Abstract
Norepinephrine (NE), glutamate (Glu), and GABA have been identified as important neurotransmitters governing neuroendocrine mechanisms represented in the paraventricular nucleus of the hypothalamus (PVH). Microinjection studies were used to compare the efficacy of these transmitter mechanisms in stimulating PVH output neurons. Local administration of NE provoked an increase in plasma corticosterone levels and Fos induction in the both the parvocellular and magnocellular divisions of the nucleus. This treatment also stimulated a robust increase in corticotropin-releasing factor (CRF) heteronuclear (hn) RNA in the parvocellular PVH and a more subtle, although reliable, increase in arginine vasopressin (AVP) hnRNA in this same compartment. Local administration of the GABAAreceptor antagonist bicuculline methiodide (BMI) resulted in increased plasma corticosterone and, in contrast to NE treatment, Fos induction limited primarily to the parvocellular PVH. BMI elicited marked increases in both CRH and AVP hnRNAs within the parvocellular division of the nucleus. Over a wide range of concentrations, Glu failed to produce reliable increases in corticosterone secretion and induced only weak activational responses limited primarily to non-neurosecretory regions of the PVH. Local Glu administration did, however, provoke Fos induction in identified GABAergic neurons immediately adjoining the PVH, suggesting that the muted response to Glu may be a consequence of concurrent activation of local inhibitory interneurons. These results support a differential involvement of adrenergic, glutamatergic and GABAergic mechanisms in regulating neurosecretory populations of the PVH and suggest that involvement of local circuit neurons must be carefully considered in the interpretation of microinjection studies.
The paraventricular hypothalamic nucleus (PVH) is a key site for marshaling integrated and adaptive responses to stress. The PVH houses (1) parvocellular neurosecretory neurons expressing corticotropin-releasing factor (CRF) for the initiation of the endocrine (pituitary–adrenal) arm of the generalized stress response, (2) neurons projecting to central autonomic cell groups, including sympathetic preganglionics that constitute the neural arm of the stress response, and (3) magnocellular neurosecretory cells that release oxytocin and arginine vasopressin (AVP) from the posterior pituitary in response to more specific physiological demands (Swanson and Sawchenko, 1983). The advent of immediate-early gene-based functional mapping (Morgan and Curran, 1991) has made it clear that these populations can be recruited differentially, with the parvocellular CRF population being activated nearly invariantly in response to acute stress (Ceccatelli et al., 1989; Chan et al., 1993).
Such approaches have also facilitated efforts to define extended circuitries underlying the recruitment of PVH effector neurons to particular insults. Although candidate afferent mediators of PVH responses have been identified in some stress models, less attention has been directed toward identifying neurotransmitter systems involved in stressor-specific regulation of PVH compartments. The principal ones implicated in PVH control include norepinephrine (NE), GABA, and glutamate (Glu). The PVH receives a dense catecholaminergic innervation from the caudal medulla, which serves mainly to relay visceral sensory information (Sawchenko and Swanson, 1981; Cunningham and Sawchenko, 1988). Accordingly, these pathways have been implicated in mediating the effects of systemic stressors, such as hemorrhage and immune challenges (Plotsky et al., 1989; Ericsson et al., 1994; Chan et al., 2001) (see also Plotsky, 1987). GABA is the principal inhibitory neurotransmitter in the PVH (Decavel and van den Pol, 1990). GABAergic inputs originate mainly from local hypothalamic sources and are thought to impart limbic and cortical influences on PVH mechanisms (Roland and Sawchenko, 1993; Boudaba et al., 1996; Herman and Cullinan, 1997). Finally, glutamate has been suggested as the dominant excitatory neurotransmitter in neuroendocrine regulation (van den Pol et al., 1990; van den Pol and Trombley, 1993; Brann, 1995; Oliver et al., 1996), although neither the origins of glutamatergic inputs nor their role in initiating hypothalamo–pituitary–adrenal (HPA) responses have been defined clearly (Costa et al., 1992; Patchev et al., 1994; Oliver et al., 1996). One goal of the present study was to compare the effects of manipulating these transmitter systems on PVH regulatory mechanisms, focusing on substrates for HPA control.
Establishing functional linkages between circuitry, transmitters, and target neuron effects is complicated by the fact that PVH cell types may use multiple effector molecules. Thus, although CRF provides a neurochemical signature for HPA-related hypophysiotropic neurons, it does not act alone in this regard. These neurons can synthesize additional peptides, notably AVP, which synergize with CRF to determine pituitary–adrenal output (Gillies et al., 1982; Rivier and Vale, 1983;Hauger and Aguilera, 1993). AVP is regulated more dynamically than CRF in this context and is generally viewed as the major determinant of situation-specific drive on the axis (Antoni, 1993; Kovács et al., 2000). Recent analyses using probes to intronic sequences to monitor transcriptional activation directly (Herman et al., 1991, 1992) describe distinct time courses of stress-induced activation of the expression of these genes, with elevations in primary CRF and AVP transcripts occurring at 5–30 and 60–120 min, respectively (Imaki et al., 1995; Kovács and Sawchenko, 1996; Ma et al., 1997). This provides a framework for examining how regulatory influences participate in this differential control, and selective activation of CRF transcription in response to local NE injection has been reported (Itoi et al., 1999). A second objective of the present study was to confirm and extend this analysis.
Parts of this work have been published previously in abstract form (Cole and Sawchenko, 1998).
