ARTICLE, Behavioral/Systems

New Intrathalamic Pathways Allowing Modality-Related and Cross-Modality Switching in the Dorsal Thalamus

John W. Crabtree and John T. R. Isaac
Journal of Neuroscience 1 October 2002, 22 (19) 8754-8761; https://doi.org/10.1523/JNEUROSCI.22-19-08754.2002

Abstract

Transmission through the dorsal thalamus involves nuclei that convey different aspects of sensory or motor information. Cells in the dorsal thalamus are strongly inhibited by the GABAergic cells of the thalamic reticular nucleus (TRN). Here we show that stimulation of cells in specific dorsal thalamic nuclei evokes robust IPSCs or IPSPs in other specific dorsal thalamic nuclei and vice versa. These IPSCs are GABAA receptor-mediated currents and are consistent with the activation of disynaptic intrathalamic pathways mediated by TRN. Thus, cells engaged in sensory analyses in the ventrobasal complex or the medial division of the posterior complex can interact with cells responsive to sensory events in the caudal intralaminar nuclei, whereas cells engaged in motor analyses in the ventrolateral nucleus can interact with cells responsive to motor events in the rostral intralaminar nuclei. Furthermore, sensory event-related cells in the caudal intralaminar nuclei can interact with motor event-related cells in the rostral intralaminar nuclei. In addition, single cells in one dorsal thalamic nucleus can receive convergent inhibitory inputs after stimulation of cells in two or more other dorsal thalamic nuclei, and TRN-mediated inhibitory inputs can momentarily switch off tonic firing of action potentials in dorsal thalamic cells. Our findings provide the first direct evidence for a rich network of intrathalamic pathways that allows modality-related and cross-modality inhibitory modulation between dorsal thalamic nuclei. Moreover, TRN-mediated switching between dorsal thalamic nuclei could provide a mechanism for the selection of competing transmissions of sensory and/or motor information through the dorsal thalamus.

The dorsal thalamus is usually thought of as a large collection of nuclei in which each nucleus has its own separate channels through which to transmit sensory or motor information. Such transmission involves first-order nuclei, which receive messages about the sensory or motor periphery through ascending pathways, and higher-order nuclei, which receive messages about cortical processing through descending pathways from cortical layer V (Guillery, 1995; Guillery et al., 1998; Sherman and Guillery, 2001). Both first- and higher-order nuclei project to the cortex, completing corticopetal and corticothalamocortical pathways, respectively. We can tentatively add to this scheme nuclei that contain a mixture of first- and higher-order circuits (Jones, 1985; Macchi and Bentivoglio, 1986;Deschênes et al., 1994; Guillery, 1995; Sherman and Guillery, 2001). These mixed-circuit nuclei are the intralaminar nuclei, which project to both the striatum and cortex (Jones, 1985).

Neurons in the thalamic reticular nucleus (TRN) play a major role in modulating the flow of information through the dorsal thalamus (Sherman and Koch, 1986; Sherman and Guillery, 1996, 2001; Guillery et al., 1998; Crabtree, 1999). These cells are part of the ventral thalamus (Rose, 1942), are GABAergic (Houser et al., 1980), and can exert powerful inhibitory effects on dorsal thalamic cells (Salt, 1989; Lee et al., 1994a,b; Warren and Jones, 1994; Cox et al., 1997;Kim and McCormick, 1998). Each dorsal thalamic nucleus is reciprocally connected to TRN (Scheibel and Scheibel, 1966; Jones, 1975, 1985;Guillery et al., 1998; Crabtree, 1999), and these disynaptic pathways form closed- or open-loop circuits (Shosaku, 1986; Lo and Sherman, 1994; Pinault and Deschênes, 1998a; Crabtree, 1999), providing feedback and lateral inhibition, respectively, in a given dorsal thalamic nucleus.

Previously, we showed TRN-mediated pathways between two somatosensory-related dorsal thalamic nuclei, the ventrobasal complex (VB), a first-order nuclear complex, and the medial division of the posterior complex (POm), a higher-order nucleus (Crabtree et al., 1998;Crabtree, 1999). Cells in VB and POm are topographically organized in that rostrally to progressively more caudally located VB and POm cells can interact, reflecting the relatively parallel trajectories of thalamoreticular and reticulothalamic axons that stream within the thalamus and that relate to particular sectors of TRN (Pinault et al., 1995; Deschênes et al., 1996, 1998; Pinault and Deschênes, 1998b) (see Fig. 1). These axonal trajectories suggest that there may be many more TRN-mediated pathways in the thalamus. For example, the intrathalamic axons pertaining to the caudal intralaminar nuclei (CIL), a mixed-circuit sensory-related nuclear complex (Albe-Fessard and Besson, 1973; Dong et al., 1978; Peschanski et al., 1981; Grunwerg and Krauthamer, 1992; Berkley et al., 1995; Matsumoto et al., 2001;Minamimoto and Kimura, 2002), overlap such axons pertaining to VB and POm. Similarly, the intrathalamic axons pertaining to the rostral intralaminar nuclei (RIL), a mixed-circuit motor-related nuclear complex (Schlag et al., 1974; Schlag-Rey and Schlag, 1977, 1984; Schlag and Schlag-Rey, 1984), overlap such axons pertaining to the ventrolateral nucleus (VL), a first-order motor nucleus (Jones, 1985;Guillery, 1995; Sherman and Guillery, 2001). Furthermore, TRN cells that are connected to the intralaminar nuclei are intermingled and have a relatively widespread distribution (Jones, 1975; Steriade et al., 1984; Kolmac and Mitrofanis, 1997; Lizier et al., 1997). Therefore, one might expect to find various TRN-mediated pathways that would allow a considerable range of modality-related and cross-modality interactions between populations of dorsal thalamic cells that differ enormously with regard to where their driving afferents originate, their receptive field properties, and where their efferents terminate (Jones, 1985;Macchi and Bentivoglio, 1986; Groenewegen and Berendse, 1994; Guillery, 1995; Sherman and Guillery, 1998, 2001).

Using a thalamic slice preparation, we present evidence for TRN-mediated pathways between cells in the following pairs of dorsal thalamic nuclei: VB and CIL, POm and CIL, VL and RIL, and RIL and CIL. Furthermore, activation of cells in two or more dorsal thalamic nuclei can lead to convergent inputs in cells in another dorsal thalamic nucleus. Finally, tonic firing of action potentials in cells in one dorsal thalamic nucleus can be temporarily switched off by activation of cells in another dorsal thalamic nucleus. Our findings provide direct evidence for a specifically organized network of intrathalamic connections and for a functional role for the interactions within this network.

