Article Figures & Data
Figures
- Fig. 1.
Dual intracellular recordings from HS and CH cells. a, Responses to visual motion in front of both eyes along the PD and ND of the cells. b, Simultaneously occurring large-amplitude EPSPs in HS and CH cells.c, Rebound action potentials elicited in an HSE cell are not transmitted to the dCH cell. d, Response of a dCH cell to current injection into the ipsilateral HSE cell and vice versa.e, Current–voltage relationship between an HSN and a dCH cell. Current was injected into the HSN cell, and voltage response was measured in the dCH cell.
- Fig. 2.
Strength of connectivity between various pairs of HS and CH cells. a, Location of HS and CH cell dendrites within the lobula plate (modified from Borst and Haag, 1996).b, Current of either +10 nA (black bars) or −10 nA (gray bars) amplitude was injected into HS cells, and membrane voltage was measured in the dCH cell.c, Current was injected into the dCH cell, and membrane voltage was measured in HS cells. Data represent the mean ± SEM of four (HSN), five (HSE), and one (HSS) experiment.
- Fig. 3.
Optical imaging of connectivity between HS and CH cells. a, Anatomy of both cells (lens, Epiplan 10×, 0.20 NA). b–e, False-color images of relative change of fluorescence (ΔF/F) occurring in the dCH cell under various stimulus conditions (b,c, direct current injection into the dCH cell;d, e, current injection into the HSN cell). The dCH cell was filled with Calcium Green, and the HSN cell was filled with Alexa (lens, Zeiss UC 20×, 0.57 NA).
- Fig. 4.
Dendrotopic calcium accumulation in a vCH cell.a, Anatomy of a vCH cell filled with Calcium Green and an HSE and an HSS cell both filled with the red fluorescent dye Alexa.b–d, False-color images of the fluorescence change in the vCH cell. In c and d, the outlines of the HSE and HSS cells are superimposed in black. Also shown are the time courses of the relative change of fluorescence within the areas outlined in c andd during injection of depolarizing current into the HSE (c) and into the HSS (d) cell (lens, Zeiss UC 20×, 0.57 NA).
- Fig. 5.
Small local motion stimuli lead to locally confined dendritic activity in both HS and CH cells. a,b, Raw fluorescence images showing the anatomy of the cells tested. The vCH cell was filled with Calcium Green, and the HSS cell was filled with Calcium Orange. c, False-color images of the relative fluorescence change in both cells after local visual motion stimulation (12° × 12°). A Zeiss Epiplan 10×, 0.20 NA lens was used in the photographs of a, and aZeiss Epiplan 40×, 0.75 NA water immersion lens was used for the images in b and c. Color scale identical to Figure 3.
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