MATERIALS AND METHODS
Animals and treatment paradigms. Adult male Sprague Dawley albino rats (250–300 gm) were used in all experiments. Animals were housed in a colony room under controlled temperature and humidity, with food and water available ad libitum. Lights were on between 7:00 A.M. and 7:00 P.M. The rats were adapted to handling for at least 3 d before any treatment. In all experiments, group sizes were n = 4–8. All protocols were approved by the Institutional Animal Care and Use Committee of the Salk Institute.
Intracerebral microinjection. Rats were anesthetized and implanted with unilateral 24 ga guide cannulas aimed to terminate 1 mm dorsal to the PVH. Coordinates used for PVH microinjections were 1.8 mm posterior to bregma, 0.5 mm lateral to the midline, and 5.9 mm ventral from the dura. After 7 d recovery, during which time animals were handled and subjected to daily mock injections, rats were challenged with drug or vehicle injections via 30 ga injectors that extended 1.0 mm beyond the tip of the guide. All drugs were administered in 200 nl of sterile pyrogen-free saline. Drug doses were based on the range of concentrations known to elicit relevant physiological responses in previous microinjection studies (Leibowitz, 1978; Lipski et al., 1988; Itoi et al., 1994; Huhman et al., 1995; Soltis et al., 1997; Chan et al., 1998). Groups of animals received 1 nmol of l-glutamate (Sigma, St. Louis, MO), 100 pmol of bicuculline methiodide (BMI) (Fluka, Buchs, Switzerland), 25 nmol of norepinephrine bitartrate (Sigma), or saline vehicle. After injections, animals were returned to their home cages, in which they remained undisturbed until being killed 20 or 90 min later. These time points were selected to approximate those at which peak stress-induced elevations of CRF heteronuclear (hn) RNA (20 min) or both AVP hnRNA and Fos-immunoreactivity (IR) (90 min) have been described within the parvocellular division of the PVH.
To determine the approximate extent of diffusion after microinjection, 200 nl of [3H]NE (DuPont NEN, Boston, MA) was injected into the PVH of a separate group of animals. [3H]NE (0.1 μCi) was added to cold NE to make up a 50 nmol/0.1 μl solution.
Blood sampling and plasma corticosterone assay. Rats bearing cerebral guide cannulas were implanted with indwelling jugular venous catheters 2 d before experimentation. Catheters were made using polyethylene-50 tubing with SILASTIC (Dow Corning, Corning, NY) tips that terminated near the atrium and were exteriorized in an interscapular position. Testing was performed in freely moving rats in their home cages starting at 9:00 A.M., before the circadian increase in plasma corticosterone levels. Repeated blood samples (100 μl) were taken before and at 5, 15, 30, 60, and 90 min after central injections of drug or vehicle administered as described above. Samples were collected in chilled tubes containing 2 μl of 20% EDTA and centrifuged, and plasma was stored at −20°C until assay. Plasma corticosterone was measured by radioimmunoassay (ImmuChem; ICN Biomedicals, Cleveland, OH), using a rabbit antiserum raised against corticosterone-3-carboxymethyloxime:BSA. The sensitivity of the assay is 5 ng/ml; intra-assay and interassay coefficients of variation are <10.3 and < 7.2%, respectively.
Perfusion and tissue processing. Animals were perfused under chloral hydrate anesthesia (350 mg/kg, i.p.) via the ascending aorta with saline, followed by 500 ml of ice-cold 4% paraformaldehyde in 0.1m borate buffer at pH 9.5, delivered over 20 min. Brains were post-fixed in the perfusate for 2 hr and then stored overnight in potassium PBS with 10% sucrose at 4°C. Multiple series of 30-μm-thick frozen sections, collected at 150 μm intervals, were cut on a sliding microtome and collected into cryoprotectant, in which they were stored at −20°C until use.
Immunohistochemistry. Fos-IR was localized using a polyclonal antiserum raised in rabbit against synthetic human Fos (amino acid positions 3–15), at a dilution of 1:5000 (Santa Cruz Biotechnology, Santa Cruz, CA). Immunohistochemistry was performed using a conventional avidin–biotin immunoperoxidase protocol (Sawchenko et al., 1990) and Vectastain Elite reagents (Vector Laboratories, Burlingame, CA). Tissue was pretreated with hydrogen peroxide (0.3%) before addition of the primary antibody to squelch endogenous peroxidase activity in the tissue. The diaminobenzidine reaction was amplified using nickel ammonium sulfate.
To compare the distribution and relative strength of activational responses to drug treatments, counts of the number of Fos-IR cells in cytoarchitectonically defined subregions of the PVH (Swanson and Kuypers, 1980) were generated in complete series of sections through the caudal PVH and corrected for double-counting errors (Abercrombie, 1946).
Hybridization histochemistry. Hybridization histochemistry was performed using [35S]UTP (DuPont NEN) labeled antisense cRNA probes. The AVP hnRNA probe was transcribed from a 700 bp PVUII fragment of intron I of the rat vasopressin gene subcloned into pGEM3 (Herman et al., 1991). The CRF hnRNA probe was transcribed from a 500 bp PVUII fragment of the single intron in the CRF gene from a rat genomic CRF clone, subcloned into pBluescript (Dr. A. Ericsson, The Salk Institute). The identity and specificity of each probe has been established by partial sequence and/or Northern blot analysis, and each yields hybridization patterns in accord with available evidence. Sense strand probes labeled to similar specific activities produced no clear positive hybridization signals in the PVH or elsewhere in brain (data not shown).
