Skip to main content

Main menu

  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE

User menu

  • Log out
  • Log in
  • My Cart

Search

  • Advanced search
Journal of Neuroscience
  • Log out
  • Log in
  • My Cart
Journal of Neuroscience

Advanced Search

Submit a Manuscript
  • HOME
  • CONTENT
    • Early Release
    • Featured
    • Current Issue
    • Issue Archive
    • Collections
    • Podcast
  • ALERTS
  • FOR AUTHORS
    • Information for Authors
    • Fees
    • Journal Clubs
    • eLetters
    • Submit
    • Special Collections
  • EDITORIAL BOARD
    • Editorial Board
    • ECR Advisory Board
    • Journal Staff
  • ABOUT
    • Overview
    • Advertise
    • For the Media
    • Rights and Permissions
    • Privacy Policy
    • Feedback
    • Accessibility
  • SUBSCRIBE
PreviousNext
Cellular/Molecular

Acid-Sensing Ion Channel 1 Is Localized in Brain Regions with High Synaptic Density and Contributes to Fear Conditioning

John A. Wemmie, Candice C. Askwith, Ejvis Lamani, Martin D. Cassell, John H. Freeman Jr and Michael J. Welsh
Journal of Neuroscience 2 July 2003, 23 (13) 5496-5502; https://doi.org/10.1523/JNEUROSCI.23-13-05496.2003
John A. Wemmie
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Candice C. Askwith
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ejvis Lamani
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Martin D. Cassell
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
John H. Freeman Jr
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michael J. Welsh
Departments of 1Psychiatry, 2Physiology and Biophysics, 3Internal Medicine, 4Anatomy and Cell Biology, and 5Psychology, and 6Neuroscience Graduate Program and 7Howard Hughes Medical Institute, University of Iowa, Iowa City, Iowa 52242 and 8Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Article Figures & Data

Figures

  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    ASIC1 immunolocalization in forebrain. A, Coronal sections were stained for Nissl substance or immunolabeled for ASIC1 protein in +/+ and –/– mice. Areas marked by dashed lines in the Nissl-stained section are the areas dissected to prepare protein extracts for Western blotting in D and Figure 6 B. Asterisks in ASIC1 +/+ hemisphere denote areas of nonspecific staining that did not occur bilaterally or in multiple sections. B, C, Enlarged images of dentate gyrus and CA1 respectively. D, Western blot of ASIC1 protein in 100μg protein extract from dentate gyrus and CA1. amg, amygdala; cc, corpus callosum; dg, dentate gyrus; ec, external capsule; ect, ectorhinal cortex; En, endopiriform nuclei; fi, fimbria; Hb, habenula; H, hilus (polymorphic layer); ic, internal capsule; LTh, lateral thalamus; MS, medial septal nuclei; PAC, parietal association cortex; Pir, piriform cortex; PCg, posterior cingulate cortex; PRh, perirhinal cortex; S1BF, somatosensory barrel field; Th, thalamus.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    ASIC1 immunolocalization in cortex. A, B, Immunolabeling in the posterior (post.) cingulate cortex. Stripes extending through layer II are labeled with an arrowhead. Positive-staining pyramidal cells in layer III are labeled with arrows. *ASIC1-specific staining in layer I. C, ASIC1 immunostaining is also elevated in layer III of barrel cortex.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Immunolocalization of ASIC1 in the sensorimotor cortex and striatum. Coronal sections through the forebrain were stained for Nissl substance, hematoxylin and eosin (H&E), or ASIC1 protein in ASIC1 +/+ or –/– mice. Center row, staining of representative coronal slices. Top row, insets of somatosensory cortex at higher magnification. Bottom row, insets of external capsule/corpus callosum and striatum at higher magnification. White matter tracts are labeled with arrows. ASIC1 immunolabeling was noticeably reduced in the white matter tracts. Areas of staining that were not present bilaterally and not present in multiple slices, suggesting nonspecific staining, are marked with an asterisk. aca, anterior commissure; Acb, accumbens nucleus; cc, corpus callosum; Cg, cingulate cortex; CPu, caudate/putamen (striatum); ec, external capsule; M1, primary motor cortex; Pir, piriform cortex; S1, somatosensory cortex; VP, ventral pallidum; Tu, olfactory tubercle.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Immunolocalization of ASIC1 in the olfactory bulb. Coronal sections through the olfactory bulb were stained for Nissl substance or immunolabeled for ASIC1 protein in ASIC1 +/+ and –/– mice. Higher magnifications at bottom demonstrate ASIC1 immunostaining in glomeruli (arrowheads). E/OV, ependymal and subendymal layer/olfactory ventricle; EPl, external plexiform layer; Gl, glomerular layer; Gr, granule cell layer; IPl, internal plexiform layer; Mi, mitral cell layer; ON, olfactory nerve layer.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Immunolocalization of ASIC1 in the cerebellum. ASIC1 immunohistochemistry in coronal (A) and parasagittal (B) sections of the cerebellum. C, Immunostaining with anti-calbindin D-28K antibody in fresh-frozen tissue. 4V, fourth ventricle; DN, deep cerebellar nuclei; Gc, granule cell layer; ML, molecular layer; Pc, pyramidal cell layer; WM, white matter.

