Article Figures & Data
Figures
- Fig. 1.
Alkaline phosphatase staining of virus-infected newly generated cells in the GCL of the olfactory bulb. Photomicrographs (left and center columns) and camera lucida drawings (right column) show examples of stained developing granule neurons at different maturation stages as defined in Results: class 1 (A–C); class 2 (D–F); class 3 (G–I); class 4 (J–L); class 5 (M–O). Scale bars, 25 μm.
- Fig. 2.
Alkaline phosphatase staining of the dendritic arbors of virus-infected newly generated cells in EPL of the olfactory bulb. Representative photomicrographs show the dendritic arborization of developing granule neurons at early stages of maturation (A) and at later stages, after developing dendritic spines (B). Scale bars, 25 μm.
- Fig. 3.
Time course of the development of adult-generated olfactory bulb granule neurons. Percentage of alkaline phosphatase-labeled cells in the GCL of the olfactory bulb belonging to each of the developing stages defined in Results (Fig. 1) at different survival times. Top diagram indicates an example of each morphological class. EPL, External plexiform layer; MCL, mitral cell layer;GCL, granule cell layer; RMS, rostral migratory stream.
- Fig. 4.
Labeled cells in the GCL of the olfactory bulb at different survival points after [3H]thymidine injection. A, Photomicrograph of a [3H]thymidine-positive, NeuN-positive cell in the GCL. Scale bar, 20 μm. B, The number of [3H]thymidine-labeled cells in the GCL at different survival times. C, The volume of the GCL at different survival times. Values indicate mean ± SE.
- Fig. 5.
Morphology and cell death in the olfactory bulbs of anosmic mice. Photomicrographs of a Nissl-stained coronal section of the olfactory bulb of an anosmic mouse (A) or a control littermate (B). Dotted lines outline the contour of the RMS. Scale bars, 0.5 mm. C, Volume of the GCL in anosmic (n = 3) and control mice (n = 4). Representative photomicrographs show TUNEL-stained apoptotic cells in the GCL of anosmic (D) and control mice (E). Scale bars, 50 μm. E, Quantification of TUNEL staining in the GCL of anosmic (KO) (n = 3) and control mice (wt) (n = 4). Values indicate mean ± SE. Statistical analysis was conducted by unpaired Student's t test. Significance was established at **p < 0.01.
- Fig. 6.
Morphology and cell survival of newly generated cells in the olfactory bulb of anosmic mice. A, Photomicrograph of a class 4 cell in the GCL of an anosmic mouse. Several focal planes were combined in Photoshop to reconstruct the cell. Scale bar, 50 μm. B, Photomicrograph of the dendritic tree of a class 5 cell in the EPL of an anosmic mouse. Scale bar, 20 μm. C, The percentage of cells belonging to each of the five morphological classes in anosmic (KO) and control littermates (wt). D, Total number of [3H]thymidine- and BrdU-labeled cells in the GCL in control (n = 4) or anosmic mice (n = 3). Statistical analysis was conducted by unpaired Student's t test. Significance was established at *p < 0.05. Time line represents the injection protocol for the experiment. Arrowsindicate injection of either BrdU or [3H]thymidine. X indicates time of perfusion. Values indicate mean ± SE.
Tables
Days after injection % of labeled cells NeuN + % of NeuN + cells labeled 15 68.8 ± 2.3 2.2 ± 0.07 22 80.2 ± 3.8 2.1 ± 0.04 30 81.2 ± 2 1.7 ± 0.13 45 84.1 ± 3.9 1.5 ± 0.03 75 84.3 ± 1 1.4 ± 0.11 365 81.9 ± 3.2 0.3 ± 0.07 Values are ± SEM.
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