Hybridization and autoradiographic techniques were modified as described previously (Simmons et al., 1989). Tissue sections were mounted on poly-l-lysine-coated slides, post-fixed with formalin for 30 min, digested with 10 μg/ml proteinase K, acetylated with 0.25% acetic anhydride, and dehydrated. Probes were labeled to specific activities on the order of 1–3 × 109 dpm/μg and applied to the slide at concentrations of ∼107 cpm/ml, overnight at 56–58°C in a solution containing 50% formamide, 0.3m NaCl, 10 mm Tris, pH 8.0, 1 mmEDTA, 0.05% tRNA, 10 mm dithiothreitol, 1× Denhardt's solution, and 10% dextran sulfate, after which they were treated with 20 μg/ml ribonuclease A for 30 min at 37°C and washed in 15 mm NaCl/1.5 mm sodium citrate at 65–85°C. Slides were then dehydrated and exposed to x-ray films (β-max; Eastman Kodak, Rochester, NY) for 1–2 d. They were then coated with Kodak NTB-2 liquid emulsion, and exposed at 4°C for a length of time determined by the strength of signal on film. CRH hnRNA slides were exposed for 45 d. AVP hnRNA slides were exposed for 21 d to optimize the localization of AVP hnRNA in the parvocellular division of the PVH. Because of the long exposure time, AVP hnRNA in the magnocellular division of the PVH is beyond the linear range of the assay and could not be quantified in the material used in these experiments. Slides were developed with Kodak D-19 and fixed with Kodak rapid fixer.
Combined immunohistochemistry and hybridization histochemistry. The protocol for combining immunoperoxidase labeling with isotopic in situ hybridization involves minor modifications (Chan et al., 1993) of the procedure described by Watts and Swanson (1989). Immunolocalization of Fos-IR was combined with hybridization histochemical detection of GAD-67 mRNA. GAD-67 mRNA was localized using a 35S-labeled cRNA probe (Dr. A. J. Tobin, University of California at Los Angeles, Los Angeles, CA). Immunostaining was performed first, and the individual methods are modified as follows: (1) nonimmune (blocking) sera, potential sources of RNase contamination, were replaced with 2% heparin sulfate and 2% BSA; (2) nickel enhancement steps were eliminated from the immunostaining protocol because nickel-based reaction product does not survive the hybridization steps; (3) tissue pretreatment with hydrogen peroxide was omitted; and (4) Nissl counterstaining was omitted.
Comparisons of the ability of Glu injections to activate local GABAergic neurons were performed by counting the number of Fos-immunoreactive cells in sections through the central part of the anterior hypothalamic area of Glu- and saline-treated rats and assessing the proportion of these that were overlain by clusters of reduced silver grains whose density exceeded five times background levels, as determined by grain density counts over cell-sized areas in the thalamus. Average counts per section were generated in three sections through representative levels of the anterior hypothalamic area in each animal and then averaged again to obtain group means.
Data collection and analysis. Material to be compared was hybridized using a common batch of probe. Semiquantitative densitometric analysis of relative levels of RNAs was performed on emulsion-coated slides. Slides were coded for analysis to obscure treatment status. Densitometric analysis of autoradiographic images was performed using Macintosh-driven NIH Image software (version 1.62). The medial parvocellular subdivision of the PVH (Swanson and Kuypers, 1980) was defined from Nissl staining patterns and aligned with corresponding dark-field images of hybridized sections by redirected sampling. Analysis of AVP hnRNA is confounded by the presence of densely labeled magnocellular neurons that are scattered within the parvocellular division of the PVH. Because this can mask or dilute changes assessed using densitometric analysis, a second approach to quantification was used. This involved simply counting the number of nuclei in the medial parvocellular division of the PVH, which contained clusters of silver grains whose density was more than five times background. Abercrombie's method (Abercrombie, 1946) was used to correct for double-counting errors. Data from all measures are expressed as mean ± SEM and were analyzed by one-way ANOVA.
RESULTS
Cannula placements
To determine the approximate extent of diffusion after microinjection, 200 nl of [3H]NE was injected into the PVH in four rats (Fig.1A,B). [3H]NE was well confined to the ipsilateral side and was not detectable in ventricular spaces. The radius of diffusion was <1.0 mm from the tip of the injector. As a consequence, data from experimental animals whose cannula tips lay beyond 1 mm of the PVH, or invaded either the PVH itself or the third ventricle, were not included in the analysis. Using the nomenclature ofSwanson and Kuypers (1980), placements varied from the level of the anterior parvocellular part of the PVH (referred to here as rostral injections) to that of the maximum development of the posterior magnocellular part of the nucleus (mid-PVH injections). Representative injection sites are shown in Figure 1C.
The extent and placement of microinjections into the PVH. A, Dark-field photomicrograph showing the location and extent of a microinjection of 200 nl of [3H]NE (asterisk) into the region just dorsal to the PVH (arrow). B, Enlarged view of injection site showing that the gradient of diffused injectate spreads to involve the PVH. C, Bright-field photomicrographs of immunoperoxidase preparations (for Fos-IR) to show representative microinjection cannula placements in rats injected with saline (C), glutamate (C′) or norepinephrine (C"). Note that the placements shown inC and C" are centered at the level of the mid-PVH, although the one illustrated in C′ is situated more rostrally. Rats whose cannula tips were found to lay within a ventricle or >1 mm from the dorsal and/or lateral margin of the PVH were excluded from analysis. Induced Fos-IR is observed as a black reaction product. Magnifications: A, 8.5×;B, 70×; C, 25×. cc, Corpus callosum; Ctx, cerebral cortex;fx, fornix; HF, hippocampal formation;ic, internal capsule; och, optic chiasm;ot, optic tract; SCh, suprachiasmatic nucleus.
