ARTICLE, Cellular/Molecular

Co-Expression of Putative Pheromone Receptors in the Sensory Neurons of the Vomeronasal Organ

Sara Martini, Lucia Silvotti, Arild Shirazi, Nicholas J. P. Ryba and Roberto Tirindelli
Journal of Neuroscience 1 February 2001, 21 (3) 843-848; https://doi.org/10.1523/JNEUROSCI.21-03-00843.2001

Abstract

Two large and divergent families of G-protein-coupled receptors (V1Rs and V2Rs) are expressed in subsets of neurons in the vomeronasal organ. These receptors are likely to mediate pheromone responses, but it appears that many V2R genes may encode expressed pseudogenes rather than functional proteins. Therefore we have raised antibodies to representative V2Rs and show labeling of vomeronasal neurons demonstrating that V2R genes encode expressed receptors. V2R immunoreactivity was detected at the sensory surface of the vomeronasal organ in dendritic terminals, indicating that these receptors are capable of directly interacting with pheromones and mediating physiological responses. Immunohistochemistry confirmed that three V2R receptors are expressed in small subsets of sensory neurons. However, surprisingly we found that a subfamily of V2R genes is broadly expressed in the Goα-layer of the vomeronasal organ and are coexpressed in the same cells as other V2Rs. This is in direct contrast to the main olfactory epithelium where sensory neurons express only a single receptor. Thus, our results suggest that different modes of the information processing may occur in the main and accessory olfactory systems.

Mammalian pheromones are thought to be diverse chemical signals that play a role in controlling interactions between individuals of a single species (Keverne, 1983;Novotny et al., 1986; Halpern, 1987; Tirindelli et al., 1998). For example, under specific conditions, pheromones evoke neuroendocrine responses among conspecifics resulting in mating (Keverne, 1983). Thus subtle changes in response to pheromones may provide an important mode of speciation.

The vomeronasal organ (VNO) is a chemosensory organ located at the base of the nasal septum of most terrestrial vertebrates that plays a major role in pheromone responses in many mammalian species (Halpern, 1987). Molecular analysis of VNO neurons has revealed unexpected functional differences between this organ and the main olfactory epithelium (Berghard et al., 1996; Wu et al., 1996). In the VNO, the G-proteins Gαi2 and Gαo are expressed in distinct subsets of mature sensory neurons (Halpern et al., 1995; Berghard and Buck, 1996; Jia and Halpern, 1996). Two large and unrelated families of G-protein-coupled receptors, the V1Rs and V2Rs, are expressed in small subsets of the neurons containing Gαi2 and Gαo, respectively, and may encode pheromone receptors (Dulac and Axel, 1995; Herrada and Dulac, 1997; Matsunami and Buck, 1997; Ryba and Tirindelli, 1997). It has been demonstrated that a V1R (VN6) is found in the sensory microvilli, supporting this idea (Takigami et al., 1999). Moreover, the pattern of cellular activation of VNO neurons in response to pheromones parallels the expression pattern of receptors (Holy et al., 2000; Leinders-Zufall et al., 2000). However, there is no direct evidence that any of these molecules are pheromone receptors.

The V2Rs are homologous to the extracellular Ca-sensing receptors (Brown et al., 1993), metabotropic glutamate receptors (Nakanishi, 1992), taste receptors (Hoon et al., 1999), and a family of fish receptors that also appear to play chemosensory role (Cao et al., 1998;Naito et al., 1998; Speca et al., 1999). All these receptors possess a large N-terminal extracellular domain that is likely to form the ligand-binding site (Nakanishi, 1992; Brown et al., 1993). The ligand specificity of one of these receptors (receptor 5.24) from goldfish olfactory epithelium was recently reported (Speca et al., 1999). This receptor is activated primarily by the basic amino acids, arginine and lysine, which are odorants for the fish. One intriguing facet of the expression of receptor 5.24 is that it is widely expressed in the olfactory epithelium. In contrast, many other receptors are expressed in small subsets of cells (Cao et al., 1998; Naito et al., 1998; Speca et al., 1999).

