(2-methylpy-ridin-3-yloxy) pyridin-3-yl carbamoyl] indoline (SB 242084), tetrodotoxin (TTX), andN-(2-(4-(2-methoxyphenyl)-1-piperazinyl)ethyl)-N-(2-pyridyl) cyclohexanecarboxamide · 3HCl (WAY-100635), were from Sigma/Research Biochemicals (Natick, MA). 1S,3S-aminecyclopentane dicarboxylic acid (1S,3S-ACPD) was from Tocris (Bristol, UK). BAY × 3702, {R-(-)-2–237 4-[(chroman-2-ylmethyl)-amino]-butyl 253–1,1-dioxo-benzo[d]isothiazolone · HCl}, citalopram · HBr, LY 379268 {(−)-2-oxa-4-amino-bicyclo[3.1.0]hexane-4,6-dicarboxylate]}, and M100907 [R-(+)-α-(2,3-dime-thoxyphenil)-1-[4-fluorophenylethyl]-4-piperidinemethanol] were from Bayer AG, Lundbeck A/S, Eli Lilly & Co. (Indianapolis, IN), and Marion Merrel Dow (Strasbourg, France), respectively. A mouse monoclonal 5-HT2A antibody and a guinea pig polyclonal 5-HT1A antibody were obtained from PharMingen (San Diego, CA). Other materials and reagents were from local commercial sources. For the assessment of local effects, drugs were dissolved in the perfusion fluid and applied by reverse dialysis at the stated concentrations. Concentrated solutions (1 mm; pH adjusted to 6.5–7 with NaHCO3 when necessary) were stored frozen (−80°C), and working solutions were prepared daily by dilution. Concentrations are expressed as free bases. Control rats were perfused for the entire experiment with artificial CSF. The bars in the figures show the period of drug application (corrected for the void volume of the system). In experiments involving systemic administration, the drugs were administered subcutaneously or intravenously at the doses stated.
Surgery and microdialysis procedures. An updated description of the microdialysis procedures used can be found in Adell and Artigas (1998). Briefly, anesthetized rats (pentobarbital; 60 mg/kg, i.p.) were stereotaxically implanted with one concentric microdialysis probe equipped with a Cuprophan membrane in medial prefrontal cortex [anteroposterior (AP) +3.4, lateral (L) −0.8, dorsoventral (DV) −6.0; probe tip: 4 mm] (coordinates in millimeters; Paxinos and Watson, 1986). On the next day, microdialysis experiments were performed in freely moving rats. The probes were perfused at 1.5 μl/min with artificial CSF (aCSF) (in mm: 125NaCl, 2.5 KCl, 1.26 CaCl2, and 1.18 MgCl2) containing 1 μmcitalopram. After a 1 hr stabilization period, four fractions were collected to obtain basal values before local (reverse dialysis) or systemic administration of drugs. Successive 20 min (30 μl) dialysate samples were collected. In most experiments, the partial 5-HT2A/2C receptor agonist DOI was applied alone for 2 hr (six fractions). This was followed by the application of DOI in combination with other drugs for another 2 hr period. In experiments assessing the effect of l-trans-PDC on 5-HT release, the drug was perfused alone or in combination with other agents for 2 hr after collection of baseline values.
Three microdialysis experiments were conducted in chloral hydrate-anesthetized animals. In one of them, we examined the effect of the electrical stimulation of the midline thalamus projecting to mPFC, to modulate the release of 5-HT in the latter area. Microdialysis probes were implanted as above. Stimulating bipolar electrodes were used. The isolating material was peeled off ∼1 mm above the tip, the poles were separated 100–150 μm, and electrodes were stereotaxically placed and secured with dental cement, as for microdialysis probes. The tip of the electrodes was aimed at the top part of the centromedial thalamic nucleus (AP −3.6, L −0.7, DV −6.5). In this manner, the stimulated area was between DV −6.5 and DV −5.5, thus affecting the mediodorsal nucleus and part of the centromedial nucleus (plate 32;Paxinos and Watson, 1986). The lesion of this thalamic area has been shown to attenuate the EPSCs induced by 5-HT in layer V mPFC pyramidal neurons (Marek et al., 2001). On the next day, rats were anesthetized with chloral hydrate as in single-unit recording experiments (see below). After a 2 hr stabilization period, electrical stimulation was performed (5 Hz, 1.5 mA, 0.3 msec square pulses) for 20 min with a Grass S-48 stimulator unit S-48 connected to a Grass SIU isolation unit, and dialysate fractions were collected for one more hour. In some rats, the stimulating electrode was implanted, but no current was passed.
The second experiment examined the effects of the application of bicuculline in the midline thalamus (to disinhibit afferent fibers to mPFC) on the 5-HT release in mPFC. These rats were implanted with two dialysis probes, in mPFC (as above) and in the thalamus (coordinates: AP −3.2, L −0.5, DV −6.5; probe tip, 1.5 mm). In this location, the administration of bicuculline by reverse dialysis through the thalamic probe was also intended to affect both the centromedial and mediodorsal thalamic nuclei. The third experiment assessed the effects of the systemic administration of DOI (1 mg/kg, s.c.) on 5-HT release in the mPFC of chloral hydrate-anesthetized rats, to mimic the experimental conditions of single-unit recordings. At the end of the experiments, rats were killed by an overdose of sodium pentobarbital. The placement of the dialysis probes was examined by perfusion of Fast green dye and visual inspection of the probe track after cutting the brain at the appropriate levels. In experiments involving the electrical or chemical stimulation of thalamic afferents to mPFC, the placement of the thalamic probes or electrodes was verified histologically with Neutral Red staining.