Plasma corticosterone
To examine the effects of neurotransmitter microinjection on secretory activity of the HPA axis, plasma corticosterone levels were monitored in a separate group of rats. In response to microinjection of saline, hormone titers displayed a relatively mild, but significant (p < 0.05), increase from resting levels of 37 ± 26 ng/ml in rats to 170 ± 77 ng/ml at 30 min after injection (Fig. 2), presumably attributable to the effects of handling and/or repeated blood sampling. Unilateral injection of NE (25 nmol) or the GABAA receptor antagonist BMI (100 pmol) provoked corticosterone secretory responses that were comparable (387 ± 171 and 362 ± 42 ng/ml, respectively) and significantly greater than those seen in response to saline at the 30 min peak time point (p < 0.05). In contrast, Glu (1 nmol) did not produce a significant elevation in plasma corticosterone above control levels at 30 min (236 ± 86 ng/ml) or any other postinjection time point (p > 0.05).
Plasma corticosterone levels are significantly increased above control levels by microinjections of norepinephrine or bicuculline, but not glutamate, into the PVH. A, Mean ± SEM plasma corticosterone (CORT) concentrations at various time points after PVH microinjection of saline (Sal), 25 nmol of NE, 100 pmol of BMI, or 1 nmol of Glu. n = 4 per group.B, Compared with saline-injected controls, a significant elevation in peak plasma corticosterone is observed at 30 min after NE or BMI injection (p < 0.05 by one-way ANOVA). Plasma corticosterone was not significantly increased after Glu microinjection.
Fos induction
Animals killed 2 hr after saline microinjection displayed little Fos-IR within the PVH, proper (Fig. 3, Table 1). The scattered Fos-immunoreactive neurons seen in immediately adjoining regions were similar in density and distribution to that described previously in nonmanipulated rats (Chan et al., 1993). Microinjection of 25 nmol of NE provoked a robust induction of Fos-IR that was strongly focused in the PVH and manifest across all its functional compartments (Fig. 3, Table 1). This occurred in the absence of any overt behavioral response to drug treatment. Responsive subregions included aspects of the medial parvocellular part of the nucleus in which hypophysiotropic CRF-expressing neurons are concentrated, components of the magnocellular division that harbor both oxytocin- and vasopressin-producing cells, and each of the subdivisions (ventral medial, dorsal, and lateral parvocellular) that give rise to autonomic-related projections to the brainstem and spinal cord. The intensity of Fos-immunoreactive labeling tended consistently to be greater in magnocellular than parvocellular regions. When injection sites were localized at the level of the mid-PVH, this pervasive pattern of PVH activation displayed a strong ipsilateral predominance, with no responsiveness noted in the supraoptic nucleus, another major seat of magnocellular neurosecretory neurons. In contrast, animals that received more rostrally placed NE injections manifest a similar pattern of Fos induction in the PVH, but that was bilaterally symmetrical and prominently included the supraoptic nucleus, again on both sides of the brain (Fig. 4). Bilateral activation of the PVH after rostrally placed microinjections is unlikely to be attributable to the diffusion of drug based on results obtained with [3H]NE (Fig. 1). Alternatively, it is possible that the bilateral activation may be a secondary response to some physiological consequence of NE microinjection. Our data argue against this, because brainstem cell groups such as the nucleus of the solitary tract and/or ventrolateral medulla that would likely mediate such an effect were not observed to be responsive in these animals (data not shown). Finally, the bilateral hypothalamic response pattern might result from the involvement of a cell group situated in proximity to rostrally placed injections that is known to both receive a noradrenergic input and to project bilaterally to the PVH and supraoptic nucleus. The median preoptic nucleus meets these criteria and its involvement provides a viable explanation for the bilateral activation pattern, because rats that received rostrally placed PVH injections consistently exhibited Fos induction in this cell group, whereas those whose cannulas were centered at the level of the mid-PVH did not (Fig. 4).
Distinct patterns of cellular activation evoked by microinjection of bicuculline or norepinephrine. Bright-field photomicrographs of sections through the PVH on the sides ipsilateral (injected; left) and contralateral (noninjected;right) to microinjections of saline (top), 25 nmol of NE (middle), or 100 pmol of BMI (bottom) at the level of the mid-PVH. Saline-treated animals displayed very low-level Fos expression whose pattern and strength was indistinguishable from that seen in nonmanipulated rats. NE provoked a robust and lateralized Fos induction, highly focussed in the PVH, that encompassed all major functional compartments of the magnocellular and parvocellular divisions of the nucleus. BMI, in contrast, gave rise to Fos induction with the PVH that was highly confined to the parvocellular division of the nucleus and equally prominent in immediately adjoining areas, outside the PVH, proper. Magnification: 60×. mp, Medial parvocellular part (PVH); pm, posterior magnocellular part (PVH); fx, fornix. Asterisk indicates injection site.