We raised antibodies to several V2Rs to examine expression of these proteins and to investigate their cellular distribution. Immunohistochemistry demonstrates that V2R genes encode proteins that are expressed in the VNO and are likely to function as pheromone receptors. Surprisingly, we observed that V2R2 is expressed at a lower level in almost all the VNO neurons of the Gαo-positive layer, suggesting that cellular responses to pheromones may involve the interaction between receptors of the same family.

MATERIALS AND METHODS

Isolation and expression of the VNO receptors. Escherichia coli expression systems were used to produce peptides encoding part of the extracellular domain of V2Rs. A fragment of the mouse receptor, V2R2 (bases 1053–1807), was subcloned in pET28 (Novagen, Madison, WI). The equivalent region of the extracellular domains of other V2Rs: mouse V2R1 (991–1669), rat Go-VN1 (986–1738), rat Go-VN2 (975–1730), rat Go-VN3 (1577–2332) and rat Go-VN4 (1110–1868) (Herrada and Dulac, 1997; Ryba and Tirindelli, 1997), were amplified from cDNA, sequenced and subcloned in the plasmid pTrcHis2 (Invitrogen, San Diego, CA). The untranslated region of three distinct members of the V2R2 subfamily were amplified and subcloned in pCRII. A fragment encoding the rat homolog of the human receptor related to goldfish receptor 5.24 was generated by degenerate PCR of genomic DNA using primers preceding the first and sixth transmembrane helices. Peptide expression was induced with isopropylthiogalactoside according to standard methods (Invitrogen; Novagen). Bacterial pellets were resuspended in 10 mm Tris, 150 mm NaCl, and 1 mm PMSF and sonicated for 1 min. After centrifugation, pellets were dissolved in 6m guanidinum-HCl in resuspension buffer. Purification of the peptide was performed by affinity chromatography onto a Talon metal affinity resin (Clontech, Palo Alto, CA) according to the manufacturer's instruction. Approximately 2–4 mg was obtained from 200 ml culture.

For Southern hybridization, a fragment of mouse V2R2 corresponding to a single extracellular exon (540–1388) and the 3′ nontranslated region of rat V2R2, V2R2a, and V2R2b were amplified by PCR. Southern blots were washed at high stringency (1 hr at 65°C in 0.1× SSC for the extracellular probe and 20 min at 65°C in 0.5× SSC for the 3′ nontranslated region probes). The rat cDNA library was screened at moderate stringency with a probe to V2R2 (filters were washed at 65°C in 1× SSC).

Antibody generation and Western blotting. The V2R extracellular domain fragments were extensively dialyzed against PBS, and the precipitate that formed was used to immunize rabbits (500–1000 μg each injection). Antibody purification was performed by ammonium sulfate precipitation followed by DEAE exclusion chromatography (Harlow and Lane, 1988). Because the fusion proteins all contained hexahistidine tags, antibodies were preabsorped with a saturated solution of polyhistidine to reduce cross-reactivity. Antibodies were used at a concentration of 20 ng/ml for Go-VN2, 45 ng/ml for Go-VN3, 4 ng/ml for Go-VN4, and 1–20 ng/ml for V2R2. Antibodies were assayed by Western blot analysis of crude plasma membrane preparation from rat VNO and control tissues (Tirindelli and Ryba, 1996).