MATERIALS AND METHODS

Slice preparation. All experimental procedures were performed in accordance with United Kingdom Home Office regulations (Animals Scientific Procedures Act of 1986). Thirteen- to 20-d-old Wistar rats were deeply anesthetized with sodium pentobarbital (60 mg/kg, i.p.), and their brains were removed and placed in ice-cold extracellular solution. Horizontal thalamic slices (500 μm thick) were cut on a vibratome and were allowed to recover for at least 1 hr at room temperature (23–25°C). They were then transferred to a recording chamber and submerged beneath continuously superfusing extracellular solution saturated with 95% O2 and 5% CO2. The extracellular solution contained the following (in mm):119 NaCl, 2.5 KCl, 1.0 NaH2PO4, 26.2 NaHCO3, and 11.0 glucose, pH 7.4.

Electrophysiology. Whole-cell voltage- and/or current-clamp recordings were made from neurons in VB, POm, VL, CIL, and RIL using 3–5 MΩ glass electrodes. For voltage-clamp recordings, the whole-cell solution contained the following (in mm): 135 Cs methane sulfonate, 10.0 HEPES, 0.5 EGTA, 3.0 NaCl, 5.0 QX-314Cl, 4.0 Mg-ATP, 0.3 Na-GTP, and 10.7 biocytin, pH 7.2 with CsOH (275 mOsm). For current-clamp recordings, the whole-cell solution contained the following (in mm): 130 K methane sulfonate, 5.0 HEPES, 0.2 EGTA, 8.5 NaCl, 4.0 Mg-ATP, 0.5 Na-GTP, and 10.7 biocytin, pH 7.2 with 50% KOH (275 mOsm). During recordings, cells were held at −40 mV (voltage-clamp recordings) or close to −55 mV (current-clamp recordings). A glass electrode (2.5–3.0 μm tip diameter) was placed in one of the above nuclei and was used to evoke intrathalamic responses in another of the above nuclei. This stimulating electrode was filled with 2 mml-glutamate monosodium salt in extracellular solution, pH 7.4, and 5.0% methylene blue and was connected to a pressure injection unit. Glutamate was ejected by pulses of pressure (5–15 psi; 50 msec duration) at a frequency of 0.05 Hz. When an optimal evoked response was obtained, stimulation was maintained uninterrupted at a constant frequency and intensity for the duration of the experiment.

Recordings were made using an Axopatch-200B amplifier (Axon Instruments, Foster City, CA). Data were filtered at 5 kHz, digitalized at 10 kHz, and stored on computer. IPSC amplitudes, input resistance, and series resistance were displayed and analyzed on-line using custom software (Anderson and Collingridge, 2001). IPSC peak amplitudes were measured by averaging across a 25–30 msec window centered on the peak of the response. Data are expressed as percentages of the average baseline amplitude (baseline of 100%). Pooled data are expressed as means ± SEM.

Histology and reconstructions. During each recording, the location of the stimulation site, as indicated by the ejected blue dye, and the location of the recording site were drawn on a standardized map of the dorsal thalamus (see Fig. 1). After recordings tracer-filled neurons were processed for biocytin histochemistry using the ABC–diaminobenzidine method (Horikawa and Armstrong, 1988), and the locations of the cells in the dorsal thalamus were confirmed by Nissl counterstaining with cresyl violet.

RESULTS

Visualization and stimulation of thalamic nuclei

An in vitro slice preparation (Crabtree et al., 1998) was used to make whole-cell voltage- or current-clamp recordings from neurons in VB, POm, VL, CIL (including the caudal parts of the centrolateral and paracentral nuclei and the parafascicular nucleus), and RIL (including the rostral parts of the centrolateral and paracentral nuclei), and the responses of these cells to stimulation of the dorsal thalamus were monitored. By cutting slices in the horizontal plane, intrathalamic connections linking TRN to VB, POm, VL, CIL, and RIL were preserved, whereas connections between the thalamus and the cortex were severed. The slices were transilluminated from below using two orthogonal reflective surfaces, which resulted in contrast interference patterns that clearly revealed the nuclei of the thalamus (Fig. 1) and which enabled the appropriate positioning of the recording and stimulating electrodes. Dorsal thalamic neurons were stimulated using local application of glutamate, which activated cell bodies but not axons of passage (Crabtree et al., 1998). During an experiment, the inclusion of a blue dye in the stimulating solution allowed the location of the stimulation site and the spread of the stimulating solution to be visualized.

Fig. 1.
Fig. 1.

The thalamic slice preparation as it appears in the recording chamber. Transilluminating the slice clearly reveals TRN (R), VB, POm, VL, RIL, CIL, the anteroventral (AV) and mediodorsal (MD) nuclei, and the retroflex fasciculus (asterisk). Thedashed lines indicate the borders of the various nuclei. Rostral is to the top, and medial is to theright. Scale bar, 500 μm.

Response of sensory-related dorsal thalamic cells to dorsal thalamic stimulation

Initially, we examined the possibility that intrathalamic pathways exist between sensory-related dorsal thalamic nuclei besides the pathways between VB and POm (Crabtree et al., 1998; Crabtree, 1999). Robust IPSCs (Fig. 2A) were recorded in seven VB cells (Fig.2D,G) and seven POm cells in response to glutamate stimulation in CIL (Fig. 2G) and in 14 CIL cells in response to glutamate stimulation in VB or POm. Here and elsewhere, these IPSCs had an amplitude that varied between 20 and 120 pA and rose rapidly. The IPSCs were always substantially larger than spontaneous IPSCs, if present, and were evoked with a constant latency typically between 50 and 100 msec relative to the beginning of the glutamate pulse. A proportion of this latency presumably included the time required for the glutamate to reach and activate the appropriately connected dorsal thalamic cells. The presence of these pathways suggests that there can be reciprocal modulation of transmission between sensory-related VB or POm and CIL in addition to the possibility of such modulation between VB and POm.

Fig. 2.
Fig. 2.