Drug-induced Fos induction in select PVH compartments 1-a
Rostrally placed norepinephrine injections give rise to bilateral activation of both the PVH and the supraoptic nucleus. Bright-field photomicrographs of sections through the PVH (top) and the supraoptic nucleus (middle) on the sides ipsilateral (injected; left) and contralateral (noninjected; right) to a microinjection of 25 nmol of NE centered at the level of the rostral PVH. In contrast to the highly lateralized effects seen after mid-PVH injections (see Fig. 3), more rostrally placed injections gave rise to a bilateral pattern of activation in the PVH, which included the supraoptic nucleus, also bilaterally. This distinctive pattern of activation could be attributable to concurrent involvement of a cell group that lay within the sphere of the injection site, exhibits NE sensitivity, and projects bilaterally to both the PVH and supraoptic nucleus. These attributes are displayed by the median preoptic nucleus (bottom), which exhibited robust Fos induction in response to NE administration at rostral, but not mid-PVH, levels. Magnification: 60×. ac, Anterior commissure;MePO, median preoptic nucleus; SO, supraoptic nucleus.
Microinjection of the GABAA receptor antagonist BMI typically provoked intense behavioral activation (increased locomotion, jumping, running, and grooming). In contrast to the focal pattern of cellular activation seen in response to NE treatment, microinjections of BMI produced a massive Fos induction centered about the tip of the injection cannula that ostensibly did not respect nuclear boundaries. That is, comparably robust responses were seen in nearly all proximal cell groups of the ventral thalamus and dorsomedial hypothalamus, including the parvocellular division of the PVH (Fig. 3, Table 1). In contrast, however, relatively few weakly labeled Fos-positive neurons were observed in the magnocellular division of the nucleus in most, but not all, animals. This basic pattern was highly lateralized and obtained regardless of the rostrocaudal level of the cannula placement. This result suggests that, in the nonstressed animal, parvocellular and magnocellular neurosecretory neurons rest under differential GABAergic control.
In line with the results of corticosterone assays, microinjection of Glu (1 nmol) provoked only a weak induction of Fos-IR in the PVH. Typically, these injections gave rise to a relatively small number of weakly labeled nuclei concentrated mainly in autonomic-related aspects of the PVH, including the dorsal, ventral medial, and lateral parts of the parvocellular division of the nucleus on the side ipsilateral to the injection (Fig. 5, Table 1). In these preparations, we observed only a modest activational response in the CRF-rich hypophysiotropic zone of the PVH and little or no apparent Fos induction in the magnocellular division of the nucleus. Comparably muted responses were seen in the PVH after doses of Glu ranging from 0.1 to 100 nmol (data not shown). In control experiments, injections of 1 nmol of Glu into the hippocampal formation gave rise to robust activational responses (Fig. 5).
Local glutamate injection produces a modest activational response in the PVH. Top, Bright-field photomicrographs showing Fos-IR expression in and around the PVH on the side ipsilateral (injected; left) and contralateral (noninjected; right) to a microinjection of 1 nmol of Glu. Only a modest activational response, localized principally to the autonomic-related dorsal and ventral medial parts of the parvocellular division of the nucleus, was observed. Note also the substantial induction of Fos-IR in regions immediately adjoining the PVH.Bottom, In contrast to the PVH, microinjection of 1 nmol of Glu into the hippocampal formation produces a marked increase in Fos-IR on the injected side of the brain, particularly in the granule cell layer of the dentate gyrus. Asterisk marks tip of injection cannula. Magnification: 60×. mp, Medial parvocellular part (PVH); pm, posterior magnocellular part (PVH); fx, fornix.
One possible explanation for the surprisingly muted response to glutamate microinjection could involve concurrent activation of GABA-mediated local inhibitory mechanisms known to reside in the perinuclear zone immediately surrounding the PVH (Roland and Sawchenko, 1993; Boudaba et al., 1996). To test the viability of this hypothesis, immunolabeling for Glu-induced Fos-IR was combined with hybridization histochemical detection of GAD-67 mRNA, a marker for GABAergic neurons. In material from control (saline-injected) animals, a substantial proportion of the relatively small number of scattered neurons in regions immediately adjoining the PVH that exhibited Fos-IR under nonstimulated conditions displayed positive hybridization signals for GAD-67 mRNA (Fig. 6). Regions displaying such double-labeled cells included the anterior and perifornical hypothalamic areas, the posteromedial part of the bed nucleus of the stria terminalis, the rostromedial zona incerta, and the areas immediately laterally and ventrally subjacent to the PVH. Animals receiving appropriately placed injections of Glu displayed an apparent increase in the number of Fos-immunoreactive neurons in the aforementioned areas that lay within the presumed effective injection site, with clear elevations evident in the anterior hypothalamic area and the perinuclear region of the PVH. Once again, the great majority of Fos-immunoreactive neurons in material from Glu-injected rats displayed positive hybridization signals for GAD-67 message (Fig. 6). Counts of the average number of double-labeled cells in one representative region, the central part of the anterior hypothalamic area, revealed a mean of 36.0 ± 6.4 cells per section in Glu-injected animals (n = 8) compared with 13.4 ± 1.1 cells per section in saline-treated controls (n = 4); these represented 74 and 79%, respectively, of all Fos-positive cells counted in this cell group under these conditions. Overall, these data indicate that at least some local GABAergic interneurons exhibit Glu sensitivity, although it remains to be determined to what extent these may contribute afferent projections to the PVH.