  • Figure 6.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 6.

    A, ASIC1 immunolocalization in the amygdala complex. Bla, basolateral nucleus; Ce, central nucleus; La, lateral nucleus. B, Western blotting of ASIC1 protein in 100 μg of protein extract per lane isolated from indicated brain region. Cos-7 cells transfected with mASIC1, cos. Because the entire cerebellum was used to generate the cb extract, the subcortical structures with little ASIC1 may have diluted out the high expression level seen by immunohistological staining in the cerebellar cortex (Fig. 5). +/+ and –/–, whole-brain extract from ASIC1 +/+ or –/– mouse; amg, amygdala; cb, cerebellum; dg, dentate gyrus; Hb, habenula; S1, somatosensory barrel field; Th, thalamus; PAC, parietal association cortex; PCg, posterior cingulate cortex.

  • Figure 7.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 7.

    Proton-gated currents in amygdala neurons. A, B, Representative recordings of pH 5 evoked response in amygdala neurons from ASIC1 +/+ and –/– mice. C, Average current density of peak pH 5-evoked response in amygdala neurons from ASIC1 +/+ (n = 14) and –/– (n = 18) mice and hippocampal neurons from ASIC1 +/+ mice (n = 67; *p < 0.01).

  • Figure 8.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 8.

    Behavioral analysis of learned fear. A, B, Cued fear conditioning. The amount of freezing in 1 min intervals was determined during training (A) and testing (B). During testing, the ASIC1 –/– mice froze significantly less than +/+ controls with the presentation of the conditioned stimulus (intervals 4–9) (p = 0.02) (+/+, n = 5; –/–, n = 9). C, D, Context fear conditioning. The difference in freezing between +/+ and –/– mice was significant during training (intervals 4–6; p = 0.002) and during testing (p = 0.03) (+/+, n = 7; –/–,n=8). Footshock, arrows; tone, bars. Statistical significance was tested by ANOVA with repeated measures.

Back to top

In this issue

The Journal of Neuroscience: 23 (13)
Journal of Neuroscience
Vol. 23, Issue 13
2 Jul 2003
  • Table of Contents
  • About the Cover
  • Index by author
Email

Thank you for sharing this Journal of Neuroscience article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Acid-Sensing Ion Channel 1 Is Localized in Brain Regions with High Synaptic Density and Contributes to Fear Conditioning
(Your Name) has forwarded a page to you from Journal of Neuroscience
(Your Name) thought you would be interested in this article in Journal of Neuroscience.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Print
View Full Page PDF
Citation Tools
Acid-Sensing Ion Channel 1 Is Localized in Brain Regions with High Synaptic Density and Contributes to Fear Conditioning
John A. Wemmie, Candice C. Askwith, Ejvis Lamani, Martin D. Cassell, John H. Freeman Jr, Michael J. Welsh
Journal of Neuroscience 2 July 2003, 23 (13) 5496-5502; DOI: 10.1523/JNEUROSCI.23-13-05496.2003

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Respond to this article
Request Permissions
Share
Acid-Sensing Ion Channel 1 Is Localized in Brain Regions with High Synaptic Density and Contributes to Fear Conditioning
John A. Wemmie, Candice C. Askwith, Ejvis Lamani, Martin D. Cassell, John H. Freeman Jr, Michael J. Welsh
Journal of Neuroscience 2 July 2003, 23 (13) 5496-5502; DOI: 10.1523/JNEUROSCI.23-13-05496.2003
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results and Discussion
    • Conclusions
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Keywords

  • ASIC1
  • localization
  • CNS
  • fear conditioning
  • emotion
  • learning, memory

Responses to this article

Respond to this article

Jump to comment:

No eLetters have been published for this article.

Related Articles

Cited By...

More in this TOC Section

  • CXCL12 Engages Cortical Inhibitory Neurons to Enhance Dendritic Spine Plasticity and Structured Network Activity
  • Atypical Cadherin FAT2 Is Required for Synaptic Integrity and Motor Behaviors
  • Sex Differences in Histamine Regulation of Striatal Dopamine
Show more Cellular/Molecular
  • Home
  • Alerts
  • Follow SFN on BlueSky
  • Visit Society for Neuroscience on Facebook
  • Follow Society for Neuroscience on Twitter
  • Follow Society for Neuroscience on LinkedIn
  • Visit Society for Neuroscience on Youtube
  • Follow our RSS feeds

Content

  • Early Release
  • Current Issue
  • Issue Archive
  • Collections

Information

  • For Authors
  • For Advertisers
  • For the Media
  • For Subscribers

About

  • About the Journal
  • Editorial Board
  • Privacy Notice
  • Contact
  • Accessibility
(JNeurosci logo)
(SfN logo)

Copyright © 2025 by the Society for Neuroscience.
JNeurosci Online ISSN: 1529-2401

The ideas and opinions expressed in JNeurosci do not necessarily reflect those of SfN or the JNeurosci Editorial Board. Publication of an advertisement or other product mention in JNeurosci should not be construed as an endorsement of the manufacturer’s claims. SfN does not assume any responsibility for any injury and/or damage to persons or property arising from or related to any use of any material contained in JNeurosci.