Glutamate stimulation in a sensory or motor dorsal thalamic nucleus evokes IPSCs in cells in another sensory or motor dorsal thalamic nucleus. A, Example of an IPSC recorded from a VB cell in response to glutamate stimulation in CIL (CIL→VB). B, Example of an IPSC recorded from a VL cell in response to glutamate stimulation in RIL (RIL→VL). Calibration also applies to A. C, Example of an IPSC recorded from an RIL cell in response to glutamate stimulation in CIL (CIL→RIL). Glutamate stimulation is indicated by the black bars in A–C. D, The biocytin-filled VB cell from which the recording inA was obtained. E, The biocytin-filled VL cell from which the recording inB was obtained. F, The biocytin-filled RIL cell from which the recording in C was obtained.G, Horizontal section through the thalamus showing the locations of the recorded cell in VB (black dot) shown in D and the stimulation site in CIL (open circle). H, Horizontal section through the thalamus showing the locations of the recorded cell in VL (black dot) shown in E and the stimulation site in RIL (open circle). I, Horizontal section through the thalamus showing the locations of the recorded cell in RIL (black dot) shown in F and the stimulation site in CIL (open circle). Rostral is to thetop, and medial is to the right(G–I). Scale bars:G–I, 500 μm.

Response of motor-related dorsal thalamic cells to dorsal thalamic stimulation

We next explored the possibility that intrathalamic pathways exist between motor-related dorsal thalamic nuclei. Robust IPSCs (Fig.2B) were recorded in eight VL cells (Fig.2E,H) in response to glutamate stimulation in RIL (Fig. 2H) and in eight RIL cells in response to glutamate stimulation in VL. The existence of these pathways suggests that, in addition to modulation between sensory-related nuclei, there can be reciprocal modulation of transmission between motor-related VL and RIL.

Response of sensory- or motor-related dorsal thalamic cells to dorsal thalamic stimulation

We then examined the possibility that intrathalamic pathways exist between sensory- and motor-related dorsal thalamic nuclei. Robust IPSCs (Fig. 2C) were recorded in eight RIL cells (Fig.2F,I) in response to glutamate stimulation in CIL (Fig. 2I) and in eight CIL cells in response to glutamate stimulation in RIL. The presence of these pathways suggests that, in addition to modulation between sensory-related nuclei and between motor-related nuclei, there can be reciprocal modulation of transmission between sensory-related CIL and motor-related RIL.

After an experiment, recovery of biocytin-filled cells (Figs.2D–F) (see also Fig.5B,D,H) and Nissl counterstaining (Fig. 3A–C) confirmed the locations of recorded cells in VB, POm, VL, CIL, or RIL. For each of the above sensory-, motor-, and sensory- and motor-related pathways, there was a topographic relationship between the location of a responsive cell and the location of the stimulating electrode that evoked the response. That is, stimulating in rostrally to progressively more caudally located sites evoked IPSCs in rostrally to progressively more caudally located cells (Fig.3D). The division of the intralaminar nuclei into two regions (Fig. 1, dashed line) was purely functional in that only cells lying in the region designated CIL interacted with VB or POm cells and only cells lying in the region designated RIL interacted with VL cells. Furthermore, glutamate stimulation never evoked EPSCs in a recorded cell, indicating that there are no direct connections between the nuclei we examined.

Fig. 3.
Fig. 3.

Stimulation and recording sites are topographically organized between dorsal thalamic nuclei.A, Horizontal section through the thalamus showing Nissl staining. The arrow points to the biocytin-filled RIL cell shown in Figure 5D. The black dotsindicate the borders between RIL and VL (left) and RIL and the mediodorsal nuclei (bottom right).B, Horizontal section through the thalamus showing Nissl staining. The arrow points to the biocytin-filled CIL cell shown in Figure 5H. The black dotsindicate the borders between CIL and POm (left) and CIL and the mediodorsal nuclei (top right). Theasterisk indicates the retroflex fasciculus.C, Horizontal section through the thalamus showing Nissl staining. The arrow points to a biocytin-filled VB cell. The black dots indicate the borders between VB and TRN (top left) and VB and VL (top right).D, Horizontal section through the thalamus showing a summary of the topographic relationship between stimulation sites (arrows) and recording sites (arrows) in various dorsal thalamic nuclei. The corresponding directions of thearrows indicate the locations of the stimulating electrode in one dorsal thalamic nucleus and of cells in another dorsal thalamic nucleus in which a response was evoked. Rostral is to thetop, and medial is to the right(A–D). Scale bars: (in B)A, B, 200 μm; C, 200 μm; D, 500 μm.

It is important to note here that, although we routinely attempted to activate them, IPSCs could not be evoked between cells in the following pairs of nuclei: VB and VL, VB and RIL, POm and VL, POm and RIL, and VL and CIL (see Fig. 7). Therefore, cells in these nuclear pairs would not be expected to modulate the transmission of each other. The absence of intrathalamic connections between specific pairs of dorsal thalamic nuclei may provide an important clue as to the function of such connections between other specific pairs of dorsal thalamic nuclei.

Characterization of the response evoked in dorsal thalamic cells

The IPSCs were reversibly antagonized by the competitive GABAA antagonist bicuculline (10 μm). After the collection of a stable baseline of IPSCs, bicuculline was bath applied for 10 min, which blocked the response, and, on washout, the IPSCs returned (Fig.4A). Summary data for 10 cells (Fig. 4B) show that bicuculline caused a reduction in IPSC amplitude to 4 ± 1% of baseline and, on washout, the amplitude recovered to 81 ± 14%.

Fig. 4.
Fig. 4.

Bicuculline blocks the IPSCs evoked in cells in one dorsal thalamic nucleus by glutamate stimulation in another dorsal thalamic nucleus. A, Amplitude of IPSCs, evoked in a CIL cell by glutamate stimulation in VB, versus time from an example experiment in which bicuculline was bath applied (black bar). Top, Individual traces taken at the times indicated (1, 2, 3) during the experiment; glutamate stimulation is indicated by the black bars. B, Summary graph of the effect of bicuculline (black bar) on the evoked IPSCs from 10 experiments in which each pair of interacting dorsal thalamic nuclei is represented at least once. Dashed line indicates the baseline response level (100%) in A andB.

Convergence of inhibitory inputs onto dorsal thalamic cells

The above results together with those from our previous study (Crabtree et al., 1998) suggest that activation of cells in two or more dorsal thalamic nuclei could result in convergent inhibitory inputs onto single cells in another dorsal thalamic nucleus (see Fig. 7). This possibility was examined in eight additional dorsal thalamic cells. IPSCs were initially recorded in a cell in one dorsal thalamic nucleus in response to glutamate stimulation in another dorsal thalamic nucleus. Then the stimulating electrode was repositioned and IPSCs were recorded in the same cell to glutamate stimulation in yet another dorsal thalamic nucleus and so forth. In this manner, IPSCs were recorded in single VB cells in response to sequential glutamate stimulation in POm and CIL, and IPSCs (Fig.5A) were recorded in single POm cells (Fig. 5B,E) in response to sequential glutamate stimulation in VB and CIL (Fig. 5E). Furthermore, IPSCs (Fig. 5C) were recorded in single RIL cells (Fig. 5D,F) in response to sequential glutamate stimulation in VL and CIL (Fig.5F). And finally, IPSCs (Fig. 5G) were recorded in single CIL cells (Fig.5H,I) in response to sequential glutamate stimulation in VB, POm, and RIL (Fig.5I). Such convergence of inhibitory inputs suggests that transmission through single cells in one dorsal thalamic nucleus can be modulated by cells in two or more other dorsal thalamic nuclei.