Glutamate microinjection activates perinuclear GABAergic neurons. Bright-field photomicrographs showing Fos-IR expression (brown nuclear product) combined with hybridization histochemical localization of GAD-67 mRNA (black cytoplasmic grains) in the anterior hypothalamic area (AHA; top) and perinuclear zone of the PVH (peri-PVH;bottom) of control (saline-injected;left) and glutamate-injected (right) rats. Under control conditions, a majority of the relatively small number of cells displaying Fos-IR are colabeled for GAD-67 mRNA. Local glutamate administration substantially increases the number of Fos-immunoreactive neurons in these regions (but not in the PVH itself); most of the glutamate-sensitive perinuclear neurons also display the marker for the GABAergic phenotype. Arrowsdenote examples of double-labeled cells. Magnification: 230×.
Transcriptional activation of the neuropeptide genes in the parvocellular division of the PVH
Previous work (Kovács and Sawchenko, 1996) has described temporally distinct patterns of stress-induced transcriptional activation of the genes encoding the two principal corticotropin secretagogues expressed by parvocellular neurosecretory neurons, with significant elevations of CRF hnRNA seen at early (5–30 min) post-stress time points and AVP intronic responses substantially later (60–120 min). To determine the extent to which NE and GABA mechanisms may mimic, and potentially participate in, this differential control, material from rats killed 20 or 90 min after microinjection of NE or BMI was analyzed for relative levels of primary CRF and AVP transcripts.
Animals receiving microinjection of saline displayed very low levels of CRF hnRNA expression in the parvocellular division of the PVH, whose relative strength and distribution was similar to that described in untreated rats. Relative to these control animals, rats killed at 20 min after either NE or BMI microinjection displayed comparable (30.6-fold and 27.5-fold), and statistically reliable (p values < 0.05), increases in relative levels of CRF hnRNA in the parvocellular division of the PVH (Fig.7). Thus, in vivo, both adrenergic receptor activation and GABAA receptor blockade can stimulate CRF gene expression.
Both NE and BMI microinjection induce CRF gene transcription in the parvocellular division of the PVH.Left, Dark-field photomicrographs depicting CRF hnRNA expression in the parvocellular division of the PVH after microinjection of saline (Sal), 100 pmol of BMI, and 25 nmol of NE. Animals were killed 20 min after microinjection. Both NE and BMI microinjection produce a robust increase in hnRNA encoding for CRH peptide in the parvocellular division of the PVH. Magnification: 45×. Right, Mean ± SEM relative levels of CRF hnRNA in the parvocellular PVH of rats killed 20 min after saline, NE, or BMI injection. n = 4–6 per group. *p < 0.05 by one-way ANOVA.
In contrast to the low basal level of CRF hnRNA expression, primary AVP transcripts were readily detectable in saline-injected control rats killed at 90 min after injection, concentrated principally in the magnocellular division of the nucleus, with a relatively small number of cells of similar size and labeling intensity scattered throughout the parvocellular region (Fig. 8). No clear evidence suggestive of AVP hnRNA expression by smaller neurons in the hypophysiotropic zone of the medial parvocellular part of the nucleus was evident under this control condition. In contrast, rats killed at the same time point after BMI, but not NE, microinjection displayed a reliable 2.7-fold increase in relative levels of AVP hnRNA in the parvocellular division of the PVH, as assessed by population densitometry (p < 0.05) (Fig. 8).
AVP gene transcription in the parvocellular division of the PVH in response to microinjection of BMI or NE.Left, Dark-field photomicrographs showing AVP hnRNA expression in the parvocellular division of the PVH after microinjection of saline (Sal), 100 pmol of BMI, and 25 nmol of NE. Animals were killed 90 min after microinjection. Whereas robust expression of the primary AVP transcripts in the magnocellular division of the PVH is evident under each condition, AVP hnRNA expression in the parvocellular PVH is induced in response to BMI injection, with a substantially more muted response seen after NE administration. Magnification: 45×. Right, Mean ± SEM relative levels of AVP hnRNA after PVH microinjection of saline, NE, and BMI, as assessed by population densitometry (open bars) and cell counts (filled bars).n = 4–6 per group. *p < 0.05 by one-way ANOVA. Only the BMI effect on AVP hnRNA was significant based on densitometric analysis, although both drugs elicited reliable increases in the number of neurons exhibiting positive hybridization signal in the parvocellular division of the PVH.
Although NE treatment failed to provoke a significant increase in AVP intronic expression, the appearance of the material suggested that small cells within the parvocellular division were manifesting induced AVP expression. Because the presence of scattered magnocellular neurons that express AVP at high basal levels is known to be capable of interfering with detection of relatively subtle changes in AVP expression in the parvocellular division of the PVH, cell counts were undertaken to assay for the presence of a reliable AVP response. This analysis revealed a significant increase in the number of neurons in the parvocellular region that manifested AVP hnRNA expression in response to NE microinjection (p < 0.05). The magnitude of this increment (42%) was somewhat less than that seen in response to BMI injection (79%) (Fig. 8), although the values did not differ significantly from one another. Thus, our findings support the view that both NE and BMI have the capacity to increase AVP hnRNA in the parvocellular division of the PVH, with the GABAA receptor antagonist manifesting greater efficacy under the conditions in force in these experiments.