The concentration of 5-HT in dialysate samples was determined by HPLC, as described (Adell and Artigas, 1998). 5-HT was separated using a Beckman (San Ramon, CA) 3 μm particle size column and detected with a Hewlett Packard 1049 electrochemical detector at +0.6 V. Retention time was between 3.5 and 4 min, and the limit of detection was typically 1 fmol/sample.
Single-unit recordings. We examined the effect of the systemic administration of DOI on the firing activity of identified 5-HT neurons in the dorsal raphe nucleus (DR). Single-unit extracellular recordings were performed as previously described (Sawyer et al., 1985; Celada et al., 1996). Briefly, rats were anesthetized (chloral hydrate, 400 mg/kg, i.p.) and positioned in a stereotaxic apparatus. Additional doses of chloral hydrate (80 mg/kg) were administered intravenously. Body temperature was maintained at 37°C throughout the experiment with a heating pad. All wound margins and points of contact between the animal and the stereotaxic apparatus were infiltrated with lidocaine solution (5%). To minimize pulsation, the atlanto-occipital membrane was punctured to release some CSF. For recordings in the DR, a burr hole of ∼4 × 4 mm was drilled over lambda, and the sagittal sinus was ligated, cut, and reflected. Single units in the DR were recorded extracellularly with glass micropipettes pulled from 2.0 mm capillary glass (World Precision Instruments, Sarasota, FL) on a Narishige (Tokyo, Japan) PE-2 pipette puller. Microelectrodes were filled with 2 m NaCl. Typically, impedance was between 4 and 10 MΩ. Descents were performed along the midline. Recorded 5-HT neurons were found 5.0–5.8 mm below the brain surface and were identified according to previously described electrophysiological criteria (Wang and Aghajanian, 1977). They exhibited a regular spontaneous firing rate with frequencies from 0.4 to 2.2 Hz, and 2–5 msec biphasic or triphasic extracellular waveform.
Single-unit potentials were amplified with a Neurodata IR283 (Cygnus Technology Inc., Delaware Water Gap, PA), postamplified, and filtered with a Cibertec amplifier (Madrid, Spain) and computed on-line using a DAT 1401plus interface system Spike2 software (Cambridge Electronic Design, Cambridge, UK). After recording stable baseline spontaneous activity for at least 5 min, DOI was administered (0.025–0.4 mg/kg, i.v.; cumulative doses) every 2 min. Once a maximal effect of DOI was attained, either M100907 (100 μg/kg, i.v.; Haddjeri et al., 1999) or the GABAA receptor antagonist picrotoxinin (1 mg/kg, i.v.) were injected. In some neurons, 8-OH-DPAT and WAY 100635, 5-HT1A receptor agonist and antagonist, respectively, were injected after M100907 or picrotoxinin to further confirm the serotonergic identity of the recorded cell. Only one neuron per rat was recorded.
Additional experiments were performed to examine the effects of the local application of DOI in mPFC on the firing rate of 5-HT neurons in the DR. DOI (200 μm, dissolved in aCSF) was infused through a 32 gauge stainless steel cannula (Small Parts Inc., Miami, FL) implanted in the mPFC (tip coordinates: AP +3.4, L −0.8, DV −4.5). The cannula was attached to a 10 μl Hamilton syringe by a Teflon tubing. A microinfusion pump (Bioanalytical Systems Inc., West Lafayette, IN) was used. After recording baseline spontaneous activity for at least 5 min, 200 nl of DOI was infused over the course of 1 min. This volume has been reported to diffuse to a maximum effective diameter of 0.4–0.6 mm (Myers, 1971). In some rats, a second DOI infusion was administered 5 min later.
Data and statistical analysis. Microdialysis results are expressed as femtomoles per fraction (uncorrected for recovery) and shown in figures as percentages of basal values (individual means of four predrug fractions). Statistical analysis of drug effects on dialysate 5-HT was performed using one- or two-way ANOVA for repeated measures of raw data with time as repeated factor and dose or pretreatment as independent factor. The effects of drugs or chemical stimulation on the 5-HT output were analyzed by one- or two-way ANOVA of raw data followed by post hoc Duncan test. Changes in firing rate were quantified by averaging the values in the second minute after drug injection and expressed as percentage of baseline. ED50 values were calculated with the GraphPad (San Diego, CA) Prism program. Data are expressed as the mean ± SEM. Statistical significance has been set at the 95% confidence level (two-tailed).
