Article Figures & Data
Figures
- Fig. 1.
Tissue levels of 5-HT/5-HIAA in the forebrain neocortex (CTX) and hindbrain of pCPA-treated mice. pCPA treatment regimens are indicated on theabscissa. Tissues levels of 5-HT and 5-HIAA levels were determined by C18 reverse-phase HPLC and are expressed as nanomoles per gram of tissue. Data represent the means ± SEM of measurements obtained from four animals per treatment group. A Student's t test was used to test for the significance of differences between groups. *p < 0.05; **p < 0.001.
- Fig. 2.
Expression of 5-HT2C mRNA isoforms in the forebrain neocortex of drug-naive mice and mice treated with either pCPA or DOI. Comparison of the percentages of the major edited isoforms (top) and percentages of editing at the five editing sites (bottom). The data represent the means ± SEM of percentages determined for controls (n = 4; 212 sequences), pCPA-treated animals (n = 4 for each of the two treatment regimens; 218 and 240 sequences), and DOI-treated animals (n = 4; 228 sequences). Data were compared by a one-way ANOVA, followed by Tukey–Kramer multiple comparisons test. Top, *p < 0.05; **p < 0.01. Bottom, *p < 0.04; **p < 0.02. Partially edited transcripts involving the C′ site are mRNA isoforms resulting from the editing combinations ABC′D, AC′D, C′D, and C′. Transcripts edited at both C′ and C sites included mRNA isoforms resulting from the editing combinations ABC′CD, AC′CD, AC′C, ABC′C, C′CD, and C′C. The latter two were found only in DOI-treated animals.
- Fig. 3.
Expression levels of 5-HT2C mRNA in the forebrain neocortex of drug-naive mice and pCPA- and DOI-treated mice. Southern blot of exponential RT-PCR amplification of cDNA encoding a 270-nucleotide-long sequence of 5-HT2CmRNA sequence containing the edited region. Aliquots of the PCR reactions were removed after 10, 15, 20, and 25 cycles of amplification. For each treatment group, representative results obtained from two mice are shown, and, for comparison, PCR amplification of 0.01 ng of plasmid DNA encoding the full-length 5-HT2C receptor is shown on top. Blots were exposed to film for 3 hr. CMV, Cytomegalovirus;pRC, plasmid vector RC (Invitrogen).
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