DISCUSSION
Microinjection studies examined the effects of manipulating three major neurotransmitter systems on various indices of PVH effector neuron activity, focusing on parvocellular neurosecretory neurons that comprise the central limb of the HPA axis. Corticosterone secretion, Fos induction, and neuropeptide gene transcription were all activated by local adrenergic receptor stimulation or GABAAreceptor blockade but not by glutamate. Response profiles elicited by NE and BMI differed with respect to the complement of PVH effector populations that were recruited and the extent to which AVP transcriptional activation in hypophysiotropic neurons was provoked. The results support differential involvement of adrenergic, GABAergic, and glutamatergic mechanisms in governing PVH outputs. However, ancillary observations suggest that involvement of local circuit neurons may complicate the interpretation of such microinjection studies.
Norepinephrine microinjection produces robust PVH activation
Unilateral norepinephrine microinjection produced a significant increase in plasma corticosterone levels, in accord with many previous studies documenting elevations in plasma ACTH and corticosterone titers after central NE administration (Szafarczyk et al., 1987; Leibowitz et al., 1989). These effects are likely mediated principally by α1 adrenergic receptors located on CRF-expressing PVH neurons (Plotsky et al., 1987). In line with this, NE injection provoked Fos induction in the hypophysiotropic zone of the PVH and, in addition, robust activation of magnocellular neurosecretory and autonomic-related populations within the PVH. This is consistent with the broad distribution of adrenoceptor binding and expression data, with some subtypes appearing to be localized preferentially to the magnocellular (α2A: Nicholas et al., 1993; α2C: Rosin et al., 1996) or parvocellular divisions of the nucleus (α1β: Day et al., 1999) and others more broadly (α1D: Sands and Morilak, 1999). It is of interest to note that a comparably broad pattern of cellular activation is seen in the PVH in response to hypotensive hemorrhage (Badoer et al., 1992;Chan et al., 1993), and recent data support a primary involvement of ascending catecholaminergic projections in mediating PVH responses to this challenge (Chan et al., 2001).
This broad pattern of NE-induced cellular activation was primarily confined to the ipsilateral (injected) side when NE was administered at the level of the mid-PVH but was bilaterally symmetrical, with strong involvement of the supraoptic nucleus, when injections were placed more rostrally. The bilateral response may result from activation of more rostrally situated structures, such as the median preoptic nucleus, which is known to receive noradrenergic input (Saper and Levisohn, 1983) and to project bilaterally to the PVH and supraoptic nucleus (Sawchenko and Swanson, 1983).
In support of previous observations (Itoi et al., 1999), our experiments also demonstrate that NE gives rise to a rapid and reliable increase in CRF hnRNA in the parvocellular division of the PVH. In contrast to the results of Itoi et al. (1999), however, our findings also support a responsiveness of parvocellular AVP gene expression to this manipulation. This increase was not readily detectable by densitometric analysis but was revealed by cell counts. One possible explanation for this discrepancy is the exposure time used for autoradiograms. We used a longer (3 week) interval to optimize detection of relatively low level AVP hnRNA expression in the parvocellular division of the PVH, whereas Itoi et al. (1999) used a shorter exposure time (1 week) to enable concurrent analysis of the substantially higher level of expression in magnocellular neurosecretory neurons. It is possible that the shorter exposure time was not adequate to fully reveal the expression of AVP hnRNA in the parvocellular division of the PVH. In any event, our results support a capacity of local NE administration to initiate AVP transcription in parvocellular neurons, although the response was observed to be relatively weak under the experimental conditions we used.
Effects of GABA antagonism on PVH activity
Many previous studies have shown potent activational effects of BMI injections into the PVH. These include robust increases in autonomic parameters, such as blood pressure and heart rate (Martin et al., 1991; Martin and Haywood, 1993). The cellular populations in the PVH that are responsive to BMI microinjection have not yet been defined. Here we show that, despite their similar effects on plasma corticosterone levels, BMI and NE produced distinctive patterns of cellular activation within the PVH. BMI strongly and preferentially activated neurons in the parvocellular neurosecretory and autonomic subregions of the nucleus, although its effects were muted or lacking in the magnocellular compartment. This result is consistent with a study supporting predominantly parvocellular targets of perinuclear GABAergic neurons (Roland and Sawchenko, 1993) and suggests that parvocellular and magnocellular neurosecretory neurons are under differential GABAergic control. Interestingly, electrophysiological evidence indicates that magnocellular and parvocellular elements of the PVH are under similar inhibitory, GABAA receptor-mediated control by local interneurons (Tasker and Dudek, 1993). The basis for the apparent lack of congruence of the electrophysiological and anatomical data are unclear.
It should be noted that, although NE-induced activational responses were preferentially localized to the PVH, those provoked by BMI did not respect nuclear borders (Fig. 3) and may well have included local GABAergic interneurons (see below), but this was not examined directly.
BMI produced robust increases in both CRF and AVP hnRNA expression within the parvocellular PVH. This supports an involvement of GABAergic mechanisms in regulating the expression of both major corticotropin secretagogues in hypophysiotropic neurons. The fact that BMI elicited more robust AVP transcriptional activation than NE, while similarly affecting corticosterone secretion and CRF hnRNA expression, may be indicative of a preferential involvement of GABAergic mechanisms in regulating AVP dynamics in this cell type. It has been noted that perinuclear GABAergic neurons that provide input to the PVH are in position to mediate well documented, and generally inhibitory, influences of limbic system structures on resident neurosecretory neuron pools (Roland and Sawchenko, 1993; Boudaba et al., 1996). The limbic region, particularly the hippocampal formation, is enriched in corticosteroid receptor expression and has been strongly implicated in mediating glucocorticoid negative feedback effects on the HPA axis. Coupled with recent indications that steroid feedback effects are exerted selectively on AVP expression (Kovács et al., 2000), the present findings would seem to warrant additional consideration of the involvement of local GABAergic mechanisms in the feedback control of AVP expression (Acs et al., 1985).