Cell culture and monoclonal antibody production. Stably transfected Chinese Hamster Ovary (CHO) cells expressing the 5-HT1A receptor were obtained from J. Raymond (Medical University of South Carolina, Charleston, SC). A monoclonal 5-HT1A receptor antibody was prepared as follows. Female BALB/c mice were immunized intraperitoneally with 50 μg of a synthetic peptide (NKRTPRRAAALISLC) conjugated toLimulus polyphemus hemocyanin and emulsified in Freund's adjuvant. Immune spleens were harvested after ∼3 months and fused with SP2/0 myeloma cells at a ratio of four spleen cells per myeloma using phenylethyleneglycol. Antibody-producing colonies were detected by ELISA as follows: immunizing peptide conjugated to BSA (10 μg/ml) was immobilized to COSTAR 9017 EIA/RIA plates in 100 μl of coating buffer (50 mmcarbonate–bicarbonate, pH 9.6) and incubated overnight at room temperature. Wells were blocked with 2% milk in PBS (blocking buffer) 1 hr, followed by addition of antibody in culture supernatant or in blocking buffer for 90 min. Antibodies were detected using a horse anti-mouse polyclonal antibody linked to horseradish peroxidase (Vector Laboratories, Burlingame, CA; 1:2000 in blocking buffer) followed by spectrophotometric detection at 405 nm using the 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) substrate system (Sigma, St. Louis, MO). Hybridomas were subjected to three rounds of limiting dilution cloning, after which antibody was produced in bulk by generation of ascites in histocompatible mice. Immunoreactivity of the antibodies was confirmed both by ELISA (as described above) and by immunohistochemical staining using CHO cells (control) or CHO cells stably expressing the 5-HT1A receptor. Briefly, cells on microscope coverslips in 24 well microtiter plates were serum-starved overnight at 37°C and 5% CO2, then fixed in 4% paraformaldehyde and maintained at room temperature. Cells were permeabilized 20 min with 0.1% Triton X-100 and blocked with 2% milk for 1 hr. Anti-5-HT1A antibody supernatants were incubated with the cells for 90 min, followed by incubation for 1 hr with a Texas Red-labeled goat anti-mouse secondary reagent (Vector Laboratories). Cells were then mounted for confocal microscopy as previously described (Willins et al., 1997, 1999).
Confocal immunofluorescence studies. Rats were prepared for immunofluorescence studies as previously detailed (Willins et al., 1997, 1999), and free-floating 40 μm sections of mPFC were prepared as described previously (Willins et al., 1997, 1999). Free-floating sections were then incubated in blocking buffer (PBS containing 0.2% Triton X-100, 5% nonfat dry milk, and 1% goat serum) for 1 hr at room temperature. Sections were then incubated for 1 hr at room temperature with a 1:3000 dilution of polyclonal 5-HT2A-selective antibody (Berry et al., 1996) and a 1:10 dilution of a monoclonal 5-HT1Aantibody in blocking buffer. Additional sections were incubated with a commercial monoclonal 5-HT2A receptor antibody (1:500; PharMingen) and a commercial guinea pig polyclonal 5-HT1A receptor antibody (1:2000; PharMingen). Sections were then incubated overnight at 4°C with constant agitation. After extensive washing with PBS, sections were incubated with a 1:200 dilution of secondary antibodies (BODIPY-FL-anti-mouse and Texas Red anti-rabbit or Texas Red anti-guinea pig) for 1 hr at room temperature followed by extensive washing with PBS and mounting for confocal microscopy as previously detailed (Willins et al., 1997,1999). Controls included incubation of sections with secondary antibody alone.
Dual-label confocal microscopy was performed using a Zeiss Model 410 confocal microscope as previously described (Willins et al., 1997,1999), and images were processed using Adobe Photoshop.
RESULTS
Baseline 5-HT values
The basal concentration of 5-HT in dialysates from medial prefrontal cortex was 17.5 ± 0.8 fmol/fraction (n= 105) in freely moving rats and 17.0 ± 1.1 fmol/fraction (n = 24) in chloral hydrate-anesthetized rats.
Effects of the local application of DOI in medial prefrontal cortex
The local application of 1, 10, and 100 μm DOI (2 hr each) increased the 5-HT output in a concentration-dependent manner. Maximal increases at each concentration were, respectively 119 ± 20, 137 ± 16, and 209 ± 29% of baseline (n= 5) (Fig. 1A). One-way ANOVA showed a significant effect of DOI application (p < 0.00001). In subsequent experiments, we used the concentration of DOI that elicited maximal effects (100 μm).
A, Concentration-dependent increase of 5-HT in dialysates from medial prefrontal cortex evoked by the local application of 1, 10, and 100 μm of the partial 5-HT2A/2C receptor agonist DOI (filled circles; n = 5). The bar graph in theinset shows the average increase in 5-HT obtained from the last four (stabilized) fractions at each DOI concentration (+p < 0.05 vs baseline).B, Effect of the continuous application of 100 μm DOI (filled squares;n = 7). Control rats (n = 6) received artificial CSF throughout the experiment. Bars show the period of drug application. +p < 0.05 versus baseline; Duncan test post-ANOVA.
The perfusion of 100 μm doi (n = 7) significantly increased the 5-HT output when compared with the control group (artificial CSF; n = 7) (p< 0.02, treatment effect; p < 0.00001, time effect;p < 0.00001 time × treatment interaction) (Fig.1B). Maximally elevated 5-HT values were observed soon (40 min) after DOI application and remained elevated over the entire period of application. The mean increase produced by 100 μm DOI in all perfusion experiments was 164 ± 6% of baseline (average of fractions 7–10;n = 69).
The effect of DOI was completely antagonized by the coperfusion of 1 μm TTX, which reduced 5-HT values below baseline (from 185 ± 10 to 30 ± 6% of baseline; n = 4;p < 0.00001) (Fig. 2). Likewise, the 5-HT increase produced by 100 μmDOI was significantly attenuated by the coperfusion of 100 μm of the selective 5-HT2A receptor antagonist M100907 (DOI: 181 ± 8% of baseline; DOI + M100907: 119 ± 13% of baseline;n = 8; p < 0.00001) (Fig.3A). A higher M100907 concentration (300 μm) elicited a greater reduction of the 5-HT output (from 163 ± 1% to 42 ± 6% of baseline, n = 6; p < 0.00001) (Fig.3A). In contrast, the selective 5-HT2Creceptor antagonist SB 242084 did not reverse the 5-HT elevation produced by DOI and increased the 5-HT output further, from 178 ± 22% to 224 ± 25% of baseline (n = 6;p < 0.00001) (Fig. 3B).