Fig. 5.
Fig. 5.

Glutamate stimulation in two or more dorsal thalamic nuclei evokes IPSCs in single cells in another dorsal thalamic nucleus. A, Examples of IPSCs recorded from a POm cell in response to glutamate stimulation in VB (VB→POm,top trace) and in CIL (CIL→POm, bottom trace). B, The biocytin-filled POm cell from which the recordings in A were obtained.C, Examples of IPSCs recorded from an RIL cell in response to glutamate stimulation in VL (VL→RIL, top trace) and in CIL (CIL→RIL, bottom trace). Glutamate stimulation is indicated by the black bar(top and bottom traces: A,C). Calibration also applies to A.D, The biocytin-filled RIL cell (see Fig.3A) from which the recordings in C were obtained. E, Horizontal section through the thalamus showing the locations of the recorded cell in POm (black dot) shown in B and the stimulation sites in VB (left open circle) and CIL (right open circle). F, Horizontal section through the thalamus showing the locations of the recorded cell in RIL (black dot) shown inD and the stimulation sites in VL (top open circle) and CIL (bottom open circle).G, Examples of IPSCs recorded from a CIL cell in response to glutamate stimulation in VB (VB→CIL, top trace), in POm (POm→CIL, middle trace), and in RIL (RIL→CIL, bottom trace). Glutamate stimulation is indicated by the black bar (top,middle, and bottom traces).H, The biocytin-filled CIL cell (see Fig.3B) from which the recordings in G were obtained. I, Horizontal section through the thalamus showing the locations of the recorded cell in CIL (black dot) shown in H and the stimulation sites in VB (left open circle), POm (bottom middle open circle), and RIL (top right open circle). Rostral is to the top, and medial is to theright (E, F,I). Scale bars: E,F, I, 500 μm.

Interruption of tonic firing in dorsal thalamic cells

To examine a possible functional role for the intrathalamic pathways, whole-cell recordings were made from 16 additional dorsal thalamic cells and their properties were investigated in both voltage- and current-clamp modes. Each pair of interacting dorsal thalamic nuclei was examined at least once. In a typical experiment, IPSCs (Fig.6A) were initially recorded in voltage-clamp mode in a cell in one dorsal thalamic nucleus (Fig. 6B) in response to glutamate stimulation in another dorsal thalamic nucleus (Fig. 6B). Then, in current-clamp mode, a train of action potentials was evoked in the cell using a depolarizing current pulse (Fig. 6C). When combining the stimulation with the depolarizing current pulse, the glutamate stimulation interrupted the action potentials for ∼100 msec (Fig.6D). The functional implication of this interruption of action potentials is that cells in interacting pairs of dorsal thalamic nuclei can temporarily switch off the activity of each other.

Fig. 6.
Fig. 6.

Glutamate stimulation in one dorsal thalamic nucleus interrupts trains of action potentials in cells in another dorsal thalamic nucleus. A, An example of an IPSC recorded from an RIL cell in response to glutamate stimulation (black bar) in CIL (CIL→RIL). B, Horizontal section through the thalamus showing the locations of the recorded cell in RIL (black dot) and the stimulation site in CIL (open circle). Rostral is to thetop, and medial is to the right. Scale bar, 500 μm. C, When held close to −55 mV, the RIL cell responded with a train of action potentials to a depolarizing 0.4 nA current pulse (bottom) injected into the cell.D, A train of action potentials in the RIL cell to the depolarizing current pulse was interrupted by glutamate stimulation (black bar) in CIL.

DISCUSSION

A network of intrathalamic pathways

Using electrophysiological recordings in a thalamic slice preparation, we show that glutamate stimulation of sensory-related cells in VB or POm evokes IPSCs in sensory-related cells in CIL and vice versa. Next we show that glutamate stimulation of motor-related cells in VL (or RIL) evokes IPSCs in motor-related cells in RIL (or VL). We then go on to show that glutamate stimulation of sensory-related cells in CIL evokes IPSCs in motor-related cells in RIL and vice versa. These IPSCs are GABAAreceptor-mediated currents, and single cells in one dorsal thalamic nucleus can receive convergent inhibitory inputs after activation of cells in two or more other dorsal thalamic nuclei. Furthermore, the inhibitory inputs can interrupt, or momentarily switch off, tonic firing of action potentials in dorsal thalamic cells. Our data provide strong evidence for a rich network of intrathalamic pathways that allow modality-related and cross-modality modulation of the flow of information through various first-order, higher-order, and mixed-circuit nuclei in the dorsal thalamus.

The thalamic reticular nucleus mediates the intrathalamic pathways

The IPSCs evoked in dorsal thalamic cells can be entirely accounted for by the activation of disynaptic pathways mediated by the GABAergic cells of TRN. The IPSCs cannot be explained by the activation of polysynaptic pathways involving the cerebral cortex or the GABAergic cells of the zona incerta (Oertel et al., 1982; Power et al., 1999) or substantia nigra (MacLeod et al., 1980; Oertel et al., 1982; Kha et al., 2001), because the thalamic slices were cut in a plane that would sever such connections, nor can the activation of disynaptic circuits between a single dorsal thalamic nucleus and TRN (e.g., RIL to TRN to RIL) account for the IPSCs, because the glutamate stimulation sites were always restricted to a dorsal thalamic nucleus other than the one containing the recorded cell. The IPSCs also cannot be explained by the activation of a monosynaptic pathway from TRN to a given dorsal thalamic nucleus, because glutamate stimulation selectively activated cell bodies but not reticulothalamic axons of passage. Furthermore, in those experiments in which the stimulating electrode was placed in VB or VL adjacent to TRN, the stimulation site was always at least 300 μm away from the inner border of TRN but the stimulating solution containing the blue dye never spread >100 μm within the thalamus. Finally, because all of the dorsal thalamic nuclei we studied lack GABAergic interneurons (Houser et al., 1980; Barbaresi et al., 1986;Bentivoglio et al., 1991; Arcelli et al., 1997), cells in TRN are the only intrathalamic source of GABAergic afferents in our slice preparation. The above considerations strongly indicate that the IPSCs recorded in this study resulted from the activation of various TRN-mediated pathways connecting one dorsal thalamic nucleus to another. We saw no evidence for direct glutamatergic pathways between any of the dorsal thalamic nuclei studied here.