In situ hybridization and immunohistochemistry. Tissue was obtained from adult Wistar rats and C57BL/6 mice. Frozen sections were cut at 14 μm and attached to silanized slides. Probe preparation andin situ procedures were essentially as described previously (Ryba and Tirindelli, 1997). Riboprobes were labeled with digoxigenin, and signal was developed using an alkaline phosphatase-conjugated antibody and chromogenic substrate. For double-label fluorescent detection, probes were labeled with fluorescein or with digoxigenin. An alkaline–phosphatase-conjugated anti-fluorescein antibody (Amersham Pharmacia Biotech, UK) and a horseradish-peroxidase conjugated anti-digoxigenin antibody were used in combination with fast red and tyramide fluorogenic substrates (Boehringer Mannheim, Indianapolis, IN; New England Nuclear, Boston, MA). Confocal images were obtained with a Leica (Nussloch, Germany) TSC confocal microscope using an argon–krypton laser; 1 μm optical sections were recorded to ensure that any overlapping signal originated from single cells.

For immunohistochemistry, sections were prepared as for the in situ hybridization, blocked in 1% albumin and 0.3% Triton X-100 (blocking solution) for 20 min and incubated with the anti-V2R2 antibody in blocking solution. For double-label immunohistochemistry, anti-V2R2 antibody was labeled with N-hydroxysuccinimide biotin (Sigma, St. Louis, MO) at a ratio of 5:1. Sections were first incubated with the anti-VN antibody and developed with an anti-rabbit IgG conjugated with Alexa-586. After blocking with normal rabbit serum, sections were incubated with 20 ng/ml biotinylated anti-V2R2 antibody (Harlow and Lane, 1988) in presence of 10% normal rabbit serum, and immune complexes were visualized with fluorescein avidin (Vector Laboratories, Burlingame, CA). For preabsoption controls, 5 μg of anti-V2R2 antibody was incubated with 10 μg of the polypeptide against which it was raised or a mix of 10 μg of each of the other V2R polypeptides. Fluorescent images were obtained using a Zeissfluorescent microscope and a Leica TSC confocal microscope equipped with an argon–krypton laser.

RESULTS

We grouped V2Rs according to their sequence conservation and immunized rabbits with expressed extracellular domains of representative V2R genes that encode members of distinct subfamilies. Antibodies against the N-terminal extracellular domain of three V2R-family receptors: Go-VN2, Go-VN3, and Go-VN4 labeled small subsets of VNO neurons (Fig. 1). For all the V2R antibodies, strongest immunoreactivity was in the cell body of VNO neurons, and as indicated in Figure 1d, expression of receptors was detectable in the sensory dendrites extending to the surface of the epithelium. In contrast, no specific immunostaining was observed in the axon bundles and accessory olfactory bulb of either adult or neonatal animals (data not shown).

Fig. 1.
Fig. 1.

Cellular distribution of the V2Rs. Representative rat V2Rs: Go-VN2 (a), Go-VN3 (b), and Go-VN4 (c) were studied by immunohistochemistry in coronal sections of rat VNO; strong staining of a small subset of VNO neurons was observed; dotted lines indicate the basal and luminal edge of the sensory epithelium. The number of antibody staining cells was quantitated and compared with in situ hybridization of corresponding V2R probes by counting positive cells in double-labeled sections. Similar numbers of cells were detected with antibodies and riboprobes for Go-VN2 and Go-VN3, but antibodies to Go-VN4 detected 4172 cells in 40 sections (4 rats), whereas only 1560 cells were positive by in situ hybridization. High magnification of the luminal region of the VNO stained with the anti-VN4 antibody (d) highlights labeling of sensory dendrites and knobs;arrows indicate immunopositive knobs and dendrites on the surface of the VNO epithelium. Scale bars: a–c, 100 μm; d, 25 μm.