The perfusion of tetrodotoxin (TTX; 1 μm) reversed the increase in 5-HT output induced by the local application of DOI 100 μm (n = 4). Bars show the period of drug application. The effect of DOI alone is shown by a dotted line.+p < 0.05 versus baseline (DOI alone); *p < 0.05 versus DOI alone (DOI + drug); Duncan test post-ANOVA.
A, The application of the selective 5-HT2A receptor antagonist M100907 (100 and 300 μm; filled triangles andcircles, n = 8 and 4, respectively) antagonized the increase in 5-HT output induced by the local application of DOI 100 μm. B, Lack of antagonism of the effect of DOI by the selective 5-HT2C receptor antagonist SB 242084 (100 μm;filled circles, n = 6). This drug significantly potentiated the 5-HT increase elicited by DOI (shown by adotted line). +p < 0.05 versus baseline (DOI alone); *p < 0.05 versus DOI alone (DOI + drug); Duncan test post-ANOVA.
The perfusion of the noncompetitive NMDA receptor antagonist MK-801 (300 μm) did not significantly modify the effect of DOI (n = 7) (Fig.4A). In contrast, the application of the AMPA–KA receptor antagonist NBQX (300 μm) significantly reduced the effect of DOI (from 182 ± 15% to 122 ± 13% of baseline;n = 6; p < 0.00001) (Fig.4B). The perfusion of the preferential mGluR 2/3 agonist 1S,3S-ACPD (300 μm) modestly reversed the effect of DOI on 5-HT output only in the last two fractions (n = 5;p < 0.00001) (Fig. 4C). A higher concentration of 1S,3S-ACPD (1 mm) elicited a more marked antagonism and almost completely reversed the effect of DOI, from 190 ± 15 to 112 ± 6% of baseline (n = 5; p < 0.00001) (Fig. 4C). In contrast, the application of the selective mGluR 2/3 agonist LY 379268, perfused at three different concentrations (300 μm, 1 mm, and 3 mm) failed to significantly reduce the 5-HT increase induced by the local application of DOI. The average effect of LY 379268 was 96 ± 16, 97 ± 12, and 107 ± 10% at 300 μm, 1 mm, and 3 mm, respectively (n = 4–5, data expressed as percentage of the 5-HT values during DOI alone). The effect of DOI was mimicked by the local application of AMPA (300 μm), which induced a maximal elevation of 5-HT to 188 ± 15% of baseline (p < 0.001, treatment effect; p< 0.00001, time effect; p < 0.00001, time × treatment interaction compared with controls; n = 6) (Fig. 4D).
A, The application of the noncompetitive NMDA receptor antagonist MK-801 (300 μm) did not reverse the increase in 5-HT output induced by DOI 100 μm (n = 7). B,Antagonism by the AMPA–KA antagonist NBQX (300 μm) of the effect of DOI (n = 7). C, The mGluR 2/3 agonist 1S,3S-ACPD (300 μm, filled triangles; 1 mm,filled circles; n = 5 each) reversed the 5-HT elevation induced by DOI. D, The perfusion of 300 μm AMPA elevated the 5-HT similarly to DOI (n = 6; filled circles). The effect of DOI alone is shown by a dotted line.+p < 0.05 versus baseline (DOI or AMPA); *p < 0.05 versus DOI alone (DOI + drug); Duncan test post-ANOVA.
The increase in 5-HT output produced by the application of DOI was strongly antagonized by the coperfusion of the selective 5-HT1A receptor agonist BAY × 3702 (De Vry et al., 1998; Casanovas et al., 2000) (30 μm; from 189 ± 30 to 60 ± 13% of baseline; n = 5;p < 0.00001) (Fig.5).
The selective 5-HT1A receptor agonist BAY × 3702 (30 μm) reversed the DOI-induced increase in 5-HT output (n = 5). The effect of DOI alone is shown by a dotted line.+p < 0.05 versus baseline (DOI alone); *p < 0.05 versus DOI alone (DOI + drug); Duncan test post-ANOVA.
Localization of 5-HT1A and 5-HT2A receptors on individual mPFC pyramidal neurons
The opposite effects of 5-HT1A and 5-HT2A receptor activation in mPFC on 5-HT release are in keeping with previous data on pyramidal neuron excitability (Araneda and Andrade, 1991). However, despite abundant literature on the localization of either type in cortical neurons (see introductory remarks), it is unclear whether both receptors are coexpressed in pyramidal neurons. To address this question, we prepared a monoclonal 5-HT1A receptor antibody suitable for immunochemical studies. In preliminary studies (D. A. Shapiro and B. L. Roth, unpublished observations) we observed that the monoclonal 5-HT1A receptor antibody specifically labeled 5-HT1A receptors stably expressed in CHO cells but did not visualize untransfected CHO cells. As shown in Figure6A–F, 5-HT1A and 5-HT2A receptors were abundantly colocalized on individual mPFC pyramidal neurons. Control experiments using secondary antibodies alone showed no specific immunofluorescence (Fig. 6I). Additional studies using commercially available antibodies directed against distinct epitopes of the 5-HT1A and 5-HT2A receptors (Fig. 6G,H) also showed extensive colocalization of 5-HT1Aand 5-HT2A receptors in individual cortical pyramidal neurons.
