Fig. 3. Retinal layer thickness and ganglion cell density at P17 (n = 6 per group). A, INL thickness of the retina. B, Differential retinal layer thickness analysis of the central retina (450–750 μm).C, Ganglion cell density in the central retina (450–750 μm). Room-air-raised control mice, White circles(A) and white columns(B, C); eyes of iNOS(+/+) mice of the ischemic phase of the experiment, white squares (A) andhatched columns (B, C); eyes of iNOS(−/−) mice of the ischemic phase of the experiment, black triangles(A) and black columns(B, C). A, Quantification of the INL at P17 revealed a generalized thinning of the INL in eyes of iNOS(+/+) and iNOS(−/−) mice of the ischemic phase of the experiment compared with room-air-raised control mice. More striking and highly significant differences in the thickness of the INL were observed in the central area (600 μm distance from nerve head), which corresponds to the ischemic area at P14 in post-oxygen-incubated animals. For A andB, * indicates significant differences by the Mann–Whitney test: p = 0.0001, post-oxygen-incubated iNOS(+/+) mice compared with room-air-raised animals; p = 0.0079, post-oxygen-incubated iNOS(−/−) mice compared with room-air-raised animals; p = 0.0016, post-oxygen-incubated iNOS(+/+) mice compared with post-oxygen-incubated iNOS(−/−) mice. B, In the central area (450–750 μm), additionally to the INL thinning, a significant thinning of the IPL can be observed. ** indicates significant differences by the Mann–Whitney test: p = 0.0020, post-oxygen-incubated iNOS(+/+) mice compared with room-air-raised animals; p = 0.0089, post-oxygen-incubated iNOS(−/−) mice compared with room-air-raised animals; p = 0.0074, post-oxygen-incubated iNOS(+/+) mice compared with post-oxygen-incubated iNOS(−/−) mice.C, Analysis of ganglion cell density showed no alteration in any of the groups.