Fig. 1. Immunoblot of Nogo-A and NgR expression in mouse tissues. A, Samples (20 μg) from the indicated tissues from mice of the indicated ages were analyzed by anti-Nogo-A immunoblot. D, Dorsal root ganglion; B, brain; S, spinal cord; M, skeletal muscle; H, heart; K, kidney;LU, lung; L, liver. The migration of recombinant Nogo-A is shown at right with molecular weight standards of 233, 135, and 112 kDa. B, Immunoblot for NgR in adult mouse brain (B), adult liver (L), NgR-expressing COS cells (COS, +), or control COS cells (COS, −). Molecular weight standards are indicated at the right, in kilodaltons.