The pattern of immunoreactivity observed with an antibody to V2R2 was markedly distinct. Unlike the punctate pattern of expression observed for the other V2Rs that we examined, the antibody to V2R2 labeled all neurons in the basal half of the sensory cell layer of the VNO (Fig.2a,b). The cellular distribution of V2R2 was very similar to that of other V2Rs with prominent labeling of soma (Fig. 2a,b) and sensory microvilli (Fig. 2c) but no labeling of the axon bundles or accessory bulb (data not shown; Fig. 2d). To rule out the possibility that this antibody recognized a truncated receptor or other proteins expressed in a large subset of neurons, we performed Western analysis. As expected, a single specific band was detected in the VNO membranes at ∼100 kDa, the size predicted for the V2R2 protein (Fig.2d). No V2R2 immunoreactivity was detected in membranes from the olfactory bulb, confirming the results of immunohistochemistry or in membranes from a number of other tissues (Fig. 2d). This does not rule out the possibility that the V2R2 antibody recognizes other V2Rs. Therefore, the specificity of the antibodies was examined using Western blots of the fusion proteins against which they were raised. No cross- reactivity was detected (Fig.3a). Moreover, preincubation of the V2R2 antibody with the polypeptide against which it was raised completely blocked immunodetection (Fig. 3b). In contrast, preabsorption of the anti-V2R2 antibody with a mix of fusion proteins to other V2Rs resulted in no difference in the pattern or intensity of immunofluorescence (Fig. 3c). Preabsorption of other V2R antibodies with the fusion protein against which they were raised also abolished staining. However, incubation of these antibodies with the V2R2-fusion protein had no effect on the pattern or number of reactive cells (Table 1).

Fig. 2.
Fig. 2.

V2R2 is expressed in the cell bodies and sensory dendrites of a large subset of VNO neurons. Anti-mouse V2R2 stained all neurons in the basal half of the VNO epithelium in mouse (a) and rat (b);c, higher magnification of the luminal region ofb showing staining of dendrites and knobs. Scale bars:a, 100 μm; b, 30 μm;c, 4 μm. d, Western blot of rat membrane protein extracts stained for anti-V2R2 immunoreactivity shows that this antibody recognizes an ∼100 kDa protein that is expressed in the VNO, but not MOE, olfactory bulb (OB), spleen (SP), or testis (TE); the position of the 94 kDa marker is indicated.

Fig. 3.
Fig. 3.

Specificity of antibodies to V2Rs.a, Western analysis of the four V2R-family antigens using the four antibodies; each antibody only recognizes the polypeptide against which it was raised. b, c, To demonstrate specificity of immunohistochemistry, the anti-V2R2 antibody was incubated with the polypeptide against which it was raised (b) or a mix of three polypeptides encoding the same region of Go-VN2, Go-VN3, and Go-VN4 (c). Preincubation with the V2R2 antigen abolished immunostaining (b), although preincubation with the other polypeptides had no effect on the pattern of immunohistochemistry (c). Scale bar, 100 μm.

View this table:
Table 1.

Specificity of antibodies

We also compared the high-stringency in situ hybridization pattern of the coding sequence of a rat V2R2 with the expression pattern detected with the antibody (Figs. 2b,4a). Very similar distributions of mRNA and antibody staining were observed. We then used Southern analysis to examine whether there are a large number of receptors closely related to V2R2. The presence of several hybridizing bands in genomic digests probed at high stringency with a single exon probe to V2R2 (Fig. 5a) shows that there is a small subfamily of V2R2-related receptors. Screening of a rat VNO cDNA-library identified several receptors that are related in sequence to V2R2. Although these cDNAs were truncated at the 5′-end, preventing analysis of the full coding sequence, they were very closely related to V2R2 (>85% sequence identity within the available coding sequence).

Fig. 4.
Fig. 4.

Several V2R2 subfamily members are co-expressed in the same VNO neurons. In situ hybridizations with digoxigenin-labeled RNA probes to rat V2R2 subfamily members label most neurons in the basal half of the sensory epithelium of rat VNO. Similar patterns of labeling were observed for a coding sequence probe to rat V2R2a (a) and 3′ untranslated regions of rat V2R2a (b), rat V2R2 (c), and rat V2R2b (d). The development time of staining was 12 hr (a) and 60 hr (b–d).

Fig. 5.
Fig. 5.