The thalamic reticular nucleus allows modality-related and cross-modality modulation

The TRN-mediated pathways shown here and previously (Crabtree et al., 1998; Crabtree, 1999) link together five pairs of dorsal thalamic nuclei (Fig. 7) that are engaged in the transmission of different aspects of sensory or motor information. VB cells have relatively small receptive fields and convey information about mechanical stimulation of the contralateral skin or deep tissues (Jones, 1985). POm cells have larger receptive fields than VB cells and convey information about cortical processing in the ipsilateral somatosensory cortical area 1 (Diamond et al., 1992a,b). Such processing includes inputs from motor cortical area 1 (Porter and White, 1983; Miyashita et al., 1994). CIL cells have extremely large bilateral receptive fields and convey information about sensory events (Albe-Fessard and Besson, 1973; Dong et al., 1978; Peschanski et al., 1981; Grunwerg and Krauthamer, 1992; Berkley et al., 1995; Matsumoto et al., 2001; Minamimoto and Kimura, 2002). These cells can have multimodal receptive fields (somatosensory, auditory, and visual), suitable for transmitting the occurrence of new and potentially interesting sensory events, or can respond to cutaneous or deep noxious stimuli, suitable for transmitting the occurrence of potentially harmful events. VL cells have relatively small motor fields and convey information about proprioceptive stimulation of contralateral deep tissues related to movement around a single joint and often of a single muscle (Strick, 1976; Vitek et al., 1994, 1996). RIL cells have bilateral oculomotor fields or extremely large unilateral receptive fields and convey information about motor events (Schlag et al., 1974;Schlag-Rey and Schlag, 1977, 1984; Schlag and Schlag-Rey, 1984). These cells can respond to anticipated or actual eye movements or can respond to changes in or the sudden appearance of a visual target that elicits eye movements to or fixation of the eyes on the target. Thus, this linkage between the five pairs of nuclei makes ample allowance for a broad range of modality-related and cross-modality modulation of transmission through the dorsal thalamus.

Fig. 7.
Fig. 7.

Schematic drawing showing the intrathalamic pathways described here and previously (Crabtree et al., 1998;Crabtree, 1999). These pathways link together cells (black ovals) in VB and POm, VB and CIL, POm and CIL, CIL and RIL, and RIL and VL through cells (black ovals) in TRN.

Specificity of thalamic reticular-mediated connectivity

The TRN-mediated linkage of five pairs of dorsal thalamic nuclei could be construed as an example of intrathalamic connectivity underlying widespread interactions among cells in the dorsal thalamus. On the contrary, in this same group of dorsal thalamic nuclei, we found no evidence for interactions between cells in five other nuclear pairs (e.g., between VB and VL). Furthermore, for any given pair of interacting nuclei, there is a strict topographic relationship between the locations of stimulation sites and responsive cells (Fig.3D). Such topography suggests that internuclear modulation of transmission could occur simultaneously in multiple homologous regions in pairs of interacting nuclei.

Thalamic reticular-mediated connectivity: closed- or open-loop circuits?

Cells in each pair or trio of interacting dorsal thalamic nuclei could access a single population of TRN cells (Fig. 7). However, more complex patterns of connectivity are possible. For example, cells in each dorsal thalamic nucleus in an interaction could engage a separate population of TRN cells or could engage a population of TRN cells that is partially shared by cells in one or more other dorsal thalamic nuclei. The actual patterns of connectivity of these intrathalamic pathways will determine whether they form internuclear closed-loop circuits, in which a cell in one dorsal thalamic nucleus inhibits and is inhibited by the same cell in another dorsal thalamic nucleus, or internuclear open-loop circuits, in which a cell in one dorsal thalamic nucleus inhibits and is inhibited by different cells in another dorsal thalamic nucleus.

Convergence of thalamic reticular-mediated inputs

After activation of cells in two or more dorsal thalamic nuclei, inhibitory inputs could converge onto separate populations of cells in another dorsal thalamic nucleus. Thus, one nucleus could engage in simultaneous reciprocal modulation with two or more other nuclei. Alternatively, activation of cells in two or more dorsal thalamic nuclei can lead to convergence of inhibitory inputs onto single cells in another dorsal thalamic nucleus (Fig.5A,C,G). The temporal pattern of the activation of these inputs could determine the response mode of the cell (Deschênes et al., 1984; Jahnsen and Llinás, 1984; Sherman, 2001; Sherman and Guillery, 2001). When the cell is at rest, near simultaneous activation of the inputs could generate large and long-lasting IPSPs that would result from GABAA and potentially GABAB(Crunelli and Leresche, 1991; Sanchez-Vives and McCormick, 1997) receptor activation. The resulting prolonged hyperpolarization could be sufficient to de-inactivate the low-threshold T-type Ca2+ channels, thus priming the cell to fire in burst mode to a depolarizing EPSP.

Thalamic reticular-mediated switching: a mechanism for selective attention?

There has been much speculation about a role for TRN in selective attention (Skinner and Yingling, 1977; Scheibel, 1981; Crick, 1984;LaBerge et al., 1992; Guillery et al., 1998). Here we show that activation of cells in one dorsal thalamic nucleus can lead to IPSPs that temporarily switch off tonic firing of action potentials in cells in another dorsal thalamic nucleus (Fig. 6D). This TRN-mediated switching between cells in two dorsal thalamic nuclei could provide a mechanism for selection between competing processes for limited attentional resources (Desimone and Duncan, 1995). That is, depending on the relative salience of messages reaching the dorsal thalamus at any given moment, transmission would be selected through cells in only one of two given homologous regions in a pair of interacting nuclei. This selectivity of transmission could partition transfer of information through different dorsal thalamic nuclei into discrete temporal components, reflecting the ever-changing attentional demands of the external and internal sensory and motor environment. Furthermore, this selection could prevent simultaneous transmission of attentionally incompatible combinations of messages through the dorsal thalamus. Accordingly, our findings predict that (1) nuclei conveying messages about peripheral sensory analyses (e.g., VB) (Jones, 1985) would compete with nuclei conveying messages about cortical sensory analyses (e.g., POm) (Diamond et al., 1992a,b), (2) nuclei conveying messages about sensory (e.g., VB or POm) or motor (e.g., VL) (Strick, 1976; Vitek et al., 1994, 1996) analyses would compete, respectively, with nuclei conveying messages about the occurrence of new sensory (CIL) (Albe-Fessard and Besson, 1973; Dong et al., 1978; Peschanski et al., 1981; Grunwerg and Krauthamer, 1992; Berkley et al., 1995;Matsumoto et al., 2001; Minamimoto and Kimura, 2002) or motor (RIL) (Schlag et al., 1974; Schlag-Rey and Schlag, 1977, 1984; Schlag and Schlag-Rey, 1984) events, and (3) CIL and RIL would compete with each other for the conveyance of messages about new sensory or motor events. Thus, the dorsal thalamus can no longer be thought of as containing nuclei with their own independent channels of communication. Instead, dorsal thalamic nuclei in each of several specific pairs can interact through TRN, temporarily and competitively switching off the transmission of information of each other.

