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Cellular/Molecular

Lighting up the Senses: FM1-43 Loading of Sensory Cells through Nonselective Ion Channels

Jason R. Meyers, Richard B. MacDonald, Anne Duggan, David Lenzi, David G. Standaert, Jeffrey T. Corwin and David P. Corey
Journal of Neuroscience 15 May 2003, 23 (10) 4054-4065; DOI: https://doi.org/10.1523/JNEUROSCI.23-10-04054.2003
Jason R. Meyers
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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Richard B. MacDonald
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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Anne Duggan
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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David Lenzi
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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David G. Standaert
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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Jeffrey T. Corwin
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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David P. Corey
1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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Article Information

DOI 
https://doi.org/10.1523/JNEUROSCI.23-10-04054.2003
PubMed 
12764092
Published By 
Society for Neuroscience
History 
  • Received January 2, 2003
  • Revision received March 4, 2003
  • Accepted March 7, 2003
  • First published May 15, 2003.
  • Version of record published May 15, 2003.
Copyright & Usage 
Copyright © 2003 Society for Neuroscience 0270-6474/03/234054-12.00/0

Author Information

  1. Jason R. Meyers1,*,
  2. Richard B. MacDonald3,*,
  3. Anne Duggan5,6,
  4. David Lenzi2,
  5. David G. Standaert4,7,
  6. Jeffrey T. Corwin2, and
  7. David P. Corey5,6
  1. 1Neuroscience Graduate Program and 2Departments of Otolaryngology-Head and Neck Surgery and Neuroscience, School of Medicine, University of Virginia, Charlottesville, Virginia 22908, 3Program in Speech and Hearing Sciences, Health Sciences and Technology Program, Massachusetts Institute of Technology, Boston, Massachusetts 02139, 4Departments of Neurology and, 5Neurobiology, Harvard Medical School, Boston, Massachusetts 02115, and Howard Hughes Medical Institute, 6Neurosurgery Service, and 7Neurology Service, Massachusetts General Hospital, Boston, Massachusetts 02114
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Author contributions

Disclosures

    • Received January 2, 2003.
    • Revision received March 4, 2003.
    • Accepted March 7, 2003.
  • ↵* J.R.M. and R.B. M. contributed equally to this work.

  • This work was supported by National Institute on Deafness and Other Communication Disorders Grants R01-DC00200 (J.T.C.) and R01-DC00304 (D.P.C.). J.R.M. is a predoctoral fellow, A.D. was an associate, and D.P.C. is an investigator of the Howard Hughes Medical Institute. We thank Baljit S. Khakh and Henry A. Lester (California Institute of Technology) for the P2X2 clone, Stefan Heller (Massachusetts Eye and Ear Infirmary) for the TRPV1 clone, Heidi L. Rehm and Gwénaëlle S. G. Géléoc [Massachusetts General Hospital (MGH)] for valuable advice and help with dissections, Ellen Lumpkin and David Julius(University of California San Francisco)for examining the P2X2 knock-out mouse, David Hill (University of Virginia) for helping to examine the taste system and for helpful discussions, Christine Kellar-McGandy (MGH) for brain sectioning, and Michael Whalen (MGH) for ventricle injections.

  • Correspondence should be addressed to Jason R Meyers, MR 4, Room 5148, HSC Box 801392, University of Virginia, Charlottesville, VA 22908. E-mail: jmeyers{at}virginia.edu.

  • A. Duggan's present address: Departments of Anesthesiology and Physiology, Northwestern University Institute for Neurosciences, Chicago, IL 60611.

  • D. Lenzi's present address: Cytokinetics Inc., South San Francisco, CA 94080.

  • Copyright © 2003 Society for Neuroscience 0270-6474/03/234054-12$15.00/0

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The Journal of Neuroscience: 23 (10)
Journal of Neuroscience
Vol. 23, Issue 10
15 May 2003
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Lighting up the Senses: FM1-43 Loading of Sensory Cells through Nonselective Ion Channels
Jason R. Meyers, Richard B. MacDonald, Anne Duggan, David Lenzi, David G. Standaert, Jeffrey T. Corwin, David P. Corey
Journal of Neuroscience 15 May 2003, 23 (10) 4054-4065; DOI: 10.1523/JNEUROSCI.23-10-04054.2003

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Lighting up the Senses: FM1-43 Loading of Sensory Cells through Nonselective Ion Channels
Jason R. Meyers, Richard B. MacDonald, Anne Duggan, David Lenzi, David G. Standaert, Jeffrey T. Corwin, David P. Corey
Journal of Neuroscience 15 May 2003, 23 (10) 4054-4065; DOI: 10.1523/JNEUROSCI.23-10-04054.2003
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Keywords

  • FM1-43
  • hair cell
  • vanilloid receptor
  • purinergic receptor
  • Merkel cells
  • DRG
  • nociceptors
  • TRP channels
  • sensory ganglia

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