The V2R2 subfamily and its homology.a, Southern blot analysis of rat genomic DNA cut withEcoRI, BglII, PstI,BamHI, SacI, PvuII, andXbaI and screened at high stringency using a single extracellular exon probe to mouse V2R2 indicates the existence of a family of closely related genes. b, Southern blot analysis of rat genomic DNA cut with EcoRI orPstI and screened at high stringency with PCR products corresponding to the 3′-noncoding region of rat receptors V2R2, V2R2a, and V2R2b indicates that these probes recognize different genes of the V2R2 subfamily. c, The extracellular regions of the full-length rat and mouse V2Rs (V2R, Go-VN, and VR) and related fish-receptors (Ca, GFB, and R) were aligned with ClustalW, and the results were analyzed with Prot-dist and are shown as a neighbor-joining tree. The length of the joining roots indicates the divergence between receptors. Pairwise comparisons between the extracellular domains of V2R2 and other V2Rs yielded values of ∼25% identity; for other V2Rs identity ranged from ∼30% identity e.g., for V2R1 and Go-VN2 to ∼50% identity e.g., for Go-VN2 and Go-VN4.

The divergent 3′-nontranslated regions of three of these receptors were used to examine the expression pattern of individual V2R2 subfamily members in the VNO. The hybridization pattern of all three probes was essentially indistinguishable from that seen with a coding sequence V2R2 probe (Fig. 4). The 3′-nontranslated region probes do not cross- hybridize at the stringency used for in situ hybridization. Indeed genomic Southern analysis indicates that these probes detect different genes, because the sizes of the hybridizing restriction fragments differ (Fig. 5b). Moreover, because two of these probes detect only single bands in genomic Southerns, it seems likely that several distinct but closely related V2R2 subfamily genes are expressed in a large subset of VNO sensory neurons. As expected for probes capable of detecting multiple mRNAs expressed within a single cell, coding sequence probes produced stronger signal (Fig. 4). These data clearly demonstrate that multiple V2R2-related transcripts are expressed in these cells and perhaps contribute to the immunostaining. However, to date, only a single full-length cDNA has been isolated for a V2R2 subfamily member. Therefore we cannot rule out the possibility that these neurons express multiple V2R2 pseudogenes together with a single V2R2 receptor protein.

To unambiguously demonstrate co-expression of V2R receptors, we performed double-label immunohistochemistry experiments and analyzed 1-μm-thick optical sections using confocal microscopy. As expected, almost all the neurons expressing the V2R family receptors: Go-VN2, Go-VN3, and Go-VN4, also contained V2R2 (Fig.6). This was observed along the entire length of the VNO and also was found for the apically located neurons that expressed Go-VN2 in male rats. We confirmed these results usingin situ hybridization double labeling (Fig. 6). Therefore our results strongly suggest that many Gαo-positive neurons express more than one V2R receptor.

Fig. 6.
Fig. 6.

Co-expression of V2Rs in neurons of the VNO epithelium. Double-label immunohistochemistry and in situ hybridization directly demonstrates co-expression of V2Rs in rat VNO neurons. a–c, Images show double labeling with anti-V2R2 (green) and anti-Go-VN2 (a), anti-Go-VN3 (b), and anti-Go-VN4 (c) (red). Scale bar, 100 μm. Higher magnification image (Scale bar, 50 μm) of double label in situ hybridization (d) of V2R2 (red) and rat Go-VN3 (green) also shows co-expression.

DISCUSSION

V2R expression in VNO sensory neurons

Recently it was shown that members of large family of putative pheromone receptors (V2Rs) are expressed in distinct subsets of VNO sensory neurons (Herrada and Dulac, 1997; Matsunami and Buck, 1997;Ryba and Tirindelli, 1997). However, the sequence of several V2R cDNAs indicated that many of these putative receptors might be expressed pseudogenes (Herrada and Dulac, 1997; Matsunami and Buck, 1997). Moreover, in the absence of functional data, other roles for these proteins (for example in directing axon projection) must also be considered (Belluscio et al., 1999; Rodriguez et al., 1999). Therefore we raised antibodies to investigate whether V2Rs are expressed proteins and to examine their distribution in VNO neurons.