Colocalization of 5-HT2A and 5-HT1A receptors in individual cortical neurons.A shows a representative mPFC section depicting individual cortical neurons expressing 5-HT1A-like immunoreactivity; B shows the same section with 5-HT2A-like immunoreactivity; C depicts an overlay showing colocalization of 5-HT2A and 5-HT1A receptor-like immunoreactivity in individual cortical neurons. Magnification, 400×. D shows a high-power (1000×) view of individual cortical neurons expressing 5-HT1A-like immunoreactivity; E shows the same section with 5-HT2A-like immunoreactivity;F depicts an overlay of 5-HT1A and 5-HT2Alike immunoreactivity. G shows a low-power (400×) view of cortical neurons visualized with a guinea pig 5-HT1A receptor antibody, whereas H shows the same section visualized with a monoclonal 5-HT2Areceptor antibody, with colocalization indicated byarrowheads. I shows a typical section incubated only with secondary antibodies (400×).
Effects of increased glutamatergic transmission in mPFC on serotonergic function
The above microdialysis results suggested that DOI increased 5-HT release in mPFC indirectly, possibly by (1) enhancing a glutamatergic tone on 5-HT nerve terminals (local effect) and/or (2) by enhancing the activity of ascending 5-HT neurons in the DR through descending excitatory inputs. We therefore examined the effects of directly increasing the glutamatergic activity in mPFC on the local 5-HT release.
We first studied whether the 5-HT-increasing effects of DOI could be mimicked by endogenous glutamate. To this end, we electrically stimulated the mediodorsal and centromedial thalamic nuclei (5 Hz, 1.5 mA, 0.3 msec) for 20 min and collected dialysate fractions from the ipsilateral mPFC. The electrical stimulation of these nuclei elevated the 5-HT output in mPFC to a maximum of 179 ± 31% of baseline (p < 0.00001; n = 6) (Fig.7A). Sham stimulation did not alter 5-HT levels (n = 6) (Fig. 7A). Likewise, in dual probe microdialysis experiments, the application of bicuculline (300 μm and 1 mm) by reverse dialysis in the mediodorsal and centromedial thalamic nuclei doubled the 5-HT release in mPFC (n = 8; p < 0.0007) (Fig.7B).
A, The electrical stimulation of a midline thalamic area affecting the mediodorsal nucleus and a small part of the centromedial nucleus (5 Hz, 1.5 mA, 0.3 msec pulses; shown by a bar) for 20 min increased the 5-HT release in the mPFC of chloral hydrate-anesthetized rats (filled triangles;n = 6). Control rats were implanted with microdialysis probes and thalamic electrodes, but no current was passed (n = 6; open circles). *p < 0.05 versus baseline; Duncan test post-ANOVA.B, In dual probe microdialysis experiments, the application of bicuculline (BIC; 300 μm and 1 mm, shown by a bar; n = 8) by reverse dialysis in the centromedial and mediodorsal thalamic nuclei significantly increased the 5-HT release in mPFC. The variability of the 5-HT increase was relatively large, possibly because of slight differences in the location of probes. *p < 0.05 versus baseline; Duncan test post-ANOVA. C, Effects of the local application of the glutamate uptake inhibitorl-trans-PDC, alone or in combination with NBQX, MK-801, and cyclothiazide on the 5-HT release in mPFC (n = 4–5).l-trans-PDC (4 mm) significantly increased the 5-HT release. This effect was prevented by the coperfusion of NBQX but not by MK-801 (300 μm each). Cyclothiazide (300 μm), a drug that prevents the desensitization of AMPA receptors, did not affect the time course or potentiate the effect of l-trans-PDC, suggesting that the marked tachiphylaxis of the effect is possibly caused by a presynaptic adaptive change in glutamate synthesis and release resulting from prolonged inhibition of reuptake.+p < 0.05 versus baseline; *p < 0.05 versusl-trans-PDC alone; Duncan test post-ANOVA.
Moreover, the local (in mPFC) application of the glutamate uptake inhibitor l-trans-PDC markedly increased the local 5-HT release (n = 4; p < 0.000001) (Fig. 7C). The time course of the effect differed from that elicited by AMPA (Fig. 4D) and showed a marked tachyphylaxis. However, the 5-HT increase was fully prevented by the coapplication of 300 μm NBQX (p < 0.00005 vsl-trans-PDC alone) (Fig.7C) but not by 300 μm MK-801, thus supporting the involvement of AMPA–KA receptors. The coapplication of 300 μm cyclothiazide to prevent a putative desensitization of the glutamate receptors involved in this effect failed to modify the effect ofl-trans-PDC, suggesting that the transient nature of the 5-HT increase was likely attributable to presynaptic adaptive mechanisms after glutamate uptake blockade.
Effects of DOI on the firing rate of 5-HT neurons in the DR
The systemic administration of DOI to chloral hydrate-anesthetized rats induced a dose-dependent reduction of the firing rate of 5-HT neurons in the DR (Fig.8). Two putative subgroups of neurons were identified, according to the maximal effect of DOI. In 7 of 12 neurons recorded, DOI fully suppressed the serotonergic activity at low doses. In the rest (n = 5), the effect of DOI on serotonergic cell firing was partial, with a maximal decrease to ∼40% of baseline. The administration of additional doses of DOI did not result in a further reduction. In some neurons, higher doses of DOI appeared to elicit a slight increase in firing rate, as shown in Figure8B. In both subgroups, the effect of DOI was reversed by the administration of M100907 (100 μg/kg, i.v.) (Fig.8B,D). The calculated ED50 value was 20 μg/kg intravenously for all neurons (19 and 22 μg/kg, i.v., for neurons with full and partial inhibitory response, respectively). When examined, the administration of the 5-HT1Areceptor agonist 8-OH-DPAT suppressed serotonergic cell firing in both subgroups of neurons (Fig. 8C,D). The further administration of WAY 100635 (10 μg/kg, i.v.) returned firing rate to baseline (Fig. 8D). The inhibitory effect of DOI on serotonergic cell firing was also reversed by the administration of the GABAA receptor antagonist picrotoxinin (1 mg/kg, i.v.) (Fig. 8E).