Footnotes

  • This study was supported by The Wellcome Trust (J.W.C., J.T.R.I.). We thank Andy Griffiths for technical assistance and Bill Anderson for supplying the data acquisition software.

  • Correspondence should be addressed to John W. Crabtree, Medical Research Council Centre for Synaptic Plasticity, Department of Anatomy, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. E-mail: j.w.crabtree{at}bristol.ac.uk.

REFERENCES

  1. ↵
    1. Albe-Fessard D,
    2. Besson JM
    (1973) Convergent thalamic and cortical projections—the non-specific system. in Handbook of sensory physiology, Vol II, Somatosensory system, ed Iggo A (Springer, New York), pp 489–560.
  2. ↵
    1. Anderson WW,
    2. Collingridge GL
    (2001) The LTP program: a data acquisition program for on-line analysis of long-term potentiation and other synaptic events. J Neurosci Methods 108:71–83.
    OpenUrlCrossRefPubMed
  3. ↵
    1. Arcelli P,
    2. Frassoni C,
    3. Regondi MC,
    4. De Biasi S,
    5. Spreafico R
    (1997) GABAergic neurons in mammalian thalamus: a marker of thalamic complexity? Brain Res Bull 42:27–37.
    OpenUrlCrossRefPubMed
  4. ↵
    1. Barbaresi P,
    2. Spreafico R,
    3. Frassoni C,
    4. Rustioni A
    (1986) GABAergic neurons are present in the dorsal column nuclei but not in the ventroposterior complex of rats. Brain Res 382:305–326.
    OpenUrlCrossRefPubMed
  5. ↵
    1. Bentivoglio M,
    2. Spreafico R,
    3. Minciacchi D,
    4. Macchi G
    (1991) GABAergic interneurons and neuropil of the intralaminar thalamus: an immunohistochemical study in the rat and the cat, with notes in the monkey. Exp Brain Res 87:85–95.
    OpenUrlPubMed
  6. ↵
    1. Berkley KJ,
    2. Benoist J-M,
    3. Gautron M,
    4. Guilbaud G
    (1995) Responses of neurons in the caudal intralaminar thalamic complex of the rat to stimulation of the uterus, vagina, cervix, colon, and skin. Brain Res 695:92–95.
    OpenUrlCrossRefPubMed
  7. ↵
    1. Cox CL,
    2. Huguenard JR,
    3. Prince DA
    (1997) Nucleus reticularis neurons mediate diverse inhibitory effects in thalamus. Proc Natl Acad Sci USA 94:8854–8859.
    OpenUrlAbstract/FREE Full Text
  8. ↵
    1. Crabtree JW
    (1999) Intrathalamic sensory connections mediated by the thalamic reticular nucleus. Cell Mol Life Sci 56:683–700.
    OpenUrlCrossRefPubMed
  9. ↵
    1. Crabtree JW,
    2. Collingridge GL,
    3. Isaac JTR
    (1998) A new intrathalamic pathway linking modality-related nuclei in the dorsal thalamus. Nat Neurosci 1:389–394.
    OpenUrlCrossRefPubMed
  10. ↵
    1. Crick F
    (1984) Function of the thalamic reticular complex: the searchlight hypothesis. Proc Natl Acad Sci USA 81:4586–4590.
    OpenUrlAbstract/FREE Full Text
  11. ↵
    1. Crunelli V,
    2. Leresche N
    (1991) A role for GABAB receptors in excitation and inhibition of thalamocortical cells. Trends Neurosci 14:16–21.
    OpenUrlCrossRefPubMed
  12. ↵
    1. Deschênes M,
    2. Paradis M,
    3. Roy JP,
    4. Steriade M
    (1984) Electrophysiology of neurons of lateral thalamic nuclei in cat: resting properties and burst discharges. J Neurophysiol 51:1196–1219.
    OpenUrlPubMed
  13. ↵
    1. Deschênes M,
    2. Bourassa J,
    3. Pinault D
    (1994) Corticothalamic projections from layer V cells in rat are collaterals of long-range corticofugal axons. Brain Res 664:215–219.
    OpenUrlCrossRefPubMed
  14. ↵
    1. Deschênes M,
    2. Bourassa J,
    3. Doan VD,
    4. Parent A
    (1996) A single-cell study of the axonal projections arising from the posterior intralaminar thalamic nuclei in the rat. Eur J Neurosci 8:329–343.
    OpenUrlCrossRefPubMed
  15. ↵
    1. Deschênes M,
    2. Veinante P,
    3. Zhang Z-W
    (1998) The organization of corticothalamic projections: reciprocity versus parity. Brain Res Rev 28:286–308.
    OpenUrlCrossRefPubMed
  16. ↵
    1. Desimone R,
    2. Duncan J
    (1995) Neural mechanisms of selective visual attention. Annu Rev Neurosci 18:193–222.
    OpenUrlCrossRefPubMed
  17. ↵
    1. Diamond ME,
    2. Armstrong-James M,
    3. Ebner FF
    (1992a) Somatic sensory responses in the rostral sector of the posterior group (POm) and the ventral posterior medial nucleus (VPM) of the rat thalamus. J Comp Neurol 318:462–476.
    OpenUrlCrossRefPubMed
  18. ↵
    1. Diamond ME,
    2. Armstrong-James M,
    3. Budway MJ,
    4. Ebner FF
    (1992b) Somatic sensory responses in the rostral sector of the posterior group (POm) and in the ventral posterior medial nucleus (VPM) of the rat thalamus: dependence on the barrel field cortex. J Comp Neurol 319:66–84.
    OpenUrlCrossRefPubMed
  19. ↵
    1. Dong WK,
    2. Ryu H,
    3. Wagman IH
    (1978) Nociceptive responses of neurons in medial thalamus and their relationship to spinothalamic pathways. J Neurophysiol 41:1592–1613.
    OpenUrlPubMed
  20. ↵
    1. Groenewegen HJ,
    2. Berendse HW
    (1994) The specificity of the “nonspecific” midline and intralaminar thalamic nuclei. Trends Neurosci 17:52–57.
    OpenUrlCrossRefPubMed
  21. ↵
    1. Grunwerg BS,
    2. Krauthamer GM
    (1992) Sensory responses of intralaminar thalamic neurons activated by the superior colliculus. Exp Brain Res 88:541–550.