The expression pattern of the V2R proteins revealed by the antibody staining was remarkably similar to that obtained by in situhybridization experiments (data not shown; Herrada and Dulac, 1997). For instance, anti-Go-VN3 antibodies labeled neurons localized to the basal layer of the Gαo-positive zone, whereas anti-VN2 antibodies labeled cells extending to the apical region of the VNO. The number of neurons stained by the anti-Go-VN4 antibodies was approximately three times greater than those identified by in situ hybridization with a corresponding cRNA probe, suggesting that this antibody may recognize an epitope found in more than one V2R. However, control experiments using high concentrations of the expressed N-terminal domains of four representative V2Rs showed that the antibodies are specific within this group only recognizing the protein against which they were raised (Fig. 3).

Expression of receptors was detected in the sensory dendrites extending to the surface of the epithelium, consistent with a role for V2Rs as pheromone receptors (Figs. 1, 2). Nonetheless, the high degree of cellular staining is surprising for sensory receptors that might be expected to be concentrated at the luminal surface of the VNO. One possibility is that this distribution might reflect the fast turnover of the receptors exposed to the lumen of the VNO. A second potential role of the V2Rs might be in directing axon guidance (for example, seeBelluscio et al., 1999; Rodriguez et al., 1999). However, the lack of immunoreactivity in the olfactory bulb (Fig. 2d), while not ruling out a role of these receptors in axon guidance mechanisms, suggests any role of V2Rs in axon guidance may be indirect.

V2R2s are co-expressed with other V2Rs

Both immunohistochemistry and in situ hybridization show that the expression pattern of V2R2 and closely related subfamily members is remarkably different from that of other V2Rs. In earlier studies (Ryba and Tirindelli, 1997), we mistakenly overlooked this difference because the weak signal obtained using 3′-nontranslated region probes to V2R2 requires significantly longer development times than the signal generated by other V2R probes. Thus, after short development times, only a few weakly staining scattered cells were detected (Ryba and Tirindelli, 1997). This weak staining suggests that cellular expression of any single V2R2 subfamily member is significantly lower than that of other V2Rs. However, in contrast to the small subsets of neurons that express most V2Rs, all neurons in the basal half of the sensory cell layer of the VNO appear to express several transcripts from the V2R2 subfamily (Figs. 2, 4, 5). Moreover, double-labeling experiments clearly demonstrate co-expression of several different V2Rs in V2R2 containing sensory neurons (Fig. 6).

These differences in expression raise the possibility that V2R2 plays a distinct role from other V2Rs. Sequence comparison of V2Rs also demonstrates that V2R2 is a divergent member of this family (Fig. 5) and places V2R2 closer to fish olfactory receptors than to other V2Rs. This may suggest closer functional similarity between V2R2 and these fish receptors, but equally may reflect the fact that only a small subset of V2R genes has been sequenced. The expression of V2R2 is restricted to the VNO and specifically to the Gαo-expressing cells within this neurosensory epithelium (Herrada and Dulac, 1997; Matsunami and Buck, 1997; Ryba and Tirindelli, 1997). Moreover, the cellular distribution of immunoreactivity is very similar to that seen with antibodies to other V2Rs (Figs. 1, 2). Finally, as is the case for the other V2Rs, only a V2R2 pseudogene is found in the human high-throughput genomic sequences database. Therefore it appears that like the other V2Rs, V2R2 is a chemosensory receptor that probably plays a role in mediating pheromone responses.