Effects of the intravenous administration of DOI on serotonergic neurons of the DR. A–E, Integrated firing rate histograms showing the effect of DOI on five different 5-HT neurons. A–C depict neurons with full (A) and partial (B, C) inhibitory responses to the administration of cumulative doses of DOI (25–50 μg/kg, i.v. in A; 25–400 μg/kg, i.v. inB, C). The effect of DOI was antagonized by M100907 (100 μg/kg, i.v.; examples in B andD). The serotonergic nature of neurons with partial or full response to DOI is illustrated by its sensitivity to 8-OH-DPAT (C, D). D shows the inhibitory effect of DOI (25–50 μg/kg, i.v.). After reversal by M100907 (100 μg/kg, i.v.), 8-OH-DPAT (DPAT; 0.25–2 μg/kg, i.v.) fully suppressed firing activity, and the 5-HT1A receptor antagonist WAY 100635 (WAY; 10 μg/kg, i.v.) returned firing rate to baseline. E shows the reversal of the effect of DOI (25–200 μg/kg, i.v.) by the GABAAantagonist picrotoxinin (PTX; 1 mg/kg, i.v.). The graph inF shows the dose–response curves for DOI in 5-HT neurons. The neurons with partial and full inhibitory responses to DOI administration had comparable ED50 values (19 and 22 μg/kg, i.v.; n = 7 and 5, respectively). Thedotted line shows the dose–response curve for all neurons (n = 12).
To assess whether the increased mPFC release of 5-HT induced by local DOI application was caused by the activation of pyramidal neurons projecting to the DR (thus enhancing the activity of ascending 5-HT neurons), we locally infused DOI in the mPFC while recording 5-HT neurons in the DR. In 5 of 10 serotonergic neurons in the DR, the application of DOI in mPFC (200 μm, 200 nl applied in 1 min) enhanced their firing rate (to 194 ± 45% of baseline; range, 137–371%; p < 0.02) (Fig.9A). One additional neuron had a marginal increase (115% of baseline), and the rest were unaffected by the application of DOI in mPFC (Fig. 9B). The effects of a second dose of DOI (5 min after the first one) were examined in seven neurons. Of these, three responded with a further increase in DR firing (from 155 to 260% of baseline). The infusion of vehicle in mPFC did not affect the firing rate of DR 5-HT neurons (data not shown).
Integrated firing rate histograms of DR serotonergic neurons after the application of DOI in mPFC (200 μm, 200 nl, shown by a 1 min bar). The neuron inA responded with an increase in firing rate after the first and second application of DOI (note the persistence of the increased firing rate), whereas the neuron in B was unaffected by the application of two consecutive doses of DOI. The distal effect of DOI on 5-HT cell firing cannot be accounted for by drug diffusion to the DR because of the small amount of tissue affected by DOI application (0.2 μl) and the inhibitory effect of DOI on 5-HT neurons when acting on DR 5-HT2A receptors (Liu et al., 2000).
Effect of the systemic administration of DOI on 5-HT release in medial prefrontal cortex
The systemic administration of DOI (1 mg/kg, s.c.) to chloral hydrate-anesthetized rats reduced the extracellular 5-HT concentration in the mPFC to a maximal effect of 33 ± 8% of baseline 2 hr after administration (n = 4; p < 0.00001) (Fig. 10). The effect of DOI was reversed by the administration of M100907 (0.5 mg/kg, s.c.) which returned 5-HT levels to above baseline (127 ± 6%; p < 0.00001) (Fig. 10).
The systemic administration of DOI (1 mg/kg, s.c.) markedly reduced the 5-HT output in mPFC of chloral hydrate-anesthetized rats (n = 4). The selective 5-HT2A receptor antagonist M100907 (0.5 mg/kg, s.c.) fully reversed the effect of DOI. Arrows mark injections.+p < 0.05 versus baseline; *p < 0.05 versus DOI alone; Duncan test post-ANOVA.
DISCUSSION
The present results show that the stimulation of 5-HT2A receptors by the hallucinogen DOI markedly affects serotonergic transmission. Systemic DOI administration reduced the activity of DR 5-HT neurons and their release by mPFC terminals. Conversely, the selective activation of prefrontal 5-HT2A receptors raised the local 5-HT release and the firing of a subgroup of 5-HT neurons.
Despite the low density of 5-HT2A receptors in the DR and adjacent areas (Pompeiano et al., 1994; Fay and Kubin, 2000), bath application of 5-HT and DOI increased a 5-HT2A receptor-dependent GABA input onto 5-HT neurons in midbrain slices (Liu et al., 2000). Thus, the activation by DOI of 5-HT2A receptors on GABAergic afferents to 5-HT neurons might mediate its inhibitory effect on 5-HT neurons (Wright et al., 1990; Garratt et al., 1991; this work) inasmuch as the activation of prefrontal 5-HT2A receptors induced the opposite effect. The involvement of 5-HT2Aand GABAA receptors in the DOI-induced inhibition of serotonergic cell firing is supported by the reversal induced by M100907 and picrotoxinin. Interestingly, DOI partially inhibited the firing activity of some 5-HT neurons, whereas fully suppressed other 5-HT neurons, with comparable ED50 values. This difference suggests either a partial agonist action of DOI or the involvement of two 5-HT2A receptor populations (e.g., in midbrain and mPFC) with opposed effects on the cell firing of some neurons.