    OpenUrlPubMed
  22. ↵
    1. Guillery RW
    (1995) Anatomical evidence concerning the role of the thalamus in corticocortical communication: a brief review. J Anat 187:583–592.
    OpenUrlPubMed
  23. ↵
    1. Guillery RW,
    2. Feig SL,
    3. Lozsádi DA
    (1998) Paying attention to the thalamic reticular nucleus. Trends Neurosci 21:28–32.
    OpenUrlCrossRefPubMed
  24. ↵
    1. Horikawa K,
    2. Armstrong WE
    (1988) A versatile means of intracellular labeling: injection of biocytin and its detection with avidin conjugates. J Neurosci Methods 25:1–11.
    OpenUrlCrossRefPubMed
  25. ↵
    1. Houser CR,
    2. Vaughn JE,
    3. Barber RP,
    4. Roberts E
    (1980) GABA neurons are the major cell type of the nucleus reticularis thalami. Brain Res 200:341–354.
    OpenUrlCrossRefPubMed
  26. ↵
    1. Jahnsen H,
    2. Llinás R
    (1984) Electrophysiological properties of guinea-pig thalamic neurones: an in vitro study. J Physiol (Lond) 349:205–226.
    OpenUrlCrossRefPubMed
  27. ↵
    1. Jones EG
    (1975) Some aspects of the organization of the thalamic reticular complex. J Comp Neurol 162:285–308.
    OpenUrlCrossRefPubMed
  28. ↵
    1. Jones EG
    (1985) The thalamus. (Plenum, New York).
  29. ↵
    1. Kha HT,
    2. Finkelstein DI,
    3. Tomas D,
    4. Drago J,
    5. Pow DV,
    6. Horne MK
    (2001) Projections from the substantia nigra pars reticulata to the motor thalamus of the rat: single axon reconstructions and immunohistochemical study. J Comp Neurol 440:20–30.
    OpenUrlCrossRefPubMed
  30. ↵
    1. Kim U,
    2. McCormick DA
    (1998) The functional influence of burst and tonic firing mode on synaptic interactions in the thalamus. J Neurosci 18:9500–9516.
    OpenUrlAbstract/FREE Full Text
  31. ↵
    1. Kolmac CI,
    2. Mitrofanis J
    (1997) Organisation of the reticular thalamic projection to the intralaminar and midline nuclei in rats. J Comp Neurol 377:165–178.
    OpenUrlCrossRefPubMed
  32. ↵
    1. LaBerge D,
    2. Carter M,
    3. Brown V
    (1992) A network simulation of thalamic circuit operations in selective attention. Neural Comp 4:318–331.
    OpenUrlCrossRef
  33. ↵
    1. Lee SM,
    2. Friedberg MH,
    3. Ebner FF
    (1994a) The role of GABA-mediated inhibition in the rat ventral posterior medial thalamus. I. Assessment of receptive field changes following thalamic reticular nucleus lesions. J Neurophysiol 71:1702–1715.
    OpenUrlPubMed
  34. ↵
    1. Lee SM,
    2. Friedberg MH,
    3. Ebner FF
    (1994b) The role of GABA-mediated inhibition in the rat ventral posterior medial thalamus. II. Differential effects of GABAA and GABAB receptor antagonists on responses of VPM neurons. J Neurophysiol 71:1716–1726.
    OpenUrlPubMed
  35. ↵
    1. Lizier C,
    2. Spreafico R,
    3. Battaglia G
    (1997) Calretinin in the thalamic reticular nucleus of the rat: distribution and relationship with ipsilateral and contralateral efferents. J Comp Neurol 377:217–233.
    OpenUrlPubMed
  36. ↵
    1. Lo F-S,
    2. Sherman SM
    (1994) Feedback inhibition in the cat's lateral geniculate nucleus. Exp Brain Res 100:365–368.
    OpenUrlPubMed
  37. ↵
    1. Macchi G,
    2. Bentivoglio M
    (1986) The thalamic intralaminar nuclei and the cerebral cortex. in Cerebral cortex, Vol 5, Sensory-motor areas and aspects of cortical connectivity, eds Jones EG, Peters A (Plenum, New York), pp 355–401.
  38. ↵
    1. MacLeod NK,
    2. James TA,
    3. Kilpatrick IC,
    4. Starr MS
    (1980) Evidence for a GABAergic nigrothalamic pathway in the rat. II. Electrophysiological studies. Exp Brain Res 40:55–61.
    OpenUrlPubMed
  39. ↵
    1. Matsumoto N,
    2. Minamimoto T,
    3. Graybiel AM,
    4. Kimura M
    (2001) Neurons in the thalamic CM-Pf complex supply striatal neurons with information about behaviourally significant sensory events. J Neurophysiol 85:960–976.
    OpenUrlPubMed
  40. ↵
    1. Minamimoto T,
    2. Kimura M
    (2002) Participation of the thalamic CM-Pf complex in attentional orienting. J Neurophysiol 87:3090–3101.
    OpenUrlPubMed
  41. ↵
    1. Miyashita E,
    2. Keller A,
    3. Asanuma H
    (1994) Input-output organization of the rat vibrissal motor cortex. Exp Brain Res 99:223–232.
    OpenUrlCrossRefPubMed
  42. ↵
    1. Oertel WH,
    2. Tappaz ML,
    3. Berod A,
    4. Mugnaini E
    (1982) Two-color immunohistochemistry for dopamine and GABA neurons in rat substantia nigra and zona incerta. Brain Res Bull 9:463–474.
    OpenUrlCrossRefPubMed
  43. ↵
    1. Peschanski M,
    2. Guilbaud G,
    3. Gautron M
    (1981) Posterior intralaminar region in rat: neuronal responses to noxious and nonnoxious cutaneous stimuli. Exp Neurol 72:226–238.
    OpenUrlCrossRefPubMed
  44. ↵
    1. Pinault D,
    2. Deschênes M
    (1998a) Anatomical evidence for a mechanism of lateral inhibition in the rat thalamus. Eur J Neurosci 10:3462–3469.
    OpenUrlCrossRefPubMed
  45. ↵
    1. Pinault D,
    2. Deschênes M
    (1998b) Projection and innervation patterns of individual thalamic reticular axons in the thalamus of the adult rat: a three-dimensional, graphic, and morphometric analysis. J Comp Neurol 391:180–203.
    OpenUrlCrossRefPubMed