Previous studies have shown that low doses (10 pmol) of BMI microinjected into the dorsomedial nucleus of the hypothalamus, which lies immediately caudal to the PVH, can have potent affects on PVH activity (De Novellis et al., 1995; DiMicco et al., 1996). Although the sphere of drug distribution observed in our experiments with [3H]NE (Fig. 1) does not necessarily generalize to that of BMI, the lack of detectable Fos-IR in the dorsomedial nucleus of BMI-injected rats makes it seem unlikely that the effects we observed are a result of a primary site of action other than in the PVH.
The effect of glutamate microinjection on PVH activity
Glutamate microinjection elicited surprisingly weak effects on plasma corticosterone levels and Fos expression within the PVH. This result was unexpected, because the PVH is known to express a variety of (mainly ionotropic) glutamate receptors (van den Pol et al., 1994;Aubry et al., 1996; Herman et al., 2000; Eyigor et al., 2001) and to receive a substantial glutamatergic innervation (van den Pol et al., 1990; van den pol, 1991). The role of glutamate in the regulation of PVH mechanisms is unsettled, and its effects on parvocellular neurosecretory neurons have not been thoroughly characterized (Oliver et al., 1996; Herman and Cullinan, 1997). For example, in vitro studies report that hypothalamic CRF release may be increased (Joanny et al., 1997) decreased, or unaffected (Costa et al., 1992; Patchev et al., 1994) by excitatory amino acid administration. Another reports that glutamate alone has no effect on hypothalamic cultures but can potentiate the effects of forskolin (Yang et al., 1995). Peripheral EAA administration results in increased ACTH and corticosterone levels (Jezova et al., 1991), but the site of action for this effect is undetermined (Oliver et al., 1996). Similarly conflicting results have been reported on the effects of local glutamate injection on autonomic (cardiovascular) parameters (Katafuchi et al., 1988; Darlington et al., 1989; Martin and Haywood, 1992;DiMicco et al., 1996). Several studies have reported that microinjection of glutamate directly into the PVH results in increased plasma ACTH and corticosterone (Darlington et al., 1989; Feldman and Weidenfeld, 1997). The basis for the apparent conflict between these and the present results is not entirely clear, although these previous studies were performed acutely in pentobarbital anesthetized rats, whereas ours were in awake behaving animals with implanted cannulas. Potentially, gliosis around chronically implanted cannulas could act as a sink to take up injected glutamate and dampen injection effects. This seems unlikely to account for the muted responses observed here, because our injections did produce a mild activation of the autonomic compartment of the PVH and a more pronounced response in select perinuclear areas. In addition, injections into the hippocampal formation using a similar approach provoked strong activational responses (Fig. 5).
Considerations of dosage may also be relevant to reconciling the disparate results concerning glutamatergic regulation of PVH mechanisms. It has been cautioned that, whereas microinjection of low doses (<5 nmol) of glutamate can produce excitation, higher doses can result in long-lasting inhibition as a consequence of depolarization block (Lipski et al., 1988). In our studies, doses of glutamate ranging from 100 pmol to 100 nmol produced comparable effects, and the primary (1 nmol) dose is within the range that would be expected to yield interpretable results.
Finally, the lack of glutamate effects may be attributable in part to concurrent stimulation of local inhibitory GABAergic neurons. We observed in control animals that a significant proportion of perinuclear Fos-positive cells displayed a marker of the GABAergic phenotype and that the number of such double-labeled cells was increased in response to local glutamate application. In view of anatomical evidence to support the existence of inputs to the parvocellular PVH from perinuclear GABA cells (Roland and Sawchenko, 1993) and electrophysiological work to indicate that such inhibitory interneurons are sensitive to glutamate (Tasker and Dudek, 1993;Boudaba et al., 1996), it is possible that activation of local circuit neurons by excitatory amino acids may serve to dampen direct neurotransmitter effects on populations intrinsic to the PVH. It remains to be determined to what extent local glutamate-sensitive GABAergic elements identified in our experiments actually contribute to the innervation of the PVH in general and to that of parvocellular neurosecretory neurons in particular. Current understanding of the distribution of glutamate receptors in and around the PVH is of limited value in ascertaining a likely site of action, because some are localized preferentially to the PVH, some to perinuclear regions, and still others distributed ubiquitously across both (Herman et al., 2000;Eyigor et al., 2001). In any event, the present findings call attention to the potential confounding influence of local circuit neurons in interpreting the effects of microinjection studies, which, in this instance, leave open to question the nature and any selectivity of glutamatergic regulation of PVH effector populations.
Footnotes
This work was supported by National Institutes of Health Grant NS-21182. We thank Drs. A. Ericsson, T. G. Sherman, and A. Tobin for generously providing plasmids and Dr. C. Rivier for RIAs. We gratefully acknowledge the assistance of Carlos Arias (technical), Kris Trulock (graphic–photographic), Belle Wamsley (editorial), and Eugene Brandon for helpful comments on this manuscript.
Correspondence should be addressed to Dr. P. E. Sawchenko, The Salk Institute, 10010 N. Torrey Pines Road, La Jolla, CA 92037. E-mail:sawchenko{at}salk.edu.