Unlike its systemic administration (Wright et al., 1990; Gobert and Millan, 1999; this work) the local application of DOI enhanced the 5-HT release in mPFC. We exclude a direct releasing action of DOI on serotonergic nerve endings (fenfluramine-like) because this is insensitive to TTX (Carboni and Di Chiara, 1989). Moreover, 5-HT neurons do not express 5-HT2A receptors (Pompeiano et al., 1994; Fay and Kubin, 2000) (J. Serrats, R. Cortés, and G. Mengod, unpublished observations). Some cells in the DR (not known to be serotonergic) express 5-HT2C receptors (Clemett et al., 2000). However, the lack of antagonism by SB 242084 excludes the involvement of 5-HT2C receptors in the effect of DOI.
Several lines of evidence suggest that DOI increases the 5-HT release in mPFC indirectly, through the stimulation of glutamate release and subsequent activation of AMPA–KA receptors (Fig.11). First, the 5-HT increase was reversed by NBQX but not by MK-801 and was mimicked by AMPA application. Second, several strategies aimed at increasing glutamatergic neurotransmission in the mPFC, such as the perfusion of the glutamate reuptake inhibitor l-trans-PDC (also enhancing striatal 5-HT release; Abellán et al., 2000) or the electrical–chemical stimulation of thalamic afferents to the mPFC elevated the 5-HT release. Third, as with DOI, the effect ofl-trans-PDC was antagonized by NBQX but not by MK-801. Finally, the effect of DOI was reversed by the mGluR 2/3 agonist 1S,3S-ACPD. The failure of the more selective agonist LY 379268 to counteract the DOI-induced 5-HT increase may be attributed to different in vivo pharmacological properties and/or involvement of other mGluR subtypes in the action of ACPD. Thus, LY 379268 increased 5-HT turnover and release in mPFC by itself (Cartmell et al., 2000, 2001), an effect that would oppose the expected reduction in 5-HT resulting from a decrease in glutamate. This difference warrants further investigation on the mGluR subtype involved in the control of 5-HT release in mPFC.
Scheme of the putative presynaptic and postsynaptic actions of DOI. In the mPFC, DOI increases 5-HT release. This effect involves the activation of prefrontal 5-HT2Areceptors, possibly on glutamatergic afferents from midline thalamus. The role of 5-HT2A receptors on pyramidal neurons and GABA interneurons has not been elucidated. The activation of prefrontal 5-HT2A receptors enhances the release of glutamate, which acts on pyramidal AMPA receptors and increases impulse flow in layer V pyramidal neurons projecting to the DR, thus resulting in an increased serotonergic activity and 5-HT release in mPFC. Additionally, glutamate might activate terminal AMPA–KA receptors in 5-HT terminals and increase 5-HT release in a local manner. This might imply axo-axonic contacts between glutamatergic and serotonergic axons, not yet established anatomically but for which there is functional evidence (see Discussion). The activation of inhibitory 5-HT1Areceptors on pyramidal neurons counteracts the effects of DOI and decreases 5-HT release, likely by reducing DOI excitatory effects on descending inputs to the DR, because the selective activation of prefrontal 5-HT1A receptors reduces the firing rate of DR 5-HT neurons (Celada et al., 2001). The firing-suppressant action of systemic DOI administration on DR 5-HT neurons is possibly accounted for by an action on local GABAergic elements inhibiting 5-HT neurons (Liu et al., 2000). This effect would also account for the inhibition of 5-HT release observed after systemic administration of DOI.
A critical step in the proposed mechanism is the actual assessment of the effect of DOI on glutamate release. However, extracellular glutamate concentrations are not representative of the transmitter–synaptic pool (Timmerman and Westerink, 1997) and depend to a large extent on a cysteine–glutamate exchanger (Baker and Kalivas, 2000). Our own data agree with this view, because extracellular glutamate was largely insensitive to manipulations known to alter synaptic transmission (M.V. Puig, A. Adell, P. Celada, and F. Artigas, unpublished observations).
The present results accord with previous in vitro work in mPFC slices showing that the activation of local 5-HT2A receptors increases spontaneous EPSCs in layer V pyramidal neurons by an AMPA-dependent mechanism (Aghajanian and Marek, 1997, 1999b, 2000; Marek et al., 2000). However, none of these previous studies reported overt depolarization in pyramidal neurons by 5-HT or DOI. In contrast, we observed that the stimulation of 5-HT2A receptors in mPFC by DOI increased the firing of identified 5-HT neurons in the DR. Likewise, in pilot experiments, systemic DOI administration activated projection neurons in the mPFC (Puig, Celada, and Artigas, unpublished observations). This strongly suggests that DOI increases impulse flow in pyramidal neurons, including those projecting to the DR. The discrepancy between the present results and those of Aghajanian and Marek (2000) may be attributable to methodological differences, perhaps reflecting the loss of active glutamatergic inputs in the slice preparation used by these authors.