The V2R-related family of receptors from fish olfactory epithelia also contains receptors that are expressed in a small subset of neurons and others that are more broadly expressed (Cao et al., 1998; Naito et al., 1998; Speca et al., 1999). Specifically, it has been shown that two goldfish receptors, 5.24 and 5.3, are expressed in a large subset of olfactory neurons. Thus here too, it is likely that receptors of this family are co-expressed in the same cells. Again it is notable that receptors 5.24 and 5.3 have the most divergent sequences (Speca et al., 1999; Fig. 5). Receptor 5.24 has been shown to respond to arginine and lysine, which are important odorant cues for fish. However, receptor 5.24 is only distantly related to V2R2, and therefore it is unlikely that V2R2 responds with similar ligand specificity.

What might be the functional significance of expressing multiple receptors in a single neuron? In the MOE, neurons express a single receptor, and neurons expressing a common receptor project to the same set of glomeruli (for review, see Mombaerts et al., 1996). Interestingly, olfactory receptors can detect more than one odorant, and odorants can be recognized by several receptors (Malnic et al., 1999). Thus, mammals use combinatorial codes of glomeruli activation to discriminate odors (Rubin and Katz, 1999). The apical neurons of the rodent VNO are also thought to express only a single sensory receptor, however neurons expressing single V1Rs target multiple glomeruli (Belluscio et al., 1999; Rodriguez et al., 1999). Moreover, glomeruli appear to receive input from neurons expressing distinct V1Rs (Belluscio et al., 1999). This slightly different organization may facilitate stereotyped responses to specific mixes of odorants.Caenorhabditis elegans has a much simpler nervous system and uses a very different strategy that permits detection of many odorants but more limited discrimination between them. To do this, C. elegans chemosensory neurons express many receptors per cell (Troemel et al., 1995). Interestingly, the uniform bitter taste of many toxins also appears to result from the co-expression of several distantly related receptors in mammalian taste receptor cells (Adler et al., 2000; Chandrashekar et al., 2000). The co-expression of V2R2 with another V2R receptor suggests that yet another mode of chemosensory information processing may occur. For example it is possible that ligands for V2R2 significantly alter the effects mediated by ligands to other V2Rs, either by sensitizing or desensitizing the cells. It is even conceivable that ligands for V2R2 might over-ride signals mediated by other V2Rs. However, a recent study of the effects of urine on VNO neurons provided no evidence for such an extreme model (Holy et al., 2000). An alternative hypothesis is that V2R2 might form heterodimers with other V2Rs. For example it has been shown that GABA-b receptors are composed of two distinct but related G-protein-coupled receptors (Jones et al., 1998; Kaupmann et al., 1998; White et al., 1998). InDrosophila the pattern of receptor expression most closely parallels the V2R receptor expression profile. Sensory neurons each express a single member of a family of ∼50 odorant receptors (Vosshall et al., 2000), and in addition all express a highly divergent member of this gene family.

In conclusion, our data suggest that V2R2 and the other V2Rs have similar cellular localization and are present in the sensory region of the neurons, as would be expected for receptors involved in pheromone detection. However, the expression pattern of V2Rs is significantly more complex than for other vertebrate olfactory receptors. It will be fascinating to determine how these patterns of expression are regulated and to determine their role in chemosensory signaling and pheromone responses.

Footnotes

  • This research was supported by the Italian Ministero dell'Universita' e della Ricerca Scientifica e Tecnologica. New sequences of V2R2-subfamily members have been deposited in GenBank with accession numbers AF318939 and AF318940. We thank Dr. R. Percudani for helpful discussion.

    Correspondence should be addressed to Roberto Tirindelli, Istituto di Fisiologia Umana, Via Volturno, 39, I-43100, Parma, Italy. E-mail:robertin{at}unipr.it.

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Co-Expression of Putative Pheromone Receptors in the Sensory Neurons of the Vomeronasal Organ
Sara Martini, Lucia Silvotti, Arild Shirazi, Nicholas J. P. Ryba, Roberto Tirindelli
Journal of Neuroscience 1 February 2001, 21 (3) 843-848; DOI: 10.1523/JNEUROSCI.21-03-00843.2001
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