  46. ↵
    1. Pinault D,
    2. Bourassa J,
    3. Deschênes M
    (1995) The axonal arborization of single thalamic reticular neurons in the somatosensory thalamus of the rat. Eur J Neurosci 7:31–40.
    OpenUrlCrossRefPubMed
  47. ↵
    1. Porter LL,
    2. White EL
    (1983) Afferent and efferent pathways of the vibrissal region of primary motor cortex in the mouse. J Comp Neurol 214:279–289.
    OpenUrlCrossRefPubMed
  48. ↵
    1. Power BD,
    2. Kolmac CI,
    3. Mitrofanis J
    (1999) Evidence for a large projection from the zona incerta to the dorsal thalamus. J Comp Neurol 404:554–565.
    OpenUrlCrossRefPubMed
  49. ↵
    1. Rose JE
    (1942) The ontogenetic development of the rabbit's diencephalon. J Comp Neurol 7:61–129.
    OpenUrl
  50. ↵
    1. Salt TE
    (1989) Gamma-aminobutyric acid and afferent inhibition in the cat and rat ventrobasal thalamus. Neuroscience 28:17–26.
    OpenUrlCrossRefPubMed
  51. ↵
    1. Sanchez-Vives MV,
    2. McCormick DA
    (1997) Functional properties of perigeniculate inhibition of dorsal lateral geniculate nucleus thalamocortical neurons in vitro. J Neurosci 17:8880–8893.
    OpenUrlAbstract/FREE Full Text
  52. ↵
    1. Scheibel AB
    (1981) The problem of selective attention: a possible structural substrate. in Brain mechanisms of perceptual awareness, eds Pompeiano O, Ajmone-Marsan C (Raven, New York), pp 319–326.
  53. ↵
    1. Scheibel ME,
    2. Scheibel AB
    (1966) The organization of the nucleus reticularis thalami: a Golgi study. Brain Res 1:43–62.
    OpenUrlCrossRefPubMed
  54. ↵
    1. Schlag J,
    2. Schlag-Rey M
    (1984) Visuomotor functions of central thalamus in monkey. II. Unit activity related to visual events, targeting, and fixation. J Neurophysiol 51:1175–1195.
    OpenUrlPubMed
  55. ↵
    1. Schlag J,
    2. Lehtinen I,
    3. Schlag-Rey M
    (1974) Neuronal activity before and during eye movements in thalamic internal medullary lamina of the cat. J Neurophysiol 37:982–995.
    OpenUrlPubMed
  56. ↵
    1. Schlag-Rey M,
    2. Schlag J
    (1977) Visual and presaccadic neuronal activity in thalamic internal medullary lamina of cat: a study of targeting. J Neurophysiol 40:156–173.
    OpenUrlPubMed
  57. ↵
    1. Schlag-Rey M,
    2. Schlag J
    (1984) Visuomotor functions of central thalamus in monkey. I. Unit activity related to spontaneous eye movements. J Neurophysiol 51:1149–1174.
    OpenUrlPubMed
  58. ↵
    1. Sherman SM
    (2001) Tonic and burst firing: dual modes of thalamocortical relay. Trends Neurosci 24:122–126.
    OpenUrlCrossRefPubMed
  59. ↵
    1. Sherman SM,
    2. Guillery RW
    (1996) Functional organization of thalamocortical relays. J Neurophysiol 76:1367–1395.
    OpenUrlPubMed
  60. ↵
    1. Sherman SM,
    2. Guillery RW
    (1998) On the actions that one nerve cell can have on another: distinguishing “drivers” from “modulators.” Proc Natl Acad Sci USA 95:7121–7126.
    OpenUrlAbstract/FREE Full Text
  61. ↵
    1. Sherman SM,
    2. Guillery RW
    (2001) Exploring the thalamus. (Academic, San Diego).
  62. ↵
    1. Sherman SM,
    2. Koch C
    (1986) The control of retinogeniculate transmission in the mammalian lateral geniculate nucleus. Exp Brain Res 63:1–20.
    OpenUrlPubMed
  63. ↵
    1. Shosaku A
    (1986) Cross-correlation analysis of a recurrent inhibitory circuit in the rat thalamus. J Neurophysiol 55:1030–1043.
    OpenUrlPubMed
  64. ↵
    1. Skinner JE,
    2. Yingling CD
    (1977) Central gating mechanisms that regulate event-related potentials and behavior. A neural model for attention. Prog Clin Neurophysiol 1:30–69.
    OpenUrl
  65. ↵
    1. Steriade M,
    2. Parent A,
    3. Hada J
    (1984) Thalamic projections of nucleus reticularis thalami of cat: a study using retrograde transport of horseradish peroxidase and fluorescent tracers. J Comp Neurol 229:531–547.
    OpenUrlCrossRefPubMed
  66. ↵
    1. Strick P
    (1976) Activity of ventrolateral thalamic neurons during arm movement. J Neurophysiol 39:1032–1044.
    OpenUrlPubMed
  67. ↵
    1. Vitek JL,
    2. Ashe J,
    3. DeLong MR,
    4. Alexander GE
    (1994) Physiologic properties and somatotopic organization of the primate motor thalamus. J Neurophysiol 71:1498–1513.
    OpenUrlPubMed
  68. ↵
    1. Vitek JL,
    2. Ashe J,
    3. DeLong MR,
    4. Kaneoke Y
    (1996) Microstimulation of primate motor thalamus: somatotopic organization and differential distribution of evoked motor responses among subnuclei. J Neurophysiol 75:2486–2495.
    OpenUrlPubMed
  69. ↵
    1. Warren RA,
    2. Jones EG
    (1994) Glutamate activation of cat thalamic reticular nucleus: effects on response properties of ventroposterior neurons. Exp Brain Res 100:215–226.
    OpenUrlPubMed
Back to top

In this issue

Email
Print
View Full Page PDF
Citation Tools
Respond to this article
New Intrathalamic Pathways Allowing Modality-Related and Cross-Modality Switching in the Dorsal Thalamus
John W. Crabtree, John T. R. Isaac
Journal of Neuroscience 1 October 2002, 22 (19) 8754-8761; DOI: 10.1523/JNEUROSCI.22-19-08754.2002
Twitter logo Facebook logo Mendeley logo

Jump to section

Keywords

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.