The cellular source or sources of glutamate and the precise localization or localizations of 5-HT2A receptors accounting for this effect are debated. 5-HT2Areceptors occur in three different populations in frontal cortex: pyramidal neurons (major 5-HT2A-containing neurons), GABAergic interneurons, and axon terminals (Willins et al., 1997; Jakab and Goldman-Rakic, 1998, 2000; Xu and Pandey, 2000). Layer V contains the somata of pyramidal neurons and dense bundles of apical dendrites, highly enriched in 5-HT2A receptors (Willins et al., 1997; Jakab and Goldman-Rakic, 1998; Xu and Pandey, 2000) (Fig. 6). GABA interneurons are the main target of 5-HT terminals in monkey prefrontal cortex (Smiley and Goldman-Rakic, 1996) and DOI increases extracellular GABA in rat brain (Abi-Saab et al., 1999). However, 5-HT2A receptors on thalamic afferents have been suggested to mediate DOI effects. Hence, μ-opioid agonists and lesions of the medial thalamus attenuated the 5-HT-induced EPSCs in layer V pyramidal neurons (Marek and Aghajanian, 1998; Marek et al., 2001), which suggests a presynaptic site of action. Moreover, the systemic administration of DOI increased Fos expression in superficial layer V and above, an effect also dependent on the integrity of thalamic inputs onto mPFC (Scruggs et al., 2000). However, most Fos-expressing neurons were nonpyramidal, whereas 5-HT induces spontaneous EPSCs in layer V pyramidal neurons by activation of 5-HT2A receptors (Lambe et al., 2000), which makes uncertain the relationship between both observations. The present data suggest that glutamate from thalamic afferents to mPFC enhances 5-HT release, although it remains to be determined whether this is the glutamate source involved in the local DOI effects.
Indeed, a preferential action of DOI on terminal 5-HT2A receptors would leave the dense population of pyramidal 5-HT2A receptors without a significant role. Interestingly, the DOI-induced increase in 5-HT release was potently counteracted by coperfusion of the selective 5-HT1A receptor agonist BAY × 3702. 5-HT1A and 5-HT2A receptors colocalize on cortical pyramidal neurons (Fig.6A–I) and have opposite effects on their excitability (Araneda and Andrade, 1991). The apparent absence of 5-HT1A receptors on 5-HT terminals (Pompeiano et al., 1992; Kia et al., 1996) excludes a presynaptic action of BAY × 3702. Pyramidal neurons in mPFC project to and control the activity of DR 5-HT neurons (see introductory remarks). Thus, DOI and BAY × 3702 may modulate the release of 5-HT in mPFC by increasing and decreasing, respectively, the activity of descending excitatory pathways to DR neurons projecting in turn to the mPFC (Fig. 11). Thus, the application of DOI and 8-OH-DPAT in mPFC increased and decreased, respectively, the firing rate of DR 5-HT cells (Celada et al., 2001; this work). However, the present study cannot determine whether the effect of DOI on pyramidal activity is direct (via pyramidal 5-HT2A receptors) or indirect (via terminal 5-HT2A receptors, increase in glutamate and activation of AMPA receptors on pyramidal neurons) (Fig. 11). Additionally, the activation of terminal AMPA receptors increases transmitter release in vivo and in vitro (Ohta et al., 1994; Whitton et al., 1994; Maione et al., 1997, Tao et al., 1997;Lockhart et al., 2000). This raises the possibility that a second (local) mechanism may also modulate 5-HT release independently of DR activation.
5-HT2A receptors play a crucial role in the filtering of inputs reaching the somata of pyramidal neurons because their activation increases neuronal excitability. The activation by DOI of prefrontal 5-HT2A receptors increases the excitability of pyramidal neurons. At the same time, its action on (possibly) midbrain 5-HT2A receptors (Liu et al., 2000) reduces 5-HT cell firing and 5-HT release, which should result in a diminished activation of inhibitory pyramidal 5-HT1A receptors. Hence, the net balance of both effects would be an increased firing activity of projection neurons in layer V, as preliminarily observed (Puig, Celada, and Artigas, unpublished observations). This effect may mediate the hallucinogenic action of DOI. Hence, the blockade of 5-HT2A receptors or the direct or functional 5-HT1A receptor agonism exerted by atypical antipsychotics (Meltzer, 1999; Ichikawa et al., 2001) might be viewed as an attempt to restore the physiological balance between excitatory and inhibitory inputs onto prefrontal pyramidal neurons. Given the opposing roles of 5-HT1A and 5-HT2A receptors on the output of layer V pyramidal neurons, compounds with 5-HT2Aantagonism and 5-HT1A agonism may be particularly useful to treat hallucinations and, more generally, positive symptoms in schizophrenia. Likewise, their direct action at 5-HT1A receptors might also improve negative (deficit) symptoms.
Further work is required to clarify the relative role of local and distal mechanisms in the control of 5-HT release by prefrontal 5-HT1A/2A receptors and the precise localization of 5-HT2A receptors responsible for this effect of DOI.
Footnotes
This work was supported by National Institutes of Health Grants FIS 01/1147 and SAF01–2133 (F.A.), KO2MH01366 and RO1MH61887 (B.L.R.), and a National Alliance for Research on Schizophrenia and Depression Young Investigator Award to D.A.S. M.V.P. is recipient of a predoctoral fellowship from the Institut d'Investigacions Biomèdiques August Pi i Sunyer. Financial support from Bayer S.A. is also acknowledged. We thank the pharmaceutical companies for the generous supply of drugs. The technical help of Leticia Campa is gratefully acknowledged.
R.M.R. and M.V.P. contributed equally to this work.
Correspondence should be addressed to Dr. Francesc Artigas, Department of Neurochemistry, Institut d' Investigacions Biomèdiques de Barcelona (Consejo Superior de Investigaciones Cientı́ficas), Institut d'Investigacions Biomèdiques August Pi i Sunyer, Rosselló, 161, Sixth floor, 08036 Barcelona, Spain. E-mail:fapnqi{at}iibb.csic.es.